Origin IGS: gaaaagatagagagccccaaataaaaatcgtacatttccannnnnttaattaattaannnnntgttagaaagtttagaaaatgaagcaaagtgattttgtcaaacatttacgtattttttaatccatttttgacaaatagcatggtataataatatgcaggaaaattttgaatcatgaggaagactag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctagtcttcctcatgattcaaaattttcctgcatattattataccatgctatttgtcaaaaatggattaaaaaatacgtaaatgtttgacaaaatcactttgcttcattttctaaactttctaacannnnnttaattaattaannnnntggaaatgtacgatttttatttggggctctctatcttttc Mask Tandem Repeat Region ================================================ gaaaagatagagagccccaaataaaaatcgtacatttccannnnnttaattaattaannnnntgttagaaagtttagaaaatgaagcaaagtgattttgtcaaacatttacgtattttttaatccatttttgacaaatagcatggtataataatatgcaggaaaattttgaatcatgaggaagactag Find is-nt database================================================ Query_seq: SMT_2156:SMT_2157|SMT_2156:SMT_2157:hypothetical protein predicted by Glimmer/Critica:hypothetical protein:<-->:2030567..2030754 188 gaaaagatagagagccccaaataaaaatcgtacatttccannnnnttaattaattaannnnntgttagaaagtttagaaaatgaagcaaagtgattttgtcaaacatttacgtattttttaatccatttttgacaaatagcatggtataataatatgcaggaaaattttgaatcatgaggaagactag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_2156:SMT_2157|SMT_2156:SMT_2157:hypothetical protein predicted by Glimmer/Critica:hypothetical protein:<-->:2030567..2030754 188 gaaaagatagagagccccaaataaaaatcgtacatttccannnnnttaattaattaannnnntgttagaaagtttagaaaatgaagcaaagtgattttgtcaaacatttacgtattttttaatccatttttgacaaatagcatggtataataatatgcaggaaaattttgaatcatgaggaagactag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_2156:SMT_2157|SMT_2156:SMT_2157:hypothetical protein predicted by Glimmer/Critica:hypothetical protein:<-->:2030567..2030754 188 gaaaagatagagagccccaaataaaaatcgtacatttccannnnnttaattaattaannnnntgttagaaagtttagaaaatgaagcaaagtgattttgtcaaacatttacgtattttttaatccatttttgacaaatagcatggtataataatatgcaggaaaattttgaatcatgaggaagactag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gaaaagatagagagccccaaataaaaatcgtacatttccannnnnttaattaattaannnnntgttagaaagtttagaaaatgaagcaaagtgattttgtcaaacatttacgtattttttaatccatttttgacaaatagcatggtataataatatgcaggaaaattttgaatcatgaggaagactag Predict ORF larger than 30AA ================================================ gaaaagatagagagccccaaataaaaatcgtacatttccannnnnttaattaattaannnnntgttagaaagtttagaaaatgaagcaaagtgattttgtcaaacatttacgtattttttaatccatttttgacaaatagcatggtataataatatgcaggaaaattttgaatcatgaggaagactag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gaaaagatagagagccccaaataaaaatcgtacatttccannnnnttaattaattaannnnntgttagaaagtttagaaaatgaagcaaagtgattttgtcaaacatttacgtattttttaatccatttttgacaaatagcatggtataataatatgcaggaaaattttgaatcatgaggaagactag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_2156:SMT_2157|SMT_2156:SMT_2157:hypothetical protein predicted by Glimmer/Critica:hypothetical protein:<-->:2030567..2030754 188 gaaaagatagagagccccaaataaaaatcgtacatttccannnnnttaattaattaannnnntgttagaaagtttagaaaatgaagcaaagtgattttgtcaaacatttacgtattttttaatccatttttgacaaatagcatggtataataatatgcaggaaaattttgaatcatgaggaagactag Intra-Species Hit: Count: 1 Min: 1 Max: 188 Len: 188 Subject: smit_SMT_2156_SMT_2157|hypothetical protein predicted by Glimmer/Critica:hypothetical protein|NEGATIVE:POSITIVE|[2030567,2030754]|188 HSP 1 e-value: 1.0E-84 bit: 313.0 Len: 188 Query Start:1 Query End:188 Subject Strand: POSITIVE Subject Start: 1 Subject End: 188 gaaaagatagagagccccaaataaaaatcgtacatttccannnnnttaattaattaannnnntgttagaaagtttagaaaatgaagcaaagtgattttgtcaaacatttacgtattttttaatccatttttgacaaatagcatggtataataatatgcaggaaaattttgaatcatgaggaagactag gaaaagatagagagccccaaataaaaatcgtacatttccannnnnttaattaattaannnnntgttagaaagtttagaaaatgaagcaaagtgattttgtcaaacatttacgtattttttaatccatttttgacaaatagcatggtataataatatgcaggaaaattttgaatcatgaggaagactag Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAAAAGAUAGAGAGCCCCAAAUAAAAAUCGUACAUUUCCANNNNNUUAAUUAAUUAANNNNNUGUUAGAAAGUUUAGAAAAUGAAGCAAAGUGAUUUUGUCAAACAUUUACGUAUUUUUUAAUCCAUUUUUGACAAAUAGCAUGGUAUAAUAAUAUGCAGGAAAAUUUUGAAUCAUGAGGAAGACUAG .......(((....((.((........(((....(((((.......................(((((((((..((((((((((.......((((...((.....)).)))).))))))))))....)))))))))....(((((.........))))).)))))....)))....)).))....))). (-24.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUAGUCUUCCUCAUGAUUCAAAAUUUUCCUGCAUAUUAUUAUACCAUGCUAUUUGUCAAAAAUGGAUUAAAAAAUACGUAAAUGUUUGACAAAAUCACUUUGCUUCAUUUUCUAAACUUUCUAACANNNNNUUAAUUAAUUAANNNNNUGGAAAUGUACGAUUUUUAUUUGGGGCUCUCUAUCUUUUC .(((....(((((.((..((((........((((...........))))..(((((((((.(((.(((....))).))).....))))))))).....))))..)).........((((((((........((((....)))).....)))))).))............)))))....)))....... (-20.32) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 768 180 131 174 125 smit:SMT_1545|5end_choline-binding_protein_1530026..1530256_NEGATIVE 682 130 81 221 172 smit:SMT_2156|5end_hypothetical_protein_predicted_by_Glimmer/Critica_2030476..2030706_NEGATIVE 627 40 1 131 92 smit:SMT_2175|5end_hypothetical_protein_2045727..2045957_NEGATIVE 627 40 1 152 113 smit:SMT_2148|5end_hypothetical_protein_2023635..2023865_NEGATIVE 627 40 1 152 113 smit:SMT_2147|5end_hypothetical_protein_2022895..2023125_NEGATIVE 610 39 1 130 92 smit:SMT_2170|5end_hypothetical_protein_2042242..2042472_NEGATIVE 610 39 1 151 113 smit:SMT_2151|5end_hypothetical_protein_2025520..2025750_NEGATIVE 426 57 15 196 154 smit:SMT_2168|5end_threonyl-tRNA_synthetase_2040991..2041221_POSITIVE 426 57 15 113 71 smit:SMT_2150|5end_threonyl-tRNA_synthetase_2024673..2024903_POSITIVE 426 57 15 113 71 smit:SMT_2146|5end_threonyl-tRNA_synthetase_2022048..2022278_POSITIVE 248 40 2 100 50 smit:SMT_879|5end_hypothetical_protein_826989..827219_NEGATIVE 238 164 121 65 14 smit:SMT_717|5end_Mn2+_and_Fe2+_transporter_of_the_NRAMP_family_623147..623377_NEGATIVE 218 100 82 173 155 smit:SMT_335|5end_glutamine_synthetase_273753..273983_POSITIVE 211 130 85 190 147 smit:SMT_1255|5end_cell_division_protein_FtsH_1221811..1222041_NEGATIVE 211 164 121 228 173 smit:SMT_81|5end_K01421_putative_membrane_protein_13630..13860_NEGATIVE 210 146 111 78 50 smit:SMT_825|5end_aspartate_aminotransferase_749594..749824_NEGATIVE 209 57 13 149 103 smit:SMT_1681|5end_D-alanine-activating_enzyme_1664184..1664414_POSITIVE 202 38 3 89 49 smit:SMT_2053|5end_hypothetical_membrane_associated_protein_1964422..1964652_NEGATIVE 193 158 123 54 11 smit:SMT_187|5end_30S_ribosomal_protein_S5_122997..123227_POSITIVE 189 163 125 46 10 smit:SMT_1527|5end_two-component_sensor_kinase_1511183..1511413_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 627 40 1 85 46 smit:SMT_73|3end_peptidase,_M16_family_-84..66_NEGATIVE 548 110 65 74 29 smit:SMT_2156|3end_hypothetical_protein_predicted_by_Glimmer/Critica_2030297..2030447_NEGATIVE 426 57 15 94 52 smit:SMT_2149|3end_UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-_diaminopimelate--D-alanyl-D-alanine_ligase_2024734..2024884_POSITIVE 218 100 82 136 118 smit:SMT_334|3end_K03713_MerR_family_transcriptional_regulator,_glutamine_synthetase_repressor_273796..273946_POSITIVE 211 130 85 146 103 smit:SMT_1256|3end_ketol-acid_reductoisomerase_1221855..1222005_NEGATIVE 210 146 111 81 53 smit:SMT_826|3end_hypothetical_protein_749591..749741_NEGATIVE 209 57 13 58 12 smit:SMT_1680|3end_hypothetical_protein_1664173..1664323_POSITIVE 187 40 6 97 69 smit:SMT_1273|3end_K02006_cobalt_transport_system_ATP-binding_protein_1237828..1237978_POSITIVE 187 112 79 46 19 smit:SMT_933|3end_ATP-dependent_RNA_helicase_885365..885515_NEGATIVE 186 126 86 151 107 smit:SMT_644|3end_O-acetylhomoserine_(thiol)-lyase_548591..548741_NEGATIVE 184 39 15 22 1 smit:SMT_1574|3end_K02529_LacI_family_transcriptional_regulator_1568144..1568294_POSITIVE 182 130 90 97 57 smit:SMT_356|3end_hypothetical_protein_293268..293418_POSITIVE 179 164 137 50 19 smit:SMT_148|3end_glycoprotein_endopeptidase_98601..98751_POSITIVE 174 141 111 85 51 smit:SMT_1157|3end_septation_ring_formation_regulator_EzrA_1100202..1100352_NEGATIVE 171 127 82 69 28 smit:SMT_319|3end_K02356_elongation_factor_EF-P_258304..258454_NEGATIVE 168 30 3 48 19 smit:SMT_1279|3end_translation_elongation_factor_Ts_1243451..1243601_POSITIVE 166 57 14 94 41 smit:SMT_1862|3end_K02027_multiple_sugar_transport_system_substrate-binding_protein_1825460..1825610_POSITIVE 164 40 8 76 43 smit:SMT_403|3end_two-component_sensor_kinase_YesM_333351..333501_POSITIVE 164 180 131 75 22 smit:SMT_135|3end_hypothetical_protein_86178..86328_NEGATIVE 161 158 120 147 111 smit:SMT_1248|3end_Na+/H+_antiporter_1208667..1208817_NEGATIVE