Origin IGS: aaatgagctttctttttttcataaatctgattttttataacttgtttggaaaataaaaaaagctarggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcrcgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tacatcgcatcctctctcttccaagtttcaaacttctattcaatatagctaaaatagggcatagctattcacagaatattataacacaaaaaatccaatcgygcttagtttttcctaaatttacttaccattttttcacattctttccytagctttttttattttccaaacaagttataaaaaatcagatttatgaaaaaaagaaagctcattt Mask Tandem Repeat Region ================================================ aaatgagctttctttttttcataaatctgattttttataacttgtttggaaaataaaaaaagctarggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcrcgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta Find is-nt database================================================ Query_seq: SMT_2162:SMT_2163|SMT_2162:SMT_2163:hypothetical protein:cell division protein FtsA:->->:2037569..2037782 214 aaatgagctttctttttttcataaatctgattttttataacttgtttggaaaataaaaaaagctarggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcrcgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_2162:SMT_2163|SMT_2162:SMT_2163:hypothetical protein:cell division protein FtsA:->->:2037569..2037782 214 aaatgagctttctttttttcataaatctgattttttataacttgtttggaaaataaaaaaagctarggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcrcgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_2162:SMT_2163|SMT_2162:SMT_2163:hypothetical protein:cell division protein FtsA:->->:2037569..2037782 214 aaatgagctttctttttttcataaatctgattttttataacttgtttggaaaataaaaaaagctarggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcrcgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaatgagctttctttttttcataaatctgattttttataacttgtttggaaaataaaaaaagctarggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcrcgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta Predict ORF larger than 30AA ================================================ Protein_Len: 47 Strand: - Start: 66 End: 206 ................................................................. P F F T F F H Y T F K P F V L X S Q I K Q T I I N Q S Y S H G I K A I N F L L K F S P L S L M ........ aaatgagctttctttttttcataaatctgattttttataacttgtttggaaaataaaaaaagctarggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcrcgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 82 Start: 158 End: 187 .............................................................................................................................................................TTTCAAACTTCTATTCAATATAGCTAAAAT........................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaatgagctttctttttttcataaatctgattttttataacttgtttggaaaataaaaaaagctarggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcrcgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_2162:SMT_2163|SMT_2162:SMT_2163:hypothetical protein:cell division protein FtsA:->->:2037569..2037782 214 aaatgagctttctttttttcataaatctgattttttataacttgtttggaaaataaaaaaagctarggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcrcgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta Intra-Species Hit: Count: 7 Min: 20 Max: 214 Len: 195 Subject: smit_SMT_2162_SMT_2163|hypothetical protein:cell division protein FtsA|POSITIVE:POSITIVE|[2037569,2037782]|214 HSP 1 e-value: 7.0E-54 bit: 211.0 Len: 108 Query Start:107 Query End:214 Subject Strand: POSITIVE Subject Start: 107 Subject End: 214 ..........................................................................................................ctaagcrcgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta ..........................................................................................................ctaagcrcgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta HSP 2 e-value: 1.0E-7 bit: 58.2 Len: 29 Query Start:20 Query End:48 Subject Strand: POSITIVE Subject Start: 20 Subject End: 48 ...................cataaatctgattttttataacttgtttg...................................................................................................................................................................... ...................cataaatctgattttttataacttgtttg...................................................................................................................................................................... Subject: smit_SMT_2157_SMT_2158|hypothetical protein:cell division protein|POSITIVE:POSITIVE|[2031460,2031673]|214 HSP 1 e-value: 7.0E-54 bit: 211.0 Len: 108 Query Start:107 Query End:214 Subject Strand: POSITIVE Subject Start: 107 Subject End: 214 ..........................................................................................................ctaagcrcgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta ..........................................................................................................ctaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta HSP 2 e-value: 1.0E-7 bit: 58.2 Len: 29 Query Start:20 Query End:48 Subject Strand: POSITIVE Subject Start: 20 Subject End: 48 ...................cataaatctgattttttataacttgtttg...................................................................................................................................................................... ...................cataaatctgattttttataacttgtttg...................................................................................................................................................................... Subject: smit_SMT_1706_SMT_1707|hypothetical protein:cell division protein FtsA|POSITIVE:POSITIVE|[1690871,1691084]|214 HSP 1 e-value: 7.0E-54 bit: 211.0 Len: 108 Query Start:107 Query End:214 Subject Strand: POSITIVE Subject Start: 107 Subject End: 214 ..........................................................................................................ctaagcrcgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta ..........................................................................................................ctaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta HSP 2 e-value: 1.0E-7 bit: 58.2 Len: 29 Query Start:20 Query End:48 Subject Strand: POSITIVE Subject Start: 20 Subject End: 48 ...................cataaatctgattttttataacttgtttg...................................................................................................................................................................... ...................cataaatctgattttttataacttgtttg...................................................................................................................................................................... Subject: smit_SMT_1546_SMT_1547|hypothetical protein:cell division protein FtsA|POSITIVE:POSITIVE|[1530913,1531126]|214 HSP 1 e-value: 7.0E-54 bit: 211.0 Len: 108 Query Start:107 Query End:214 Subject Strand: POSITIVE Subject Start: 107 Subject End: 214 ..........................................................................................................ctaagcrcgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta ..........................................................................................................ctaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta HSP 2 e-value: 1.0E-7 bit: 58.2 Len: 29 Query Start:20 Query End:48 Subject Strand: POSITIVE Subject Start: 20 Subject End: 48 ...................cataaatctgattttttataacttgtttg...................................................................................................................................................................... ...................cataaatctgattttttataacttgtttg...................................................................................................................................................................... Subject: smit_SMT_2158_SMT_2159|cell division protein:cell division protein FtsA|POSITIVE:POSITIVE|[2031986,2032149]|164 HSP 1 e-value: 5.0E-49 bit: 195.0 Len: 108 Query Start:107 Query End:214 Subject Strand: POSITIVE Subject Start: 57 Subject End: 164 ..........................................................................................................ctaagcrcgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta ..........................................................................................................ctaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagcttgaaacttggaagagagtggatgcgatgta Subject: smit_SMT_2159_SMT_2160|cell division protein FtsA:cell division protein FtsA|POSITIVE:POSITIVE|[2033428,2033663]|236 HSP 1 e-value: 3.0E-47 bit: 189.0 Len: 108 Query Start:107 Query End:214 Subject Strand: POSITIVE Subject Start: 128 Subject End: 236 ..........................................................................................................ctaagcrcgattggatttt-ttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta ..........................................................................................................ctaagcacgattggatttttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgagacttggaagagagaggatgcgatgta Subject: smit_SMT_2167_SMT_2168|hypothetical protein:threonyl-tRNA synthetase|POSITIVE:POSITIVE|[2040772,2041130]|359 HSP 1 e-value: 7.0E-45 bit: 181.0 Len: 108 Query Start:107 Query End:214 Subject Strand: POSITIVE Subject Start: 107 Subject End: 215 ..........................................................................................................ctaagcrcgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaag-tttgaaacttggaagagagaggatgcgatgta ..........................................................................................................ctaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagttttgaactttggaagagagaggatgcgatgta HSP 2 e-value: 1.0E-7 bit: 58.2 Len: 29 Query Start:20 Query End:48 Subject Strand: POSITIVE Subject Start: 20 Subject End: 48 ...................cataaatctgattttttataacttgtttg...................................................................................................................................................................... ...................cataaatctgattttttataacttgtttg...................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAUAAAUCUGAUUUUUUAUAACUUGUUUGGAAAAUAAAAAAAGCUARGGAAAGAAUGUGAAAAAAUGGUAAGUAAAUUUAGGAAAAACUAAGCRCGAUUGGAUUUUUUGUGUUAUAAUAUUCUGUGAAUAGCUAUGCCCUAUUUUAGCUAUAUUGAAUAGAAGUUUGAAACUUGGAAGAGAGAGGAUGCGAUGUA (((..((((..((((((....(..((((.(((.........(((((.....(((((((.((.(((..((.(((...(((((......)))))....)))..))..)))...))...))))))).....))))).....((((((..........))))))...))).))))..).))))))..))))...))).. (-30.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACAUCGCAUCCUCUCUCUUCCAAGUUUCAAACUUCUAUUCAAUAUAGCUAAAAUAGGGCAUAGCUAUUCACAGAAUAUUAUAACACAAAAAAUCCAAUCGYGCUUAGUUUUUCCUAAAUUUACUUACCAUUUUUUCACAUUCUUUCCYUAGCUUUUUUUAUUUUCCAAACAAGUUAUAAAAAAUCAGAUUUAUG ...(((................((((.....))))........((((((((((((((((...(((((.....(((((..........(((((((..........((((......))))...........)))))))...))))).....)))))..))))))))).......)))))))........)))..... (-13.43) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 311 189 146 86 47 smit:SMT_112|5end_hypothetical_protein_predicted_by_Glimmer/Critica_61473..61703_NEGATIVE 233 175 132 138 92 smit:SMT_446|5end_ABC-type_cobalt_transport_system,_ATPase_component_384870..385100_POSITIVE 232 190 161 144 115 smit:SMT_1303|5end_tRNA-dihydrouridine_synthase_1265731..1265961_NEGATIVE 225 143 101 150 105 smit:SMT_1752|5end_N-(5'-phosphoribosyl)-anthranilate_isomerase_1729772..1730002_POSITIVE 220 140 95 227 178 smit:SMT_752|5end_prephenate_dehydrogenase_656791..657021_NEGATIVE 211 160 111 203 143 smit:SMT_889|5end_K02029_polar_amino_acid_transport_system_permease_protein_836266..836496_POSITIVE 204 190 145 211 165 smit:SMT_2134|5end_hypothetical_protein_2014700..2014930_NEGATIVE 204 190 145 211 165 smit:SMT_873|5end_hypothetical_protein_821311..821541_NEGATIVE 203 167 122 159 121 smit:SMT_1013|5end_galactose-1-phosphate_uridylyltransferase_950626..950856_NEGATIVE 202 180 131 205 156 smit:SMT_693|5end_hypothetical_protein_603819..604049_POSITIVE 199 168 121 132 78 smit:SMT_1833|5end_tagatose_1,6-diphosphate_aldolase_1801369..1801599_POSITIVE 197 174 132 147 106 smit:SMT_1954|5end_6-phospho-beta-glucosidase_1888548..1888778_POSITIVE 197 140 91 99 53 smit:SMT_1117|5end_competence_protein_CglA_1054703..1054933_NEGATIVE 195 150 120 117 69 smit:SMT_2127|5end_exodeoxyribonuclease_V_alpha_chain_2009306..2009536_POSITIVE 194 176 138 151 117 smit:SMT_1183|5end_K02795_PTS_system,_mannose-specific_IIC_component_1141534..1141764_NEGATIVE 193 179 131 147 87 smit:SMT_1891|5end_putative_dihydrolipoamide_S-acetyltransferase_1844373..1844603_POSITIVE 193 148 109 203 167 smit:SMT_1416|5end_hypothetical_protein_1400829..1401059_NEGATIVE 193 179 131 147 87 smit:SMT_1402|5end_pyruvate_dehydrogenase_E2_component_(dihydrolipoamide_acetyltransferase)_1378115..1378345_POSITIVE 192 148 108 127 79 smit:SMT_1572|5end_excinuclease_ABC_subunit_B_1564402..1564632_POSITIVE 191 179 138 231 187 smit:SMT_1619|5end_K02006_cobalt_transport_system_ATP-binding_protein_1606526..1606756_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 253 168 123 141 99 smit:SMT_306|3end_3-oxoacyl-_249117..249267_POSITIVE 233 175 132 137 91 smit:SMT_445|3end_hypothetical_protein_384949..385099_POSITIVE 221 168 123 133 96 smit:SMT_2004|3end_K00783_hypothetical_protein_1925489..1925639_POSITIVE 221 168 123 133 96 smit:SMT_86|3end_K00783_hypothetical_protein_17035..17185_NEGATIVE 220 180 138 63 18 smit:SMT_1620|3end_hypothetical_protein_1606695..1606845_NEGATIVE 203 167 122 140 102 smit:SMT_1014|3end_galactokinase_950645..950795_NEGATIVE 202 148 104 136 95 smit:SMT_686|3end_LPS_biosynthesis_protein_596048..596198_POSITIVE 202 176 135 148 113 smit:SMT_539|3end_sensor_histidine_kinase_CiaH_456059..456209_POSITIVE 200 181 145 145 111 smit:SMT_2135|3end_dipeptidase_2014754..2014904_NEGATIVE 200 181 145 145 111 smit:SMT_874|3end_dipeptidase_821365..821515_NEGATIVE 199 168 121 130 76 smit:SMT_1832|3end_tagatose-6-phosphate_kinase_1801447..1801597_POSITIVE 195 150 120 97 49 smit:SMT_2126|3end_indole-3-glycerol_phosphate_synthase_2009366..2009516_POSITIVE 195 187 145 47 4 smit:SMT_1773|3end_hypothetical_protein_1749905..1750055_POSITIVE 194 176 138 124 90 smit:SMT_1184|3end_phosphotransferase_system,_mannose/fructose/N-acetylgalactosamine-specific_component_IIB_1141561..1141711_NEGATIVE 192 148 108 50 2 smit:SMT_1571|3end_CAAX_amino_terminal_protease_family_protein_1564405..1564555_POSITIVE 187 110 61 86 33 smit:SMT_1004|3end_thioredoxin_945824..945974_POSITIVE 187 148 122 150 122 smit:SMT_313|3end_3R-hydroxymyristoyl_ACP_dehydrase_254345..254495_POSITIVE 186 189 166 129 106 smit:SMT_621|3end_K03346_replication_initiation_and_membrane_attachment_protein_529059..529209_POSITIVE 186 189 166 41 18 smit:SMT_620|3end_hypothetical_protein_predicted_by_Glimmer/Critica_528971..529121_POSITIVE 185 188 147 36 4 smit:SMT_1478|3end_manganese_transport_system_membrane_protein_1460408..1460558_NEGATIVE