Origin IGS: gtttttacctctttaataaatattattactcttcattttaccataaaatcttctggaaataaagccaaaatagttattgggagatggtaggggagaaaaagaatattgcctagtcaggtggagtggttaatgttaaagagtatctaccatatttcttctcatactcaatggaaatcaaagagcaaactaggaagcaagccgcaggttgctcaaaacactgttttgaggttgtggatagaactgacgaagtcagtaaccatatatacggtaacgtgacgctgacgtggtttgaagagattttcgaagagtattatatttcaaaaggccgaagtaagcttactccgacctgttagtattgtcttaaaaagattgattt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaatcaatctttttaagacaatactaacaggtcggagtaagcttacttcggccttttgaaatataatactcttcgaaaatctcttcaaaccacgtcagcgtcacgttaccgtatatatggttactgacttcgtcagttctatccacaacctcaaaacagtgttttgagcaacctgcggcttgcttcctagtttgctctttgatttccattgagtatgagaagaaatatggtagatactctttaacattaaccactccacctgactaggcaatattctttttctcccctaccatctcccaataactattttggctttatttccagaagattttatggtaaaatgaagagtaataatatttattaaagaggtaaaaac Mask Tandem Repeat Region ================================================ gtttttacctctttaataaatattattactcttcattttaccataaaatcttctggaaataaagccaaaatagttattgggagatggtaggggagaaaaagaatattgcctagtcaggtggagtggttaatgttaaagagtatctaccatatttcttctcatactcaatggaaatcaaagagcaaactaggaagcaagccgcaggttgctcaaaacactgttttgaggttgtggatagaactgacgaagtcagtaaccatatatacggtaacgtgacgctgacgtggtttgaagagattttcgaagagtattatatttcaaaaggccgaagtaagcttactccgacctgttagtattgtcttaaaaagattgattt Find is-nt database================================================ Query_seq: SMT_319:SMT_320|SMT_319:SMT_320:K02356 elongation factor EF-P:alcohol dehydrogenase, zinc-containing:<-<-:258955..259330 376 gtttttacctctttaataaatattattactcttcattttaccataaaatcttctggaaataaagccaaaatagttattgggagatggtaggggagaaaaagaatattgcctagtcaggtggagtggttaatgttaaagagtatctaccatatttcttctcatactcaatggaaatcaaagagcaaactaggaagcaagccgcaggttgctcaaaacactgttttgaggttgtggatagaactgacgaagtcagtaaccatatatacggtaacgtgacgctgacgtggtttgaagagattttcgaagagtattatatttcaaaaggccgaagtaagcttactccgacctgttagtattgtcttaaaaagattgattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_319:SMT_320|SMT_319:SMT_320:K02356 elongation factor EF-P:alcohol dehydrogenase, zinc-containing:<-<-:258955..259330 376 gtttttacctctttaataaatattattactcttcattttaccataaaatcttctggaaataaagccaaaatagttattgggagatggtaggggagaaaaagaatattgcctagtcaggtggagtggttaatgttaaagagtatctaccatatttcttctcatactcaatggaaatcaaagagcaaactaggaagcaagccgcaggttgctcaaaacactgttttgaggttgtggatagaactgacgaagtcagtaaccatatatacggtaacgtgacgctgacgtggtttgaagagattttcgaagagtattatatttcaaaaggccgaagtaagcttactccgacctgttagtattgtcttaaaaagattgattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_319:SMT_320|SMT_319:SMT_320:K02356 elongation factor EF-P:alcohol dehydrogenase, zinc-containing:<-<-:258955..259330 376 gtttttacctctttaataaatattattactcttcattttaccataaaatcttctggaaataaagccaaaatagttattgggagatggtaggggagaaaaagaatattgcctagtcaggtggagtggttaatgttaaagagtatctaccatatttcttctcatactcaatggaaatcaaagagcaaactaggaagcaagccgcaggttgctcaaaacactgttttgaggttgtggatagaactgacgaagtcagtaaccatatatacggtaacgtgacgctgacgtggtttgaagagattttcgaagagtattatatttcaaaaggccgaagtaagcttactccgacctgttagtattgtcttaaaaagattgattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 11 Min: 152 Max: 317 Len: 166 Subject: UniRef90_Q04I61 Cluster: Hypothetical protein; n=1; Streptococcus pneumoniae D39|Rep: Hypothetical protein - Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) HSP 1 e-value: 9.0E-19 bit: 94.7 Len: 156 Query Start:156 Query End:311 Subject Strand: null Subject Start: 22 Subject End: 73 ........................................................................................................................................................... F S Y S M E I K E Q T R K Q A A G C S K H C F E V V D R T D E V S N H I Y G N V T L T W F E E I F E E Y ................................................................. ........................................................................................................................................................... F L Y S M K I K V Q T R K L A A G C S K H C F E V V D E T D E V S N H T Y G K A T L T W F E E I F E K Y ................................................................. Subject: UniRef90_Q04IR5 Cluster: Hypothetical protein; n=1; Streptococcus pneumoniae D39|Rep: Hypothetical protein - Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) HSP 1 e-value: 4.0E-18 bit: 92.4 Len: 144 Query Start:168 Query End:311 Subject Strand: null Subject Start: 1 Subject End: 48 ....................................................................................................................................................................... M E I K E Q T R K Q A A G C S K H C F E V V D R T D E V S N H I Y G N V T L T W F E E I F E E Y ................................................................. ....................................................................................................................................................................... M K I K E Q T R K L A A G C S K H C F E V V D E T D E V S N H T Y G K V K L T W F E E I F E E Y ................................................................. Subject: UniRef90_Q04M07 Cluster: Hypothetical protein; n=2; Streptococcus pneumoniae D39|Rep: Hypothetical protein - Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) HSP 1 e-value: 5.0E-16 bit: 85.5 Len: 144 Query Start:168 Query End:311 Subject Strand: null Subject Start: 1 Subject End: 48 ....................................................................................................................................................................... M E I K E Q T R K Q A A G C S K H C F E V V D R T D E V S N H I Y G N V T L T W F E E I F E E Y ................................................................. ....................................................................................................................................................................... M K I K E Q T R K L A A G C S K Q C F E V V D E T D E V S N H T Y G K A K L M W F E E I F E E Y ................................................................. Subject: UniRef90_Q04LB4 Cluster: Hypothetical protein; n=1; Streptococcus pneumoniae D39|Rep: Hypothetical protein - Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) HSP 1 e-value: 1.0E-15 bit: 84.3 Len: 144 Query Start:168 Query End:311 Subject Strand: null Subject Start: 1 Subject End: 63 ....................................................................................................................................................................... M E I K E Q T R K Q A A G C S K H C F E V V D R T D E V S - - - - - - - - - - - - - - - N H I Y G N V T L T W F E E I F E E Y ................................................................. ....................................................................................................................................................................... M K I K E Q T R K L A A G C S K H C F E V V D R T D E V S S K H C F E V V D E T D E V S N H T Y G R A T M T W F E E I F E E Y ................................................................. Subject: UniRef90_Q04KB8 Cluster: Hypothetical protein; n=1; Streptococcus pneumoniae D39|Rep: Hypothetical protein - Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) HSP 1 e-value: 3.0E-15 bit: 82.8 Len: 144 Query Start:168 Query End:311 Subject Strand: null Subject Start: 1 Subject End: 48 ....................................................................................................................................................................... M E I K E Q T R K Q A A G C S K H C F E V V D R T D E V S N H I Y G N V T L T W F E E I F E E Y ................................................................. ....................................................................................................................................................................... M K I K E Q T R K L A A G Y S K H S F E V V D E T D E V S N H T Y S K A T L T W F E E I F E E Y ................................................................. Subject: UniRef90_Q04L28 Cluster: Hypothetical protein; n=2; Streptococcus pneumoniae D39|Rep: Hypothetical protein - Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) HSP 1 e-value: 5.0E-13 bit: 75.5 Len: 144 Query Start:168 Query End:311 Subject Strand: null Subject Start: 1 Subject End: 63 ....................................................................................................................................................................... M E I K E Q T R K Q A A G C S K H C F E V - - - - - - - - - - - - - - - V D R T D E V S N H I Y G N V T L T W F E E I F E E Y ................................................................. ....................................................................................................................................................................... M K I K E Q T R K L A A G C S K H C F E V A D K T D E V S S K H G F E V V D E T D E V S N H T Y G K A T L T R I E E I F E E Y ................................................................. Subject: UniRef90_Q04I51 Cluster: Hypothetical protein; n=1; Streptococcus pneumoniae D39|Rep: Hypothetical protein - Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) HSP 1 e-value: 3.0E-12 bit: 72.8 Len: 129 Query Start:168 Query End:296 Subject Strand: null Subject Start: 1 Subject End: 58 ....................................................................................................................................................................... M E I K E Q T R K Q A A G C S K H C F E V V D R - - - - - - - - - - - - - - - T D E V S N H I Y G N V T L T W F E E ................................................................................ ....................................................................................................................................................................... M K I K E Q T R K L A A G C S K H C F E V M D R T D E V S S K H G F E V V D E T D E V S N H T Y G E V K L T W F E E ................................................................................ Subject: UniRef90_Q04LP5 Cluster: Hypothetical protein; n=1; Streptococcus pneumoniae D39|Rep: Hypothetical protein - Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) HSP 1 e-value: 9.0E-11 bit: 68.2 Len: 102 Query Start:210 Query End:311 Subject Strand: null Subject Start: 162 Subject End: 195 ................................................................................................................................................................................................................. S K H C F E V V D R T D E V S N H I Y G N V T L T W F E E I F E E Y ................................................................. ................................................................................................................................................................................................................. S K H C F E V V D R T D E V S N H T H G K A T L T W F E E I F K E Y ................................................................. HSP 2 e-value: 7.0E-7 bit: 55.1 Len: 96 Query Start:162 Query End:257 Subject Strand: null Subject Start: 26 Subject End: 57 ................................................................................................................................................................. Y S M E I K E Q T R K Q A A G C S K H C F E V V D R T D E V S N ....................................................................................................................... ................................................................................................................................................................. Y S M K T K E Q T R K L A S G C S K H C F E V V D G T D V V S S ....................................................................................................................... Subject: UniRef90_Q04I45 Cluster: Hypothetical protein; n=2; Streptococcus pneumoniae|Rep: Hypothetical protein - Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) HSP 1 e-value: 1.0E-10 bit: 67.4 Len: 117 Query Start:168 Query End:284 Subject Strand: null Subject Start: 1 Subject End: 39 ....................................................................................................................................................................... M E I K E Q T R K Q A A G C S K H C F E V V D R T D E V S N H I Y G N V T L T ............................................................................................ ....................................................................................................................................................................... M K I K G Q T R K L A A G C S K Q C F E V M D R T D E V S N H T Y G K A T L T ............................................................................................ Subject: UniRef90_Q04IH0 Cluster: Hypothetical protein; n=1; Streptococcus pneumoniae D39|Rep: Hypothetical protein - Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) HSP 1 e-value: 2.0E-9 bit: 63.5 Len: 102 Query Start:210 Query End:311 Subject Strand: null Subject Start: 9 Subject End: 42 ................................................................................................................................................................................................................. S K H C F E V V D R T D E V S N H I Y G N V T L T W F E E I F E E Y ................................................................. ................................................................................................................................................................................................................. S K H V F E V V D E T D E V S N H T Y G K A T L T W F A E I F E E Y ................................................................. Subject: UniRef90_O05164 Cluster: Hypothetical protein; n=1; Streptococcus pneumoniae|Rep: Hypothetical protein - Streptococcus pneumoniae HSP 1 e-value: 4.0E-6 bit: 44.7 Len: 72 Query Start:152 Query End:223 Subject Strand: null Subject Start: 24 Subject End: 47 ....................................................................................................................................................... K Q C F E Q P A A C F L V C S L I S I E Y E K K ......................................................................................................................................................... ....................................................................................................................................................... K Q C F E Q P A A S F L V C S L I F I E Y K N K ......................................................................................................................................................... HSP 2 e-value: 4.0E-6 bit: 27.7 Len: 39 Query Start:279 Query End:317 Subject Strand: null Subject Start: 5 Subject End: 17 ...................................................................................................................................................................................................................................................................................... N I I L F E N L F K P R Q ........................................................... ...................................................................................................................................................................................................................................................................................... D L I L F E N L F K P R Q ........................................................... gtttttacctctttaataaatattattactcttcattttaccataaaatcttctggaaataaagccaaaatagttattgggagatggtaggggagaaaaagaatattgcctagtcaggtggagtggttaatgttaaagagtatctaccatannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcaaaaggccgaagtaagcttactccgacctgttagtattgtcttaaaaagattgattt Predict ORF larger than 30AA ================================================ Protein_Len: 57 Strand: + Start: 103 End: 273 ...................................................................................................... M L P S Q V E W L M L K S I Y H I S S H T Q W K S K S K L G S K P Q V A Q N T V L R L W I E L T K S V T I Y T V T ....................................................................................................... Protein_Len: 60 Strand: + Start: 123 End: 350 .......................................................................................................................... M V N V K E Y L P Y F F S Y S M E I K E Q T R K Q A A G C S K H C F E V V D R T D E V S N H I Y G N V T L T W F E E I F .......................... Protein_Len: 34 Strand: - Start: 213 End: 314 .................................................................................................................................................................................................................... F V T K L N H I S S V F D T V M Y V T V H R Q R P K F L N E F L I M .............................................................. Protein_Len: 60 Strand: - Start: 113 End: 298 ................................................................................................................ P T T L T L S Y R G Y K K E Y E I S I L S C V L F C A A P Q E F C Q K S T T S L V S S T L L W I Y P L T V S V H N S S M .............................................................................. Protein_Len: 34 Strand: - Start: 16 End: 117 ............... Y I N N S K M K G Y F R R S I F G F Y N N P S P L P S F S Y Q R T M ................................................................................................................................................................................................................................................................... gtttttacctctttaataaatattattactcttcattttaccataaaatcttctggaaataaagccaaaatagttattgggagatggtaggggagaaaaagaatattgcctagtcaggtggagtggttaatgttaaagagtatctaccatannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcaaaaggccgaagtaagcttactccgacctgttagtattgtcttaaaaagattgattt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 35 End: 64 ..................................CTTTATTTCCAGAAGATTTTATGGTAAAAT........................................................................................................................................................................................................................................................................................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gtttttacctctttaataaatattattactcttcattttaccataaaatcttctggaaataaagccaaaatagttattgggagatggtaggggagaaaaagaatattgcctagtcaggtggagtggttaatgttaaagagtatctaccatannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcaaaaggccgaagtaagcttactccgacctgttagtattgtcttaaaaagattgattt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 76 HP_score: -10.4 Tail_Score: -2.61383 Start: 209 End: 227 Full_Region: GCAAGCCGCAGGTTG CTCAAAAC ACT GTTTTGAG GTTGTGGATAGAACT ................................................................................................................................................................................................................CTCAAAACACTGTTTTGAG..................................................................................................................................................... Find igs database================================================ Query_seq: SMT_319:SMT_320|SMT_319:SMT_320:K02356 elongation factor EF-P:alcohol dehydrogenase, zinc-containing:<-<-:258955..259330 376 gtttttacctctttaataaatattattactcttcattttaccataaaatcttctggaaataaagccaaaatagttattgggagatggtaggggagaaaaagaatattgcctagtcaggtggagtggttaatgttaaagagtatctaccatannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcaaaaggccgaagtaagcttactccgacctgttagtattgtcttaaaaagattgattt Intra-Species Hit: Count: 1 Min: 1 Max: 376 Len: 376 Subject: smit_SMT_319_SMT_320|K02356 elongation factor EF-P:alcohol dehydrogenase, zinc-containing|NEGATIVE:NEGATIVE|[258955,259330]|376 HSP 1 e-value: 4.0E-80 bit: 299.0 Len: 151 Query Start:1 Query End:151 Subject Strand: POSITIVE Subject Start: 1 Subject End: 151 gtttttacctctttaataaatattattactcttcattttaccataaaatcttctggaaataaagccaaaatagttattgggagatggtaggggagaaaaagaatattgcctagtcaggtggagtggttaatgttaaagagtatctaccata................................................................................................................................................................................................................................. gtttttacctctttaataaatattattactcttcattttaccataaaatcttctggaaataaagccaaaatagttattgggagatggtaggggagaaaaagaatattgcctagtcaggtggagtggttaatgttaaagagtatctaccata................................................................................................................................................................................................................................. HSP 2 e-value: 3.0E-25 bit: 117.0 Len: 59 Query Start:318 Query End:376 Subject Strand: POSITIVE Subject Start: 318 Subject End: 376 .............................................................................................................................................................................................................................................................................................................................tcaaaaggccgaagtaagcttactccgacctgttagtattgtcttaaaaagattgattt .............................................................................................................................................................................................................................................................................................................................tcaaaaggccgaagtaagcttactccgacctgttagtattgtcttaaaaagattgattt Inter-species Hit: Count: 3 Min: 1 Max: 100 Len: 100 Subject: NC_008533_SPD_0395_SPD_0396|translation elongation factor P:glu-trnagln amidotransferase subunit b|NEGATIVE:NEGATIVE|[396340,396467]|128 HSP 1 e-value: 8.0E-38 bit: 159.0 Len: 100 Query Start:1 Query End:100 Subject Strand: POSITIVE Subject Start: 1 Subject End: 100 gtttttacctctttaataaatattattactcttcattttaccataaaatcttctggaaataaagccaaaatagttattgggagatggtaggggagaaaaa.................................................................................................................................................................................................................................................................................... gtttttacctctttaataaatattattactcttcattttaccataaaatactctggaaataaagccaaaatagttattgggagttggtagagaagaaaaa.................................................................................................................................................................................................................................................................................... Subject: NC_003098_spr0392_spr0393|elongation factor P:aspartyl/glutamyl-tRNA amidotransferase subunit B|NEGATIVE:NEGATIVE|[388811,388938]|128 HSP 1 e-value: 8.0E-38 bit: 159.0 Len: 100 Query Start:1 Query End:100 Subject Strand: POSITIVE Subject Start: 1 Subject End: 100 gtttttacctctttaataaatattattactcttcattttaccataaaatcttctggaaataaagccaaaatagttattgggagatggtaggggagaaaaa.................................................................................................................................................................................................................................................................................... gtttttacctctttaataaatattattactcttcattttaccataaaatactctggaaataaagccaaaatagttattgggagttggtagagaagaaaaa.................................................................................................................................................................................................................................................................................... Subject: NC_003028_SP_0435_SP_0436|elongation factor P:aspartyl/glutamyl-tRNA amidotransferase subunit B|NEGATIVE:NEGATIVE|[408891,409018]|128 HSP 1 e-value: 8.0E-38 bit: 159.0 Len: 100 Query Start:1 Query End:100 Subject Strand: POSITIVE Subject Start: 1 Subject End: 100 gtttttacctctttaataaatattattactcttcattttaccataaaatcttctggaaataaagccaaaatagttattgggagatggtaggggagaaaaa.................................................................................................................................................................................................................................................................................... gtttttacctctttaataaatattattactcttcattttaccataaaatactctggaaataaagccaaaatagttattgggagttggtagagaagaaaaa.................................................................................................................................................................................................................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUUUUACCUCUUUAAUAAAUAUUAUUACUCUUCAUUUUACCAUAAAAUCUUCUGGAAAUAAAGCCAAAAUAGUUAUUGGGAGAUGGUAGGGGAGAAAAA .(((((.(((((.(((((.....)))))....(((((((.(((..........(((........)))..........)))))))))).))))).))))). (-15.25) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUUCUCCCCUACCAUCUCCCAAUAACUAUUUUGGCUUUAUUUCCAGAAGAUUUUAUGGUAAAAUGAAGAGUAAUAAUAUUUAUUAAAGAGGUAAAAAC ...........((((.(((...(((((.((((....(((((((((((..........)))..))))))))......)))).)))))..)))))))..... (-14.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 828 100 51 191 142 smit:SMT_319|5end_K02356_elongation_factor_EF-P_258864..259094_NEGATIVE 257 98 62 55 13 smit:SMT_2035|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1947167..1947397_POSITIVE 252 95 54 165 111 smit:SMT_1262|5end_ribosomal_protein_L11_methyltransferase_1227152..1227382_POSITIVE 237 98 54 211 157 smit:SMT_980|5end_hypothetical_protein_927440..927670_POSITIVE 230 98 54 71 16 smit:SMT_1366|5end_K02238_competence_protein_ComEC_1346888..1347118_NEGATIVE 229 97 52 211 168 smit:SMT_1807|5end_putative_mucus_binding_protein_1784043..1784273_NEGATIVE 228 96 56 125 79 smit:SMT_211|5end_hypothetical_protein_predicted_by_Glimmer/Critica_144135..144365_POSITIVE 217 48 1 153 92 smit:SMT_927|5end_KH_domain_protein_882677..882907_NEGATIVE 214 92 54 65 14 smit:SMT_518|5end_biotin_synthase_443751..443981_NEGATIVE 213 92 51 57 20 smit:SMT_1128|5end_surface_antigen_1063140..1063370_NEGATIVE 209 97 55 224 189 smit:SMT_1930|5end_peptide_chain_release_factor_1_1870765..1870995_NEGATIVE 209 65 26 151 106 smit:SMT_1702|5end_penicillin-binding_protein_2B_1684592..1684822_POSITIVE 204 96 64 41 9 smit:SMT_1489|5end_K06147_ATP-binding_cassette,_subfamily_B,_bacterial_1468892..1469122_POSITIVE 203 92 61 230 202 smit:SMT_1233|5end_cadmium_resistance_protein_1194306..1194536_POSITIVE 202 47 2 135 93 smit:SMT_2076|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1978592..1978822_NEGATIVE 201 93 54 192 158 smit:SMT_1356|5end_fibronectin-binding_protein-like_protein_A_1332534..1332764_POSITIVE 201 66 35 155 124 smit:SMT_1186|5end_hypothetical_protein_1144090..1144320_POSITIVE 201 67 41 42 15 smit:SMT_485|5end_hypothetical_protein_421401..421631_NEGATIVE 201 91 63 48 27 smit:SMT_214|5end_leucyl-tRNA_synthetase_146333..146563_POSITIVE 200 94 63 130 86 smit:SMT_821|5end_UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-_diaminopimelate_ligase_732775..733005_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 257 98 62 65 23 smit:SMT_2034|3end_hydrolase,_haloacid_dehalogenase-like_family_1947257..1947407_POSITIVE 254 97 52 58 9 smit:SMT_1911|3end_hypothetical_protein_1857840..1857990_POSITIVE 254 97 52 58 9 smit:SMT_867|3end_hypothetical_protein_816542..816692_POSITIVE 237 98 54 90 36 smit:SMT_979|3end_K03709_Mn-dependent_transcriptional_regulator_927399..927549_POSITIVE 230 98 54 87 32 smit:SMT_1367|3end_K02237_competence_protein_ComEA_1346872..1347022_NEGATIVE 227 97 52 81 38 smit:SMT_1581|3end_transcriptional_regulator,_biotin_repressor_family_1573120..1573270_NEGATIVE 217 91 53 72 23 smit:SMT_1070|3end_hypothetical_protein_1009644..1009794_NEGATIVE 216 94 53 55 13 smit:SMT_2109|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1999061..1999211_NEGATIVE 216 94 53 55 13 smit:SMT_1180|3end_putative_tagatose-6-phosphate_aldose/ketose_isomerase_1138036..1138186_NEGATIVE 210 94 55 70 34 smit:SMT_640|3end_PTS_system,_lactose-specific_IIBC_component_545099..545249_NEGATIVE 209 96 53 41 3 smit:SMT_965|3end_putative_transposase_911085..911235_NEGATIVE 208 92 55 90 49 smit:SMT_631|3end_glycerate_kinase_537307..537457_POSITIVE 204 96 64 51 19 smit:SMT_1488|3end_K06147_ATP-binding_cassette,_subfamily_B,_bacterial_1468982..1469132_POSITIVE 204 100 63 56 20 smit:SMT_1004|3end_thioredoxin_945824..945974_POSITIVE 199 98 63 58 19 smit:SMT_771|3end_spermidine/putrescine_transport_system_permease_protein_PotB_681356..681506_NEGATIVE 193 96 64 137 108 smit:SMT_2039|3end_malolactic_enzyme_1949681..1949831_NEGATIVE 193 94 63 70 39 smit:SMT_760|3end_K01990_ABC-2_type_transport_system_ATP-binding_protein_665693..665843_NEGATIVE 191 94 53 51 9 smit:SMT_1536|3end_trigger_factor_1520636..1520786_NEGATIVE 191 100 53 56 9 smit:SMT_1468|3end_pneumococcal_surface_protein,_putative_1448775..1448925_NEGATIVE 191 88 53 31 1 smit:SMT_627|3end_glucose-1-phosphate_adenylyltransferase_532921..533071_POSITIVE