Origin IGS: gtaatcatgtggtgaacacattgttataaaaaaactatttattttaaaggagtcctatcatgacaatataagaaaaagtacaagaaattatcgttgaagaacttgggaaagacgcatcagaagtaacacttgaatcaacttttgatgat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atcatcaaaagttgattcaagtgttacttctgatgcgtctttcccaagttcttcaacgataatttcttgtactttttcttatattgtcatgataggactcctttaaaataaatagtttttttataacaatgtgttcaccacatgattac Mask Tandem Repeat Region ================================================ gtaatcatgtggtgaacacattgttataaaaaaactatttattttaaaggagtcctatcatgacaatataagaaaaagtacaagaaattatcgttgaagaacttgggaaagacgcatcagaagtaacacttgaatcaacttttgatgat Find is-nt database================================================ Query_seq: SMT_306:SMT_307|SMT_306:SMT_307:3-oxoacyl-:K02078 acyl carrier protein:->->:249178..249326 149 gtaatcatgtggtgaacacattgttataaaaaaactatttattttaaaggagtcctatcatgacaatataagaaaaagtacaagaaattatcgttgaagaacttgggaaagacgcatcagaagtaacacttgaatcaacttttgatgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_306:SMT_307|SMT_306:SMT_307:3-oxoacyl-:K02078 acyl carrier protein:->->:249178..249326 149 gtaatcatgtggtgaacacattgttataaaaaaactatttattttaaaggagtcctatcatgacaatataagaaaaagtacaagaaattatcgttgaagaacttgggaaagacgcatcagaagtaacacttgaatcaacttttgatgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_306:SMT_307|SMT_306:SMT_307:3-oxoacyl-:K02078 acyl carrier protein:->->:249178..249326 149 gtaatcatgtggtgaacacattgttataaaaaaactatttattttaaaggagtcctatcatgacaatataagaaaaagtacaagaaattatcgttgaagaacttgggaaagacgcatcagaagtaacacttgaatcaacttttgatgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gtaatcatgtggtgaacacattgttataaaaaaactatttattttaaaggagtcctatcatgacaatataagaaaaagtacaagaaattatcgttgaagaacttgggaaagacgcatcagaagtaacacttgaatcaacttttgatgat Predict ORF larger than 30AA ================================================ gtaatcatgtggtgaacacattgttataaaaaaactatttattttaaaggagtcctatcatgacaatataagaaaaagtacaagaaattatcgttgaagaacttgggaaagacgcatcagaagtaacacttgaatcaacttttgatgat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gtaatcatgtggtgaacacattgttataaaaaaactatttattttaaaggagtcctatcatgacaatataagaaaaagtacaagaaattatcgttgaagaacttgggaaagacgcatcagaagtaacacttgaatcaacttttgatgat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_306:SMT_307|SMT_306:SMT_307:3-oxoacyl-:K02078 acyl carrier protein:->->:249178..249326 149 gtaatcatgtggtgaacacattgttataaaaaaactatttattttaaaggagtcctatcatgacaatataagaaaaagtacaagaaattatcgttgaagaacttgggaaagacgcatcagaagtaacacttgaatcaacttttgatgat Intra-Species Hit: Count: 1 Min: 1 Max: 149 Len: 149 Subject: smit_SMT_306_SMT_307|3-oxoacyl-:K02078 acyl carrier protein|POSITIVE:POSITIVE|[249178,249326]|149 HSP 1 e-value: 7.0E-67 bit: 254.0 Len: 149 Query Start:1 Query End:149 Subject Strand: POSITIVE Subject Start: 1 Subject End: 149 gtaatcatgtggtgaacacattgttatnnnnnnnctatttattttaaaggagtcctatcatgacaatataagaaaaagtacaagaaattatcgttgaagaacttgggaaagacgcatcagaagtaacacttgaatcaacttttgatgat gtaatcatgtggtgaacacattgttataaaaaaactatttattttaaaggagtcctatcatgacaatataagaaaaagtacaagaaattatcgttgaagaacttgggaaagacgcatcagaagtaacacttgaatcaacttttgatgat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUAAUCAUGUGGUGAACACAUUGUUAUAAAAAAACUAUUUAUUUUAAAGGAGUCCUAUCAUGACAAUAUAAGAAAAAGUACAAGAAAUUAUCGUUGAAGAACUUGGGAAAGACGCAUCAGAAGUAACACUUGAAUCAACUUUUGAUGAU (((((.(((((.....))))).))))).........................(((((......((((((((................)))).)))).......)))))......((((((((((.............)))))))))).. (-22.63) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCAUCAAAAGUUGAUUCAAGUGUUACUUCUGAUGCGUCUUUCCCAAGUUCUUCAACGAUAAUUUCUUGUACUUUUUCUUAUAUUGUCAUGAUAGGACUCCUUUAAAAUAAAUAGUUUUUUUAUAACAAUGUGUUCACCACAUGAUUAC ..(((((.((((.(((......))))))).)))))..........((((....(((.((.....))))).)))).....((((((((.((((.((((((..((......))..)))))).)))).))))))))................ (-19.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 235 122 93 214 176 smit:SMT_1617|5end_K02015_iron_complex_transport_system_permease_protein_1604500..1604730_NEGATIVE 235 125 90 215 183 smit:SMT_345|5end_K01990_ABC-2_type_transport_system_ATP-binding_protein_283992..284222_POSITIVE 222 107 81 206 183 smit:SMT_1669|5end_potassium_uptake_protein_KtrB_1658577..1658807_NEGATIVE 222 68 48 47 23 smit:SMT_81|5end_K01421_putative_membrane_protein_13630..13860_NEGATIVE 219 139 102 112 70 smit:SMT_777|5end_hypothetical_protein_686770..687000_NEGATIVE 216 70 35 216 182 smit:SMT_1442|5end_hypothetical_protein_1426328..1426558_NEGATIVE 212 137 92 110 55 smit:SMT_519|5end_glutathione_reductase_444884..445114_NEGATIVE 210 132 91 173 114 smit:SMT_428|5end_ribosomal_small_subunit_pseudouridine_synthase_A_369857..370087_NEGATIVE 209 138 93 55 3 smit:SMT_836|5end_cell_surface_protein_precursor_779832..780062_NEGATIVE 208 137 103 42 3 smit:SMT_1551|5end_K02221_YggT_family_protein_1534859..1535089_POSITIVE 207 117 75 91 54 smit:SMT_1040|5end_K03565_regulatory_protein_982602..982832_POSITIVE 204 132 91 55 13 smit:SMT_1265|5end_hypothetical_protein_1231261..1231491_NEGATIVE 203 134 92 60 20 smit:SMT_880|5end_beta-lactam_resistance_factor_828300..828530_NEGATIVE 200 140 96 104 63 smit:SMT_1835|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1803894..1804124_POSITIVE 200 132 101 140 109 smit:SMT_1729|5end_exodeoxyribonuclease_VII_small_subunit_1714566..1714796_POSITIVE 200 127 81 173 131 smit:SMT_425|5end_hypothetical_cytosolic_protein_368114..368344_POSITIVE 199 137 103 48 4 smit:SMT_1307|5end_glycerol_uptake_facilitator_protein_1271664..1271894_POSITIVE 199 131 98 228 188 smit:SMT_650|5end_pullulanase_559832..560062_NEGATIVE 196 140 91 221 164 smit:SMT_1093|5end_K03073_preprotein_translocase_SecE_subunit_1033926..1034156_NEGATIVE 195 117 71 153 116 smit:SMT_285|5end_polysaccharide_polymerase_227866..228096_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 242 119 77 65 20 smit:SMT_657|3end_Kef-type_K+_transport_system,_predicted_NAD-binding_component_568427..568577_NEGATIVE 234 128 83 136 93 smit:SMT_802|3end_aldo/keto_reductase_family_711486..711636_NEGATIVE 216 70 35 41 7 smit:SMT_1443|3end_23S_rRNA_m(5)U_1939_methyltransferase_1426503..1426653_NEGATIVE 212 137 92 113 58 smit:SMT_520|3end_glutathione_reductase_444881..445031_NEGATIVE 211 69 52 149 132 smit:SMT_91|3end_hypothetical_protein_22852..23002_POSITIVE 208 137 103 42 3 smit:SMT_1550|3end_hypothetical_protein_1534939..1535089_POSITIVE 203 140 106 36 1 smit:SMT_1449|3end_K08591_putative_membrane_protein_1432658..1432808_POSITIVE 203 134 92 55 15 smit:SMT_881|3end_peptidoglycan_branched_peptide_synthesis_protein,_alanine_adding_enzyme_828305..828455_NEGATIVE 200 115 87 134 103 smit:SMT_1624|3end_formate_acetyltransferase_1611421..1611571_NEGATIVE 200 127 81 87 45 smit:SMT_424|3end_transcriptional_regulatory_protein_368108..368258_POSITIVE 199 131 98 74 34 smit:SMT_651|3end_DNA_ligase_559986..560136_NEGATIVE 188 117 93 112 79 smit:SMT_1156|3end_K07485_transposase_1100158..1100308_POSITIVE 187 139 91 87 45 smit:SMT_386|3end_branched-chain_amino_acid_transport_system_II_carrier_protein_321002..321152_POSITIVE 184 115 81 82 45 smit:SMT_833|3end_hypothetical_protein_predicted_by_Glimmer/Critica_775183..775333_NEGATIVE 182 126 89 48 3 smit:SMT_934|3end_pyridine_nucleotide-disulfide_oxidoreductase_887307..887457_POSITIVE 180 133 93 54 11 smit:SMT_1977|3end_endopeptidase_O_1908454..1908604_POSITIVE 179 140 92 109 49 smit:SMT_891|3end_oxidoreductase,_putative_839132..839282_POSITIVE 176 124 92 64 31 smit:SMT_2163|3end_cell_division_protein_FtsA_2037917..2038067_POSITIVE 176 107 77 81 50 smit:SMT_1420|3end_K02004_1404594..1404744_NEGATIVE 176 80 33 72 8 smit:SMT_598|3end_K07273_lysozyme_514012..514162_NEGATIVE