Origin IGS: cccgatgcgtaaannnnnttaattaattaannnnngcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcnnnnnttaattaattaannnnntttacgcatcggg Mask Tandem Repeat Region ================================================ cccgatgcgtaaannnnnttaattaattaannnnngcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Find is-nt database================================================ Query_seq: SMT_325:SMT_326|SMT_325:SMT_326:K02029 polar amino acid transport system permease protein:K02029 polar amino acid transport system permease protein:<-<-:263114..263306 193 cccgatgcgtaaannnnnttaattaattaannnnngcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_325:SMT_326|SMT_325:SMT_326:K02029 polar amino acid transport system permease protein:K02029 polar amino acid transport system permease protein:<-<-:263114..263306 193 cccgatgcgtaaannnnnttaattaattaannnnngcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_325:SMT_326|SMT_325:SMT_326:K02029 polar amino acid transport system permease protein:K02029 polar amino acid transport system permease protein:<-<-:263114..263306 193 cccgatgcgtaaannnnnttaattaattaannnnngcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cccgatgcgtaaannnnnttaattaattaannnnngcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 26 End: 130 ......................... M X X X S R T T E R S E S V S E E A E E R P I R K P P L P A R W P I H ............................................................... Protein_Len: 43 Strand: - Start: 65 End: 193 ................................................................ H A L F R F L A W Y A F R R E G R T P R N M L A S S A H R C T S E L P D G Q A L I T M Protein_Len: 42 Strand: - Start: 30 End: 155 ............................. X X C G F S R A C R T L S R P L P L A G L V C V A E G R A N A S E N I C L K C A Q M ...................................... Protein_Len: 35 Strand: - Start: 22 End: 126 ..................... N I L X X L R V V S R L S D T L S S A S S R G I R L G G R G A R Q G M ................................................................... cccgatgcgtaaannnnnttaattaattaannnnngcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cccgatgcgtaaannnnnttaattaattaannnnngcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_325:SMT_326|SMT_325:SMT_326:K02029 polar amino acid transport system permease protein:K02029 polar amino acid transport system permease protein:<-<-:263114..263306 193 cccgatgcgtaaannnnnttaattaattaannnnngcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Intra-Species Hit: Count: 36 Min: 1 Max: 193 Len: 193 Subject: smit_SMT_325_SMT_326|K02029 polar amino acid transport system permease protein:K02029 polar amino acid transport system permease protein|NEGATIVE:NEGATIVE|[263114,263306]|193 HSP 1 e-value: 1.0E-87 bit: 323.0 Len: 193 Query Start:1 Query End:193 Subject Strand: POSITIVE Subject Start: 1 Subject End: 193 cccgatgcgtaaannnnnttaattaattaannnnngcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac cccgatgcgtaaannnnnttaattaattaannnnngcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_2024_SMT_2025|hypothetical protein:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1940751,1941084]|334 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: NEGATIVE Subject Start: 6 Subject End: 163 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_2016_SMT_2017|tyrosine recombinase:exonuclease RexA|NEGATIVE:NEGATIVE|[1933516,1935951]|2436 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: NEGATIVE Subject Start: 83 Subject End: 240 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1981_SMT_1982|K03696 ATP-dependent Clp protease ATP-binding subunit ClpC:conserved hypothetical protein|POSITIVE:POSITIVE|[1912929,1913175]|247 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: NEGATIVE Subject Start: 6 Subject End: 163 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1978_SMT_1979|hypothetical protein:possible cell surface protein|NEGATIVE:NEGATIVE|[1909657,1910371]|715 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: POSITIVE Subject Start: 448 Subject End: 605 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1967_SMT_1968|dicarboxylate/amino acid:cation (Na+ or H+) symporter (DAACS) family protein:transcription-repair coupling factor|NEGATIVE:NEGATIVE|[1899774,1900040]|267 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: NEGATIVE Subject Start: 19 Subject End: 176 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1966_SMT_1967|DNA polymerase III alpha subunit:dicarboxylate/amino acid:cation (Na+ or H+) symporter (DAACS) family protein|POSITIVE:NEGATIVE|[1898389,1899587]|1199 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: NEGATIVE Subject Start: 17 Subject End: 174 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac HSP 2 e-value: 2.0E-52 bit: 206.0 Len: 104 Query Start:36 Query End:139 Subject Strand: NEGATIVE Subject Start: 227 Subject End: 330 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcaga...................................................... ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcaga...................................................... Subject: smit_SMT_1953_SMT_1954|hypothetical protein:6-phospho-beta- glucosidase|POSITIVE:POSITIVE|[1888168,1888687]|520 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: POSITIVE Subject Start: 301 Subject End: 458 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1946_SMT_1947|sucrose-6-phosphate hydrolase:lysine decarboxylase|POSITIVE:NEGATIVE|[1881266,1882402]|1137 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: NEGATIVE Subject Start: 493 Subject End: 650 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1944_SMT_1945|hypothetical protein predicted by Glimmer/Critica:putative dihydrolipoamide S-acetyltransferase|NEGATIVE:POSITIVE|[1879585, 1880100]|516 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: NEGATIVE Subject Start: 54 Subject End: 211 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1926_SMT_1927|mevalonate kinase:cell division protein FtsH|POSITIVE:NEGATIVE|[1868123,1868497]|375 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: NEGATIVE Subject Start: 6 Subject End: 163 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac HSP 2 e-value: 2.0E-83 bit: 309.0 Len: 156 Query Start:38 Query End:193 Subject Strand: POSITIVE Subject Start: 204 Subject End: 359 .....................................agccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac .....................................agccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1895_SMT_1896|hypothetical protein predicted by Glimmer/Critica:lysine decarboxylase|POSITIVE:NEGATIVE|[1847463,1848162]|700 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: NEGATIVE Subject Start: 78 Subject End: 235 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1887_SMT_1888|LicC protein:K02026 multiple sugar transport system permease protein|POSITIVE:POSITIVE|[1841801,1842227]|427 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: NEGATIVE Subject Start: 6 Subject End: 163 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1885_SMT_1886|hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1840148,1841123]|976 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: POSITIVE Subject Start: 601 Subject End: 758 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1884_SMT_1885|hypothetical protein:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1839299,1839919]|621 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: POSITIVE Subject Start: 448 Subject End: 605 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1873_SMT_1874|K02006 cobalt transport system ATP-binding protein:hypothetical protein|POSITIVE:NEGATIVE|[1832412,1832712]|301 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: NEGATIVE Subject Start: 17 Subject End: 174 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1857_SMT_1858|hypothetical protein:K03497 chromosome partitioning protein, ParB family|POSITIVE:NEGATIVE|[1821206,1821776]|571 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: NEGATIVE Subject Start: 93 Subject End: 250 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1851_SMT_1852|ABC transporter, permease protein, putative:1,4-alpha-glucan branching enzyme|NEGATIVE:NEGATIVE|[1815812,1816556]|745 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: POSITIVE Subject Start: 588 Subject End: 745 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1849_SMT_1850|hypothetical protein:K01421 putative membrane protein|POSITIVE:POSITIVE|[1813865,1815201]|1337 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: POSITIVE Subject Start: 820 Subject End: 977 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1690_SMT_1691|K06148 ATP-binding cassette, subfamily C, bacterial:putative deoxyribonuclease|NEGATIVE:POSITIVE|[1674870, 1675117]|248 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: NEGATIVE Subject Start: 19 Subject End: 176 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1685_SMT_1686|phenylalanyl-tRNA synthetase alpha chain:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1668936,1670314]|1379 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: POSITIVE Subject Start: 1128 Subject End: 1285 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_779_SMT_780|ribonuclease BN, putative:peptidase family U32|NEGATIVE:NEGATIVE|[688621,688841]|221 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: NEGATIVE Subject Start: 19 Subject End: 176 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_478_SMT_479|hypothetical protein predicted by Glimmer/Critica:K03308 neurotransmitter:Na+ symporter, NSS family|POSITIVE:NEGATIVE|[415318,415693]|376 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 158 Query Start:36 Query End:193 Subject Strand: POSITIVE Subject Start: 150 Subject End: 307 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1971_SMT_1972|hypothetical protein predicted by Glimmer/Critica:uncharacterized conserved phage related protein|POSITIVE:POSITIVE|[1903587,1903801]|215 HSP 1 e-value: 3.0E-82 bit: 305.0 Len: 158 Query Start:36 Query End:193 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 158 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcttctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1845_SMT_1846|K01191 alpha-mannosidase:acyl-ACP thioesterase, putative|POSITIVE:POSITIVE|[1810496,1811549]|1054 HSP 1 e-value: 4.0E-81 bit: 301.0 Len: 158 Query Start:36 Query End:193 Subject Strand: POSITIVE Subject Start: 534 Subject End: 691 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaarscgcctmtccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_303_SMT_304|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[245819,246719]|901 HSP 1 e-value: 7.0E-80 bit: 297.0 Len: 150 Query Start:36 Query End:185 Subject Strand: POSITIVE Subject Start: 752 Subject End: 901 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctagg........ ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctagg........ HSP 2 e-value: 2.0E-37 bit: 157.0 Len: 83 Query Start:111 Query End:193 Subject Strand: NEGATIVE Subject Start: 137 Subject End: 219 ..............................................................................................................ccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ..............................................................................................................ccgcgcgttggccgattcataaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1558_SMT_1559|hypothetical protein:6- phosphofructokinase|NEGATIVE:NEGATIVE|[1544149, 1544703]|555 HSP 1 e-value: 1.0E-78 bit: 293.0 Len: 158 Query Start:36 Query End:193 Subject Strand: NEGATIVE Subject Start: 209 Subject End: 365 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaa-ccgcctctccccgcgcgttggccgattcattaatgcrgagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1942_SMT_1943|zinc metalloprotease ZmpB, truncated:alpha/beta hydrolase fold|POSITIVE:NEGATIVE|[1878445,1878659]|215 HSP 1 e-value: 4.0E-69 bit: 262.0 Len: 132 Query Start:62 Query End:193 Subject Strand: NEGATIVE Subject Start: 6 Subject End: 137 .............................................................tcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac .............................................................tcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_2029_SMT_2030|acetyltransferase, GNAT family:chromosomal replication initiator protein DnaA|POSITIVE:NEGATIVE|[1944345,1944879]|535 HSP 1 e-value: 1.0E-65 bit: 250.0 Len: 126 Query Start:68 Query End:193 Subject Strand: POSITIVE Subject Start: 337 Subject End: 462 ...................................................................agcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................................................agcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1985_SMT_1986|hypothetical protein predicted by Glimmer/Critica:mercuric reductase|NEGATIVE:POSITIVE|[1914662,1914982]|321 HSP 1 e-value: 1.0E-62 bit: 240.0 Len: 121 Query Start:73 Query End:193 Subject Strand: NEGATIVE Subject Start: 162 Subject End: 282 ........................................................................ggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ........................................................................ggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_2001_SMT_2002|membrane protease protein family:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1923950,1924234]|285 HSP 1 e-value: 1.0E-59 bit: 230.0 Len: 120 Query Start:74 Query End:193 Subject Strand: POSITIVE Subject Start: 150 Subject End: 269 .........................................................................gaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac .........................................................................gaagcggaagagcgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_2000_SMT_2001|hypothetical protein:membrane protease protein family|NEGATIVE:POSITIVE|[1923199,1923526]|328 HSP 1 e-value: 8.0E-58 bit: 224.0 Len: 113 Query Start:81 Query End:193 Subject Strand: POSITIVE Subject Start: 143 Subject End: 255 ................................................................................aagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ................................................................................aagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_387_SMT_388|hypothetical protein predicted by Glimmer/Critica:branched-chain amino acid transport system II carrier protein|POSITIVE:POSITIVE|[321179,321311]|133 HSP 1 e-value: 5.0E-56 bit: 218.0 Len: 110 Query Start:84 Query End:193 Subject Strand: POSITIVE Subject Start: 6 Subject End: 115 ...................................................................................agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ...................................................................................agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_1955_SMT_1956|carbamoyl-phosphate synthase small chain:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:NEGATIVE|[1889499,1889626]|128 HSP 1 e-value: 1.0E-50 bit: 200.0 Len: 105 Query Start:89 Query End:193 Subject Strand: NEGATIVE Subject Start: 19 Subject End: 123 ........................................................................................ccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac ........................................................................................ccaatacgcaaaccgcttctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_2044_SMT_2045|transcriptional activator TipA, putative:K+ transport system, NAD-binding component|NEGATIVE:POSITIVE|[1955976,1956278]|303 HSP 1 e-value: 4.0E-44 bit: 178.0 Len: 106 Query Start:88 Query End:193 Subject Strand: NEGATIVE Subject Start: 98 Subject End: 203 .......................................................................................cccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac .......................................................................................cccaatacgcaaaccgcctctccccgcgcgtgggcgatttcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Subject: smit_SMT_2005_SMT_2006|pullulanase, type I:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1926380,1926611]|232 HSP 1 e-value: 3.0E-39 bit: 163.0 Len: 82 Query Start:36 Query End:117 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 82 ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcg............................................................................ ...................................gcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcg............................................................................ HSP 2 e-value: 2.0E-6 bit: 54.0 Len: 27 Query Start:167 Query End:193 Subject Strand: POSITIVE Subject Start: 109 Subject End: 135 ......................................................................................................................................................................aggatccccctgtgctaggatggtcac ......................................................................................................................................................................aggatccccctgtgctaggatggtcac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CCCGAUGCGUAAANNNNNUUAAUUAAUUAANNNNNGCAGCCGAACGACCGAGCGCAGCGAGUCAGUGAGCGAGGAAGCGGAAGAGCGCCCAAUACGCAAACCGCCUCUCCCCGCGCGUUGGCCGAUUCAUUAAUGCAGAGCUUGCAUGCCUGCAGGUCGACUCUAGAGGAUCCCCCUGUGCUAGGAUGGUCAC ..((.(((...........................)))..))...((((((((((((.(.(((.....(((.(((.((((....(((.......)))...))))...))).)))..(((((((..........(((((.((......)))))))))))))).......)))..).)))))))....))))).. (-54.03) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUGACCAUCCUAGCACAGGGGGAUCCUCUAGAGUCGACCUGCAGGCAUGCAAGCUCUGCAUUAAUGAAUCGGCCAACGCGCGGGGAGAGGCGGUUUGCGUAUUGGGCGCUCUUCCGCUUCCUCGCUCACUGACUCGCUGCGCUCGGUCGUUCGGCUGCNNNNNUUAAUUAAUUAANNNNNUUUACGCAUCGGG ........((..((..(((((...)))))..((((((..(((((((......)).))))).........(((((...((((((.(((...((((..(((....(((.((......)).))).)))..)))).))).))))))..))))).)))))).............................))...)). (-59.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1196 90 41 230 181 smit:SMT_1982|5end_conserved_hypothetical_protein_1913036..1913266_POSITIVE 1196 90 41 203 154 smit:SMT_1972|5end_uncharacterized_conserved_phage_related_protein_1903662..1903892_POSITIVE 1196 90 41 218 169 smit:SMT_1691|5end_putative_deoxyribonuclease_1674978..1675208_POSITIVE 1196 90 41 181 132 smit:SMT_325|5end_K02029_polar_amino_acid_transport_system_permease_protein_263023..263253_NEGATIVE 1137 130 81 188 139 smit:SMT_1986|5end_mercuric_reductase_1914843..1915073_POSITIVE 1019 190 141 195 146 smit:SMT_959|5end_1-acyl-sn-glycerol-3-phosphate_acyltransferase_905283..905513_POSITIVE 1009 78 36 231 189 smit:SMT_1156|5end_K07485_transposase_1099938..1100168_POSITIVE 979 169 122 231 184 smit:SMT_1914|5end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1859486..1859716_POSITIVE 775 127 88 230 192 smit:SMT_2045|5end_K+_transport_system,_NAD-binding_component_1956139..1956369_POSITIVE 477 190 167 223 200 smit:SMT_2005|5end_pullulanase,_type_I_1926289..1926519_NEGATIVE 427 89 41 136 94 smit:SMT_2052|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1961591..1961821_NEGATIVE 408 160 113 180 131 smit:SMT_110|5end_alpha-L-fucosidase_57445..57675_POSITIVE 380 86 42 164 119 smit:SMT_125|5end_hypothetical_protein_77664..77894_NEGATIVE 377 90 48 174 123 smit:SMT_1887|5end_LicC_protein_1841445..1841675_POSITIVE 373 106 64 44 3 smit:SMT_629|5end_glycogen_synthase_533967..534197_POSITIVE 371 159 113 142 88 smit:SMT_941|5end_hypothetical_protein_893558..893788_NEGATIVE 367 129 81 212 156 smit:SMT_1626|5end_undecaprenyl-diphosphatase_1616028..1616258_NEGATIVE 361 89 45 193 141 smit:SMT_890|5end_K02029_polar_amino_acid_transport_system_permease_protein_836953..837183_POSITIVE 352 116 76 208 167 smit:SMT_2002|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1924405..1924635_NEGATIVE 352 116 76 208 167 smit:SMT_1942|5end_zinc_metalloprotease_ZmpB,_truncated_1877882..1878112_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1097 190 141 82 33 smit:SMT_2024|3end_hypothetical_protein_1940690..1940840_POSITIVE 1097 190 141 71 22 smit:SMT_2002|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1924145..1924295_NEGATIVE 1097 190 141 82 33 smit:SMT_1981|3end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1912868..1913018_POSITIVE 1097 190 141 87 38 smit:SMT_1971|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1903526..1903676_POSITIVE 1097 190 141 71 22 smit:SMT_1966|3end_DNA_polymerase_III_alpha_subunit_1898328..1898478_POSITIVE 1097 190 141 82 33 smit:SMT_1942|3end_zinc_metalloprotease_ZmpB,_truncated_1878384..1878534_POSITIVE 1097 190 141 71 22 smit:SMT_1927|3end_cell_division_protein_FtsH_1868408..1868558_NEGATIVE 1097 190 141 82 33 smit:SMT_1926|3end_mevalonate_kinase_1868062..1868212_POSITIVE 1097 190 141 82 33 smit:SMT_1887|3end_LicC_protein_1841740..1841890_POSITIVE 1097 190 141 71 22 smit:SMT_1885|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1839830..1839980_NEGATIVE 1097 190 141 71 22 smit:SMT_1873|3end_K02006_cobalt_transport_system_ATP-binding_protein_1832351..1832501_POSITIVE 1097 190 141 87 38 smit:SMT_1852|3end_1,4-alpha-glucan_branching_enzyme_1816467..1816617_NEGATIVE 1019 190 141 71 22 smit:SMT_958|3end_hypothetical_protein_predicted_by_Glimmer/Critica_905239..905389_POSITIVE 395 78 37 83 39 smit:SMT_481|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_417641..417791_NEGATIVE 373 106 64 47 6 smit:SMT_628|3end_glucose-1-phosphate_adenylyltransferase,_GlgD_subunit_534050..534200_POSITIVE 367 129 81 151 95 smit:SMT_1627|3end_hypothetical_protein_1616089..1616239_NEGATIVE 344 180 133 77 16 smit:SMT_1523|3end_ferrochelatase_1503390..1503540_NEGATIVE 343 122 82 66 19 smit:SMT_1941|3end_hypothetical_protein_1877663..1877813_POSITIVE 337 190 173 18 1 smit:SMT_479|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_415604..415754_NEGATIVE 332 156 113 83 31 smit:SMT_357|3end_tRNA_(guanine-N7-)-methyltransferase_293900..294050_POSITIVE