Origin IGS: gtctgttgttatttaagtgaattcaaccttaatttgattgtaggtgactgttaattgaaggaatggaactattgtaatgatgattttacttgtccagtagtggcgtattaaatgaggagtgatatgtaagcttgtctcttcgtttacgaaatcaaaatttttatgaaattgatagaatccctaactttctcttaatcttgaccgttttgttgtacagcggttaaagcttgaaaagccgagaatgtaaacctttgacaaaatttgtgaactgtaggataatggagaggaattaaggattagttctatattatggactagaaaagaccccaagctaacttccacattaagcttagggtcttttttgacactatctatcttcgtttagccatttatcgactaagcgaaggatgacaccgaccacaatcggtccgatgatagttttaaggattagttccatcatgagctatctcacctcctttcgtaggcggtgtagtagtgccgtataatattattccacataagtgttaaactcgggagtcacccaatcgggtggcttttttgtttgtggcttggttttttgatataatagagca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgctctattatatcaaaaaaccaagccacaaacaaaaaagccacccgattgggtgactcccgagtttaacacttatgtggaataatattatacggcactactacaccgcctacgaaaggaggtgagatagctcatgatggaactaatccttaaaactatcatcggaccgattgtggtcggtgtcatccttcgcttagtcgataaatggctaaacgaagatagatagtgtcaaaaaagaccctaagcttaatgtggaagttagcttggggtcttttctagtccataatatagaactaatccttaattcctctccattatcctacagttcacaaattttgtcaaaggtttacattctcggcttttcaagctttaaccgctgtacaacaaaacggtcaagattaagagaaagttagggattctatcaatttcataaaaattttgatttcgtaaacgaagagacaagcttacatatcactcctcatttaatacgccactactggacaagtaaaatcatcattacaatagttccattccttcaattaacagtcacctacaatcaaattaaggttgaattcacttaaataacaacagac Mask Tandem Repeat Region ================================================ gtctgttgttatttaagtgaattcaaccttaatttgattgtaggtgactgttaattgaaggaatggaactattgtaatgatgattttacttgtccagtagtggcgtattaaatgaggagtgatatgtaagcttgtctcttcgtttacgaaatcaaaatttttatgaaattgatagaatccctaactttctcttaatcttgaccgttttgttgtacagcggttaaagcttgaaaagccgagaatgtaaacctttgacaaaatttgtgaactgtaggataatggagaggaattaaggattagttctatattatggactagaaaagaccccaagctaacttccacattaagcttagggtcttttttgacactatctatcttcgtttagccatttatcgactaagcgaaggatgacaccgaccacaatcggtccgatgatagttttaaggattagttccatcatgagctatctcacctcctttcgtaggcggtgtagtagtgccgtataatattattccacataagtgttaaactcgggagtcacccaatcgggtggcttttttgtttgtggcttggttttttgatataatagagca Find is-nt database================================================ Query_seq: SMT_211:SMT_212|SMT_211:SMT_212:hypothetical protein predicted by Glimmer/Critica:Holliday junction DNA helicase RuvB:->->:144377..144969 593 gtctgttgttatttaagtgaattcaaccttaatttgattgtaggtgactgttaattgaaggaatggaactattgtaatgatgattttacttgtccagtagtggcgtattaaatgaggagtgatatgtaagcttgtctcttcgtttacgaaatcaaaatttttatgaaattgatagaatccctaactttctcttaatcttgaccgttttgttgtacagcggttaaagcttgaaaagccgagaatgtaaacctttgacaaaatttgtgaactgtaggataatggagaggaattaaggattagttctatattatggactagaaaagaccccaagctaacttccacattaagcttagggtcttttttgacactatctatcttcgtttagccatttatcgactaagcgaaggatgacaccgaccacaatcggtccgatgatagttttaaggattagttccatcatgagctatctcacctcctttcgtaggcggtgtagtagtgccgtataatattattccacataagtgttaaactcgggagtcacccaatcgggtggcttttttgtttgtggcttggttttttgatataatagagca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_211:SMT_212|SMT_211:SMT_212:hypothetical protein predicted by Glimmer/Critica:Holliday junction DNA helicase RuvB:->->:144377..144969 593 gtctgttgttatttaagtgaattcaaccttaatttgattgtaggtgactgttaattgaaggaatggaactattgtaatgatgattttacttgtccagtagtggcgtattaaatgaggagtgatatgtaagcttgtctcttcgtttacgaaatcaaaatttttatgaaattgatagaatccctaactttctcttaatcttgaccgttttgttgtacagcggttaaagcttgaaaagccgagaatgtaaacctttgacaaaatttgtgaactgtaggataatggagaggaattaaggattagttctatattatggactagaaaagaccccaagctaacttccacattaagcttagggtcttttttgacactatctatcttcgtttagccatttatcgactaagcgaaggatgacaccgaccacaatcggtccgatgatagttttaaggattagttccatcatgagctatctcacctcctttcgtaggcggtgtagtagtgccgtataatattattccacataagtgttaaactcgggagtcacccaatcgggtggcttttttgtttgtggcttggttttttgatataatagagca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_211:SMT_212|SMT_211:SMT_212:hypothetical protein predicted by Glimmer/Critica:Holliday junction DNA helicase RuvB:->->:144377..144969 593 gtctgttgttatttaagtgaattcaaccttaatttgattgtaggtgactgttaattgaaggaatggaactattgtaatgatgattttacttgtccagtagtggcgtattaaatgaggagtgatatgtaagcttgtctcttcgtttacgaaatcaaaatttttatgaaattgatagaatccctaactttctcttaatcttgaccgttttgttgtacagcggttaaagcttgaaaagccgagaatgtaaacctttgacaaaatttgtgaactgtaggataatggagaggaattaaggattagttctatattatggactagaaaagaccccaagctaacttccacattaagcttagggtcttttttgacactatctatcttcgtttagccatttatcgactaagcgaaggatgacaccgaccacaatcggtccgatgatagttttaaggattagttccatcatgagctatctcacctcctttcgtaggcggtgtagtagtgccgtataatattattccacataagtgttaaactcgggagtcacccaatcgggtggcttttttgtttgtggcttggttttttgatataatagagca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 1 Max: 171 Len: 171 Subject: UniRef90_Q3BLW3 Cluster: Integrase; n=1; Phage SK137|Rep: Integrase - Phage SK137 HSP 1 e-value: 5.0E-7 bit: 56.6 Len: 171 Query Start:1 Query End:171 Subject Strand: null Subject Start: 308 Subject End: 375 Q F H K N F D F V N E E T S L H I T P H L I R H Y W T S K I I I T I V P F L Q L - - - - - - - - - - - T V T Y N Q I K V E F T * I T T D ...................................................................................................................................................................................................................................................................................................................................................................................................................................... Q L N R N F N L V N E A T G L H V T P H M M R H F F T T Q S I I A G V P L E Q L S Q A L G H T K V Y M T D R Y N Q V E D E L A E A T T D ...................................................................................................................................................................................................................................................................................................................................................................................................................................... nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatagaatccctaactttctcttaatcttgaccgttttgttgtacagcggttaaagcttgaaaagccgagaatgtaaacctttgacaaaatttgtgaactgtaggataatggagaggaattaaggattagttctatattatggactagaaaagaccccaagctaacttccacattaagcttagggtcttttttgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngagctatctcacctcctttcgtaggcggtgtagtagtgccgtataatattattccacataagtgttaaactcgggagtcacccaatcgggtggcttttttgtttgtggcttggttttttgatataatagagca Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 132 End: 221 ................................................................................................................................... M S L R L R N Q N F Y E I D R I P N F L L I L T V L L Y S G .................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 32 Strand: + Start: 408 End: 503 ....................................................................................................................................................................................................................................................................................................................................................................................................................... M T P T T I G P M I V L R I S S I M S Y L T S F R R R C S S A V .......................................................................................... Protein_Len: 30 Strand: - Start: 371 End: 460 .................................................................................................................................................................................................................................................................................................................................................................................. R D E N L W K D V L R L I V G V V I P G I I T K L I L E M M ..................................................................................................................................... Protein_Len: 32 Strand: - Start: 195 End: 290 .................................................................................................................................................................................................. D Q G N Q Q V A T L A Q F A S F T F R Q C F K H V T P Y H L P M ............................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 184 End: 375 ....................................................................................................................................................................................... R S R K T T C R N F S S F G L I Y V K S L I Q S S Y S L P S S N L I L E I N H V L F S G L S V E V N L K P D K K V S D M .......................................................................................................................................................................................................................... Protein_Len: 36 Strand: - Start: 16 End: 123 ............... T F E V K I Q N Y T V T L Q L F P V I T I I I K S T W Y H R I L H P T M ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatagaatccctaactttctcttaatcttgaccgttttgttgtacagcggttaaagcttgaaaagccgagaatgtaaacctttgacaaaatttgtgaactgtaggataatggagaggaattaaggattagttctatattatggactagaaaagaccccaagctaacttccacattaagcttagggtcttttttgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngagctatctcacctcctttcgtaggcggtgtagtagtgccgtataatattattccacataagtgttaaactcgggagtcacccaatcgggtggcttttttgtttgtggcttggttttttgatataatagagca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 86 Start: 251 End: 280 ..........................................................................................................................................................................................................................................................TTTGACAAAATTTGTGAACTGTAGGATAAT......................................................................................................................................................................................................................................................................................................................... PromScan Matrix: RpoD-17 Strand: + Score: 83 Start: 558 End: 587 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTGTTTGTGGCTTGGTTTTTTGATATAAT...... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatagaatccctaactttctcttaatcttgaccgttttgttgtacagcggttaaagcttgaaaagccgagaatgtaaacctttgacaaaatttgtgaactgtaggataatggagaggaattaaggattagttctatattatggactagaaaagaccccaagctaacttccacattaagcttagggtcttttttgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngagctatctcacctcctttcgtaggcggtgtagtagtgccgtataatattattccacataagtgttaaactcgggagtcacccaatcgggtggcttttttgtttgtggcttggttttttgatataatagagca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 78 HP_score: -5.7 Tail_Score: -5.04385 Start: 323 End: 357 Full_Region: TTATGGACTAGAAAA GACCCCAAGCTAACT TCCAC ATTAAGCTTAGGGTC TTTTTTGACACTATC ..................................................................................................................................................................................................................................................................................................................................GACCCCAAGCTAACTTCCACATTAAGCTTAGGGTC............................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 82 HP_score: -7.9 Tail_Score: -4.4767 Start: 323 End: 357 Full_Region: gatagtgtcaaaaaa gaccctaagcttaat gtgga agttagcttggggtc ttttctagtccataa ..................................................................................................................................................................................................................................................................................................................................gaccctaagcttaatgtggaagttagcttggggtc............................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 100 HP_score: -9.1 Tail_Score: -5.72622 Start: 537 End: 554 Full_Region: GTGTTAAACTCGGGA GTCACCC AATC GGGTGGC TTTTTTGTTTGTGGC ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTCACCCAATCGGGTGGC....................................... Find igs database================================================ Query_seq: SMT_211:SMT_212|SMT_211:SMT_212:hypothetical protein predicted by Glimmer/Critica:Holliday junction DNA helicase RuvB:->->:144377..144969 593 nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatagaatccctaactttctcttaatcttgaccgttttgttgtacagcggttaaagcttgaaaagccgagaatgtaaacctttgacaaaatttgtgaactgtaggataatggagaggaattaaggattagttctatattatggactagaaaagaccccaagctaacttccacattaagcttagggtcttttttgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngagctatctcacctcctttcgtaggcggtgtagtagtgccgtataatattattccacataagtgttaaactcgggagtcacccaatcgggtggcttttttgtttgtggcttggttttttgatataatagagca Intra-Species Hit: Count: 2 Min: 172 Max: 593 Len: 422 Subject: smit_SMT_211_SMT_212|hypothetical protein predicted by Glimmer/Critica:Holliday junction DNA helicase RuvB|POSITIVE:POSITIVE|[144377,144969]|593 HSP 1 e-value: 1.0E-104 bit: 381.0 Len: 192 Query Start:172 Query End:363 Subject Strand: POSITIVE Subject Start: 172 Subject End: 363 ...........................................................................................................................................................................atagaatccctaactttctcttaatcttgaccgttttgttgtacagcggttaaagcttgaaaagccgagaatgtaaacctttgacaaaatttgtgaactgtaggataatggagaggaattaaggattagttctatattatggactagaaaagaccccaagctaacttccacattaagcttagggtctttttt...................................................................................................................................................................................................................................... ...........................................................................................................................................................................atagaatccctaactttctcttaatcttgaccgttttgttgtacagcggttaaagcttgaaaagccgagaatgtaaacctttgacaaaatttgtgaactgtaggataatggagaggaattaaggattagttctatattatggactagaaaagaccccaagctaacttccacattaagcttagggtctttttt...................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-68 bit: 262.0 Len: 132 Query Start:462 Query End:593 Subject Strand: POSITIVE Subject Start: 462 Subject End: 593 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................agctatctcacctcctttcgtaggcggtgtagtagtgccgtataatattattccacataagtgttaaactcgggagtcacccaatcgggtggcttttttgtttgtggcttggttttttgatataatagagca .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................agctatctcacctcctttcgtaggcggtgtagtagtgccgtataatattattccacataagtgttaaactcgggagtcacccaatcgggtggcttttttgtttgtggcttggttttttgatataatagagca Subject: smit_SMT_215_SMT_216|leucyl-tRNA synthetase:Holliday junction DNA helicase RuvB|POSITIVE:POSITIVE|[148052,148396]|345 HSP 1 e-value: 1.0E-68 bit: 262.0 Len: 132 Query Start:462 Query End:593 Subject Strand: POSITIVE Subject Start: 214 Subject End: 345 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................agctatctcacctcctttcgtaggcggtgtagtagtgccgtataatattattccacataagtgttaaactcgggagtcacccaatcgggtggcttttttgtttgtggcttggttttttgatataatagagca .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................agctatctcacctcctttcgtaggcggtgtagtagtgccgtataatattattccacataagtgttaaactcgggagtcacccaatcgggtggcttttttgtttgtggcttggttttttgatataatagagca HSP 2 e-value: 1.0E-6 bit: 56.0 Len: 44 Query Start:320 Query End:363 Subject Strand: POSITIVE Subject Start: 74 Subject End: 115 ...............................................................................................................................................................................................................................................................................................................................aaagaccccaagctaacttccacattaagcttagggtctttttt...................................................................................................................................................................................................................................... ...............................................................................................................................................................................................................................................................................................................................aaagaccccaagctaac-tccacattaagc-tagggtctttttt...................................................................................................................................................................................................................................... Inter-species Hit: Count: 4 Min: 172 Max: 592 Len: 421 Subject: NC_008533_SPD_0240_SPD_0241|acetyltransferase, GNAT family protein:Holliday junction DNA helicase RuvB|POSITIVE:POSITIVE|[231089,232128]|1040 HSP 1 e-value: 1.0E-50 bit: 202.0 Len: 130 Query Start:463 Query End:592 Subject Strand: POSITIVE Subject Start: 910 Subject End: 1039 ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................gctatctcacctcctttcgtaggcggtgtagtagtgccgtataatattattccacataagtgttaaactcgggagtcacccaatcgggtggcttttttgtttgtggcttggttttttgatataatagagc. ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................gctatctcacctcctttcgtaggcggtgtagcagtgtcgtataatattataccacataagtgctaaacttaggagtcacccaatcaggtggcttttttgtttgtggcttggttttttgatataatagagc. HSP 2 e-value: 1.0E-16 bit: 89.7 Len: 93 Query Start:241 Query End:333 Subject Strand: POSITIVE Subject Start: 689 Subject End: 781 ................................................................................................................................................................................................................................................aatgtaaacctttgacaaaatttgtgaactgtaggataatggagaggaattaaggattagttctatattatggactagaaaagaccccaagct.................................................................................................................................................................................................................................................................... ................................................................................................................................................................................................................................................aatgtaaacctttgacaaaattcgtgaactatgggataataaaaaggaattgagtactagttctatatcataggctagaaaagaccccaagct.................................................................................................................................................................................................................................................................... HSP 3 e-value: 1.0E-10 bit: 69.9 Len: 51 Query Start:172 Query End:222 Subject Strand: POSITIVE Subject Start: 632 Subject End: 682 ...........................................................................................................................................................................atagaatccctaactttctcttaatcttgaccgttttgttgtacagcggtt................................................................................................................................................................................................................................................................................................................................................................................... ...........................................................................................................................................................................atagaatccctaacgttctcttaatcttaaccgtcttgtcgtacagcggtt................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_003098_spr0237_spr0238|hypothetical protein:Holliday junction DNA helicase RuvB|POSITIVE:POSITIVE|[231088,232127]|1040 HSP 1 e-value: 1.0E-50 bit: 202.0 Len: 130 Query Start:463 Query End:592 Subject Strand: POSITIVE Subject Start: 910 Subject End: 1039 ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................gctatctcacctcctttcgtaggcggtgtagtagtgccgtataatattattccacataagtgttaaactcgggagtcacccaatcgggtggcttttttgtttgtggcttggttttttgatataatagagc. ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................gctatctcacctcctttcgtaggcggtgtagcagtgtcgtataatattataccacataagtgctaaacttaggagtcacccaatcaggtggcttttttgtttgtggcttggttttttgatataatagagc. HSP 2 e-value: 1.0E-16 bit: 89.7 Len: 93 Query Start:241 Query End:333 Subject Strand: POSITIVE Subject Start: 689 Subject End: 781 ................................................................................................................................................................................................................................................aatgtaaacctttgacaaaatttgtgaactgtaggataatggagaggaattaaggattagttctatattatggactagaaaagaccccaagct.................................................................................................................................................................................................................................................................... ................................................................................................................................................................................................................................................aatgtaaacctttgacaaaattcgtgaactatgggataataaaaaggaattgagtactagttctatatcataggctagaaaagaccccaagct.................................................................................................................................................................................................................................................................... HSP 3 e-value: 1.0E-10 bit: 69.9 Len: 51 Query Start:172 Query End:222 Subject Strand: POSITIVE Subject Start: 632 Subject End: 682 ...........................................................................................................................................................................atagaatccctaactttctcttaatcttgaccgttttgttgtacagcggtt................................................................................................................................................................................................................................................................................................................................................................................... ...........................................................................................................................................................................atagaatccctaacgttctcttaatcttaaccgtcttgtcgtacagcggtt................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_003028_SP_0258_SP_0259|hypothetical protein:Holliday junction DNA helicase RuvB|NEGATIVE:POSITIVE|[231466,231599]|134 HSP 1 e-value: 2.0E-46 bit: 188.0 Len: 130 Query Start:463 Query End:592 Subject Strand: POSITIVE Subject Start: 3 Subject End: 133 ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................gctatctcacctcctttcgtaggcggtgtagtagtgccgtataatattattccacataagtgttaaactcgggagtcacccaatcgggtggcttttttgtttgtggcttg-gttttttgatataatagagc. ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................gctatctcacctcctttcgtaggcggtgtagaagtgccgtataatattataccacataagtgctaaacttaggagtcacccaatcaggtggcttttttgtttgtggcttgttttttttgatataatagagc. Subject: NC_003028_SP_0256_SP_0258|acetyltransferase, GNAT family:hypothetical protein|POSITIVE:NEGATIVE|[228572,231372]|2801 HSP 1 e-value: 6.0E-12 bit: 73.8 Len: 93 Query Start:241 Query End:333 Subject Strand: POSITIVE Subject Start: 2676 Subject End: 2768 ................................................................................................................................................................................................................................................aatgtaaacctttgacaaaatttgtgaactgtaggataatggagaggaattaaggattagttctatattatggactagaaaagaccccaagct.................................................................................................................................................................................................................................................................... ................................................................................................................................................................................................................................................aatgtaaagttttgacaaaattcgtgaactatgggataataaaaaggaattgagtactagttctatatcataggctagaaaagaccccaagct.................................................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-10 bit: 69.9 Len: 51 Query Start:172 Query End:222 Subject Strand: POSITIVE Subject Start: 2619 Subject End: 2669 ...........................................................................................................................................................................atagaatccctaactttctcttaatcttgaccgttttgttgtacagcggtt................................................................................................................................................................................................................................................................................................................................................................................... ...........................................................................................................................................................................atagaatccctaacgttctcttaatcttaaccgtcttgtcgtacagcggtt................................................................................................................................................................................................................................................................................................................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAGAAUCCCUAACUUUCUCUUAAUCUUGACCGUUUUGUUGUACAGCGGUUAAAGCUUGAAAAGCCGAGAAUGUAAACCUUUGACAAAAUUUGUGAACUGUAGGAUAAUGGAGAGGAAUUAAGGAUUAGUUCUAUAUUAUGGACUAGAAAAGACCCCAAGCUAACUUCCACAUUAAGCUUAGGGUCUUUUUUGACACUAUCUAUCUUCGUUUAGCCAUUUAUCGACUAAGCGAAGGAUGACACCGACCACAAUCGGUCCGAUGAUAGUUUUAAGGAUUAGUUCCAUCAUGAGCUAUCUCACCUCCUUUCGUAGGCGGUGUAGUAGUGCCGUAUAAUAUUAUUCCACAUAAGUGUUAAACUCGGGAGUCACCCAAUCGGGUGGCUUUUUUGUUUGUGGCUUGGUUUUUUGAUAUAAUAGAGC ....(((((.(((.((((((((.((((((.(.((((..((((.(((.((((....((((......))))......)))).))))))).......)))).)))))))....))))))))))).))))).(((((((..(((((..((((((((((((.(((((.............))))).)))))))))))).((((((...(((((((((((.(.......).)))))))))))...((((((.((((.(..((...((.((((((((...(((.....))).....))))))))))....))..).)).)))))))).)))))))))))..((((((....((.(((((..(((((..(((((((((((....)))))))))))..)))))..)))))))....)))))).))))))) (-129.12) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCUCUAUUAUAUCAAAAAACCAAGCCACAAACAAAAAAGCCACCCGAUUGGGUGACUCCCGAGUUUAACACUUAUGUGGAAUAAUAUUAUACGGCACUACUACACCGCCUACGAAAGGAGGUGAGAUAGCUCAUGAUGGAACUAAUCCUUAAAACUAUCAUCGGACCGAUUGUGGUCGGUGUCAUCCUUCGCUUAGUCGAUAAAUGGCUAAACGAAGAUAGAUAGUGUCAAAAAAGACCCUAAGCUUAAUGUGGAAGUUAGCUUGGGGUCUUUUCUAGUCCAUAAUAUAGAACUAAUCCUUAAUUCCUCUCCAUUAUCCUACAGUUCACAAAUUUUGUCAAAGGUUUACAUUCUCGGCUUUUCAAGCUUUAACCGCUGUACAACAAAACGGUCAAGAUUAAGAGAAAGUUAGGGAUUCUAU ..(((((..((((..........(((..((((.....((.(((((....))))).)).....))))..(((....)))..............))).......((((.(((.....))).)))).)))).......(((((.(((.........((((((...((((((((....)))))).))...(((((.(((((((.....))))))).)))))...)))))).....(((((((((((((((.(((......)))))))))))))))))).))))))))...)))))...(((((((((((..((((....(((.((((((....(((((((....)))))))........((((.....))))......))))))..((......))...)))...)))).))))))))))).... (-111.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 384 190 141 215 159 smit:SMT_1719|5end_DNA_mismatch_repair_protein_1706842..1707072_NEGATIVE 375 322 282 132 87 smit:SMT_962|5end_putative_hemin_transport_system_ATP-binding_protein_fecE_908584..908814_NEGATIVE 326 298 252 101 47 smit:SMT_1660|5end_serine_protease_1650634..1650864_NEGATIVE 323 403 370 40 7 smit:SMT_91|5end_hypothetical_protein_22578..22808_POSITIVE 320 387 361 161 135 smit:SMT_2062|5end_phosphoglycerate_mutase_1971425..1971655_NEGATIVE 320 387 361 161 135 smit:SMT_1995|5end_phosphoglycerate_mutase_1920750..1920980_NEGATIVE 320 387 361 161 135 smit:SMT_201|5end_phosphoglycerate_mutase_131329..131559_POSITIVE 318 191 155 230 199 smit:SMT_522|5end_K02003_446719..446949_POSITIVE 316 261 224 226 186 smit:SMT_125|5end_hypothetical_protein_77664..77894_NEGATIVE 312 200 151 84 34 smit:SMT_784|5end_hypothetical_protein_696298..696528_NEGATIVE 310 334 297 185 148 smit:SMT_1202|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1164097..1164327_NEGATIVE 306 270 223 88 35 smit:SMT_1191|5end_phosphoribosylamine--glycine_ligase_1149737..1149967_NEGATIVE 303 318 272 71 11 smit:SMT_725|5end_hypothetical_protein_630415..630645_NEGATIVE 297 79 31 89 40 smit:SMT_1631|5end_phosphopantothenoylcysteine_synthase/decarboxylase_1618359..1618589_NEGATIVE 296 408 362 75 38 smit:SMT_104|5end_DNA_repair_protein_49623..49853_POSITIVE 295 407 361 205 162 smit:SMT_370|5end_acetyltransferase,_GNAT_family_304688..304918_POSITIVE 293 245 203 212 165 smit:SMT_233|5end_hypothetical_protein_172656..172886_POSITIVE 292 319 273 50 4 smit:SMT_1813|5end_amino_acid_transporter_1787142..1787372_POSITIVE 291 288 243 171 123 smit:SMT_1541|5end_isopentenyl-diphosphate_delta-isomerase_1526093..1526323_NEGATIVE 290 255 211 78 27 smit:SMT_2037|5end_lysine_decarboxylase_1948260..1948490_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 375 322 282 130 85 smit:SMT_963|3end_K02015_iron_complex_transport_system_permease_protein_908586..908736_NEGATIVE 312 200 151 75 25 smit:SMT_785|3end_hypothetical_protein_696307..696457_NEGATIVE 310 334 297 61 24 smit:SMT_1203|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1164221..1164371_NEGATIVE 307 320 273 148 103 smit:SMT_302|3end_K07444_putative_N6-adenine-specific_DNA_methylase_245319..245469_POSITIVE 305 265 226 58 6 smit:SMT_1695|3end_ribulose-phosphate_3-epimerase_1678299..1678449_POSITIVE 303 318 272 74 14 smit:SMT_726|3end_ImpB/MucB/SamB_family_protein_630412..630562_NEGATIVE 297 79 31 77 28 smit:SMT_1632|3end_hypothetical_protein_1618371..1618521_NEGATIVE 292 330 281 83 29 smit:SMT_1348|3end_exoribonuclease_II_1325148..1325298_NEGATIVE 288 260 212 84 44 smit:SMT_1190|3end_phosphoribosylaminoimidazole_carboxylase,_catalytic_subunit_1147741..1147891_NEGATIVE 283 237 193 129 81 smit:SMT_1006|3end_6-pyruvoyl_tetrahydrobiopterin_synthase,_putative_947122..947272_POSITIVE 282 300 253 145 109 smit:SMT_378|3end_hypothetical_protein_predicted_by_Glimmer/Critica_316083..316233_POSITIVE 281 60 17 139 87 smit:SMT_2168|3end_threonyl-tRNA_synthetase_2041262..2041412_POSITIVE 281 60 17 139 87 smit:SMT_2150|3end_threonyl-tRNA_synthetase_2024905..2025055_POSITIVE 281 60 17 139 87 smit:SMT_2146|3end_threonyl-tRNA_synthetase_2022280..2022430_POSITIVE 281 60 17 139 87 smit:SMT_2143|3end_threonyl-tRNA_synthetase_2020526..2020676_POSITIVE 281 60 17 139 87 smit:SMT_970|3end_threonyl-tRNA_synthetase_917795..917945_POSITIVE 278 195 153 135 82 smit:SMT_1244|3end_putative_repressor_protein_1205149..1205299_POSITIVE 275 419 371 82 34 smit:SMT_2033|3end_hydrolase,_haloacid_dehalogenase-like_family_1946739..1946889_POSITIVE 274 192 151 147 95 smit:SMT_1813|3end_amino_acid_transporter_1788232..1788382_POSITIVE 269 325 288 72 34 smit:SMT_773|3end_UDP-N-acetylenolpyruvoylglucosamine_reductase_683460..683610_NEGATIVE