Origin IGS: atttccaaagctatcggtaatgatnnnnnttaattaattaannnnntttaacttacatgcagcagcgtgaagtkggttcccgtggtgaaatgtcatggatgattatctaaaacgacccaattcccgaatttattcagctggag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctccagctgaataaattcgggaattgggtcgttttagataatcatccatgacatttcaccacgggaaccmacttcacgctgctgcatgtaagttaaannnnnttaattaattaannnnnatcattaccgatagctttggaaat Mask Tandem Repeat Region ================================================ atttccaaagctatcggtaatgatnnnnnttaattaattaannnnntttaacttacatgcagcagcgtgaagtkggttcccgtggtgaaatgtcatggatgattatctaaaacgacccaattcccgaatttattcagctggag Find is-nt database================================================ Query_seq: SMT_2118:SMT_2119|SMT_2118:SMT_2119:putative two-component sensor histidine kinase:mercuric reductase:<-->:2005580..2005722 143 atttccaaagctatcggtaatgatnnnnnttaattaattaannnnntttaacttacatgcagcagcgtgaagtkggttcccgtggtgaaatgtcatggatgattatctaaaacgacccaattcccgaatttattcagctggag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_2118:SMT_2119|SMT_2118:SMT_2119:putative two-component sensor histidine kinase:mercuric reductase:<-->:2005580..2005722 143 atttccaaagctatcggtaatgatnnnnnttaattaattaannnnntttaacttacatgcagcagcgtgaagtkggttcccgtggtgaaatgtcatggatgattatctaaaacgacccaattcccgaatttattcagctggag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_2118:SMT_2119|SMT_2118:SMT_2119:putative two-component sensor histidine kinase:mercuric reductase:<-->:2005580..2005722 143 atttccaaagctatcggtaatgatnnnnnttaattaattaannnnntttaacttacatgcagcagcgtgaagtkggttcccgtggtgaaatgtcatggatgattatctaaaacgacccaattcccgaatttattcagctggag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atttccaaagctatcggtaatgatnnnnnttaattaattaannnnntttaacttacatgcagcagcgtgaagtkggttcccgtggtgaaatgtcatggatgattatctaaaacgacccaattcccgaatttattcagctggag Predict ORF larger than 30AA ================================================ atttccaaagctatcggtaatgatnnnnnttaattaattaannnnntttaacttacatgcagcagcgtgaagtkggttcccgtggtgaaatgtcatggatgattatctaaaacgacccaattcccgaatttattcagctggag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atttccaaagctatcggtaatgatnnnnnttaattaattaannnnntttaacttacatgcagcagcgtgaagtkggttcccgtggtgaaatgtcatggatgattatctaaaacgacccaattcccgaatttattcagctggag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_2118:SMT_2119|SMT_2118:SMT_2119:putative two-component sensor histidine kinase:mercuric reductase:<-->:2005580..2005722 143 atttccaaagctatcggtaatgatnnnnnttaattaattaannnnntttaacttacatgcagcagcgtgaagtkggttcccgtggtgaaatgtcatggatgattatctaaaacgacccaattcccgaatttattcagctggag Intra-Species Hit: Count: 1 Min: 1 Max: 143 Len: 143 Subject: smit_SMT_2118_SMT_2119|putative two-component sensor histidine kinase:mercuric reductase|NEGATIVE:POSITIVE|[2005580,2005722]|143 HSP 1 e-value: 4.0E-57 bit: 221.0 Len: 143 Query Start:1 Query End:143 Subject Strand: POSITIVE Subject Start: 1 Subject End: 143 atttccaaagctatcggtaatgatnnnnnttaattaattaannnnntttaacttacatgcagcagcgtgaagtkggttcccgtggtgaaatgtcatggatgattatctaaaacgacccaattcccgaatttattcagctggag atttccaaagctatcggtaatgatnnnnnttaattaattaannnnntttaacttacatgcagcagcgtgaagtkggttcccgtggtgaaatgtcatggatgattatctaaaacgacccaattcccgaatttattcagctggag Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUCCAAAGCUAUCGGUAAUGAUNNNNNUUAAUUAAUUAANNNNNUUUAACUUACAUGCAGCAGCGUGAAGUKGGUUCCCGUGGUGAAAUGUCAUGGAUGAUUAUCUAAAACGACCCAAUUCCCGAAUUUAUUCAGCUGGAG ...((((..(((.((((.(((........(((..((((((.......(..((((.(((((....)))))))))..)...((((((.......)))))).))))))..))).........))).))))........))))))). (-25.93) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUCCAGCUGAAUAAAUUCGGGAAUUGGGUCGUUUUAGAUAAUCAUCCAUGACAUUUCACCACGGGAACCMACUUCACGCUGCUGCAUGUAAGUUAAANNNNNUUAAUUAAUUAANNNNNAUCAUUACCGAUAGCUUUGGAAAU .((((((((.........(((((....(((((....(((....))).)))))..))).)).(((.............((....)).....((((((......))))))..................))).))))..))))... (-20.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 919 100 51 191 142 smit:SMT_2118|5end_putative_two-component_sensor_histidine_kinase_2005489..2005719_NEGATIVE 298 105 62 75 36 smit:SMT_650|5end_pullulanase_559832..560062_NEGATIVE 296 99 58 54 12 smit:SMT_1111|5end_hypothetical_protein_1051409..1051639_NEGATIVE 276 94 53 150 94 smit:SMT_532|5end_30S_ribosomal_protein_S16_453076..453306_POSITIVE 261 120 71 105 47 smit:SMT_452|5end_phosphomethylpyrimidine_kinase_390568..390798_NEGATIVE 251 108 63 59 8 smit:SMT_587|5end_ATP_synthase_epsilon_chain_503950..504180_POSITIVE 250 120 75 209 166 smit:SMT_1828|5end_PTS_system,_galactose-specific_IIC_component_1797668..1797898_POSITIVE 250 120 75 109 66 smit:SMT_1827|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1797568..1797798_POSITIVE 249 119 76 147 97 smit:SMT_2062|5end_phosphoglycerate_mutase_1971425..1971655_NEGATIVE 249 119 76 147 97 smit:SMT_1995|5end_phosphoglycerate_mutase_1920750..1920980_NEGATIVE 249 140 96 203 163 smit:SMT_665|5end_NAD+_kinase_576745..576975_NEGATIVE 249 119 76 147 97 smit:SMT_201|5end_phosphoglycerate_mutase_131329..131559_POSITIVE 248 100 52 204 146 smit:SMT_959|5end_1-acyl-sn-glycerol-3-phosphate_acyltransferase_905283..905513_POSITIVE 247 139 91 135 86 smit:SMT_1188|5end_hypothetical_protein_1146653..1146883_NEGATIVE 245 94 53 150 94 smit:SMT_529|5end_30S_ribosomal_protein_S16_452177..452407_POSITIVE 244 84 52 156 120 smit:SMT_1416|5end_hypothetical_protein_1400829..1401059_NEGATIVE 242 97 56 47 2 smit:SMT_1569|5end_dihydroxy-acid_dehydratase_1561285..1561515_POSITIVE 242 104 64 181 139 smit:SMT_1327|5end_K03402_transcriptional_regulator_of_arginine_metabolism_1303746..1303976_POSITIVE 240 99 60 87 49 smit:SMT_155|5end_hypothetical_protein_102704..102934_NEGATIVE 235 140 93 161 119 smit:SMT_840|5end_tRNA_nucleotidyltransferase_(CCA-adding_enzyme)_787261..787491_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 321 99 59 72 26 smit:SMT_1953|3end_hypothetical_protein_1888107..1888257_POSITIVE 296 99 58 76 34 smit:SMT_1112|3end_K02248_competence_protein_ComGF_1051387..1051537_NEGATIVE 276 94 53 69 13 smit:SMT_531|3end_hypothetical_protein_predicted_by_Glimmer/Critica_453075..453225_POSITIVE 250 120 75 83 40 smit:SMT_1826|3end_ATP_synthase_beta_chain_1797622..1797772_POSITIVE 248 100 52 91 33 smit:SMT_2024|3end_hypothetical_protein_1940690..1940840_POSITIVE 248 100 52 80 22 smit:SMT_2002|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1924145..1924295_NEGATIVE 248 100 52 91 33 smit:SMT_1981|3end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1912868..1913018_POSITIVE 248 100 52 96 38 smit:SMT_1971|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1903526..1903676_POSITIVE 248 100 52 80 22 smit:SMT_1966|3end_DNA_polymerase_III_alpha_subunit_1898328..1898478_POSITIVE 248 100 52 91 33 smit:SMT_1942|3end_zinc_metalloprotease_ZmpB,_truncated_1878384..1878534_POSITIVE 248 100 52 80 22 smit:SMT_1927|3end_cell_division_protein_FtsH_1868408..1868558_NEGATIVE 248 100 52 91 33 smit:SMT_1926|3end_mevalonate_kinase_1868062..1868212_POSITIVE 248 100 52 91 33 smit:SMT_1887|3end_LicC_protein_1841740..1841890_POSITIVE 248 100 52 80 22 smit:SMT_1885|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1839830..1839980_NEGATIVE 248 100 52 80 22 smit:SMT_1873|3end_K02006_cobalt_transport_system_ATP-binding_protein_1832351..1832501_POSITIVE 248 100 52 96 38 smit:SMT_1852|3end_1,4-alpha-glucan_branching_enzyme_1816467..1816617_NEGATIVE 248 100 52 80 22 smit:SMT_958|3end_hypothetical_protein_predicted_by_Glimmer/Critica_905239..905389_POSITIVE 248 100 52 96 38 smit:SMT_326|3end_K02029_polar_amino_acid_transport_system_permease_protein_263217..263367_NEGATIVE 247 139 91 125 76 smit:SMT_1189|3end_phosphoribosylaminoimidazole_carboxylase_ATPase_subunit_1146663..1146813_NEGATIVE 238 115 71 58 17 smit:SMT_586|3end_ATP_synthase_F1,_beta_subunit_504019..504169_POSITIVE