Origin IGS: gttttcaaggattgacttgatttgctctgggcttgttccctttggatagcggatatnnnnnttaattaattaannnnnatattcttcaagaaggtggagattatgtcacaaaagatatctctacagtc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gactgtagagatatcttttgtgacataatctccaccttcttgaagaatatnnnnnttaattaattaannnnnatatccgctatccaaagggaacaagcccagagcaaatcaagtcaatccttgaaaac Mask Tandem Repeat Region ================================================ gttttcaaggattgacttgatttgctctgggcttgttccctttggatagcggatatnnnnnttaattaattaannnnnatattcttcaagaaggtggagattatgtcacaaaagatatctctacagtc Find is-nt database================================================ Query_seq: SMT_2135:SMT_2136|SMT_2135:SMT_2136:dipeptidase:cell division protein FtsA:<-->:2015144..2015271 128 gttttcaaggattgacttgatttgctctgggcttgttccctttggatagcggatatnnnnnttaattaattaannnnnatattcttcaagaaggtggagattatgtcacaaaagatatctctacagtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_2135:SMT_2136|SMT_2135:SMT_2136:dipeptidase:cell division protein FtsA:<-->:2015144..2015271 128 gttttcaaggattgacttgatttgctctgggcttgttccctttggatagcggatatnnnnnttaattaattaannnnnatattcttcaagaaggtggagattatgtcacaaaagatatctctacagtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_2135:SMT_2136|SMT_2135:SMT_2136:dipeptidase:cell division protein FtsA:<-->:2015144..2015271 128 gttttcaaggattgacttgatttgctctgggcttgttccctttggatagcggatatnnnnnttaattaattaannnnnatattcttcaagaaggtggagattatgtcacaaaagatatctctacagtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gttttcaaggattgacttgatttgctctgggcttgttccctttggatagcggatatnnnnnttaattaattaannnnnatattcttcaagaaggtggagattatgtcacaaaagatatctctacagtc Predict ORF larger than 30AA ================================================ gttttcaaggattgacttgatttgctctgggcttgttccctttggatagcggatatnnnnnttaattaattaannnnnatattcttcaagaaggtggagattatgtcacaaaagatatctctacagtc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gttttcaaggattgacttgatttgctctgggcttgttccctttggatagcggatatnnnnnttaattaattaannnnnatattcttcaagaaggtggagattatgtcacaaaagatatctctacagtc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_2135:SMT_2136|SMT_2135:SMT_2136:dipeptidase:cell division protein FtsA:<-->:2015144..2015271 128 gttttcaaggattgacttgatttgctctgggcttgttccctttggatagcggatatnnnnnttaattaattaannnnnatattcttcaagaaggtggagattatgtcacaaaagatatctctacagtc Intra-Species Hit: Count: 1 Min: 1 Max: 128 Len: 128 Subject: smit_SMT_2135_SMT_2136|dipeptidase:cell division protein FtsA|NEGATIVE:POSITIVE|[2015144,2015271]|128 HSP 1 e-value: 5.0E-49 bit: 194.0 Len: 128 Query Start:1 Query End:128 Subject Strand: POSITIVE Subject Start: 1 Subject End: 128 gttttcaaggattgacttgatttgctctgggcttgttccctttggatagcggatatnnnnnttaattaattaannnnnatattcttcaagaaggtggagattatgtcacaaaagatatctctacagtc gttttcaaggattgacttgatttgctctgggcttgttccctttggatagcggatatnnnnnttaattaattaannnnnatattcttcaagaaggtggagattatgtcacaaaagatatctctacagtc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUUUCAAGGAUUGACUUGAUUUGCUCUGGGCUUGUUCCCUUUGGAUAGCGGAUAUNNNNNUUAAUUAAUUAANNNNNAUAUUCUUCAAGAAGGUGGAGAUUAUGUCACAAAAGAUAUCUCUACAGUC ((..(((((......)))))...)).....(((...(((....))).)))((((((.....((((....)))).....)))))).....((..((((((((...(((......)))))))))))..)) (-24.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GACUGUAGAGAUAUCUUUUGUGACAUAAUCUCCACCUUCUUGAAGAAUAUNNNNNUUAAUUAAUUAANNNNNAUAUCCGCUAUCCAAAGGGAACAAGCCCAGAGCAAAUCAAGUCAAUCCUUGAAAAC ((..((.(((((.((......))....))))).))..)).......................................(((.......(((......)))..)))...(((((......))))).... (-14.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 908 50 1 141 92 smit:SMT_2135|5end_dipeptidase_2015053..2015283_NEGATIVE 338 50 2 136 85 smit:SMT_791|5end_thiamine_biosynthesis_protein_ThiI_702377..702607_NEGATIVE 328 46 5 64 24 smit:SMT_1854|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1818375..1818605_POSITIVE 316 47 1 60 3 smit:SMT_1824|5end_hypothetical_protein_1796126..1796356_POSITIVE 286 47 7 100 52 smit:SMT_2155|5end_UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-_diaminopimelate--D-alanyl-D-alanine_ligase_2028863..2029093_POSITIVE 285 44 1 104 64 smit:SMT_1781|5end_transcriptional_regulator,_xre_family_1758365..1758595_POSITIVE 279 51 12 208 168 smit:SMT_1456|5end_pyrimidine-nucleoside_phosphorylase_1439832..1440062_NEGATIVE 277 45 1 207 153 smit:SMT_1769|5end_alcohol_dehydrogenase,_zinc-containing_1745977..1746207_NEGATIVE 275 48 2 80 27 smit:SMT_982|5end_D-tyrosyl-tRNA(Tyr)_deacylase_929654..929884_NEGATIVE 269 40 3 143 96 smit:SMT_1897|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1848858..1849088_NEGATIVE 267 52 12 187 151 smit:SMT_825|5end_aspartate_aminotransferase_749594..749824_NEGATIVE 267 49 3 63 11 smit:SMT_366|5end_hypothetical_protein_302085..302315_POSITIVE 266 49 5 175 117 smit:SMT_784|5end_hypothetical_protein_696298..696528_NEGATIVE 257 41 1 74 27 smit:SMT_1124|5end_pyrrolidone-carboxylate_peptidase_1060869..1061099_NEGATIVE 255 52 11 175 143 smit:SMT_2005|5end_pullulanase,_type_I_1926289..1926519_NEGATIVE 254 52 15 214 167 smit:SMT_142|5end_hypothetical_protein_95088..95318_NEGATIVE 253 48 4 226 193 smit:SMT_380|5end_hypothetical_protein_316376..316606_POSITIVE 253 48 4 226 193 smit:SMT_371|5end_hypothetical_protein_305248..305478_POSITIVE 251 49 1 117 61 smit:SMT_1818|5end_tryptophan_synthase,_alpha_subunit_1792190..1792420_POSITIVE 251 44 1 95 61 smit:SMT_723|5end_hypothetical_protein_629475..629705_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 338 50 2 55 4 smit:SMT_792|3end_hypothetical_protein_predicted_by_Glimmer/Critica_702458..702608_NEGATIVE 289 52 11 54 15 smit:SMT_780|3end_peptidase_family_U32_688752..688902_NEGATIVE 286 47 7 49 1 smit:SMT_2154|3end_UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-_diaminopimelate--D-alanyl-D-alanine_ligase_2028892..2029042_POSITIVE 277 45 1 110 56 smit:SMT_1770|3end_hypothetical_protein_1746074..1746224_NEGATIVE 269 40 3 124 77 smit:SMT_1898|3end_aminotransferase_(class_V),_putative_1848877..1849027_NEGATIVE 257 48 9 50 4 smit:SMT_983|3end_GTP_pyrophosphokinase_929684..929834_NEGATIVE 253 107 90 88 71 smit:SMT_1911|3end_hypothetical_protein_1857840..1857990_POSITIVE 253 107 90 88 71 smit:SMT_867|3end_hypothetical_protein_816542..816692_POSITIVE 248 47 7 57 9 smit:SMT_2153|3end_putative_cell_division_protein_2027512..2027662_POSITIVE 248 47 7 69 21 smit:SMT_2139|3end_cell_division_protein_FtsZ_2017690..2017840_POSITIVE 248 47 7 67 19 smit:SMT_2131|3end_UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-_diaminopimelate--D-alanyl-D-alanine_ligase_2012731..2012881_POSITIVE 247 50 6 76 32 smit:SMT_1177|3end_immunoglobulin_A1_protease_1129193..1129343_NEGATIVE 239 50 1 115 66 smit:SMT_747|3end_transcriptional_regulator,_LytR_family_651343..651493_NEGATIVE 237 55 16 50 2 smit:SMT_1946|3end_sucrose-6-phosphate_hydrolase_1881205..1881355_POSITIVE 235 50 8 113 70 smit:SMT_1806|3end_aminoacylase/N-acyl-L-amino_acid_amidohydrolase/hippurate_hydrolase_1781644..1781794_POSITIVE 233 50 25 37 6 smit:SMT_967|3end_K01992_ABC-2_type_transport_system_permease_protein_912215..912365_NEGATIVE 232 55 18 44 12 smit:SMT_1379|3end_thymidylate_kinase_1356800..1356950_NEGATIVE 229 50 4 69 23 smit:SMT_2127|3end_exodeoxyribonuclease_V_alpha_chain_2009613..2009763_POSITIVE 227 46 9 149 107 smit:SMT_347|3end_hypothetical_protein_285881..286031_NEGATIVE 226 49 9 46 2 smit:SMT_597|3end_K08974_putative_membrane_protein_513075..513225_NEGATIVE