Origin IGS: gagattatttgaaaagtgcaatttttggataatcgcgtgaggagaatttcttttcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagagatcgttacggcagttgaaatggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatggagtgcacggaaacgaacgtagtactctgactgctatcagctataggctgttagtggtagacagatgattatcaaaggaagtggtcctagtcacttctggaacaaaacatggcttatagaaaattgcatataggttggggctgagaaattttctcaacctcattttttaaagtggacatatagaaaggtcttataagactgaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttcagtcttataagacctttctatatgtccactttaaaaaatgaggttgagaaaatttctcagccccaacctatatgcaattttctataagccatgttttgttccagaagtgactaggaccacttcctttgataatcatctgtctaccactaacagcctatagctgatagcagtcagagtactacgttcgtttccgtgcactccatgccccgaccgaaatttgggttgctagcttgaggggtttaccgcgttccactctctccgtttccagaaagactccgtcactgtggcactttcaagcctactctgacctatccgaagacttagccatttcaactgccgtaacgatctctcgtccctaggcttatggtttcgcctagcacaaacactacaggcatcacagcctgtgctagcatggactttcctcatgaaaagaaattctcctcacgcgattatccaaaaattgcacttttcaaataatctc Mask Tandem Repeat Region ================================================ gagattatttgaaaagtgcaatttttggataatcgcgtgaggagaatttcttttcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagagatcgttacggcagttgaaatggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatggagtgcacggaaacgaacgtagtactctgactgctatcagctataggctgttagtggtagacagatgattatcaaaggaagtggtcctagtcacttctggaacaaaacatggcttatagaaaattgcatataggttggggctgagaaattttctcaacctcattttttaaagtggacatatagaaaggtcttataagactgaaa Find is-nt database================================================ Query_seq: SMT_301:SMT_302|SMT_301:SMT_302:hypothetical protein:K07444 putative N6-adenine-specific DNA methylase:->->:244463..244947 485 gagattatttgaaaagtgcaatttttggataatcgcgtgaggagaatttcttttcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagagatcgttacggcagttgaaatggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatggagtgcacggaaacgaacgtagtactctgactgctatcagctataggctgttagtggtagacagatgattatcaaaggaagtggtcctagtcacttctggaacaaaacatggcttatagaaaattgcatataggttggggctgagaaattttctcaacctcattttttaaagtggacatatagaaaggtcttataagactgaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_301:SMT_302|SMT_301:SMT_302:hypothetical protein:K07444 putative N6-adenine-specific DNA methylase:->->:244463..244947 485 gagattatttgaaaagtgcaatttttggataatcgcgtgaggagaatttcttttcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagagatcgttacggcagttgaaatggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatggagtgcacggaaacgaacgtagtactctgactgctatcagctataggctgttagtggtagacagatgattatcaaaggaagtggtcctagtcacttctggaacaaaacatggcttatagaaaattgcatataggttggggctgagaaattttctcaacctcattttttaaagtggacatatagaaaggtcttataagactgaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_301:SMT_302|SMT_301:SMT_302:hypothetical protein:K07444 putative N6-adenine-specific DNA methylase:->->:244463..244947 485 gagattatttgaaaagtgcaatttttggataatcgcgtgaggagaatttcttttcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagagatcgttacggcagttgaaatggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatggagtgcacggaaacgaacgtagtactctgactgctatcagctataggctgttagtggtagacagatgattatcaaaggaagtggtcctagtcacttctggaacaaaacatggcttatagaaaattgcatataggttggggctgagaaattttctcaacctcattttttaaagtggacatatagaaaggtcttataagactgaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gagattatttgaaaagtgcaatttttggataatcgcgtgaggagaatttcttttcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagagatcgttacggcagttgaaatggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatggagtgcacggaaacgaacgtagtactctgactgctatcagctataggctgttagtggtagacagatgattatcaaaggaagtggtcctagtcacttctggaacaaaacatggcttatagaaaattgcatataggttggggctgagaaattttctcaacctcattttttaaagtggacatatagaaaggtcttataagactgaaa Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 56 End: 253 ....................................................... M R K V H A S T G C D A C S V C A R R N H K P R D E R S L R Q L K W L S L R I G Q S R L E S A T V T E S F W K R R E W N ........................................................................................................................................................................................................................................ Protein_Len: 40 Strand: - Start: 325 End: 444 .................................................................................................................................................................................................................................................................................................................................... L S N T T S L H N D F S T T R T V E P V F C P K Y F I A Y L N P S L F K R L R M ......................................... Protein_Len: 47 Strand: - Start: 126 End: 266 ............................................................................................................................. P R S I T V A T S I A L D E S L D S Y A Q F H W L S P T K R S V S L T S R P L G R L S A V W M ........................................................................................................................................................................................................................... Protein_Len: 37 Strand: - Start: 8 End: 118 ....... K F L A I K P Y D R S S F K K E H P F D M S A C A T I G T T N T S P S V M ............................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 57 Strand: - Start: 1 End: 171 L N N S F H L K Q I I A H P S N R K M L F T W A L V P Q S A Q L T Q A L R F W L G L S S L D N R C N F H S L R R M .......................................................................................................................................................................................................................................................................................................................... gagattatttgaaaagtgcaatttttggataatcgcgtgaggagaatttcttttcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagagatcgttacggcagttgaaatggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatggagtgcacggaaacgaacgtagtactctgactgctatcagctataggctgttagtggtagacagatgattatcaaaggaagtggtcctagtcacttctggaacaaaacatggcttatagaaaattgcatataggttggggctgagaaattttctcaacctcattttttaaagtggacatatagaaaggtcttataagactgaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gagattatttgaaaagtgcaatttttggataatcgcgtgaggagaatttcttttcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagagatcgttacggcagttgaaatggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatggagtgcacggaaacgaacgtagtactctgactgctatcagctataggctgttagtggtagacagatgattatcaaaggaagtggtcctagtcacttctggaacaaaacatggcttatagaaaattgcatataggttggggctgagaaattttctcaacctcattttttaaagtggacatatagaaaggtcttataagactgaaa Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 51 HP_score: -3.3 Tail_Score: -3.57406 Start: 76 End: 96 Full_Region: GAAAGTCCATGCTAG CACAGGC TGTGAT GCCTGTAG TGTTTGTGCTAGGCG ...........................................................................CACAGGCTGTGATGCCTGTAG..................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 44 HP_score: -3.6 Tail_Score: -2.8829 Start: 359 End: 378 Full_Region: ccatgttttgttcca gaagtg actaggac cacttc ctttgataatcatct ......................................................................................................................................................................................................................................................................................................................................................................gaagtgactaggaccacttc........................................................................................................... TransTerm Strand: + Conf: 93 HP_score: -10.2 Tail_Score: -5.0538 Start: 417 End: 443 Full_Region: AAATTGCATATAGGT TGGGGCTGAGAA ATT TTCTCAACCTCA TTTTTTAAAGTGGAC ................................................................................................................................................................................................................................................................................................................................................................................................................................TGGGGCTGAGAAATTTTCTCAACCTCA.......................................... Find igs database================================================ Query_seq: SMT_301:SMT_302|SMT_301:SMT_302:hypothetical protein:K07444 putative N6-adenine-specific DNA methylase:->->:244463..244947 485 gagattatttgaaaagtgcaatttttggataatcgcgtgaggagaatttcttttcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagagatcgttacggcagttgaaatggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatggagtgcacggaaacgaacgtagtactctgactgctatcagctataggctgttagtggtagacagatgattatcaaaggaagtggtcctagtcacttctggaacaaaacatggcttatagaaaattgcatataggttggggctgagaaattttctcaacctcattttttaaagtggacatatagaaaggtcttataagactgaaa Intra-Species Hit: Count: 1 Min: 1 Max: 485 Len: 485 Subject: smit_SMT_301_SMT_302|hypothetical protein:K07444 putative N6-adenine-specific DNA methylase|POSITIVE:POSITIVE|[244463,244947]|485 HSP 1 e-value: 0.0 bit: 961.0 Len: 485 Query Start:1 Query End:485 Subject Strand: POSITIVE Subject Start: 1 Subject End: 485 gagattatttgaaaagtgcaatttttggataatcgcgtgaggagaatttcttttcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagagatcgttacggcagttgaaatggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatggagtgcacggaaacgaacgtagtactctgactgctatcagctataggctgttagtggtagacagatgattatcaaaggaagtggtcctagtcacttctggaacaaaacatggcttatagaaaattgcatataggttggggctgagaaattttctcaacctcattttttaaagtggacatatagaaaggtcttataagactgaaa gagattatttgaaaagtgcaatttttggataatcgcgtgaggagaatttcttttcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagagatcgttacggcagttgaaatggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatggagtgcacggaaacgaacgtagtactctgactgctatcagctataggctgttagtggtagacagatgattatcaaaggaagtggtcctagtcacttctggaacaaaacatggcttatagaaaattgcatataggttggggctgagaaattttctcaacctcattttttaaagtggacatatagaaaggtcttataagactgaaa Inter-species Hit: Count: 25 Min: 5 Max: 482 Len: 478 Subject: NC_008533_SPD_0339_SPD_0341|hypothetical protein:RNA methylase family protein UPF0020, putative|POSITIVE:POSITIVE|[344137,344621]|485 HSP 1 e-value: 0.0 bit: 797.0 Len: 478 Query Start:5 Query End:482 Subject Strand: POSITIVE Subject Start: 5 Subject End: 481 ....ttatttgaaaagtgcaatttttggataatcgcgtgaggagaatttcttttcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagagatcgttacggcagttgaaatggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatggagtgcacggaaacgaacgtagtactctgactgctatcagctataggctgttagtggtagacagatgattatcaaaggaagtggtcctagtcacttctggaacaaaacatggcttatagaaaattgcatataggttggggctgagaaattttctcaacctcattttttaaagtggacatatagaaaggtcttataagactg... ....ttatttgagatgtgcaatttttggataatcgcgtgaggagaattgcttctcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagaaatcgttacggcagttgaaatggctaagtccttggataggccagagtaggcttgaaagtgccacagtgacggagtctttctggaaacagagagagtggaacgcggtaaacccctcaagctagcaacccaaattttggtcggggcatggagtacgcggaaacgaacgtagtattctgactgctatcagctaga-gctgttagtggtagacagatgattatcgaaggaagtggtcctagtcacttctggaacaaaacatggcttatagaaaattgcatataggttggggctgagaaattttctcaacctcattttttaaagtggacatatagaaaggtcttgcaagactg... Subject: NC_003098_spr0332_spr0333|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[336608,337077]|470 HSP 1 e-value: 0.0 bit: 791.0 Len: 467 Query Start:5 Query End:471 Subject Strand: POSITIVE Subject Start: 5 Subject End: 470 ....ttatttgaaaagtgcaatttttggataatcgcgtgaggagaatttcttttcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagagatcgttacggcagttgaaatggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatggagtgcacggaaacgaacgtagtactctgactgctatcagctataggctgttagtggtagacagatgattatcaaaggaagtggtcctagtcacttctggaacaaaacatggcttatagaaaattgcatataggttggggctgagaaattttctcaacctcattttttaaagtggacatatagaaaggtc.............. ....ttatttgagatgtgcaatttttggataatcgcgtgaggagaattgcttctcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagaaatcgttacggcagttgaaatggctaagtccttggataggccagagtaggcttgaaagtgccacagtgacggagtctttctggaaacagagagagtggaacgcggtaaacccctcaagctagcaacccaaattttggtcggggcatggagtacgcggaaacgaacgtagtattctgactgctatcagctaga-gctgttagtggtagacagatgattatcgaaggaagtggtcctagtcacttctggaacaaaacatggcttatagaaaattgcatataggttggggctgagaaattttctcaacctcattttttaaagtggacatatagaaaggtc.............. Subject: NC_003028_SP_0372_SP_0373|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[350598,351082]|485 HSP 1 e-value: 0.0 bit: 789.0 Len: 478 Query Start:5 Query End:482 Subject Strand: POSITIVE Subject Start: 5 Subject End: 481 ....ttatttgaaaagtgcaatttttggataatcgcgtgaggagaatttcttttcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagagatcgttacggcagttgaaatggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatggagtgcacggaaacgaacgtagtactctgactgctatcagctataggctgttagtggtagacagatgattatcaaaggaagtggtcctagtcacttctggaacaaaacatggcttatagaaaattgcatataggttggggctgagaaattttctcaacctcattttttaaagtggacatatagaaaggtcttataagactg... ....ttatttgagatgtgcaatttttggataatcgcgtgaggagaattgtttctcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagaaatcgttacggcagttgaaatggctaagtccttggataggccagagtaggcttgaaagtgccacagtgacggagtctttctggaaacagagagagtggaacgcggtaaacccctcaagctagcaacccaaattttggtcggggcatggagtacgcggaaacgaacgtagtattctgactgctatcagctaga-gctgttagtggtagacagatgattatcgaaggaagtggtcctagtcacttctggaacaaaacatggcttatagaaaattgcatataggttggggctgagaaattttctcaacctcattttttaaagtggacatatagaaaggtcttgcaagactg... Subject: NC_009009_SSA_1856_SSA_1857|hypothetical protein:Conserved DivIVA-like protein, putative|NEGATIVE:NEGATIVE|[1852299,1852814]|516 HSP 1 e-value: 4.0E-62 bit: 240.0 Len: 300 Query Start:15 Query End:314 Subject Strand: NEGATIVE Subject Start: 200 Subject End: 501 ..............agtgcaatttttggataatcgcgtgaggagaatttc--ttttcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggacgagagat---cgttacggcagttgaaatggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatggagtgcacggaaacgaacgtagtactctgactg........................................................................................................................................................................... ..............agtgcaatttttggataatcgcgtg--gaaattttagattttcatgaggaaagtccatgctagcactggctgtgatgccggtagtgtttgtgctaggcgaagaaataagcctagggacg-gatttttccgttacggcgactgaaagggctaagtctttggatatgtctgattaggtctgaaagtgccacagtgacgtagttcttttggaaacagaagaagtggaacgcggtaaacccctcaagctagcaacccaaactttggtcggggcatggagtgcgtggaagcgaacatagcactctgactg........................................................................................................................................................................... HSP 2 e-value: 2.0E-20 bit: 101.0 Len: 78 Query Start:338 Query End:415 Subject Strand: NEGATIVE Subject Start: 115 Subject End: 193 .................................................................................................................................................................................................................................................................................................................................................gtagacagatgattatcaaaggaagtggt-cctagtcacttctggaacaaaacatggcttatagaaaattgcatatagg...................................................................... .................................................................................................................................................................................................................................................................................................................................................gtagacagatgattatcgaaggaaatggtacctagtcatttccggaacaaaacatggcttacagaaaattgcatatagg...................................................................... Subject: NC_006449_str0233_str0234|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[237446,237971]|526 HSP 1 e-value: 2.0E-30 bit: 135.0 Len: 164 Query Start:155 Query End:318 Subject Strand: POSITIVE Subject Start: 151 Subject End: 314 ..........................................................................................................................................................tggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatggagtgcacggaaacgaacgtagtactctgactgctat....................................................................................................................................................................... ..........................................................................................................................................................tggctaagtcttcggataggttttagtagctctgaaagtgccacagtgacggagtttttagggaaacctaaaaagtggaacgcggtaaacccctcaagctagcaacccaaactttggtaggggcatgggatggccggaaacgaacggaccattctgactgctat....................................................................................................................................................................... HSP 2 e-value: 4.0E-28 bit: 127.0 Len: 79 Query Start:338 Query End:416 Subject Strand: POSITIVE Subject Start: 318 Subject End: 397 .................................................................................................................................................................................................................................................................................................................................................gtagacagatgattatcaaaggaagtggt-cctagtcacttctggaacaaaacatggcttatagaaaattgcatataggt..................................................................... .................................................................................................................................................................................................................................................................................................................................................gtagacagatgattatcgaaggaagtggtacctagtcacttctggaacagaacatggcttatagaaaattgcatataggt..................................................................... HSP 3 e-value: 1.0E-25 bit: 119.0 Len: 76 Query Start:55 Query End:130 Subject Strand: POSITIVE Subject Start: 48 Subject End: 123 ......................................................catgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggac................................................................................................................................................................................................................................................................................................................................................................... ......................................................catgaggaaagtccatgctagcactggctgtgatgccagtagtgtttgtgctaggcgaatcaataagcctagggac................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_006448_stu0233_stu0234|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[237407,237932]|526 HSP 1 e-value: 2.0E-30 bit: 135.0 Len: 164 Query Start:155 Query End:318 Subject Strand: POSITIVE Subject Start: 151 Subject End: 314 ..........................................................................................................................................................tggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatggagtgcacggaaacgaacgtagtactctgactgctat....................................................................................................................................................................... ..........................................................................................................................................................tggctaagtcttcggataggttttagtagctctgaaagtgccacagtgacggagtttttagggaaacctaaaaagtggaacgcggtaaacccctcaagctagcaacccaaactttggtaggggcatgggatggccggaaacgaacggaccattctgactgctat....................................................................................................................................................................... HSP 2 e-value: 4.0E-28 bit: 127.0 Len: 79 Query Start:338 Query End:416 Subject Strand: POSITIVE Subject Start: 318 Subject End: 397 .................................................................................................................................................................................................................................................................................................................................................gtagacagatgattatcaaaggaagtggt-cctagtcacttctggaacaaaacatggcttatagaaaattgcatataggt..................................................................... .................................................................................................................................................................................................................................................................................................................................................gtagacagatgattatcgaaggaagtggtacctagtcacttctggaacagaacatggcttatagaaaattgcatataggt..................................................................... HSP 3 e-value: 1.0E-25 bit: 119.0 Len: 76 Query Start:55 Query End:130 Subject Strand: POSITIVE Subject Start: 48 Subject End: 123 ......................................................catgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctagggac................................................................................................................................................................................................................................................................................................................................................................... ......................................................catgaggaaagtccatgctagcactggctgtgatgccagtagtgtttgtgctaggcgaatcaataagcctagggac................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_007432_SAK_0373_SAK_0374|DivIVA domain protein:hypothetical protein|POSITIVE:POSITIVE|[321731,322199]|469 HSP 1 e-value: 1.0E-24 bit: 115.0 Len: 74 Query Start:56 Query End:129 Subject Strand: POSITIVE Subject Start: 51 Subject End: 124 .......................................................atgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... .......................................................atgaggaaagtccatgctagcactggctgtgatgccagtagtgtttgtgctaggcgaaaaaataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-20 bit: 101.0 Len: 127 Query Start:156 Query End:282 Subject Strand: POSITIVE Subject Start: 155 Subject End: 281 ...........................................................................................................................................................ggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatgg........................................................................................................................................................................................................... ...........................................................................................................................................................ggctaagtctttggatatgcctgaatagctttgaaagtgccacagtgacgtagtttctagggaaatctagaaagtggaacgcggtaaacccctcaagctagcaacccaaactttggtaggggcatgg........................................................................................................................................................................................................... HSP 3 e-value: 2.0E-11 bit: 71.9 Len: 36 Query Start:376 Query End:411 Subject Strand: POSITIVE Subject Start: 361 Subject End: 396 .......................................................................................................................................................................................................................................................................................................................................................................................ttctggaacaaaacatggcttatagaaaattgcata.......................................................................... .......................................................................................................................................................................................................................................................................................................................................................................................ttctggaacaaaacatggcttatagaaaattgcata.......................................................................... Subject: NC_004368_gbs0291_gbs0292|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[313590,314058]|469 HSP 1 e-value: 1.0E-24 bit: 115.0 Len: 74 Query Start:56 Query End:129 Subject Strand: POSITIVE Subject Start: 51 Subject End: 124 .......................................................atgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... .......................................................atgaggaaagtccatgctagcactggctgtgatgccagtagtgtttgtgctaggcgaaaaaataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-20 bit: 101.0 Len: 127 Query Start:156 Query End:282 Subject Strand: POSITIVE Subject Start: 155 Subject End: 281 ...........................................................................................................................................................ggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatgg........................................................................................................................................................................................................... ...........................................................................................................................................................ggctaagtctttggatatgcctgaatagctttgaaagtgccacagtgacgtagtttctagggaaatctagaaagtggaacgcggtaaacccctcaagctagcaacccaaactttggtaggggcatgg........................................................................................................................................................................................................... HSP 3 e-value: 2.0E-11 bit: 71.9 Len: 36 Query Start:376 Query End:411 Subject Strand: POSITIVE Subject Start: 361 Subject End: 396 .......................................................................................................................................................................................................................................................................................................................................................................................ttctggaacaaaacatggcttatagaaaattgcata.......................................................................... .......................................................................................................................................................................................................................................................................................................................................................................................ttctggaacaaaacatggcttatagaaaattgcata.......................................................................... Subject: NC_004116_SAG0302_SAG0303|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[311650,312118]|469 HSP 1 e-value: 1.0E-24 bit: 115.0 Len: 74 Query Start:56 Query End:129 Subject Strand: POSITIVE Subject Start: 51 Subject End: 124 .......................................................atgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... .......................................................atgaggaaagtccatgctagcactggctgtgatgccagtagtgtttgtgctaggcgaaaaaataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-20 bit: 101.0 Len: 127 Query Start:156 Query End:282 Subject Strand: POSITIVE Subject Start: 155 Subject End: 281 ...........................................................................................................................................................ggctaagtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatgg........................................................................................................................................................................................................... ...........................................................................................................................................................ggctaagtctttggatatgcctgaatagctttgaaagtgccacagtgacgtagtttctagggaaatctagaaagtggaacgcggtaaacccctcaagctagcaacccaaactttggtaggggcatgg........................................................................................................................................................................................................... HSP 3 e-value: 2.0E-11 bit: 71.9 Len: 36 Query Start:376 Query End:411 Subject Strand: POSITIVE Subject Start: 361 Subject End: 396 .......................................................................................................................................................................................................................................................................................................................................................................................ttctggaacaaaacatggcttatagaaaattgcata.......................................................................... .......................................................................................................................................................................................................................................................................................................................................................................................ttctggaacaaaacatggcttatagaaaattgcata.......................................................................... Subject: NC_008024_MGAS10750_Spy1460_MGAS10750_Spy1461|Methyltransferase:Cel l division initiation protein DivIVA|NEGATIVE:NEGATIVE|[1397176,1397619]|444 HSP 1 e-value: 2.0E-23 bit: 111.0 Len: 96 Query Start:187 Query End:282 Subject Strand: NEGATIVE Subject Start: 171 Subject End: 266 ..........................................................................................................................................................................................tgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatgg........................................................................................................................................................................................................... ..........................................................................................................................................................................................tgaaagtgccacagtgacgtagtttttatggaaacgtaaaaaatggaacgcggtaaacccctcaagctagcaacccaaactttggtaggggcatgg........................................................................................................................................................................................................... HSP 2 e-value: 4.0E-22 bit: 107.0 Len: 74 Query Start:56 Query End:129 Subject Strand: NEGATIVE Subject Start: 326 Subject End: 399 .......................................................atgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... .......................................................atgaggaaagtccatgctagcactggctgtgatgccagtagtgattgtgctaggcgaacacataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 8.0E-14 bit: 79.8 Len: 75 Query Start:337 Query End:411 Subject Strand: NEGATIVE Subject Start: 54 Subject End: 129 ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcaaaggaagtggt-cctagtcacttctggaacaaaacatggcttatagaaaattgcata.......................................................................... ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcgaaggaaataattcctagttatttccggaacaaaacatggcttatagaaaattgcata.......................................................................... Subject: NC_008023_MGAS2096_Spy1373_MGAS2096_Spy1374|Methyltransferase:Cell division initiation protein DivIVA|NEGATIVE:NEGATIVE|[1338520,1338963]|444 HSP 1 e-value: 2.0E-23 bit: 111.0 Len: 96 Query Start:187 Query End:282 Subject Strand: NEGATIVE Subject Start: 171 Subject End: 266 ..........................................................................................................................................................................................tgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatgg........................................................................................................................................................................................................... ..........................................................................................................................................................................................tgaaagtgccacagtgacgtagtttttatggaaacgtaaaaaatggaacgcggtaaacccctcaagctagcaacccaaactttggtaggggcatgg........................................................................................................................................................................................................... HSP 2 e-value: 4.0E-22 bit: 107.0 Len: 74 Query Start:56 Query End:129 Subject Strand: NEGATIVE Subject Start: 326 Subject End: 399 .......................................................atgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... .......................................................atgaggaaagtccatgctagcactggctgtgatgccagtagtgattgtgctaggcgaacacataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 8.0E-14 bit: 79.8 Len: 75 Query Start:337 Query End:411 Subject Strand: NEGATIVE Subject Start: 54 Subject End: 129 ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcaaaggaagtggt-cctagtcacttctggaacaaaacatggcttatagaaaattgcata.......................................................................... ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcgaaggaaataattcctagttatttccggaacaaaacatggcttatagaaaattgcata.......................................................................... Subject: NC_008022_MGAS10270_Spy1468_MGAS10270_Spy1469|Methyltransferase:Cel l division initiation protein DivIVA|NEGATIVE:NEGATIVE|[1413978,1414421]|444 HSP 1 e-value: 2.0E-23 bit: 111.0 Len: 96 Query Start:187 Query End:282 Subject Strand: NEGATIVE Subject Start: 171 Subject End: 266 ..........................................................................................................................................................................................tgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatgg........................................................................................................................................................................................................... ..........................................................................................................................................................................................tgaaagtgccacagtgacgtagtttttatggaaacgtaaaaaatggaacgcggtaaacccctcaagctagcaacccaaactttggtaggggcatgg........................................................................................................................................................................................................... HSP 2 e-value: 4.0E-22 bit: 107.0 Len: 74 Query Start:56 Query End:129 Subject Strand: NEGATIVE Subject Start: 326 Subject End: 399 .......................................................atgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... .......................................................atgaggaaagtccatgctagcactggctgtgatgccagtagtgattgtgctaggcgaacacataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 8.0E-14 bit: 79.8 Len: 75 Query Start:337 Query End:411 Subject Strand: NEGATIVE Subject Start: 54 Subject End: 129 ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcaaaggaagtggt-cctagtcacttctggaacaaaacatggcttatagaaaattgcata.......................................................................... ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcgaaggaaataattcctagttatttccggaacaaaacatggcttatagaaaattgcata.......................................................................... Subject: NC_008021_MGAS9429_Spy1347_MGAS9429_Spy1348|methyltransferase:cell division initiation protein|NEGATIVE:NEGATIVE|[1314678,1315121]|444 HSP 1 e-value: 2.0E-23 bit: 111.0 Len: 96 Query Start:187 Query End:282 Subject Strand: NEGATIVE Subject Start: 171 Subject End: 266 ..........................................................................................................................................................................................tgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatgg........................................................................................................................................................................................................... ..........................................................................................................................................................................................tgaaagtgccacagtgacgtagtttttatggaaacgtaaaaaatggaacgcggtaaacccctcaagctagcaacccaaactttggtaggggcatgg........................................................................................................................................................................................................... HSP 2 e-value: 4.0E-22 bit: 107.0 Len: 74 Query Start:56 Query End:129 Subject Strand: NEGATIVE Subject Start: 326 Subject End: 399 .......................................................atgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... .......................................................atgaggaaagtccatgctagcactggctgtgatgccagtagtgattgtgctaggcgaacacataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 8.0E-14 bit: 79.8 Len: 75 Query Start:337 Query End:411 Subject Strand: NEGATIVE Subject Start: 54 Subject End: 129 ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcaaaggaagtggt-cctagtcacttctggaacaaaacatggcttatagaaaattgcata.......................................................................... ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcgaaggaaataattcctagttatttccggaacaaaacatggcttatagaaaattgcata.......................................................................... Subject: NC_007297_M5005_Spy_1351_M5005_Spy_1352|methyltransferase:cell division initiation protein|NEGATIVE:NEGATIVE|[1320078,1320521]|444 HSP 1 e-value: 2.0E-23 bit: 111.0 Len: 96 Query Start:187 Query End:282 Subject Strand: NEGATIVE Subject Start: 171 Subject End: 266 ..........................................................................................................................................................................................tgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatgg........................................................................................................................................................................................................... ..........................................................................................................................................................................................tgaaagtgccacagtgacgtagtttttatggaaacgtaaaaaatggaacgcggtaaacccctcaagctagcaacccaaactttggtaggggcatgg........................................................................................................................................................................................................... HSP 2 e-value: 4.0E-22 bit: 107.0 Len: 74 Query Start:56 Query End:129 Subject Strand: NEGATIVE Subject Start: 326 Subject End: 399 .......................................................atgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... .......................................................atgaggaaagtccatgctagcactggctgtgatgccagtagtgattgtgctaggcgaacacataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 8.0E-14 bit: 79.8 Len: 75 Query Start:337 Query End:411 Subject Strand: NEGATIVE Subject Start: 54 Subject End: 129 ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcaaaggaagtggt-cctagtcacttctggaacaaaacatggcttatagaaaattgcata.......................................................................... ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcgaaggaaataattcctagttatttccggaacaaaacatggcttatagaaaattgcata.......................................................................... Subject: NC_007296_M28_Spy1392_M28_Spy1393|methyltransferase:cell division initiation protein DivIVA|NEGATIVE:NEGATIVE|[1389732,1390175]|444 HSP 1 e-value: 2.0E-23 bit: 111.0 Len: 96 Query Start:187 Query End:282 Subject Strand: NEGATIVE Subject Start: 171 Subject End: 266 ..........................................................................................................................................................................................tgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatgg........................................................................................................................................................................................................... ..........................................................................................................................................................................................tgaaagtgccacagtgacgtagtttttatggaaacgtaaaaaatggaacgcggtaaacccctcaagctagcaacccaaactttggtaggggcatgg........................................................................................................................................................................................................... HSP 2 e-value: 4.0E-22 bit: 107.0 Len: 74 Query Start:56 Query End:129 Subject Strand: NEGATIVE Subject Start: 326 Subject End: 399 .......................................................atgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... .......................................................atgaggaaagtccatgctagcactggctgtgatgccagtagtgattgtgctaggcgaacacataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 8.0E-14 bit: 79.8 Len: 75 Query Start:337 Query End:411 Subject Strand: NEGATIVE Subject Start: 54 Subject End: 129 ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcaaaggaagtggt-cctagtcacttctggaacaaaacatggcttatagaaaattgcata.......................................................................... ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcgaaggaaataattcctagttatttccggaacaaaacatggcttatagaaaattgcata.......................................................................... Subject: NC_006086_M6_Spy1397_M6_Spy1398|Methyltransferase:Cell division initiation protein|NEGATIVE:NEGATIVE|[1392201,1392644]|444 HSP 1 e-value: 2.0E-23 bit: 111.0 Len: 96 Query Start:187 Query End:282 Subject Strand: NEGATIVE Subject Start: 171 Subject End: 266 ..........................................................................................................................................................................................tgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatgg........................................................................................................................................................................................................... ..........................................................................................................................................................................................tgaaagtgccacagtgacgtagtttttatggaaacgtaaaaaatggaacgcggtaaacccctcaagctagcaacccaaactttggtaggggcatgg........................................................................................................................................................................................................... HSP 2 e-value: 4.0E-22 bit: 107.0 Len: 74 Query Start:56 Query End:129 Subject Strand: NEGATIVE Subject Start: 326 Subject End: 399 .......................................................atgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... .......................................................atgaggaaagtccatgctagcactggctgtgatgccagtagtgattgtgctaggcgaacacataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 8.0E-14 bit: 79.8 Len: 75 Query Start:337 Query End:411 Subject Strand: NEGATIVE Subject Start: 54 Subject End: 129 ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcaaaggaagtggt-cctagtcacttctggaacaaaacatggcttatagaaaattgcata.......................................................................... ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcgaaggaaataattcctagttatttccggaacaaaacatggcttatagaaaattgcata.......................................................................... Subject: NC_004606_SPs0475_SPs0476|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[507839,508282]|444 HSP 1 e-value: 2.0E-23 bit: 111.0 Len: 96 Query Start:187 Query End:282 Subject Strand: POSITIVE Subject Start: 179 Subject End: 274 ..........................................................................................................................................................................................tgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatgg........................................................................................................................................................................................................... ..........................................................................................................................................................................................tgaaagtgccacagtgacgtagtttttatggaaacgtaaaaaatggaacgcggtaaacccctcaagctagcaacccaaactttggtaggggcatgg........................................................................................................................................................................................................... HSP 2 e-value: 4.0E-22 bit: 107.0 Len: 74 Query Start:56 Query End:129 Subject Strand: POSITIVE Subject Start: 46 Subject End: 119 .......................................................atgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... .......................................................atgaggaaagtccatgctagcactggctgtgatgccagtagtgattgtgctaggcgaacacataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 8.0E-14 bit: 79.8 Len: 75 Query Start:337 Query End:411 Subject Strand: POSITIVE Subject Start: 316 Subject End: 391 ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcaaaggaagtggt-cctagtcacttctggaacaaaacatggcttatagaaaattgcata.......................................................................... ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcgaaggaaataattcctagttatttccggaacaaaacatggcttatagaaaattgcata.......................................................................... Subject: NC_004070_SpyM3_1386_SpyM3_1387|putative N6-adenine-specific DNA methylase:hypothetical protein|NEGATIVE:NEGATIVE|[1388604,1389047]|444 HSP 1 e-value: 2.0E-23 bit: 111.0 Len: 96 Query Start:187 Query End:282 Subject Strand: NEGATIVE Subject Start: 171 Subject End: 266 ..........................................................................................................................................................................................tgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatgg........................................................................................................................................................................................................... ..........................................................................................................................................................................................tgaaagtgccacagtgacgtagtttttatggaaacgtaaaaaatggaacgcggtaaacccctcaagctagcaacccaaactttggtaggggcatgg........................................................................................................................................................................................................... HSP 2 e-value: 4.0E-22 bit: 107.0 Len: 74 Query Start:56 Query End:129 Subject Strand: NEGATIVE Subject Start: 326 Subject End: 399 .......................................................atgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... .......................................................atgaggaaagtccatgctagcactggctgtgatgccagtagtgattgtgctaggcgaacacataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 8.0E-14 bit: 79.8 Len: 75 Query Start:337 Query End:411 Subject Strand: NEGATIVE Subject Start: 54 Subject End: 129 ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcaaaggaagtggt-cctagtcacttctggaacaaaacatggcttatagaaaattgcata.......................................................................... ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcgaaggaaataattcctagttatttccggaacaaaacatggcttatagaaaattgcata.......................................................................... Subject: NC_003485_spyM18_1656_spyM18_1658|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1378153,1378596]|444 HSP 1 e-value: 2.0E-23 bit: 111.0 Len: 96 Query Start:187 Query End:282 Subject Strand: NEGATIVE Subject Start: 171 Subject End: 266 ..........................................................................................................................................................................................tgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatgg........................................................................................................................................................................................................... ..........................................................................................................................................................................................tgaaagtgccacagtgacgtagtttttatggaaacgtaaaaaatggaacgcggtaaacccctcaagctagcaacccaaactttggtaggggcatgg........................................................................................................................................................................................................... HSP 2 e-value: 4.0E-22 bit: 107.0 Len: 74 Query Start:56 Query End:129 Subject Strand: NEGATIVE Subject Start: 326 Subject End: 399 .......................................................atgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... .......................................................atgaggaaagtccatgctagcactggctgtgatgccagtagtgattgtgctaggcgaacacataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 8.0E-14 bit: 79.8 Len: 75 Query Start:337 Query End:411 Subject Strand: NEGATIVE Subject Start: 54 Subject End: 129 ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcaaaggaagtggt-cctagtcacttctggaacaaaacatggcttatagaaaattgcata.......................................................................... ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcgaaggaaataattcctagttatttccggaacaaaacatggcttatagaaaattgcata.......................................................................... Subject: NC_002737_SPy1644_SPy1646|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1365118,1365561]|444 HSP 1 e-value: 2.0E-23 bit: 111.0 Len: 96 Query Start:187 Query End:282 Subject Strand: NEGATIVE Subject Start: 171 Subject End: 266 ..........................................................................................................................................................................................tgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatgg........................................................................................................................................................................................................... ..........................................................................................................................................................................................tgaaagtgccacagtgacgtagtttttatggaaacgtaaaaaatggaacgcggtaaacccctcaagctagcaacccaaactttggtaggggcatgg........................................................................................................................................................................................................... HSP 2 e-value: 4.0E-22 bit: 107.0 Len: 74 Query Start:56 Query End:129 Subject Strand: NEGATIVE Subject Start: 326 Subject End: 399 .......................................................atgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... .......................................................atgaggaaagtccatgctagcactggctgtgatgccagtagtgattgtgctaggcgaacacataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 8.0E-14 bit: 79.8 Len: 75 Query Start:337 Query End:411 Subject Strand: NEGATIVE Subject Start: 54 Subject End: 129 ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcaaaggaagtggt-cctagtcacttctggaacaaaacatggcttatagaaaattgcata.......................................................................... ................................................................................................................................................................................................................................................................................................................................................ggtagacagatgattatcgaaggaaataattcctagttatttccggaacaaaacatggcttatagaaaattgcata.......................................................................... Subject: NC_000964_BSU22170_BSU22180|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[2330430,2330975]|546 HSP 1 e-value: 6.0E-21 bit: 103.0 Len: 116 Query Start:162 Query End:277 Subject Strand: NEGATIVE Subject Start: 224 Subject End: 339 .................................................................................................................................................................gtcttcggataggtcagagtaggcttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcgggg................................................................................................................................................................................................................ .................................................................................................................................................................gtcttcggatatggctgagtatccttgaaagtgccacagtgacgaagtctcactagaaatggtgagagtggaacgcggtaaacccctcgagcgagaaacccaaattttggtagggg................................................................................................................................................................................................................ Subject: NC_004350_SMU.471_SMU.472|hypothetical protein:conserved hypothetical protein; possible N6-adenine-specific DNA methylase|POSITIVE:POSITIVE|[439785,440233]|449 HSP 1 e-value: 3.0E-19 bit: 97.6 Len: 97 Query Start:187 Query End:283 Subject Strand: POSITIVE Subject Start: 190 Subject End: 286 ..........................................................................................................................................................................................tgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaatttcggtcggggcatgga.......................................................................................................................................................................................................... ..........................................................................................................................................................................................tgaaagtgccacagtgacgtagcttttatggaaacataaaaggtggaacgcggtaaacccctcaagctagcaacccaaactttggtaggggcatgga.......................................................................................................................................................................................................... HSP 2 e-value: 1.0E-18 bit: 95.6 Len: 76 Query Start:54 Query End:129 Subject Strand: POSITIVE Subject Start: 55 Subject End: 130 .....................................................tcatgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagcctaggga.................................................................................................................................................................................................................................................................................................................................................................... .....................................................tcatgaggaaagtccatgctagcactggctgtgatgccagtagtgtttgtgctagacaaaaaaataagtctaggga.................................................................................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 1.0E-15 bit: 85.7 Len: 74 Query Start:338 Query End:411 Subject Strand: POSITIVE Subject Start: 327 Subject End: 401 .................................................................................................................................................................................................................................................................................................................................................gtagacagatgattatcaaaggaagtggt-cctagtcacttctggaacaaaacatggcttatagaaaattgcata.......................................................................... .................................................................................................................................................................................................................................................................................................................................................gtagacagatgattatcaaaaaaggtggtacctagtcgcctttggaacaaaacatggcttatagaaaattgcata.......................................................................... Subject: NC_002570_BH1770_BH1771|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1843260,1843947]|688 HSP 1 e-value: 2.0E-11 bit: 71.9 Len: 80 Query Start:186 Query End:265 Subject Strand: POSITIVE Subject Start: 234 Subject End: 313 .........................................................................................................................................................................................ttgaaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaa............................................................................................................................................................................................................................ .........................................................................................................................................................................................ttgaaagtgccacagtgacgtagtccgtttggaaacagacggagtggaacgaggtaaacccctcgagcgagaaacccaaa............................................................................................................................................................................................................................ HSP 2 e-value: 5.0E-9 bit: 63.9 Len: 48 Query Start:58 Query End:105 Subject Strand: POSITIVE Subject Start: 92 Subject End: 139 .........................................................gaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgc............................................................................................................................................................................................................................................................................................................................................................................................ .........................................................gaggaaagtccatgctcgcacaggctgagatgcttgtagtgttcgtgc............................................................................................................................................................................................................................................................................................................................................................................................ Subject: NC_004668_EF1151_EF1152|cell division protein DivIVA, putative:hypothetical protein|POSITIVE:POSITIVE|[1121852,1122564]|713 HSP 1 e-value: 3.0E-10 bit: 67.9 Len: 66 Query Start:57 Query End:122 Subject Strand: POSITIVE Subject Start: 141 Subject End: 206 ........................................................tgaggaaagtccatgctagcacaggctgtgatgcctgtagtgtttgtgctaggcgaaaccataagc........................................................................................................................................................................................................................................................................................................................................................................... ........................................................tgaggaaagtccatgctcgcacaagctgagatgcttgtagtgttcgtgcttagcgaaatcataagc........................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 3.0E-7 bit: 58.0 Len: 37 Query Start:229 Query End:265 Subject Strand: POSITIVE Subject Start: 314 Subject End: 350 ....................................................................................................................................................................................................................................gtggaacgcggtaaacccctcaagctagcaacccaaa............................................................................................................................................................................................................................ ....................................................................................................................................................................................................................................gtggaacgcggtaaacccctcgagcgagcaacccaaa............................................................................................................................................................................................................................ Subject: NC_009004_llmg_0769_llmg_0771|Putative DivIVA cell division initiation protein:Putative N6-adenine-specific DNA methylase|POSITIVE:POSITIVE|[758918,759552]|635 HSP 1 e-value: 3.0E-7 bit: 58.0 Len: 77 Query Start:189 Query End:265 Subject Strand: POSITIVE Subject Start: 305 Subject End: 380 ............................................................................................................................................................................................aaagtgccacagtgacggagtctttctggaaacggagagagtggaacgcggtaaacccctcaagctagcaacccaaa............................................................................................................................................................................................................................ ............................................................................................................................................................................................aaagtgccatagagacg-agtcttagtggaaacgctaagaatggaacgtggtaaacccctcaagcgagcaacccaaa............................................................................................................................................................................................................................ HSP 2 e-value: 5.0E-6 bit: 54.0 Len: 27 Query Start:378 Query End:404 Subject Strand: POSITIVE Subject Start: 492 Subject End: 518 .........................................................................................................................................................................................................................................................................................................................................................................................ctggaacaaaacatggcttatagaaaa................................................................................. .........................................................................................................................................................................................................................................................................................................................................................................................ctggaacaaaacatggcttatagaaaa................................................................................. Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAUUUGAAAAGUGCAAUUUUUGGAUAAUCGCGUGAGGAGAAUUUCUUUUCAUGAGGAAAGUCCAUGCUAGCACAGGCUGUGAUGCCUGUAGUGUUUGUGCUAGGCGAAACCAUAAGCCUAGGGACGAGAGAUCGUUACGGCAGUUGAAAUGGCUAAGUCUUCGGAUAGGUCAGAGUAGGCUUGAAAGUGCCACAGUGACGGAGUCUUUCUGGAAACGGAGAGAGUGGAACGCGGUAAACCCCUCAAGCUAGCAACCCAAAUUUCGGUCGGGGCAUGGAGUGCACGGAAACGAACGUAGUACUCUGACUGCUAUCAGCUAUAGGCUGUUAGUGGUAGACAGAUGAUUAUCAAAGGAAGUGGUCCUAGUCACUUCUGGAACAAAACAUGGCUUAUAGAAAAUUGCAUAUAGGUUGGGGCUGAGAAAUUUUCUCAACCUCAUUUUUUAAAGUGGACAUAUAGAAAGGUCUUAUAAGACUG ...(((((((.(((((((((((.(((((((.(((((((((.....)))))))))......(((..(((((((((((((...............)))))))))).)))..(((((.((((((..(((((....))))).....((((((..((((...(((....))).(((((((((((((((((...((((.(.((..((...((((((((....)))))))).))..))).))))......))))))).(((..(((..(..(....)..)..)))..))).((....)).......)))))))))))))))))))).))))))....))))).)))....)))))))...((((((((......))))))))....................)))))))))))......(((((.(((((.....))))).)))))..)))))))...............(((((....))))). (-158.16) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAGUCUUAUAAGACCUUUCUAUAUGUCCACUUUAAAAAAUGAGGUUGAGAAAAUUUCUCAGCCCCAACCUAUAUGCAAUUUUCUAUAAGCCAUGUUUUGUUCCAGAAGUGACUAGGACCACUUCCUUUGAUAAUCAUCUGUCUACCACUAACAGCCUAUAGCUGAUAGCAGUCAGAGUACUACGUUCGUUUCCGUGCACUCCAUGCCCCGACCGAAAUUUGGGUUGCUAGCUUGAGGGGUUUACCGCGUUCCACUCUCUCCGUUUCCAGAAAGACUCCGUCACUGUGGCACUUUCAAGCCUACUCUGACCUAUCCGAAGACUUAGCCAUUUCAACUGCCGUAACGAUCUCUCGUCCCUAGGCUUAUGGUUUCGCCUAGCACAAACACUACAGGCAUCACAGCCUGUGCUAGCAUGGACUUUCCUCAUGAAAAGAAAUUCUCCUCACGCGAUUAUCCAAAAAUUGCACUUUUCAAAUAA ..((((....))))..........(((((..........((.((((((((.....)))))))).))................(((((((((.............((((((.(((((....((((....((((...............(((.((((.....)))).)))..(((((((((....((.((....)).)).......(((((((.......)))))..))..(((((((((.....((((.........(((........)))..(((...)))...))))..))))))))).))))))))).)))).)))).))))).)))))).......(..((((....))))..)..)))))))))....((.(((((((.........(((......)))))))))))).)))))........(((((((.............((((((.......)))))).)))))))..... (-115.14) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 376 219 171 114 72 smit:SMT_1922|5end_exonuclease_RexA_1865998..1866228_NEGATIVE 353 276 239 45 11 smit:SMT_2050|5end_K03712_MarR_family_transcriptional_regulator_1959973..1960203_NEGATIVE 348 279 231 117 52 smit:SMT_1793|5end_inosine-5'-monophosphate_dehydrogenase_1767985..1768215_POSITIVE 346 246 204 152 108 smit:SMT_235|5end_hypothetical_protein_174081..174311_POSITIVE 338 99 52 99 45 smit:SMT_450|5end_hydroxyethylthiazole_kinase_388139..388369_POSITIVE 335 287 243 211 172 smit:SMT_499|5end_hypothetical_protein_432572..432802_POSITIVE 333 142 101 205 170 smit:SMT_1668|5end_K+_transport_system,_NAD-binding_component_1658288..1658518_NEGATIVE 333 110 63 97 40 smit:SMT_1221|5end_choline_binding_protein_A_1185539..1185769_NEGATIVE 333 198 152 201 146 smit:SMT_849|5end_hypothetical_protein_796127..796357_NEGATIVE 328 150 103 95 44 smit:SMT_1146|5end_maltose_operon_transcriptional_repressor_1090136..1090366_POSITIVE 326 250 207 104 57 smit:SMT_1835|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1803894..1804124_POSITIVE 326 286 242 169 134 smit:SMT_1245|5end_SOS_responce_UmuC_protein_1205120..1205350_POSITIVE 325 219 171 114 72 smit:SMT_1484|5end_exonuclease_RexA_1464936..1465166_NEGATIVE 325 80 31 193 147 smit:SMT_466|5end_UDP-N-acetylmuramoylalanine--D-glutamate_ligase_403645..403875_POSITIVE 323 120 71 71 24 smit:SMT_412|5end_hypothetical_protein_predicted_by_Glimmer/Critica_356606..356836_NEGATIVE 321 128 83 152 104 smit:SMT_1537|5end_hypothetical_protein_1522668..1522898_NEGATIVE 320 310 265 164 107 smit:SMT_2052|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1961591..1961821_NEGATIVE 317 106 63 219 178 smit:SMT_2016|5end_tyrosine_recombinase_1933425..1933655_NEGATIVE 317 106 63 192 151 smit:SMT_2008|5end_K07485_transposase_1928662..1928892_NEGATIVE 317 106 63 61 20 smit:SMT_2005|5end_pullulanase,_type_I_1926289..1926519_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 356 244 202 82 34 smit:SMT_82|3end_transcriptional_regulator,_TetR_family_14330..14480_POSITIVE 351 210 163 56 8 smit:SMT_1920|3end_ABC_transporter,_ATP-binding_protein_1862736..1862886_NEGATIVE 346 246 204 142 98 smit:SMT_234|3end_aminopeptidase_PepS_174151..174301_POSITIVE 338 99 52 99 45 smit:SMT_449|3end_hypothetical_protein_388219..388369_POSITIVE 330 109 67 56 20 smit:SMT_2109|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1999061..1999211_NEGATIVE 330 109 67 56 20 smit:SMT_1180|3end_putative_tagatose-6-phosphate_aldose/ketose_isomerase_1138036..1138186_NEGATIVE 328 150 103 85 34 smit:SMT_1145|3end_maltodextrose_utilization_protein_MalA_1090206..1090356_POSITIVE 326 286 242 118 83 smit:SMT_1244|3end_putative_repressor_protein_1205149..1205299_POSITIVE 321 128 83 151 103 smit:SMT_1538|3end_two-component_response_regulator_YvqC_1522669..1522819_NEGATIVE 317 106 63 84 39 smit:SMT_1941|3end_hypothetical_protein_1877663..1877813_POSITIVE 312 199 151 61 2 smit:SMT_413|3end_tRNA_delta(2)-isopentenylpyrophosphate_transferase_357641..357791_POSITIVE 311 287 242 56 10 smit:SMT_312|3end_acetyl-CoA_carboxylase,_biotin_carboxyl_carrier_protein_253926..254076_POSITIVE 309 200 152 51 5 smit:SMT_198|3end_50S_ribosomal_protein_L17_129281..129431_POSITIVE 308 258 222 74 36 smit:SMT_895|3end_cysteinyl-tRNA_synthetase_841694..841844_NEGATIVE 307 109 67 56 20 smit:SMT_1809|3end_MutT/NUDIX_family_protein_1785134..1785284_POSITIVE 303 199 157 145 93 smit:SMT_1426|3end_K03095_SprT-like_protein_1410864..1411014_NEGATIVE 303 120 75 81 29 smit:SMT_750|3end_3-phosphoshikimate_1-carboxyvinyltransferase_653964..654114_NEGATIVE 301 116 76 112 65 smit:SMT_914|3end_sucrose-6-phosphate_hydrolase_867673..867823_POSITIVE 301 109 71 58 14 smit:SMT_255|3end_K02026_multiple_sugar_transport_system_permease_protein_192707..192857_POSITIVE 300 250 202 82 31 smit:SMT_1124|3end_pyrrolidone-carboxylate_peptidase_1060225..1060375_NEGATIVE