Origin IGS: ttaattaannnnngccagtccagtgtgctggaaagcttattagcgtcaggagttctagctgcgtgttcaacactagttcaaataatgctamactgaaacgagaagtacccgcagtgaagtcaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 attgacttcactgcgggtacttctcgtttcagtktagcattatttgaactagtgttgaacacgcagctagaactcctgacgctaataagctttccagcacactggactggcnnnnnttaattaa Mask Tandem Repeat Region ================================================ ttaattaannnnngccagtccagtgtgctggaaagcttattagcgtcaggagttctagctgcgtgttcaacactagttcaaataatgctamactgaaacgagaagtacccgcagtgaagtcaat Find is-nt database================================================ Query_seq: SMT_302:SMT_303|SMT_302:SMT_303:K07444 putative N6-adenine-specific DNA methylase:hypothetical protein:->->:245380..245503 124 ttaattaannnnngccagtccagtgtgctggaaagcttattagcgtcaggagttctagctgcgtgttcaacactagttcaaataatgctamactgaaacgagaagtacccgcagtgaagtcaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_302:SMT_303|SMT_302:SMT_303:K07444 putative N6-adenine-specific DNA methylase:hypothetical protein:->->:245380..245503 124 ttaattaannnnngccagtccagtgtgctggaaagcttattagcgtcaggagttctagctgcgtgttcaacactagttcaaataatgctamactgaaacgagaagtacccgcagtgaagtcaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_302:SMT_303|SMT_302:SMT_303:K07444 putative N6-adenine-specific DNA methylase:hypothetical protein:->->:245380..245503 124 ttaattaannnnngccagtccagtgtgctggaaagcttattagcgtcaggagttctagctgcgtgttcaacactagttcaaataatgctamactgaaacgagaagtacccgcagtgaagtcaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttaattaannnnngccagtccagtgtgctggaaagcttattagcgtcaggagttctagctgcgtgttcaacactagttcaaataatgctamactgaaacgagaagtacccgcagtgaagtcaat Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 4 End: 93 ... M X X X Q S S V L E S L L A S G V L A A C S T L V Q I M L X ............................... Protein_Len: 39 Strand: - Start: 8 End: 124 ....... X X G T W H A P F S I L T L L E L Q T N L V L E F L A X S F R S T G A T F D M ttaattaannnnngccagtccagtgtgctggaaagcttattagcgtcaggagttctagctgcgtgttcaacactagttcaaataatgctamactgaaacgagaagtacccgcagtgaagtcaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttaattaannnnngccagtccagtgtgctggaaagcttattagcgtcaggagttctagctgcgtgttcaacactagttcaaataatgctamactgaaacgagaagtacccgcagtgaagtcaat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_302:SMT_303|SMT_302:SMT_303:K07444 putative N6-adenine-specific DNA methylase:hypothetical protein:->->:245380..245503 124 ttaattaannnnngccagtccagtgtgctggaaagcttattagcgtcaggagttctagctgcgtgttcaacactagttcaaataatgctamactgaaacgagaagtacccgcagtgaagtcaat Intra-Species Hit: Count: 1 Min: 14 Max: 124 Len: 111 Subject: smit_SMT_302_SMT_303|K07444 putative N6-adenine-specific DNA methylase:hypothetical protein|POSITIVE:POSITIVE|[245380,245503]|124 HSP 1 e-value: 5.0E-56 bit: 217.0 Len: 111 Query Start:14 Query End:124 Subject Strand: POSITIVE Subject Start: 14 Subject End: 124 .............gccagtccagtgtgctggaaagcttattagcgtcaggagttctagctgcgtgttcaacactagttcaaataatgctamactgaaacgagaagtacccgcagtgaagtcaat .............gccagtccagtgtgctggaaagcttattagcgtcaggagttctagctgcgtgttcaacactagttcaaataatgctamactgaaacgagaagtacccgcagtgaagtcaat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCCAGUCCAGUGUGCUGGAAAGCUUAUUAGCGUCAGGAGUUCUAGCUGCGUGUUCAACACUAGUUCAAAUAAUGCUAMACUGAAACGAGAAGUACCCGCAGUGAAGUCAAU .....(((((....)))))..(((....))).....((.(((..((((((.((......((..((((.............))))...))....)).))))))))).))... (-24.52) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUGACUUCACUGCGGGUACUUCUCGUUUCAGUKUAGCAUUAUUUGAACUAGUGUUGAACACGCAGCUAGAACUCCUGACGCUAAUAAGCUUUCCAGCACACUGGACUGGC ............(((((.....)))))....((.((((((((.......)))))))).)).....(((((..........(((....)))..(((((....)))))))))) (-22.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 505 40 1 211 173 smit:SMT_543|5end_topoisomerase_IV_subunit_B_458372..458602_POSITIVE 491 46 1 59 13 smit:SMT_956|5end_hypothetical_protein_predicted_by_Glimmer/Critica_904789..905019_NEGATIVE 485 48 1 62 7 smit:SMT_1957|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1890645..1890875_POSITIVE 454 49 1 191 128 smit:SMT_1983|5end_glycerophosphoryl_diester_phosphodiesterase_1913691..1913921_NEGATIVE 422 37 1 229 193 smit:SMT_389|5end_PTS_system,_galactitol-specific_IIA_component,_putative_321779..322009_POSITIVE 398 50 1 107 55 smit:SMT_1942|5end_zinc_metalloprotease_ZmpB,_truncated_1877882..1878112_POSITIVE 374 47 1 167 115 smit:SMT_367|5end_hypothetical_protein_302318..302548_POSITIVE 336 48 1 78 30 smit:SMT_807|5end_hypothetical_protein_predicted_by_Glimmer/Critica_716815..717045_NEGATIVE 330 49 1 229 186 smit:SMT_2051|5end_enoyl-CoA_hydratase_1960639..1960869_NEGATIVE 310 48 7 96 51 smit:SMT_1967|5end_dicarboxylate/amino_acid:cation_(Na+_or_H+)_symporter_(DAACS)_family_protein_1899683..1899913_NEGATIVE 308 50 2 204 156 smit:SMT_949|5end_glycosyl_transferase,_family_2_897693..897923_NEGATIVE 298 50 10 151 108 smit:SMT_1596|5end_oligopeptide_transport_ATP-binding_protein_OppD_1585903..1586133_POSITIVE 296 47 1 196 159 smit:SMT_1671|5end_hypothetical_protein_1659757..1659987_NEGATIVE 295 49 3 227 190 smit:SMT_225|5end_pullulanase_163529..163759_NEGATIVE 291 16 1 231 216 smit:SMT_1872|5end_K03217_preprotein_translocase_YidC_subunit_1831764..1831994_NEGATIVE 283 49 1 209 146 smit:SMT_1377|5end_hypothetical_protein_1355873..1356103_NEGATIVE 283 57 27 222 186 smit:SMT_984|5end_transcriptional_regulator_Spx_930898..931128_NEGATIVE 281 45 1 220 155 smit:SMT_1370|5end_K06217_phosphate_starvation-inducible_protein_PhoH_and_related_proteins_1350494..1350724_NEGATIVE 277 76 34 183 137 smit:SMT_1816|5end_K02026_multiple_sugar_transport_system_permease_protein_1790813..1791043_POSITIVE 277 50 2 47 4 smit:SMT_1719|5end_DNA_mismatch_repair_protein_1706842..1707072_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 519 44 1 149 111 smit:SMT_1886|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1841198..1841348_POSITIVE 500 31 1 151 121 smit:SMT_542|3end_hypothetical_protein_458400..458550_POSITIVE 491 46 1 72 26 smit:SMT_957|3end_UDP-glucose_4-epimerase_904776..904926_NEGATIVE 472 49 1 135 67 smit:SMT_1631|3end_phosphopantothenoylcysteine_synthase/decarboxylase_1618204..1618354_NEGATIVE 471 32 1 151 121 smit:SMT_1985|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1914407..1914557_NEGATIVE 469 45 1 149 111 smit:SMT_1658|3end_hypothetical_protein_1648937..1649087_POSITIVE 468 35 1 145 111 smit:SMT_956|3end_hypothetical_protein_predicted_by_Glimmer/Critica_904691..904841_NEGATIVE 463 47 1 145 111 smit:SMT_2044|3end_transcriptional_activator_TipA,_putative_1955274..1955424_NEGATIVE 435 31 1 142 111 smit:SMT_2016|3end_tyrosine_recombinase_1933021..1933171_NEGATIVE 431 25 1 139 114 smit:SMT_1984|3end_phosphinothricin_N-acetyltransferase_1913705..1913855_NEGATIVE 428 37 1 109 69 smit:SMT_1955|3end_carbamoyl-phosphate_synthase_small_chain_1888992..1889142_NEGATIVE 424 34 1 144 111 smit:SMT_1558|3end_hypothetical_protein_1543855..1544005_NEGATIVE 422 24 1 145 121 smit:SMT_1954|3end_6-phospho-beta-glucosidase_1888831..1888981_POSITIVE 422 41 1 146 111 smit:SMT_1155|3end_ABC-type_oligopeptide_transport_system,_periplasmic_component_1099183..1099333_NEGATIVE 420 26 1 140 111 smit:SMT_1957|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1890829..1890979_POSITIVE 418 32 1 148 111 smit:SMT_2026|3end_K03466_DNA_segregation_ATPase_FtsK/SpoIIIE,_S-DNA-T_family_1941905..1942055_NEGATIVE 417 28 1 136 111 smit:SMT_2006|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1926803..1926953_POSITIVE 415 24 1 146 121 smit:SMT_1464|3end_K02030_polar_amino_acid_transport_system_substrate-binding_protein_1445361..1445511_POSITIVE 414 22 1 142 121 smit:SMT_2001|3end_membrane_protease_protein_family_1923889..1924039_POSITIVE 383 47 1 86 34 smit:SMT_1895|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1847402..1847552_POSITIVE