Origin IGS: aatagcatttatgcttgagatctttttctcatgaataagaataagtgggtcatccagctcacgaactagtctcgagggggggcccggtacccaattcgccctataggagtcgtannnnnttaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttaannnnntacgactcctatagggcgaattgggtaccgggccccccctcgagactagttcgtgagctggatgacccacttattcttattcatgagaaaaagatctcaagcataaatgctatt Mask Tandem Repeat Region ================================================ aatagcatttatgcttgagatctttttctcatgaataagaataagtgggtcatccagctcacgaactagtctcgagggggggcccggtacccaattcgccctataggagtcgtannnnnttaa Find is-nt database================================================ Query_seq: SMT_2042:SMT_2043|SMT_2042:SMT_2043:K04077 chaperonin GroEL (Hsp60):putative mucus binding protein:<-<-:1953980..1954102 123 aatagcatttatgcttgagatctttttctcatgaataagaataagtgggtcatccagctcacgaactagtctcgagggggggcccggtacccaattcgccctataggagtcgtannnnnttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_2042:SMT_2043|SMT_2042:SMT_2043:K04077 chaperonin GroEL (Hsp60):putative mucus binding protein:<-<-:1953980..1954102 123 aatagcatttatgcttgagatctttttctcatgaataagaataagtgggtcatccagctcacgaactagtctcgagggggggcccggtacccaattcgccctataggagtcgtannnnnttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_2042:SMT_2043|SMT_2042:SMT_2043:K04077 chaperonin GroEL (Hsp60):putative mucus binding protein:<-<-:1953980..1954102 123 aatagcatttatgcttgagatctttttctcatgaataagaataagtgggtcatccagctcacgaactagtctcgagggggggcccggtacccaattcgccctataggagtcgtannnnnttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatagcatttatgcttgagatctttttctcatgaataagaataagtgggtcatccagctcacgaactagtctcgagggggggcccggtacccaattcgccctataggagtcgtannnnnttaa Predict ORF larger than 30AA ================================================ aatagcatttatgcttgagatctttttctcatgaataagaataagtgggtcatccagctcacgaactagtctcgagggggggcccggtacccaattcgccctataggagtcgtannnnnttaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatagcatttatgcttgagatctttttctcatgaataagaataagtgggtcatccagctcacgaactagtctcgagggggggcccggtacccaattcgccctataggagtcgtannnnnttaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_2042:SMT_2043|SMT_2042:SMT_2043:K04077 chaperonin GroEL (Hsp60):putative mucus binding protein:<-<-:1953980..1954102 123 aatagcatttatgcttgagatctttttctcatgaataagaataagtgggtcatccagctcacgaactagtctcgagggggggcccggtacccaattcgccctataggagtcgtannnnnttaa Intra-Species Hit: Count: 1 Min: 1 Max: 114 Len: 114 Subject: smit_SMT_2042_SMT_2043|K04077 chaperonin GroEL (Hsp60):putative mucus binding protein|NEGATIVE:NEGATIVE|[1953980,1954102]|123 HSP 1 e-value: 4.0E-46 bit: 184.0 Len: 114 Query Start:1 Query End:114 Subject Strand: POSITIVE Subject Start: 1 Subject End: 114 aatagcatttatgcttgagatctttttctcatgaataagaataagtgggtcatccagctcacgaactagtctcgannnnnnncccggtacccaattcgccctataggagtcgta......... aatagcatttatgcttgagatctttttctcatgaataagaataagtgggtcatccagctcacgaactagtctcgagggggggcccggtacccaattcgccctataggagtcgta......... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAGCAUUUAUGCUUGAGAUCUUUUUCUCAUGAAUAAGAAUAAGUGGGUCAUCCAGCUCACGAACUAGUCUCGAGGGGGGGCCCGGUACCCAAUUCGCCCUAUAGGAGUCGUA ....((((((...(((((((((((.(((....))).))))....((((((......)))))).......)))))))..(((((..((........)))))))....)))).)). (-26.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACGACUCCUAUAGGGCGAAUUGGGUACCGGGCCCCCCCUCGAGACUAGUUCGUGAGCUGGAUGACCCACUUAUUCUUAUUCAUGAGAAAAAGAUCUCAAGCAUAAAUGCUAUU ............((((.(....((((.....))))))))).((((((..((((((((..((((((.....))))))...))))))))....)).)))).((((....))))... (-26.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1144 100 51 191 142 smit:SMT_2042|5end_K04077_chaperonin_GroEL_(Hsp60)_1953889..1954119_NEGATIVE 370 86 41 67 18 smit:SMT_350|5end_immunity_protein_BlpL_289864..290094_POSITIVE 363 100 54 196 139 smit:SMT_325|5end_K02029_polar_amino_acid_transport_system_permease_protein_263023..263253_NEGATIVE 352 104 62 196 149 smit:SMT_308|5end_trans-2-enoyl-ACP_reductase_II_249439..249669_POSITIVE 345 92 54 104 51 smit:SMT_999|5end_hypothetical_protein_predicted_by_Glimmer/Critica_941642..941872_NEGATIVE 342 100 54 191 144 smit:SMT_2002|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1924405..1924635_NEGATIVE 342 100 54 230 183 smit:SMT_1966|5end_DNA_polymerase_III_alpha_subunit_1897934..1898164_POSITIVE 342 100 54 191 144 smit:SMT_1942|5end_zinc_metalloprotease_ZmpB,_truncated_1877882..1878112_POSITIVE 342 100 54 231 184 smit:SMT_1926|5end_mevalonate_kinase_1867692..1867922_POSITIVE 342 100 54 212 165 smit:SMT_1885|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1840057..1840287_NEGATIVE 341 87 43 84 36 smit:SMT_929|5end_hypothetical_protein_883342..883572_NEGATIVE 341 87 43 208 160 smit:SMT_928|5end_hypothetical_protein_883218..883448_NEGATIVE 330 99 54 217 161 smit:SMT_1972|5end_uncharacterized_conserved_phage_related_protein_1903662..1903892_POSITIVE 330 95 55 209 167 smit:SMT_1668|5end_K+_transport_system,_NAD-binding_component_1658288..1658518_NEGATIVE 329 103 61 196 146 smit:SMT_1129|5end_hypothetical_protein_1063926..1064156_NEGATIVE 322 100 55 183 123 smit:SMT_595|5end_phosphoglycerate_mutase_512593..512823_NEGATIVE 321 109 74 200 150 smit:SMT_2051|5end_enoyl-CoA_hydratase_1960639..1960869_NEGATIVE 317 87 47 127 75 smit:SMT_2052|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1961591..1961821_NEGATIVE 316 108 68 120 68 smit:SMT_1457|5end_16S_rRNA_m(2)G_1207_methyltransferase_1440209..1440439_NEGATIVE 316 57 12 115 68 smit:SMT_215|5end_leucyl-tRNA_synthetase_147045..147275_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 399 109 63 68 12 smit:SMT_1559|3end_6-phosphofructokinase_1544614..1544764_NEGATIVE 352 104 62 78 31 smit:SMT_307|3end_K02078_acyl_carrier_protein_249401..249551_POSITIVE 316 57 12 56 9 smit:SMT_214|3end_leucyl-tRNA_synthetase_147066..147216_POSITIVE 308 107 75 71 40 smit:SMT_1278|3end_30S_ribosomal_protein_S2_1242332..1242482_POSITIVE 306 79 38 143 97 smit:SMT_1432|3end_hypothetical_protein_1416752..1416902_NEGATIVE 304 100 54 64 11 smit:SMT_677|3end_DNA_topoisomerase_I_585303..585453_NEGATIVE 300 89 47 86 33 smit:SMT_520|3end_glutathione_reductase_444881..445031_NEGATIVE 288 99 51 46 1 smit:SMT_1580|3end_MutT/NUDIX_family_protein_1573153..1573303_POSITIVE 288 97 51 49 1 smit:SMT_366|3end_hypothetical_protein_302398..302548_POSITIVE 287 107 66 68 26 smit:SMT_1941|3end_hypothetical_protein_1877663..1877813_POSITIVE 286 87 46 80 29 smit:SMT_478|3end_hypothetical_protein_predicted_by_Glimmer/Critica_415257..415407_POSITIVE 285 100 53 56 3 smit:SMT_1643|3end_2-isopropylmalate_synthase_1630772..1630922_POSITIVE 285 109 64 136 89 smit:SMT_1014|3end_galactokinase_950645..950795_NEGATIVE 284 104 66 36 1 smit:SMT_600|3end_ADP-ribose_pyrophosphatase_516932..517082_POSITIVE 280 87 55 37 1 smit:SMT_930|3end_ATP-dependent_RNA_helicase_883389..883539_NEGATIVE 279 106 63 89 49 smit:SMT_1726|3end_tRNA_pseudouridine_synthase_B_1712453..1712603_POSITIVE 278 87 47 69 29 smit:SMT_904|3end_hypothetical_protein_852294..852444_NEGATIVE 275 87 48 140 98 smit:SMT_833|3end_hypothetical_protein_predicted_by_Glimmer/Critica_775183..775333_NEGATIVE 274 99 57 45 2 smit:SMT_2006|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1926803..1926953_POSITIVE 273 49 6 130 74 smit:SMT_107|3end_ribose-phosphate_pyrophosphokinase_53450..53600_POSITIVE