Origin IGS: gcagaatataagatagaaggagtagggaaacccgctccctcttgtttaggcaatagtttgattgtcaaattatgacggtatcgaactattacgtaagcaagaaacgatggaggcact .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agtgcctccatcgtttcttgcttacgtaatagttcgataccgtcataatttgacaatcaaactattgcctaaacaagagggagcgggtttccctactccttctatcttatattctgc Mask Tandem Repeat Region ================================================ gcagaatataagatagaaggagtagggaaacccgctccctcttgtttaggcaatagtttgattgtcaaattatgacggtatcgaactattacgtaagcaagaaacgatggaggcact Find is-nt database================================================ Query_seq: SMT_307:SMT_308|SMT_307:SMT_308:K02078 acyl carrier protein:trans-2-enoyl-ACP reductase II:->->:249462..249578 117 gcagaatataagatagaaggagtagggaaacccgctccctcttgtttaggcaatagtttgattgtcaaattatgacggtatcgaactattacgtaagcaagaaacgatggaggcact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_307:SMT_308|SMT_307:SMT_308:K02078 acyl carrier protein:trans-2-enoyl-ACP reductase II:->->:249462..249578 117 gcagaatataagatagaaggagtagggaaacccgctccctcttgtttaggcaatagtttgattgtcaaattatgacggtatcgaactattacgtaagcaagaaacgatggaggcact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_307:SMT_308|SMT_307:SMT_308:K02078 acyl carrier protein:trans-2-enoyl-ACP reductase II:->->:249462..249578 117 gcagaatataagatagaaggagtagggaaacccgctccctcttgtttaggcaatagtttgattgtcaaattatgacggtatcgaactattacgtaagcaagaaacgatggaggcact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gcagaatataagatagaaggagtagggaaacccgctccctcttgtttaggcaatagtttgattgtcaaattatgacggtatcgaactattacgtaagcaagaaacgatggaggcact Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: - Start: 3 End: 116 .. S Y L I S P T P F G A G E Q K P L L K I T L N H R Y R V I V Y A L F S P P M . gcagaatataagatagaaggagtagggaaacccgctccctcttgtttaggcaatagtttgattgtcaaattatgacggtatcgaactattacgtaagcaagaaacgatggaggcact Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gcagaatataagatagaaggagtagggaaacccgctccctcttgtttaggcaatagtttgattgtcaaattatgacggtatcgaactattacgtaagcaagaaacgatggaggcact Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_307:SMT_308|SMT_307:SMT_308:K02078 acyl carrier protein:trans-2-enoyl-ACP reductase II:->->:249462..249578 117 gcagaatataagatagaaggagtagggaaacccgctccctcttgtttaggcaatagtttgattgtcaaattatgacggtatcgaactattacgtaagcaagaaacgatggaggcact Intra-Species Hit: Count: 1 Min: 1 Max: 117 Len: 117 Subject: smit_SMT_307_SMT_308|K02078 acyl carrier protein:trans-2-enoyl-ACP reductase II|POSITIVE:POSITIVE|[249462,249578]|117 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 117 Query Start:1 Query End:117 Subject Strand: POSITIVE Subject Start: 1 Subject End: 117 gcagaatataagatagaaggagtagggaaacccgctccctcttgtttaggcaatagtttgattgtcaaattatgacggtatcgaactattacgtaagcaagaaacgatggaggcact gcagaatataagatagaaggagtagggaaacccgctccctcttgtttaggcaatagtttgattgtcaaattatgacggtatcgaactattacgtaagcaagaaacgatggaggcact Inter-species Hit: Count: 3 Min: 1 Max: 117 Len: 117 Subject: NC_008533_SPD_0381_SPD_0382|acyl carrier protein:trans-2-enoyl-ACP reductase II|POSITIVE:POSITIVE|[385862,385979]|118 HSP 1 e-value: 5.0E-24 bit: 111.0 Len: 117 Query Start:1 Query End:117 Subject Strand: POSITIVE Subject Start: 1 Subject End: 118 gcagaatataagatagaaggagtaggga--aacccgctccctcttgtttaggcaatagtttgattgtcaaattatgacggtatcgaactattacgtaagcaagaaacgatggaggcact gcagaatattag-tagaaggagtagggagaaacccactccctcttgtttaggtaatagtttgactgtcaaattatggtgaaatcgaactattacgtaagcaaggaaggttggaggcact Subject: NC_003098_spr0378_spr0379|acyl carrier protein:Enoyl-acyl carrier protein(ACP) reductase.|POSITIVE:POSITIVE|[378333,378450]|118 HSP 1 e-value: 5.0E-24 bit: 111.0 Len: 117 Query Start:1 Query End:117 Subject Strand: POSITIVE Subject Start: 1 Subject End: 118 gcagaatataagatagaaggagtaggga--aacccgctccctcttgtttaggcaatagtttgattgtcaaattatgacggtatcgaactattacgtaagcaagaaacgatggaggcact gcagaatattag-tagaaggagtagggagaaacccactccctcttgtttaggtaatagtttgactgtcaaattatggtgaaatcgaactattacgtaagcaaggaaggttggaggcact Subject: NC_003028_SP_0418_SP_0419|acyl carrier protein:enoyl-(acyl-carrier-protein) reductase|POSITIVE:POSITIVE|[396917,397034]|118 HSP 1 e-value: 5.0E-24 bit: 111.0 Len: 117 Query Start:1 Query End:117 Subject Strand: POSITIVE Subject Start: 1 Subject End: 118 gcagaatataagatagaaggagtaggga--aacccgctccctcttgtttaggcaatagtttgattgtcaaattatgacggtatcgaactattacgtaagcaagaaacgatggaggcact gcagaatattag-tagaaggagtagggagaaacccactccctcttgtttaggtaatagtttgactgtcaaattatggtgaaatcgaactattacgtaagcaaggaaggttggaggcact Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCAGAAUAUAAGAUAGAAGGAGUAGGGAAACCCGCUCCCUCUUGUUUAGGCAAUAGUUUGAUUGUCAAAUUAUGACGGUAUCGAACUAUUACGUAAGCAAGAAACGAUGGAGGCACU ((................((((..((....))..)))).(((((((((.(.((((((((((((((((.....)))))..))))))))))).).)))))))))..........))... (-42.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUGCCUCCAUCGUUUCUUGCUUACGUAAUAGUUCGAUACCGUCAUAAUUUGACAAUCAAACUAUUGCCUAAACAAGAGGGAGCGGGUUUCCCUACUCCUUCUAUCUUAUAUUCUGC ...............(((((.(((.(((((((((.(((...((((.....)))).))).))))))))).))).))))).((((..((....))..)))).................. (-30.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 678 38 1 134 97 smit:SMT_1045|5end_cochaperonin_GroES_987582..987812_NEGATIVE 397 56 12 84 19 smit:SMT_929|5end_hypothetical_protein_883342..883572_NEGATIVE 397 56 12 208 143 smit:SMT_928|5end_hypothetical_protein_883218..883448_NEGATIVE 304 67 34 90 46 smit:SMT_994|5end_nicotinate-nucleotide_adenylyltransferase_936105..936335_NEGATIVE 291 73 31 51 3 smit:SMT_909|5end_type_I_restriction_enzyme_M_protein_858827..859057_NEGATIVE 284 63 24 179 143 smit:SMT_455|5end_cation_transport_ATPase_391670..391900_POSITIVE 281 43 20 72 53 smit:SMT_414|5end_K03665_GTP-binding_protein_HflX_357554..357784_POSITIVE 281 59 13 189 136 smit:SMT_92|5end_GTP-binding_protein,_probable_translation_factor_22857..23087_POSITIVE 272 78 31 229 178 smit:SMT_1749|5end_anthranilate_synthase_component_II_1727434..1727664_POSITIVE 267 79 31 86 30 smit:SMT_498|5end_hypothetical_protein_431751..431981_POSITIVE 264 53 14 81 28 smit:SMT_951|5end_oxidoreductase,_DadA_family_protein/D-amino_acid_oxidase_900098..900328_NEGATIVE 260 58 11 101 58 smit:SMT_226|5end_putative_L-glutamine-D-fructose-6-phosphate_amidotransferase_163630..163860_POSITIVE 259 67 30 171 138 smit:SMT_1696|5end_hypothetical_protein_1678182..1678412_POSITIVE 259 60 16 81 20 smit:SMT_1693|5end_dimethyladenosine_transferase_1675800..1676030_POSITIVE 259 67 23 159 112 smit:SMT_1634|5end_2,3,4,5-tetrahydropyridine-2-carboxylate_N-succinyltransferase,_putative_1621074..1621304_POSITIVE 255 60 11 203 152 smit:SMT_395|5end_hypothetical_protein_324459..324689_POSITIVE 250 44 9 145 112 smit:SMT_1616|5end_putative_ABC-type_cobalamin_Fe3+-siderophores_transport_system_ATPase_component_1603547..1603777_NEGATIVE 250 72 32 65 24 smit:SMT_743|5end_hypothetical_protein_648280..648510_NEGATIVE 246 43 9 151 106 smit:SMT_1307|5end_glycerol_uptake_facilitator_protein_1271664..1271894_POSITIVE 246 76 32 98 37 smit:SMT_945|5end_cytidylate_kinase_895908..896138_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 678 38 1 78 41 smit:SMT_1046|3end_chaperonin_GroEL_987638..987788_NEGATIVE 312 100 52 49 1 smit:SMT_307|3end_K02078_acyl_carrier_protein_249401..249551_POSITIVE 291 73 31 51 3 smit:SMT_910|3end_type_I_restriction_enzyme_R_protein_858827..858977_NEGATIVE 281 43 20 79 60 smit:SMT_413|3end_tRNA_delta(2)-isopentenylpyrophosphate_transferase_357641..357791_POSITIVE 281 59 13 104 51 smit:SMT_91|3end_hypothetical_protein_22852..23002_POSITIVE 267 79 31 65 9 smit:SMT_497|3end_hypothetical_protein_431810..431960_POSITIVE 264 53 14 64 11 smit:SMT_952|3end_hypothetical_protein_900115..900265_NEGATIVE 250 44 9 148 115 smit:SMT_1617|3end_K02015_iron_complex_transport_system_permease_protein_1603544..1603694_NEGATIVE 250 72 32 49 8 smit:SMT_744|3end_putative_glycosyltransferase_YibD_648296..648446_NEGATIVE 246 43 9 80 35 smit:SMT_1306|3end_alpha-glycerophosphate_oxidase_1271673..1271823_POSITIVE 246 76 32 88 27 smit:SMT_946|3end_hypothetical_protein_895918..896068_NEGATIVE 246 43 14 84 62 smit:SMT_804|3end_S-adenosylmethionine_synthetase_713884..714034_NEGATIVE 244 60 14 62 7 smit:SMT_1485|3end_exodeoxyribonuclease_1465169..1465319_NEGATIVE 243 66 21 69 21 smit:SMT_1948|3end_IS1381_transposase_protein_A_1883581..1883731_POSITIVE 242 54 11 80 21 smit:SMT_431|3end_UDP-N-acetylmuramoyl-L-alanyl-D-glutamate_synthetase_373631..373781_POSITIVE 241 66 25 63 17 smit:SMT_1946|3end_sucrose-6-phosphate_hydrolase_1881205..1881355_POSITIVE 241 50 10 71 35 smit:SMT_1278|3end_30S_ribosomal_protein_S2_1242332..1242482_POSITIVE 240 47 1 88 47 smit:SMT_1524|3end_peptidase_T_1505867..1506017_POSITIVE 240 67 35 38 1 smit:SMT_995|3end_K07574_putative_RNA-binding_protein_containing_KH_domain_936157..936307_NEGATIVE 233 62 30 138 105 smit:SMT_895|3end_cysteinyl-tRNA_synthetase_841694..841844_NEGATIVE