Origin IGS: nnnnnaaaaaagctaaggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagcttgaaacttggaagagagtggatgcgatgta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tacatcgcatccactctcttccaagtttcaagcttctattcaatatagctaaaatagggcatagctattcacagaatattataacacaaaaaatccaatcgtgcttagtttttcctaaatttacttaccattttttcacattctttccttagcttttttnnnnn Mask Tandem Repeat Region ================================================ nnnnnaaaaaagctaaggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagcttgaaacttggaagagagtggatgcgatgta Find is-nt database================================================ Query_seq: SMT_2158:SMT_2159|SMT_2158:SMT_2159:cell division protein:cell division protein FtsA:->->:2031986..2032149 164 nnnnnaaaaaagctaaggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagcttgaaacttggaagagagtggatgcgatgta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_2158:SMT_2159|SMT_2158:SMT_2159:cell division protein:cell division protein FtsA:->->:2031986..2032149 164 nnnnnaaaaaagctaaggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagcttgaaacttggaagagagtggatgcgatgta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_2158:SMT_2159|SMT_2158:SMT_2159:cell division protein:cell division protein FtsA:->->:2031986..2032149 164 nnnnnaaaaaagctaaggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagcttgaaacttggaagagagtggatgcgatgta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 nnnnnaaaaaagctaaggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagcttgaaacttggaagagagtggatgcgatgta Predict ORF larger than 30AA ================================================ Protein_Len: 47 Strand: - Start: 16 End: 156 ............... P F F T F F H Y T F K P F V L C S Q I K Q T I I N Q S Y S H G I K A I N F L L K F S P L S H M ........ nnnnnaaaaaagctaaggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagcttgaaacttggaagagagtggatgcgatgta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ nnnnnaaaaaagctaaggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagcttgaaacttggaagagagtggatgcgatgta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_2158:SMT_2159|SMT_2158:SMT_2159:cell division protein:cell division protein FtsA:->->:2031986..2032149 164 nnnnnaaaaaagctaaggaaagaatgtgaaaaaatggtaagtaaatttaggaaaaactaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagcttgaaacttggaagagagtggatgcgatgta Intra-Species Hit: Count: 7 Min: 57 Max: 164 Len: 108 Subject: smit_SMT_2158_SMT_2159|cell division protein:cell division protein FtsA|POSITIVE:POSITIVE|[2031986,2032149]|164 HSP 1 e-value: 7.0E-55 bit: 214.0 Len: 108 Query Start:57 Query End:164 Subject Strand: POSITIVE Subject Start: 57 Subject End: 164 ........................................................ctaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagcttgaaacttggaagagagtggatgcgatgta ........................................................ctaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagcttgaaacttggaagagagtggatgcgatgta Subject: smit_SMT_2157_SMT_2158|hypothetical protein:cell division protein|POSITIVE:POSITIVE|[2031460,2031673]|214 HSP 1 e-value: 4.0E-50 bit: 198.0 Len: 108 Query Start:57 Query End:164 Subject Strand: POSITIVE Subject Start: 107 Subject End: 214 ........................................................ctaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagcttgaaacttggaagagagtggatgcgatgta ........................................................ctaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta Subject: smit_SMT_1706_SMT_1707|hypothetical protein:cell division protein FtsA|POSITIVE:POSITIVE|[1690871,1691084]|214 HSP 1 e-value: 4.0E-50 bit: 198.0 Len: 108 Query Start:57 Query End:164 Subject Strand: POSITIVE Subject Start: 107 Subject End: 214 ........................................................ctaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagcttgaaacttggaagagagtggatgcgatgta ........................................................ctaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta Subject: smit_SMT_1546_SMT_1547|hypothetical protein:cell division protein FtsA|POSITIVE:POSITIVE|[1530913,1531126]|214 HSP 1 e-value: 4.0E-50 bit: 198.0 Len: 108 Query Start:57 Query End:164 Subject Strand: POSITIVE Subject Start: 107 Subject End: 214 ........................................................ctaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagcttgaaacttggaagagagtggatgcgatgta ........................................................ctaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta Subject: smit_SMT_2162_SMT_2163|hypothetical protein:cell division protein FtsA|POSITIVE:POSITIVE|[2037569,2037782]|214 HSP 1 e-value: 6.0E-49 bit: 194.0 Len: 108 Query Start:57 Query End:164 Subject Strand: POSITIVE Subject Start: 107 Subject End: 214 ........................................................ctaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagcttgaaacttggaagagagtggatgcgatgta ........................................................ctaagcrcgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgaaacttggaagagagaggatgcgatgta Subject: smit_SMT_2159_SMT_2160|cell division protein FtsA:cell division protein FtsA|POSITIVE:POSITIVE|[2033428,2033663]|236 HSP 1 e-value: 1.0E-43 bit: 176.0 Len: 108 Query Start:57 Query End:164 Subject Strand: POSITIVE Subject Start: 128 Subject End: 236 ........................................................ctaagcacgattggatttt-ttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagcttgaaacttggaagagagtggatgcgatgta ........................................................ctaagcacgattggatttttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagtttgagacttggaagagagaggatgcgatgta Subject: smit_SMT_2167_SMT_2168|hypothetical protein:threonyl-tRNA synthetase|POSITIVE:POSITIVE|[2040772,2041130]|359 HSP 1 e-value: 4.0E-41 bit: 168.0 Len: 108 Query Start:57 Query End:164 Subject Strand: POSITIVE Subject Start: 107 Subject End: 215 ........................................................ctaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaag-cttgaaacttggaagagagtggatgcgatgta ........................................................ctaagcacgattggattttttgtgttataatattctgtgaatagctatgccctattttagctatattgaatagaagttttgaactttggaagagagaggatgcgatgta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUAAGCACGAUUGGAUUUUUUGUGUUAUAAUAUUCUGUGAAUAGCUAUGCCCUAUUUUAGCUAUAUUGAAUAGAAGCUUGAAACUUGGAAGAGAGUGGAUGCGAUGUA ....((((.(((...((((..(.(((.(((..((((((..(((((((..........))))))).....))))))..))).))).)..)))).))).).)))...... (-19.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACAUCGCAUCCACUCUCUUCCAAGUUUCAAGCUUCUAUUCAAUAUAGCUAAAAUAGGGCAUAGCUAUUCACAGAAUAUUAUAACACAAAAAAUCCAAUCGUGCUUAG ......((((............((((.....)))).(((((...(((((((..........))))))).....)))))......................)))).... (-11.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 250 100 52 175 120 smit:SMT_2053|5end_hypothetical_membrane_associated_protein_1964422..1964652_NEGATIVE 234 80 35 159 121 smit:SMT_1013|5end_galactose-1-phosphate_uridylyltransferase_950626..950856_NEGATIVE 231 94 58 199 165 smit:SMT_2134|5end_hypothetical_protein_2014700..2014930_NEGATIVE 231 94 58 199 165 smit:SMT_873|5end_hypothetical_protein_821311..821541_NEGATIVE 225 56 14 150 105 smit:SMT_1752|5end_N-(5'-phosphoribosyl)-anthranilate_isomerase_1729772..1730002_POSITIVE 225 89 41 164 120 smit:SMT_122|5end_hypothetical_protein_74456..74686_POSITIVE 224 100 59 84 47 smit:SMT_112|5end_hypothetical_protein_predicted_by_Glimmer/Critica_61473..61703_NEGATIVE 223 80 33 131 77 smit:SMT_1833|5end_tagatose_1,6-diphosphate_aldolase_1801369..1801599_POSITIVE 217 87 41 118 74 smit:SMT_1893|5end_ABC_transporter_substrate-binding_protein_-_sugar_transport_1846311..1846541_POSITIVE 215 53 13 227 181 smit:SMT_752|5end_prephenate_dehydrogenase_656791..657021_NEGATIVE 213 67 21 132 79 smit:SMT_1572|5end_excinuclease_ABC_subunit_B_1564402..1564632_POSITIVE 208 100 60 71 36 smit:SMT_1477|5end_K02077_zinc/manganese_transport_system_substrate-binding_protein_1460376..1460606_NEGATIVE 207 98 66 54 28 smit:SMT_1242|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1203383..1203613_NEGATIVE 205 81 43 221 184 smit:SMT_900|5end_5-methyltetrahydropteroyltriglutamate--_homocysteine_methyltransferase_850616..850846_NEGATIVE 203 68 22 119 69 smit:SMT_1944|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1879494..1879724_NEGATIVE 200 77 33 230 186 smit:SMT_451|5end_thiamine-phosphate_pyrophosphorylase_388935..389165_POSITIVE 198 60 11 108 62 smit:SMT_1091|5end_competence-specific_global_transcription_modulator_1033040..1033270_NEGATIVE 198 100 57 74 18 smit:SMT_326|5end_K02029_polar_amino_acid_transport_system_permease_protein_263864..264094_NEGATIVE 197 88 45 154 106 smit:SMT_1954|5end_6-phospho-beta-glucosidase_1888548..1888778_POSITIVE 197 99 51 185 126 smit:SMT_559|5end_D-alanyl-D-alanine_carboxypeptidase_478042..478272_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 267 80 36 140 99 smit:SMT_306|3end_3-oxoacyl-_249117..249267_POSITIVE 244 98 63 128 98 smit:SMT_717|3end_Mn2+_and_Fe2+_transporter_of_the_NRAMP_family_622815..622965_NEGATIVE 243 98 51 68 18 smit:SMT_1620|3end_hypothetical_protein_1606695..1606845_NEGATIVE 240 80 31 132 92 smit:SMT_2004|3end_K00783_hypothetical_protein_1925489..1925639_POSITIVE 240 80 31 132 92 smit:SMT_86|3end_K00783_hypothetical_protein_17035..17185_NEGATIVE 234 80 35 140 102 smit:SMT_1014|3end_galactokinase_950645..950795_NEGATIVE 233 89 48 148 113 smit:SMT_539|3end_sensor_histidine_kinase_CiaH_456059..456209_POSITIVE 231 94 58 145 111 smit:SMT_2135|3end_dipeptidase_2014754..2014904_NEGATIVE 231 94 58 145 111 smit:SMT_874|3end_dipeptidase_821365..821515_NEGATIVE 223 80 33 129 75 smit:SMT_1832|3end_tagatose-6-phosphate_kinase_1801447..1801597_POSITIVE 213 67 21 55 2 smit:SMT_1571|3end_CAAX_amino_terminal_protease_family_protein_1564405..1564555_POSITIVE 208 100 60 39 4 smit:SMT_1478|3end_manganese_transport_system_membrane_protein_1460408..1460558_NEGATIVE 202 79 41 125 87 smit:SMT_1927|3end_cell_division_protein_FtsH_1868408..1868558_NEGATIVE 201 100 59 57 15 smit:SMT_88|3end_K03497_chromosome_partitioning_protein,_ParB_family_19724..19874_POSITIVE 198 89 46 42 2 smit:SMT_121|3end_hypothetical_protein_74414..74564_POSITIVE 197 100 65 46 9 smit:SMT_427|3end_hypothetical_protein_predicted_by_Glimmer/Critica_369119..369269_POSITIVE 195 63 33 97 49 smit:SMT_2126|3end_indole-3-glycerol_phosphate_synthase_2009366..2009516_POSITIVE 194 59 11 53 7 smit:SMT_645|3end_hypothetical_protein_550019..550169_NEGATIVE 194 100 51 139 85 smit:SMT_455|3end_cation_transport_ATPase_393993..394143_POSITIVE 193 100 52 140 81 smit:SMT_1725|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1711501..1711651_POSITIVE