Origin IGS: agaaaatcccaagctaaggaagacgcaaatccaaagcagatgtagcaaagcaagccatcgacacgcgacacaaacgatgcagtaactcagctaaaacgctggggcatatcagtagattcgtgactccacgcccgtagccaaccagcagcgaagcaagcatcgatgacgcattaaagctnnnnnttaattaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttaattaannnnnagctttaatgcgtcatcgatgcttgcttcgctgctggttggctacgggcgtggagtcacgaatctactgatatgccccagcgttttagctgagttactgcatcgtttgtgtcgcgtgtcgatggcttgctttgctacatctgctttggatttgcgtcttccttagcttgggattttct Mask Tandem Repeat Region ================================================ agaaaatcccaagctaaggaagacgcaaatccaaagcagatgtagcaaagcaagccatcgacacgcgacacaaacgatgcagtaactcagctaaaacgctggggcatatcagtagattcgtgactccacgcccgtagccaaccagcagcgaagcaagcatcgatgacgcattaaagctnnnnnttaattaa Find is-nt database================================================ Query_seq: SMT_2056:SMT_2057|SMT_2056:SMT_2057:hypothetical protein predicted by Glimmer/Critica:cell division protein FtsH:-><-:1966796..1966986 191 agaaaatcccaagctaaggaagacgcaaatccaaagcagatgtagcaaagcaagccatcgacacgcgacacaaacgatgcagtaactcagctaaaacgctggggcatatcagtagattcgtgactccacgcccgtagccaaccagcagcgaagcaagcatcgatgacgcattaaagctnnnnnttaattaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_2056:SMT_2057|SMT_2056:SMT_2057:hypothetical protein predicted by Glimmer/Critica:cell division protein FtsH:-><-:1966796..1966986 191 agaaaatcccaagctaaggaagacgcaaatccaaagcagatgtagcaaagcaagccatcgacacgcgacacaaacgatgcagtaactcagctaaaacgctggggcatatcagtagattcgtgactccacgcccgtagccaaccagcagcgaagcaagcatcgatgacgcattaaagctnnnnnttaattaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_2056:SMT_2057|SMT_2056:SMT_2057:hypothetical protein predicted by Glimmer/Critica:cell division protein FtsH:-><-:1966796..1966986 191 agaaaatcccaagctaaggaagacgcaaatccaaagcagatgtagcaaagcaagccatcgacacgcgacacaaacgatgcagtaactcagctaaaacgctggggcatatcagtagattcgtgactccacgcccgtagccaaccagcagcgaagcaagcatcgatgacgcattaaagctnnnnnttaattaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agaaaatcccaagctaaggaagacgcaaatccaaagcagatgtagcaaagcaagccatcgacacgcgacacaaacgatgcagtaactcagctaaaacgctggggcatatcagtagattcgtgactccacgcccgtagccaaccagcagcgaagcaagcatcgatgacgcattaaagctnnnnnttaattaa Predict ORF larger than 30AA ================================================ Protein_Len: 48 Strand: - Start: 17 End: 160 ................ P L R L D L A S T A F C A M S V R C L R H L L E A L V S P C I L L N T V G R G Y G V L L S A L M ............................... Protein_Len: 30 Strand: - Start: 1 End: 90 S F G L S L F V C I W L L H L L A L W R C A V C V I C Y S M ..................................................................................................... agaaaatcccaagctaaggaagacgcaaatccaaagcagatgtagcaaagcaagccatcgacacgcgacacaaacgatgcagtaactcagctaaaacgctggggcatatcagtagattcgtgactccacgcccgtagccaaccagcagcgaagcaagcatcgatgacgcattaaagctnnnnnttaattaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agaaaatcccaagctaaggaagacgcaaatccaaagcagatgtagcaaagcaagccatcgacacgcgacacaaacgatgcagtaactcagctaaaacgctggggcatatcagtagattcgtgactccacgcccgtagccaaccagcagcgaagcaagcatcgatgacgcattaaagctnnnnnttaattaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_2056:SMT_2057|SMT_2056:SMT_2057:hypothetical protein predicted by Glimmer/Critica:cell division protein FtsH:-><-:1966796..1966986 191 agaaaatcccaagctaaggaagacgcaaatccaaagcagatgtagcaaagcaagccatcgacacgcgacacaaacgatgcagtaactcagctaaaacgctggggcatatcagtagattcgtgactccacgcccgtagccaaccagcagcgaagcaagcatcgatgacgcattaaagctnnnnnttaattaa Intra-Species Hit: Count: 1 Min: 1 Max: 178 Len: 178 Subject: smit_SMT_2056_SMT_2057|hypothetical protein predicted by Glimmer/Critica:cell division protein FtsH|POSITIVE:NEGATIVE|[1966796,1966986]|191 HSP 1 e-value: 1.0E-96 bit: 353.0 Len: 178 Query Start:1 Query End:178 Subject Strand: POSITIVE Subject Start: 1 Subject End: 178 agaaaatcccaagctaaggaagacgcaaatccaaagcagatgtagcaaagcaagccatcgacacgcgacacaaacgatgcagtaactcagctaaaacgctggggcatatcagtagattcgtgactccacgcccgtagccaaccagcagcgaagcaagcatcgatgacgcattaaagct............. agaaaatcccaagctaaggaagacgcaaatccaaagcagatgtagcaaagcaagccatcgacacgcgacacaaacgatgcagtaactcagctaaaacgctggggcatatcagtagattcgtgactccacgcccgtagccaaccagcagcgaagcaagcatcgatgacgcattaaagct............. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAAAAUCCCAAGCUAAGGAAGACGCAAAUCCAAAGCAGAUGUAGCAAAGCAAGCCAUCGACACGCGACACAAACGAUGCAGUAACUCAGCUAAAACGCUGGGGCAUAUCAGUAGAUUCGUGACUCCACGCCCGUAGCCAACCAGCAGCGAAGCAAGCAUCGAUGACGCAUUAAAGCU ............(((..(((.........)))..))).((((..((.......)))))).....(((.((....((((((.....((((((......))))))((.............((((....))))..(((.((......)).)))..))..)))))).)).)))......... (-39.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGCUUUAAUGCGUCAUCGAUGCUUGCUUCGCUGCUGGUUGGCUACGGGCGUGGAGUCACGAAUCUACUGAUAUGCCCCAGCGUUUUAGCUGAGUUACUGCAUCGUUUGUGUCGCGUGUCGAUGGCUUGCUUUGCUACAUCUGCUUUGGAUUUGCGUCUUCCUUAGCUUGGGAUUUUCU ((((...(((((.(((((((((..((((.((((((((..((((((((.((((....))))......))).)).)))))))).....))).))))....))))))...))).)))))......))))..........((...(((..(((.........)))..))).))......... (-51.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 395 150 101 216 155 smit:SMT_2051|5end_enoyl-CoA_hydratase_1960639..1960869_NEGATIVE 377 167 133 70 35 smit:SMT_485|5end_hypothetical_protein_421401..421631_NEGATIVE 359 169 132 58 18 smit:SMT_1382|5end_glutamate_5-kinase_1360744..1360974_NEGATIVE 351 140 97 72 29 smit:SMT_297|5end_ABC_transporter_substrate-binding_protein_-_oligopeptide_transport_238547..238777_POSITIVE 349 170 121 136 70 smit:SMT_2045|5end_K+_transport_system,_NAD-binding_component_1956139..1956369_POSITIVE 343 170 121 90 44 smit:SMT_521|5end_K02005_HlyD_family_secretion_protein_445566..445796_POSITIVE 341 167 126 177 125 smit:SMT_1931|5end_23S_rRNA_methyltransferase_1870978..1871208_NEGATIVE 337 148 101 121 85 smit:SMT_2162|5end_hypothetical_protein_2036931..2037161_POSITIVE 335 170 128 149 96 smit:SMT_489|5end_K01999_branched-chain_amino_acid_transport_system_substrate-binding_protein_423669..423899_POSITIVE 330 163 122 102 67 smit:SMT_1797|5end_threonine_dehydratase_1772684..1772914_NEGATIVE 327 170 125 71 17 smit:SMT_147|5end_K02027_multiple_sugar_transport_system_substrate-binding_protein_97406..97636_POSITIVE 327 170 125 71 17 smit:SMT_119|5end_K02027_multiple_sugar_transport_system_substrate-binding_protein_70457..70687_POSITIVE 322 158 111 62 8 smit:SMT_1293|5end_K02051_sulfonate/nitrate/taurine_transport_system_substrate-binding_protein_1254976..1255206_POSITIVE 317 151 116 223 190 smit:SMT_1078|5end_folylpolyglutamate_synthase_1016713..1016943_NEGATIVE 316 148 103 207 153 smit:SMT_862|5end_hypothetical_protein_predicted_by_Glimmer/Critica_808587..808817_POSITIVE 314 146 103 86 45 smit:SMT_446|5end_ABC-type_cobalt_transport_system,_ATPase_component_384870..385100_POSITIVE 314 147 101 222 171 smit:SMT_125|5end_hypothetical_protein_77664..77894_NEGATIVE 312 105 61 202 150 smit:SMT_303|5end_hypothetical_protein_245364..245594_POSITIVE 311 169 122 203 152 smit:SMT_1043|5end_chaperonin_GroEL_985766..985996_NEGATIVE 308 167 125 213 159 smit:SMT_794|5end_hypothetical_protein_703487..703717_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 340 154 111 147 93 smit:SMT_807|3end_hypothetical_protein_predicted_by_Glimmer/Critica_716708..716858_NEGATIVE 334 169 121 58 3 smit:SMT_1941|3end_hypothetical_protein_1877663..1877813_POSITIVE 314 146 103 85 44 smit:SMT_445|3end_hypothetical_protein_384949..385099_POSITIVE 312 105 61 77 25 smit:SMT_302|3end_K07444_putative_N6-adenine-specific_DNA_methylase_245319..245469_POSITIVE 304 124 84 111 64 smit:SMT_1042|3end_hypothetical_cytosolic_protein_984205..984355_NEGATIVE 302 94 54 48 5 smit:SMT_2092|3end_malolactic_enzyme_1987040..1987190_NEGATIVE 302 158 117 83 41 smit:SMT_1787|3end_phosphoribosylformylglycinamidine_synthase_1763668..1763818_NEGATIVE 300 157 118 150 110 smit:SMT_986|3end_threonyl-tRNA_synthetase_931684..931834_POSITIVE 299 169 124 76 3 smit:SMT_480|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_416765..416915_NEGATIVE 295 130 89 142 106 smit:SMT_1131|3end_threonine_synthase_1065271..1065421_NEGATIVE 294 138 91 144 95 smit:SMT_1202|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1163945..1164095_NEGATIVE 287 158 120 91 50 smit:SMT_1667|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1657439..1657589_NEGATIVE 284 165 128 81 34 smit:SMT_1827|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1797722..1797872_POSITIVE 284 47 2 79 24 smit:SMT_772|3end_spermidine/putrescine_ABC_transporter,_ATP-binding_protein_682143..682293_NEGATIVE 283 169 121 45 3 smit:SMT_2011|3end_lysyl-tRNA_synthetase_1929759..1929909_NEGATIVE 282 148 109 127 85 smit:SMT_485|3end_hypothetical_protein_420229..420379_NEGATIVE 281 169 121 51 8 smit:SMT_904|3end_hypothetical_protein_852294..852444_NEGATIVE 281 170 130 133 82 smit:SMT_260|3end_phage_transcriptional_repressor_200236..200386_POSITIVE 277 162 129 151 115 smit:SMT_1530|3end_K07456_DNA_mismatch_repair_protein_MutS2_1513010..1513160_NEGATIVE 274 140 98 48 2 smit:SMT_1010|3end_hypothetical_protein_948761..948911_POSITIVE