Origin IGS: ttaannnnnaagaaagcttagaaatgagctttctttttttcatatatctgattttttataacttgtttggaaaataaaaaaagctaaggaaagaatgtgaaaaaacggtaagtaaatttaggaaaaactaagcacgattggatttttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgagacttggaagagagaggatgcgatgta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tacatcgcatcctctctcttccaagtctcaaacttctattcaatatagctaaaatagggcatagctattcacagaatattataacacaaaaaaatccaatcgtgcttagtttttcctaaatttacttaccgttttttcacattctttccttagctttttttattttccaaacaagttataaaaaatcagatatatgaaaaaaagaaagctcatttctaagctttcttnnnnnttaa Mask Tandem Repeat Region ================================================ ttaannnnnaagaaagcttagaaatgagctttctttttttcatatatctgattttttataacttgtttggaaaataaaaaaagctaaggaaagaatgtgaaaaaacggtaagtaaatttaggaaaaactaagcacgattggatttttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgagacttggaagagagaggatgcgatgta Find is-nt database================================================ Query_seq: SMT_2159:SMT_2160|SMT_2159:SMT_2160:cell division protein FtsA:cell division protein FtsA:->->:2033428..2033663 236 ttaannnnnaagaaagcttagaaatgagctttctttttttcatatatctgattttttataacttgtttggaaaataaaaaaagctaaggaaagaatgtgaaaaaacggtaagtaaatttaggaaaaactaagcacgattggatttttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgagacttggaagagagaggatgcgatgta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_2159:SMT_2160|SMT_2159:SMT_2160:cell division protein FtsA:cell division protein FtsA:->->:2033428..2033663 236 ttaannnnnaagaaagcttagaaatgagctttctttttttcatatatctgattttttataacttgtttggaaaataaaaaaagctaaggaaagaatgtgaaaaaacggtaagtaaatttaggaaaaactaagcacgattggatttttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgagacttggaagagagaggatgcgatgta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_2159:SMT_2160|SMT_2159:SMT_2160:cell division protein FtsA:cell division protein FtsA:->->:2033428..2033663 236 ttaannnnnaagaaagcttagaaatgagctttctttttttcatatatctgattttttataacttgtttggaaaataaaaaaagctaaggaaagaatgtgaaaaaacggtaagtaaatttaggaaaaactaagcacgattggatttttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgagacttggaagagagaggatgcgatgta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttaannnnnaagaaagcttagaaatgagctttctttttttcatatatctgattttttataacttgtttggaaaataaaaaaagctaaggaaagaatgtgaaaaaacggtaagtaaatttaggaaaaactaagcacgattggatttttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgagacttggaagagagaggatgcgatgta Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: - Start: 121 End: 228 ........................................................................................................................ S F S L V I P N K Q T I I N Q S Y S H G I K A I N F L L K L S P L S L M ........ ttaannnnnaagaaagcttagaaatgagctttctttttttcatatatctgattttttataacttgtttggaaaataaaaaaagctaaggaaagaatgtgaaaaaacggtaagtaaatttaggaaaaactaagcacgattggatttttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgagacttggaagagagaggatgcgatgta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttaannnnnaagaaagcttagaaatgagctttctttttttcatatatctgattttttataacttgtttggaaaataaaaaaagctaaggaaagaatgtgaaaaaacggtaagtaaatttaggaaaaactaagcacgattggatttttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgagacttggaagagagaggatgcgatgta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_2159:SMT_2160|SMT_2159:SMT_2160:cell division protein FtsA:cell division protein FtsA:->->:2033428..2033663 236 ttaannnnnaagaaagcttagaaatgagctttctttttttcatatatctgattttttataacttgtttggaaaataaaaaaagctaaggaaagaatgtgaaaaaacggtaagtaaatttaggaaaaactaagcacgattggatttttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgagacttggaagagagaggatgcgatgta Intra-Species Hit: Count: 1 Min: 10 Max: 236 Len: 227 Subject: smit_SMT_2159_SMT_2160|cell division protein FtsA:cell division protein FtsA|POSITIVE:POSITIVE|[2033428,2033663]|236 HSP 1 e-value: 9.0E-43 bit: 174.0 Len: 109 Query Start:128 Query End:236 Subject Strand: POSITIVE Subject Start: 128 Subject End: 236 ...............................................................................................................................ctaagcacgattggannnnnnngtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgagacttggaagagagaggatgcgatgta ...............................................................................................................................ctaagcacgattggatttttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgagacttggaagagagaggatgcgatgta HSP 2 e-value: 2.0E-13 bit: 77.8 Len: 60 Query Start:10 Query End:69 Subject Strand: POSITIVE Subject Start: 10 Subject End: 69 .........aagaaagcttagaaatgagctttcnnnnnnncatatatctgattttttataacttgtttg....................................................................................................................................................................... .........aagaaagcttagaaatgagctttctttttttcatatatctgattttttataacttgtttg....................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGAAAGCUUAGAAAUGAGCUUUCUUUUUUUCAUAUAUCUGAUUUUUUAUAACUUGUUUGGAAAAUAAAAAAAGCUAAGGAAAGAAUGUGAAAAAACGGUAAGUAAAUUUAGGAAAAACUAAGCACGAUUGGAUUUUUUUGUGUUAUAAUAUUCUGUGAAUAGCUAUGCCCUAUUUUAGCUAUAUUGAAUAGAAGUUUGAGACUUGGAAGAGAGAGGAUGCGAUGUA (((((((((((....))))))))))).....(((.(((((..((((((....(..((((.(((.(((((((((.((((........(((......))).........(((((......))))).....)))).))))))))).........((((((..(((((((..........))))))).....)))))).))).))))..).))))))..)))))..))).. (-46.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACAUCGCAUCCUCUCUCUUCCAAGUCUCAAACUUCUAUUCAAUAUAGCUAAAAUAGGGCAUAGCUAUUCACAGAAUAUUAUAACACAAAAAAAUCCAAUCGUGCUUAGUUUUUCCUAAAUUUACUUACCGUUUUUUCACAUUCUUUCCUUAGCUUUUUUUAUUUUCCAAACAAGUUAUAAAAAAUCAGAUAUAUGAAAAAAAGAAAGCUCAUUUCUAAGCUUUCUU ...........................(((....((((((...((((((((((((((((...(((((.....(((((.......(((..............))).((((......)))).....................))))).....)))))..))))))))).......)))))))...))).)))....)))....(((((((((........))))))))) (-26.85) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 279 219 178 84 47 smit:SMT_112|5end_hypothetical_protein_predicted_by_Glimmer/Critica_61473..61703_NEGATIVE 251 170 123 222 157 smit:SMT_959|5end_1-acyl-sn-glycerol-3-phosphate_acyltransferase_905283..905513_POSITIVE 239 216 193 140 115 smit:SMT_1303|5end_tRNA-dihydrouridine_synthase_1265731..1265961_NEGATIVE 234 203 162 224 184 smit:SMT_900|5end_5-methyltetrahydropteroyltriglutamate--_homocysteine_methyltransferase_850616..850846_NEGATIVE 233 207 164 138 92 smit:SMT_446|5end_ABC-type_cobalt_transport_system,_ATPase_component_384870..385100_POSITIVE 232 103 61 158 108 smit:SMT_947|5end_ferredoxin_896405..896635_POSITIVE 219 219 171 175 120 smit:SMT_2053|5end_hypothetical_membrane_associated_protein_1964422..1964652_NEGATIVE 217 50 5 91 33 smit:SMT_1838|5end_response_regulator_1805400..1805630_NEGATIVE 212 155 121 64 28 smit:SMT_1253|5end_beta-galactosidase_1220147..1220377_NEGATIVE 211 207 163 139 94 smit:SMT_986|5end_threonyl-tRNA_synthetase_931092..931322_POSITIVE 211 207 163 139 94 smit:SMT_925|5end_DNA-directed_RNA_polymerase_omega_chain_880283..880513_NEGATIVE 210 207 162 174 131 smit:SMT_573|5end_1-phosphofructokinase_490976..491206_POSITIVE 208 200 152 132 77 smit:SMT_1833|5end_tagatose_1,6-diphosphate_aldolase_1801369..1801599_POSITIVE 206 48 1 62 4 smit:SMT_828|5end_surface_protein_Rib_760767..760997_NEGATIVE 205 170 124 209 176 smit:SMT_1366|5end_K02238_competence_protein_ComEC_1346888..1347118_NEGATIVE 204 218 178 53 14 smit:SMT_2035|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1947167..1947397_POSITIVE 204 199 152 215 153 smit:SMT_889|5end_K02029_polar_amino_acid_transport_system_permease_protein_836266..836496_POSITIVE 203 187 141 119 69 smit:SMT_1944|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1879494..1879724_NEGATIVE 203 48 8 40 1 smit:SMT_1104|5end_K06346_spoIIIJ-associated_protein_1046784..1047014_NEGATIVE 203 199 154 159 121 smit:SMT_1013|5end_galactose-1-phosphate_uridylyltransferase_950626..950856_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 262 47 1 125 77 smit:SMT_2162|3end_hypothetical_protein_2037508..2037658_POSITIVE 262 47 1 125 77 smit:SMT_2157|3end_hypothetical_protein_2031399..2031549_POSITIVE 262 47 1 125 77 smit:SMT_1706|3end_hypothetical_protein_1690810..1690960_POSITIVE 262 47 1 125 77 smit:SMT_1546|3end_hypothetical_protein_1530852..1531002_POSITIVE 253 200 155 141 99 smit:SMT_306|3end_3-oxoacyl-_249117..249267_POSITIVE 251 170 123 98 33 smit:SMT_958|3end_hypothetical_protein_predicted_by_Glimmer/Critica_905239..905389_POSITIVE 247 139 97 90 48 smit:SMT_1074|3end_catabolite_control_protein_A_1012275..1012425_NEGATIVE 246 211 173 44 9 smit:SMT_1903|3end_hypothetical_protein_1853195..1853345_POSITIVE 244 47 1 125 77 smit:SMT_2167|3end_hypothetical_protein_2040711..2040861_POSITIVE 240 170 123 109 44 smit:SMT_2024|3end_hypothetical_protein_1940690..1940840_POSITIVE 240 170 123 98 33 smit:SMT_2002|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1924145..1924295_NEGATIVE 240 170 123 109 44 smit:SMT_1981|3end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1912868..1913018_POSITIVE 240 170 123 114 49 smit:SMT_1971|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1903526..1903676_POSITIVE 240 170 123 98 33 smit:SMT_1966|3end_DNA_polymerase_III_alpha_subunit_1898328..1898478_POSITIVE 240 170 123 109 44 smit:SMT_1942|3end_zinc_metalloprotease_ZmpB,_truncated_1878384..1878534_POSITIVE 240 170 123 98 33 smit:SMT_1927|3end_cell_division_protein_FtsH_1868408..1868558_NEGATIVE 240 170 123 109 44 smit:SMT_1926|3end_mevalonate_kinase_1868062..1868212_POSITIVE 240 170 123 109 44 smit:SMT_1887|3end_LicC_protein_1841740..1841890_POSITIVE 240 170 123 98 33 smit:SMT_1885|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1839830..1839980_NEGATIVE 240 170 123 98 33 smit:SMT_1873|3end_K02006_cobalt_transport_system_ATP-binding_protein_1832351..1832501_POSITIVE