Origin IGS: gggtgatttctcctaaatgaacgatgctattttgtttcttttacgaattgatagagataataaagataagtaatagaagaaaggatagcgatagtgagcagtaaataataattccaattgcctgagacgagcatcagttgtggaaaagatagaattataaagtatagtgctaagttgagtatgcgtgtttttggggagttaatctttagataagttagtcctttattaagtcctggaaaaaagactagcacgaggaatatctctctgattggcaagttccctgaaacgatgatgaagatgagcagagaactgaacaaaagatgataggtaagtaaagttactagtgatttcatagggcacctc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gaggtgccctatgaaatcactagtaactttacttacctatcatcttttgttcagttctctgctcatcttcatcatcgtttcagggaacttgccaatcagagagatattcctcgtgctagtcttttttccaggacttaataaaggactaacttatctaaagattaactccccaaaaacacgcatactcaacttagcactatactttataattctatcttttccacaactgatgctcgtctcaggcaattggaattattatttactgctcactatcgctatcctttcttctattacttatctttattatctctatcaattcgtaaaagaaacaaaatagcatcgttcatttaggagaaatcaccc Mask Tandem Repeat Region ================================================ gggtgatttctcctaaatgaacgatgctattttgtttcttttacgaattgatagagataataaagataagtaatagaagaaaggatagcgatagtgagcagtaaataataattccaattgcctgagacgagcatcagttgtggaaaagatagaattataaagtatagtgctaagttgagtatgcgtgtttttggggagttaatctttagataagttagtcctttattaagtcctggaaaaaagactagcacgaggaatatctctctgattggcaagttccctgaaacgatgatgaagatgagcagagaactgaacaaaagatgataggtaagtaaagttactagtgatttcatagggcacctc Find is-nt database================================================ Query_seq: SMT_263:SMT_264|SMT_263:SMT_264:hypothetical protein predicted by Glimmer/Critica:hypothetical protein:<-<-:203048..203410 363 gggtgatttctcctaaatgaacgatgctattttgtttcttttacgaattgatagagataataaagataagtaatagaagaaaggatagcgatagtgagcagtaaataataattccaattgcctgagacgagcatcagttgtggaaaagatagaattataaagtatagtgctaagttgagtatgcgtgtttttggggagttaatctttagataagttagtcctttattaagtcctggaaaaaagactagcacgaggaatatctctctgattggcaagttccctgaaacgatgatgaagatgagcagagaactgaacaaaagatgataggtaagtaaagttactagtgatttcatagggcacctc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_263:SMT_264|SMT_263:SMT_264:hypothetical protein predicted by Glimmer/Critica:hypothetical protein:<-<-:203048..203410 363 gggtgatttctcctaaatgaacgatgctattttgtttcttttacgaattgatagagataataaagataagtaatagaagaaaggatagcgatagtgagcagtaaataataattccaattgcctgagacgagcatcagttgtggaaaagatagaattataaagtatagtgctaagttgagtatgcgtgtttttggggagttaatctttagataagttagtcctttattaagtcctggaaaaaagactagcacgaggaatatctctctgattggcaagttccctgaaacgatgatgaagatgagcagagaactgaacaaaagatgataggtaagtaaagttactagtgatttcatagggcacctc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_263:SMT_264|SMT_263:SMT_264:hypothetical protein predicted by Glimmer/Critica:hypothetical protein:<-<-:203048..203410 363 gggtgatttctcctaaatgaacgatgctattttgtttcttttacgaattgatagagataataaagataagtaatagaagaaaggatagcgatagtgagcagtaaataataattccaattgcctgagacgagcatcagttgtggaaaagatagaattataaagtatagtgctaagttgagtatgcgtgtttttggggagttaatctttagataagttagtcctttattaagtcctggaaaaaagactagcacgaggaatatctctctgattggcaagttccctgaaacgatgatgaagatgagcagagaactgaacaaaagatgataggtaagtaaagttactagtgatttcatagggcacctc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 114 Max: 362 Len: 249 Subject: UniRef90_Q2MGH3 Cluster: Hypothetical protein; n=1; Streptococcus pneumoniae|Rep: Hypothetical protein - Streptococcus pneumoniae HSP 1 e-value: 2.0E-6 bit: 53.9 Len: 249 Query Start:114 Query End:362 Subject Strand: null Subject Start: 17 Subject End: 97 ................................................................................................................. R C P M K S L V X X X X X X X X X X X X X X X X I V S G N L P I R E I F L V L V F F P G L N K G L T Y L K I N S P K T R I L N L A L Y F I I L S F P Q L M L V S G N W . ................................................................................................................. R C L M K S L A R L L I I H V F I S I F L F F A L T S G A I S H T V L L L L L L F L P A L N K G L E - - K I Q S K R I P V L N A A L F F L L I S F P Q L L T N P V Q W . gggtgatttctcctaaatgaacgatgctattttgtttcttttacgaattgatagagataataaagataagtaatagaagaaaggatagcgatagtgagcagtaaataataattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnc Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 118 End: 210 ..................................................................................................................... M P E T S I S C G K D R I I K Y S A K L S M R V F G E L I F R ......................................................................................................................................................... Protein_Len: 44 Strand: - Start: 229 End: 360 .................................................................................................................................................................................................................................... T R S F L S A R P I D R Q N A L E R F R H H L H A S F Q V F S S L Y T F N S T I E Y P M ... Protein_Len: 60 Strand: - Start: 30 End: 353 ............................. N L I R T K P S N I K L Y T L G K N L G P F F V L V L F I E R I P L N G S V I I F I L L S S F L L H Y T L L T V L S K M .......... Protein_Len: 50 Strand: - Start: 16 End: 165 ............... I F S A I K N R K R I S L S L L S L Y Y F F P Y R Y H A T F L L E L Q R L R A D T T S F I S N Y L M ...................................................................................................................................................................................................... gggtgatttctcctaaatgaacgatgctattttgtttcttttacgaattgatagagataataaagataagtaatagaagaaaggatagcgatagtgagcagtaaataataattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gggtgatttctcctaaatgaacgatgctattttgtttcttttacgaattgatagagataataaagataagtaatagaagaaaggatagcgatagtgagcagtaaataataattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_263:SMT_264|SMT_263:SMT_264:hypothetical protein predicted by Glimmer/Critica:hypothetical protein:<-<-:203048..203410 363 gggtgatttctcctaaatgaacgatgctattttgtttcttttacgaattgatagagataataaagataagtaatagaagaaaggatagcgatagtgagcagtaaataataattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnc Intra-Species Hit: Count: 1 Min: 1 Max: 113 Len: 113 Subject: smit_SMT_263_SMT_264|hypothetical protein predicted by Glimmer/Critica:hypothetical protein|NEGATIVE:NEGATIVE|[203048,203410]|363 HSP 1 e-value: 2.0E-57 bit: 224.0 Len: 113 Query Start:1 Query End:113 Subject Strand: POSITIVE Subject Start: 1 Subject End: 113 gggtgatttctcctaaatgaacgatgctattttgtttcttttacgaattgatagagataataaagataagtaatagaagaaaggatagcgatagtgagcagtaaataataatt.......................................................................................................................................................................................................................................................... gggtgatttctcctaaatgaacgatgctattttgtttcttttacgaattgatagagataataaagataagtaatagaagaaaggatagcgatagtgagcagtaaataataatt.......................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGGUGAUUUCUCCUAAAUGAACGAUGCUAUUUUGUUUCUUUUACGAAUUGAUAGAGAUAAUAAAGAUAAGUAAUAGAAGAAAGGAUAGCGAUAGUGAGCAGUAAAUAAUAAUU (((.(....).)))...((.((..(((((((((.(((((.((((..(((..((.......))..)))..)))).)))))..)))))))))...))...))............. (-17.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUAUUAUUUACUGCUCACUAUCGCUAUCCUUUCUUCUAUUACUUAUCUUUAUUAUCUCUAUCAAUUCGUAAAAGAAACAAAAUAGCAUCGUUCAUUUAGGAGAAAUCACCC ........................(((((......((((.((((.........................)))).)))).....))))).............((....)).... ( -9.01) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 789 50 1 141 92 smit:SMT_263|5end_hypothetical_protein_predicted_by_Glimmer/Critica_202957..203187_NEGATIVE 246 50 1 60 12 smit:SMT_1359|5end_dihydroorotate_dehydrogenase_electron_transfer_subunit_1338003..1338233_NEGATIVE 237 41 2 55 17 smit:SMT_1122|5end_queuine_tRNA-ribosyltransferase_1059223..1059453_NEGATIVE 224 102 65 209 162 smit:SMT_1900|5end_K03710_GntR_family_transcriptional_regulator_1850208..1850438_POSITIVE 222 89 65 228 206 smit:SMT_1071|5end_hypothetical_protein_1010058..1010288_POSITIVE 214 107 67 43 1 smit:SMT_1053|5end_ABC_transporter_permease_protein_993671..993901_NEGATIVE 212 37 4 41 7 smit:SMT_1493|5end_DNA-directed_RNA_polymerase,_beta'_subunit/160_kD_subunit_1475737..1475967_POSITIVE 210 105 61 68 19 smit:SMT_755|5end_shikimate_5-dehydrogenase_659917..660147_NEGATIVE 209 43 2 106 65 smit:SMT_1517|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1498313..1498543_POSITIVE 208 100 53 214 171 smit:SMT_1477|5end_K02077_zinc/manganese_transport_system_substrate-binding_protein_1460376..1460606_NEGATIVE 208 102 64 47 6 smit:SMT_350|5end_immunity_protein_BlpL_289864..290094_POSITIVE 206 45 4 139 93 smit:SMT_1058|5end_primosomal_protein_DnaI_996486..996716_POSITIVE 206 99 53 65 13 smit:SMT_997|5end_integral_membrane_protein_938243..938473_POSITIVE 204 39 2 125 81 smit:SMT_977|5end_conserved_hypothetical_protein,_Rrf2_family_protein_925689..925919_NEGATIVE 202 89 43 61 6 smit:SMT_202|5end_K02011_iron(III)_transport_system_permease_protein_133831..134061_NEGATIVE 202 57 22 187 148 smit:SMT_102|5end_competence-specific_global_transcription_modulator_34108..34338_POSITIVE 200 45 4 152 103 smit:SMT_1322|5end_K02075_zinc/manganese_transport_system_permease_protein_1291371..1291601_POSITIVE 199 100 53 183 145 smit:SMT_1831|5end_galactose-6-phosphate_isomerase_1799912..1800142_POSITIVE 199 76 43 181 154 smit:SMT_1144|5end_K02026_multiple_sugar_transport_system_permease_protein_1088222..1088452_POSITIVE 197 99 53 231 180 smit:SMT_1909|5end_zinc_metalloprotease_ZmpB,_truncated_1856749..1856979_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 251 102 63 48 9 smit:SMT_1369|3end_alanine_dehydrogenase_1349472..1349622_POSITIVE 241 55 22 80 38 smit:SMT_2031|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1945633..1945783_NEGATIVE 210 105 61 78 29 smit:SMT_756|3end_3-dehydroquinate_dehydratase_659907..660057_NEGATIVE 206 45 4 138 92 smit:SMT_1057|3end_replicative_DNA_helicase_996565..996715_POSITIVE 204 39 2 49 5 smit:SMT_978|3end_hypothetical_protein_925765..925915_NEGATIVE 202 89 43 71 16 smit:SMT_203|3end_putative_ABC_transporter_BitD_133821..133971_NEGATIVE 202 57 22 66 27 smit:SMT_101|3end_cell_division_protein_34067..34217_POSITIVE 197 58 11 146 101 smit:SMT_826|3end_hypothetical_protein_749591..749741_NEGATIVE 194 48 7 136 84 smit:SMT_1396|3end_uracil-DNA_glycosylase_1372797..1372947_POSITIVE 192 56 11 150 98 smit:SMT_570|3end_hypothetical_protein_489179..489329_POSITIVE 192 59 27 72 31 smit:SMT_75|3end_hypothetical_protein_2754..2904_POSITIVE 187 28 1 143 109 smit:SMT_1834|3end_cell_division_protein_1802502..1802652_NEGATIVE 187 99 60 77 39 smit:SMT_1279|3end_translation_elongation_factor_Ts_1243451..1243601_POSITIVE 186 99 54 78 30 smit:SMT_1524|3end_peptidase_T_1505867..1506017_POSITIVE 182 46 6 148 93 smit:SMT_173|3end_50S_ribosomal_protein_L2_117407..117557_POSITIVE 181 43 16 32 4 smit:SMT_740|3end_50S_ribosomal_protein_L10_643715..643865_NEGATIVE 180 101 64 75 35 smit:SMT_1967|3end_dicarboxylate/amino_acid:cation_(Na+_or_H+)_symporter_(DAACS)_family_protein_1899498..1899648_NEGATIVE 180 101 64 75 35 smit:SMT_996|3end_K03309_dicarboxylate/amino_acid:cation_(Na+_or_H+)_symporter,_DAACS_family_938255..938405_POSITIVE 179 49 1 97 32 smit:SMT_1728|3end_exodeoxyribonuclease_VII_large_subunit_1714668..1714818_POSITIVE 179 63 24 130 93 smit:SMT_770|3end_K02053_spermidine/putrescine_transport_system_permease_protein_680586..680736_NEGATIVE