Origin IGS: ttgcacttcaggattatttacattcccatagttagcagcaatatctactttcttctttctttcctggttnnnnnttaattaattaannnnncgctattacgccagaattcgagctcggtacccctgagtctaggtcgga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tccgacctagactcaggggtaccgagctcgaattctggcgtaatagcgnnnnnttaattaattaannnnnaaccaggaaagaaagaagaaagtagatattgctgctaactatgggaatgtaaataatcctgaagtgcaa Mask Tandem Repeat Region ================================================ ttgcacttcaggattatttacattcccatagttagcagcaatatctactttcttctttctttcctggttnnnnnttaattaattaannnnncgctattacgccagaattcgagctcggtacccctgagtctaggtcgga Find is-nt database================================================ Query_seq: SMT_2025:SMT_2026|SMT_2025:SMT_2026:hypothetical protein predicted by Glimmer/Critica:K03466 DNA segregation ATPase FtsK/SpoIIIE, S-DNA-T family:<-<-:1941856..1941994 139 ttgcacttcaggattatttacattcccatagttagcagcaatatctactttcttctttctttcctggttnnnnnttaattaattaannnnncgctattacgccagaattcgagctcggtacccctgagtctaggtcgga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_2025:SMT_2026|SMT_2025:SMT_2026:hypothetical protein predicted by Glimmer/Critica:K03466 DNA segregation ATPase FtsK/SpoIIIE, S-DNA-T family:<-<-:1941856..1941994 139 ttgcacttcaggattatttacattcccatagttagcagcaatatctactttcttctttctttcctggttnnnnnttaattaattaannnnncgctattacgccagaattcgagctcggtacccctgagtctaggtcgga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_2025:SMT_2026|SMT_2025:SMT_2026:hypothetical protein predicted by Glimmer/Critica:K03466 DNA segregation ATPase FtsK/SpoIIIE, S-DNA-T family:<-<-:1941856..1941994 139 ttgcacttcaggattatttacattcccatagttagcagcaatatctactttcttctttctttcctggttnnnnnttaattaattaannnnncgctattacgccagaattcgagctcggtacccctgagtctaggtcgga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttgcacttcaggattatttacattcccatagttagcagcaatatctactttcttctttctttcctggttnnnnnttaattaattaannnnncgctattacgccagaattcgagctcggtacccctgagtctaggtcgga Predict ORF larger than 30AA ================================================ ttgcacttcaggattatttacattcccatagttagcagcaatatctactttcttctttctttcctggttnnnnnttaattaattaannnnncgctattacgccagaattcgagctcggtacccctgagtctaggtcgga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttgcacttcaggattatttacattcccatagttagcagcaatatctactttcttctttctttcctggttnnnnnttaattaattaannnnncgctattacgccagaattcgagctcggtacccctgagtctaggtcgga Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_2025:SMT_2026|SMT_2025:SMT_2026:hypothetical protein predicted by Glimmer/Critica:K03466 DNA segregation ATPase FtsK/SpoIIIE, S-DNA-T family:<-<-:1941856..1941994 139 ttgcacttcaggattatttacattcccatagttagcagcaatatctactttcttctttctttcctggttnnnnnttaattaattaannnnncgctattacgccagaattcgagctcggtacccctgagtctaggtcgga Intra-Species Hit: Count: 1 Min: 1 Max: 139 Len: 139 Subject: smit_SMT_2025_SMT_2026|hypothetical protein predicted by Glimmer/Critica:K03466 DNA segregation ATPase FtsK/SpoIIIE, S-DNA-T family|NEGATIVE:NEGATIVE|[1941856,1941994]|139 HSP 1 e-value: 1.0E-55 bit: 216.0 Len: 139 Query Start:1 Query End:139 Subject Strand: POSITIVE Subject Start: 1 Subject End: 139 ttgcacttcaggattatttacattcccatagttagcagcaatatctactttcttctttctttcctggttnnnnnttaattaattaannnnncgctattacgccagaattcgagctcggtacccctgagtctaggtcgga ttgcacttcaggattatttacattcccatagttagcagcaatatctactttcttctttctttcctggttnnnnnttaattaattaannnnncgctattacgccagaattcgagctcggtacccctgagtctaggtcgga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGCACUUCAGGAUUAUUUACAUUCCCAUAGUUAGCAGCAAUAUCUACUUUCUUCUUUCUUUCCUGGUUNNNNNUUAAUUAAUUAANNNNNCGCUAUUACGCCAGAAUUCGAGCUCGGUACCCCUGAGUCUAGGUCGGA (((((((...(((..........)))...)))..))))...................(((..(((((.........................((......))..........((((((.....)))))))))))..))) (-19.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCCGACCUAGACUCAGGGGUACCGAGCUCGAAUUCUGGCGUAAUAGCGNNNNNUUAAUUAAUUAANNNNNAACCAGGAAAGAAAGAAGAAAGUAGAUAUUGCUGCUAACUAUGGGAAUGUAAAUAAUCCUGAAGUGCAA ..........((((((((.(((....((((..(((((((((....)))........................))))))............(((((......))))).....))))...))).....))))).))).... (-21.15) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 824 50 1 141 92 smit:SMT_2025|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1941765..1941995_NEGATIVE 805 130 92 219 181 smit:SMT_1465|5end_hypothetical_protein_1445409..1445639_POSITIVE 805 130 92 163 125 smit:SMT_389|5end_PTS_system,_galactitol-specific_IIA_component,_putative_321779..322009_POSITIVE 748 50 1 141 92 smit:SMT_2102|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1994533..1994763_NEGATIVE 684 130 97 186 153 smit:SMT_1872|5end_K03217_preprotein_translocase_YidC_subunit_1831764..1831994_NEGATIVE 278 69 28 112 81 smit:SMT_1795|5end_DNA_polymerase_III,_delta_subunit_1769847..1770077_NEGATIVE 269 46 3 220 173 smit:SMT_646|5end_K07284_sortase_A_551702..551932_NEGATIVE 267 126 93 217 180 smit:SMT_1957|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1890645..1890875_POSITIVE 265 69 21 170 132 smit:SMT_1861|5end_sucrose_operon_repressor_(SCR_operon_regulatory_protein)_1823713..1823943_POSITIVE 264 129 92 180 143 smit:SMT_1823|5end_GTP-binding_protein_1794982..1795212_POSITIVE 257 130 93 64 28 smit:SMT_1606|5end_glutamate_racemase_1595857..1596087_POSITIVE 251 130 92 214 175 smit:SMT_1450|5end_K02057_simple_sugar_transport_system_permease_protein_1433622..1433852_NEGATIVE 250 59 11 89 42 smit:SMT_288|5end_hypothetical_protein_231184..231414_POSITIVE 243 129 94 57 27 smit:SMT_1933|5end_K02006_cobalt_transport_system_ATP-binding_protein_1872222..1872452_NEGATIVE 242 68 25 105 42 smit:SMT_1130|5end_MATE_efflux_family_protein_1065210..1065440_NEGATIVE 241 128 92 121 81 smit:SMT_1475|5end_K02004_1455873..1456103_POSITIVE 238 123 93 183 148 smit:SMT_2042|5end_K04077_chaperonin_GroEL_(Hsp60)_1953889..1954119_NEGATIVE 234 130 92 66 23 smit:SMT_2052|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1961591..1961821_NEGATIVE 231 130 92 101 62 smit:SMT_1922|5end_exonuclease_RexA_1865998..1866228_NEGATIVE 231 130 92 101 62 smit:SMT_1484|5end_exonuclease_RexA_1464936..1465166_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 805 130 92 81 43 smit:SMT_2044|3end_transcriptional_activator_TipA,_putative_1955274..1955424_NEGATIVE 805 130 92 81 43 smit:SMT_2016|3end_tyrosine_recombinase_1933021..1933171_NEGATIVE 805 130 92 81 43 smit:SMT_2006|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1926803..1926953_POSITIVE 805 130 92 91 53 smit:SMT_2001|3end_membrane_protease_protein_family_1923889..1924039_POSITIVE 805 130 92 91 53 smit:SMT_1985|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1914407..1914557_NEGATIVE 805 130 92 81 43 smit:SMT_1957|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1890829..1890979_POSITIVE 805 130 92 39 1 smit:SMT_1955|3end_carbamoyl-phosphate_synthase_small_chain_1888992..1889142_NEGATIVE 805 130 92 81 43 smit:SMT_1886|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1841198..1841348_POSITIVE 805 130 92 81 43 smit:SMT_1658|3end_hypothetical_protein_1648937..1649087_POSITIVE 805 130 92 81 43 smit:SMT_1558|3end_hypothetical_protein_1543855..1544005_NEGATIVE 805 130 92 91 53 smit:SMT_1464|3end_K02030_polar_amino_acid_transport_system_substrate-binding_protein_1445361..1445511_POSITIVE 805 130 92 81 43 smit:SMT_1155|3end_ABC-type_oligopeptide_transport_system,_periplasmic_component_1099183..1099333_NEGATIVE 718 126 92 151 117 smit:SMT_388|3end_branched-chain_amino_acid_transport_system_II_carrier_protein_321851..322001_POSITIVE 501 130 105 37 12 smit:SMT_1631|3end_phosphopantothenoylcysteine_synthase/decarboxylase_1618204..1618354_NEGATIVE 278 69 28 78 47 smit:SMT_1796|3end_dihydroorotate_dehydrogenase_A_1769881..1770031_NEGATIVE 250 59 11 99 52 smit:SMT_287|3end_UDP-galactopyranose_mutase_231274..231424_POSITIVE 247 130 92 61 14 smit:SMT_1941|3end_hypothetical_protein_1877663..1877813_POSITIVE 246 126 92 128 83 smit:SMT_242|3end_PTS_system,_mannose-specific_IID_component_179163..179313_NEGATIVE 242 45 10 92 57 smit:SMT_1960|3end_UDP-galactopyranose_mutase_1894094..1894244_POSITIVE 241 128 92 119 79 smit:SMT_1474|3end_K02003_1455951..1456101_POSITIVE