Origin IGS: tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggggatacgcaggaaagaacattcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgaggannnnnttaattaattaannnnngagatttcgatttct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agaaatcgaaatctcnnnnnttaattaattaannnnntcctcaacgacaggagcacgatcatgcgcacccgtgcccaggacccaacgctgcccgaatgttctttcctgcgtatccccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga Mask Tandem Repeat Region ================================================ tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggggatacgcaggaaagaacattcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgaggannnnnttaattaattaannnnngagatttcgatttct Find is-nt database================================================ Query_seq: SMT_2024:SMT_2025|SMT_2024:SMT_2025:hypothetical protein:hypothetical protein predicted by Glimmer/Critica:-><-:1940751..1941084 334 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggggatacgcaggaaagaacattcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgaggannnnnttaattaattaannnnngagatttcgatttct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_2024:SMT_2025|SMT_2024:SMT_2025:hypothetical protein:hypothetical protein predicted by Glimmer/Critica:-><-:1940751..1941084 334 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggggatacgcaggaaagaacattcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgaggannnnnttaattaattaannnnngagatttcgatttct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_2024:SMT_2025|SMT_2024:SMT_2025:hypothetical protein:hypothetical protein predicted by Glimmer/Critica:-><-:1940751..1941084 334 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggggatacgcaggaaagaacattcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgaggannnnnttaattaattaannnnngagatttcgatttct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 62 Max: 229 Len: 168 Subject: UniRef90_Q9KHC4 Cluster: SocE; n=1; Myxococcus xanthus|Rep: SocE - Myxococcus xanthus HSP 1 e-value: 1.0E-6 bit: 54.3 Len: 168 Query Start:62 Query End:229 Subject Strand: null Subject Start: 266 Subject End: 322 ............................................................. C I N E S A N A R G E A V C V L G A L P L P R S L T R C A R S F G C G E R Y Q L - T Q R R * Y G Y P Q N Q G D T Q ......................................................................................................... ............................................................. C I R D P A T A R S E A V W V L V A L P L L R S R T R C V R S V G C G G A V S A H S P G R P Y G D P Q P Q G M A Q ......................................................................................................... tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgaggannnnnttaattaattaannnnngagatttcgatttct Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: + Start: 6 End: 134 ..... M T I L A Q G D P L E S T C R H A S S A L M N R P T R G E R R F A Y W A L F R F L A H ........................................................................................................................................................................................................ Protein_Len: 50 Strand: + Start: 44 End: 193 ........................................... M Q A C K L C I N E S A N A R G E A V C V L G A L P L P R S L T R C A R S F G C G E R Y Q L T Q R R ............................................................................................................................................. Protein_Len: 60 Strand: + Start: 73 End: 273 ........................................................................ M G Q R A G R G G L R I G R S S A S S L T D S L R S V V R L R R A V S A H S K A V I R L S T E S G G Y A G K N I R A A L ............................................................. Protein_Len: 46 Strand: + Start: 174 End: 311 ............................................................................................................................................................................. M S S L K G G N T V I H R I R G I R R K E H S G S V G S W A R V R M I V L L S L R X X L I N ....................... Protein_Len: 58 Strand: - Start: 134 End: 307 ..................................................................................................................................... Q S A A S P R E A A A L P I L E S L P P L V T I W L I L P I R L F S C E P L T P D Q A R T R M I T S R D N L X X X M ........................... tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgaggannnnnttaattaattaannnnngagatttcgatttct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgaggannnnnttaattaattaannnnngagatttcgatttct Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_2024:SMT_2025|SMT_2024:SMT_2025:hypothetical protein:hypothetical protein predicted by Glimmer/Critica:-><-:1940751..1941084 334 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgaggannnnnttaattaattaannnnngagatttcgatttct Intra-Species Hit: Count: 17 Min: 1 Max: 334 Len: 334 Subject: smit_SMT_2024_SMT_2025|hypothetical protein:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1940751,1941084]|334 HSP 1 e-value: 2.0E-29 bit: 131.0 Len: 96 Query Start:239 Query End:334 Subject Strand: POSITIVE Subject Start: 239 Subject End: 334 ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgaggannnnnttaattaattaannnnngagatttcgatttct ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgaggannnnnttaattaattaannnnngagatttcgatttct HSP 2 e-value: 2.0E-26 bit: 121.0 Len: 61 Query Start:1 Query End:61 Subject Strand: POSITIVE Subject Start: 1 Subject End: 61 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. Subject: smit_SMT_1978_SMT_1979|hypothetical protein:possible cell surface protein|NEGATIVE:NEGATIVE|[1909657,1910371]|715 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 61 Query Start:1 Query End:61 Subject Strand: NEGATIVE Subject Start: 550 Subject End: 610 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. HSP 2 e-value: 6.0E-23 bit: 109.0 Len: 59 Query Start:239 Query End:297 Subject Strand: NEGATIVE Subject Start: 314 Subject End: 372 ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... Subject: smit_SMT_1966_SMT_1967|DNA polymerase III alpha subunit:dicarboxylate/amino acid:cation (Na+ or H+) symporter (DAACS) family protein|POSITIVE:NEGATIVE|[1898389,1899587]|1199 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 61 Query Start:1 Query End:61 Subject Strand: POSITIVE Subject Start: 12 Subject End: 72 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. HSP 2 e-value: 6.0E-23 bit: 109.0 Len: 59 Query Start:239 Query End:297 Subject Strand: POSITIVE Subject Start: 406 Subject End: 464 ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... Subject: smit_SMT_1953_SMT_1954|hypothetical protein:6-phospho-beta- glucosidase|POSITIVE:POSITIVE|[1888168,1888687]|520 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 61 Query Start:1 Query End:61 Subject Strand: NEGATIVE Subject Start: 403 Subject End: 463 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. HSP 2 e-value: 6.0E-23 bit: 109.0 Len: 59 Query Start:239 Query End:297 Subject Strand: NEGATIVE Subject Start: 167 Subject End: 225 ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... Subject: smit_SMT_1946_SMT_1947|sucrose-6-phosphate hydrolase:lysine decarboxylase|POSITIVE:NEGATIVE|[1881266,1882402]|1137 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 61 Query Start:1 Query End:61 Subject Strand: POSITIVE Subject Start: 488 Subject End: 548 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. HSP 2 e-value: 6.0E-23 bit: 109.0 Len: 59 Query Start:239 Query End:297 Subject Strand: POSITIVE Subject Start: 726 Subject End: 784 ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... Subject: smit_SMT_1944_SMT_1945|hypothetical protein predicted by Glimmer/Critica:putative dihydrolipoamide S-acetyltransferase|NEGATIVE:POSITIVE|[1879585, 1880100]|516 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 61 Query Start:1 Query End:61 Subject Strand: POSITIVE Subject Start: 49 Subject End: 109 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. HSP 2 e-value: 6.0E-23 bit: 109.0 Len: 59 Query Start:239 Query End:297 Subject Strand: POSITIVE Subject Start: 287 Subject End: 345 ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... Subject: smit_SMT_1895_SMT_1896|hypothetical protein predicted by Glimmer/Critica:lysine decarboxylase|POSITIVE:NEGATIVE|[1847463,1848162]|700 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 61 Query Start:1 Query End:61 Subject Strand: POSITIVE Subject Start: 73 Subject End: 133 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. HSP 2 e-value: 6.0E-23 bit: 109.0 Len: 59 Query Start:239 Query End:297 Subject Strand: POSITIVE Subject Start: 311 Subject End: 369 ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... Subject: smit_SMT_1887_SMT_1888|LicC protein:K02026 multiple sugar transport system permease protein|POSITIVE:POSITIVE|[1841801,1842227]|427 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 61 Query Start:1 Query End:61 Subject Strand: POSITIVE Subject Start: 1 Subject End: 61 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. HSP 2 e-value: 6.0E-23 bit: 109.0 Len: 59 Query Start:239 Query End:297 Subject Strand: POSITIVE Subject Start: 239 Subject End: 297 ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... Subject: smit_SMT_1885_SMT_1886|hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1840148,1841123]|976 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 61 Query Start:1 Query End:61 Subject Strand: NEGATIVE Subject Start: 703 Subject End: 763 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. HSP 2 e-value: 9.0E-19 bit: 95.6 Len: 59 Query Start:239 Query End:297 Subject Strand: NEGATIVE Subject Start: 466 Subject End: 525 ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggc-acgggtgcgcatgatcgtgctcctgtcgttgagga..................................... ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctggcccacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... Subject: smit_SMT_1884_SMT_1885|hypothetical protein:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1839299,1839919]|621 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 61 Query Start:1 Query End:61 Subject Strand: NEGATIVE Subject Start: 550 Subject End: 610 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. HSP 2 e-value: 6.0E-23 bit: 109.0 Len: 59 Query Start:239 Query End:297 Subject Strand: NEGATIVE Subject Start: 314 Subject End: 372 ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... Subject: smit_SMT_1857_SMT_1858|hypothetical protein:K03497 chromosome partitioning protein, ParB family|POSITIVE:NEGATIVE|[1821206,1821776]|571 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 61 Query Start:1 Query End:61 Subject Strand: POSITIVE Subject Start: 88 Subject End: 148 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. HSP 2 e-value: 6.0E-23 bit: 109.0 Len: 59 Query Start:239 Query End:297 Subject Strand: POSITIVE Subject Start: 326 Subject End: 384 ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... Subject: smit_SMT_1849_SMT_1850|hypothetical protein:K01421 putative membrane protein|POSITIVE:POSITIVE|[1813865,1815201]|1337 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 61 Query Start:1 Query End:61 Subject Strand: NEGATIVE Subject Start: 922 Subject End: 982 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. HSP 2 e-value: 6.0E-23 bit: 109.0 Len: 59 Query Start:239 Query End:297 Subject Strand: NEGATIVE Subject Start: 686 Subject End: 744 ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... Subject: smit_SMT_1845_SMT_1846|K01191 alpha-mannosidase:acyl-ACP thioesterase, putative|POSITIVE:POSITIVE|[1810496,1811549]|1054 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 61 Query Start:1 Query End:61 Subject Strand: NEGATIVE Subject Start: 636 Subject End: 696 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. HSP 2 e-value: 1.0E-21 bit: 105.0 Len: 59 Query Start:239 Query End:297 Subject Strand: NEGATIVE Subject Start: 400 Subject End: 458 ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgakga..................................... Subject: smit_SMT_1685_SMT_1686|phenylalanyl-tRNA synthetase alpha chain:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1668936,1670314]|1379 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 61 Query Start:1 Query End:61 Subject Strand: NEGATIVE Subject Start: 1230 Subject End: 1290 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. HSP 2 e-value: 6.0E-23 bit: 109.0 Len: 59 Query Start:239 Query End:297 Subject Strand: NEGATIVE Subject Start: 994 Subject End: 1052 ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... Subject: smit_SMT_478_SMT_479|hypothetical protein predicted by Glimmer/Critica:K03308 neurotransmitter:Na+ symporter, NSS family|POSITIVE:NEGATIVE|[415318,415693]|376 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 61 Query Start:1 Query End:61 Subject Strand: NEGATIVE Subject Start: 252 Subject End: 312 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. HSP 2 e-value: 6.0E-23 bit: 109.0 Len: 59 Query Start:239 Query End:297 Subject Strand: NEGATIVE Subject Start: 16 Subject End: 74 ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... Subject: smit_SMT_303_SMT_304|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[245819,246719]|901 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 61 Query Start:1 Query End:61 Subject Strand: POSITIVE Subject Start: 132 Subject End: 192 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. HSP 2 e-value: 6.0E-23 bit: 109.0 Len: 59 Query Start:239 Query End:297 Subject Strand: NEGATIVE Subject Start: 618 Subject End: 676 ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... HSP 3 e-value: 9.0E-19 bit: 95.6 Len: 48 Query Start:14 Query End:61 Subject Strand: NEGATIVE Subject Start: 854 Subject End: 901 .............cctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. .............cctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. Subject: smit_SMT_1851_SMT_1852|ABC transporter, permease protein, putative:1,4-alpha-glucan branching enzyme|NEGATIVE:NEGATIVE|[1815812,1816556]|745 HSP 1 e-value: 2.0E-23 bit: 111.0 Len: 56 Query Start:6 Query End:61 Subject Strand: NEGATIVE Subject Start: 690 Subject End: 745 .....gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. .....gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctc................................................................................................................................................................................................................................................................................. HSP 2 e-value: 6.0E-23 bit: 109.0 Len: 59 Query Start:239 Query End:297 Subject Strand: NEGATIVE Subject Start: 454 Subject End: 512 ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctgggcacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... ..............................................................................................................................................................................................................................................ttcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgagga..................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUAGGUGACCAUCCUAGCACAGGGGGAUCCUCUAGAGUCGACCUGCAGGCAUGCAAGCUCUGCAUUAAUGAAUCGGCCAACGCGCGGGGAGAGGCGGUUUGCGUAUUGGGCGCUCUUCCGCUUCCUCGCUCACUGACUCGCUGCGCUCGGUCGUUCGGCUGCGGCGAGCGGUAUCAGCUCACUCAAAGGCGGUAAUACGGUUAUCCACAGAAUCAGGGGGAUACGCAGGAAAGAACAUUCGGGCAGCGUUGGGUCCUGGGCACGGGUGCGCAUGAUCGUGCUCCUGUCGUUGAGGANNNNNUUAAUUAAUUAANNNNNGAGAUUUCGAUUUCU ((((((.((((.((((.....)))))).)).)))))).......(((((((......)).))))).....((((((.((((((((((((((..(((((...((((.....))))....))))))))))))..((((.((((((((((.(((....))))).))))))))))))....((((......((.((......)).)).(((...(.(((.....))).)...)))..........)))).))))))).((((.((((((((.((((......)))).))))).))).)))).............................)))))).. (-119.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAAAUCGAAAUCUCNNNNNUUAAUUAAUUAANNNNNUCCUCAACGACAGGAGCACGAUCAUGCGCACCCGUGCCCAGGACCCAACGCUGCCCGAAUGUUCUUUCCUGCGUAUCCCCCUGAUUCUGUGGAUAACCGUAUUACCGCCUUUGAGUGAGCUGAUACCGCUCGCCGCAGCCGAACGACCGAGCGCAGCGAGUCAGUGAGCGAGGAAGCGGAAGAGCGCCCAAUACGCAAACCGCCUCUCCCCGCGCGUUGGCCGAUUCAUUAAUGCAGAGCUUGCAUGCCUGCAGGUCGACUCUAGAGGAUCCCCCUGUGCUAGGAUGGUCACCUAGA .....................................(((((.(((((.((.(((((............))))))).........(((((((((..(((((....(((((...............((((.(((.....)))))))......((((((.......)))))))))))..)))))..)).).)))))).))).))....))))).((((.((((.((..............))))))..))))..(((((((..........(((((.((......)))))))))))))).((((..((((..((((...))))..))))..)))). (-95.94) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1200 130 81 140 91 smit:SMT_2005|5end_pullulanase,_type_I_1926289..1926519_NEGATIVE 1200 130 81 208 159 smit:SMT_304|5end_hypothetical_protein_246580..246810_POSITIVE 1166 120 71 224 175 smit:SMT_1967|5end_dicarboxylate/amino_acid:cation_(Na+_or_H+)_symporter_(DAACS)_family_protein_1899683..1899913_NEGATIVE 1166 120 71 224 175 smit:SMT_1690|5end_K06148_ATP-binding_cassette,_subfamily_C,_bacterial_1674779..1675009_NEGATIVE 1166 120 71 224 175 smit:SMT_779|5end_ribonuclease_BN,_putative_688530..688760_NEGATIVE 1109 90 41 231 182 smit:SMT_2008|5end_K07485_transposase_1928662..1928892_NEGATIVE 1109 90 41 194 145 smit:SMT_1955|5end_carbamoyl-phosphate_synthase_small_chain_1889408..1889638_NEGATIVE 1109 90 41 229 180 smit:SMT_1944|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1879494..1879724_NEGATIVE 1109 90 41 115 66 smit:SMT_464|5end_ribonuclease_Z_402867..403097_POSITIVE 1109 90 41 194 145 smit:SMT_388|5end_branched-chain_amino_acid_transport_system_II_carrier_protein_321172..321402_POSITIVE 1101 60 11 228 179 smit:SMT_2016|5end_tyrosine_recombinase_1933425..1933655_NEGATIVE 1101 60 11 219 170 smit:SMT_2001|5end_membrane_protease_protein_family_1923387..1923617_POSITIVE 1101 60 11 164 115 smit:SMT_1961|5end_K07485_transposase_1894206..1894436_POSITIVE 1101 60 11 208 159 smit:SMT_1954|5end_6-phospho-beta-glucosidase_1888548..1888778_POSITIVE 1101 60 11 213 164 smit:SMT_1155|5end_ABC-type_oligopeptide_transport_system,_periplasmic_component_1099665..1099895_NEGATIVE 1094 50 1 230 181 smit:SMT_1686|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1670175..1670405_POSITIVE 1032 48 1 231 184 smit:SMT_2044|5end_transcriptional_activator_TipA,_putative_1955885..1956115_NEGATIVE 654 32 1 215 184 smit:SMT_2006|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1926472..1926702_POSITIVE 536 120 74 144 93 smit:SMT_2052|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1961591..1961821_NEGATIVE 474 179 133 210 166 smit:SMT_1982|5end_conserved_hypothetical_protein_1913036..1913266_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1223 170 121 52 3 smit:SMT_780|3end_peptidase_family_U32_688752..688902_NEGATIVE 1136 290 241 68 19 smit:SMT_478|3end_hypothetical_protein_predicted_by_Glimmer/Critica_415257..415407_POSITIVE 1109 90 41 65 16 smit:SMT_1956|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1889537..1889687_NEGATIVE 1109 90 41 60 11 smit:SMT_387|3end_hypothetical_protein_predicted_by_Glimmer/Critica_321118..321268_POSITIVE 832 260 211 60 11 smit:SMT_1874|3end_hypothetical_protein_1832623..1832773_NEGATIVE 508 190 165 26 1 smit:SMT_1968|3end_transcription-repair_coupling_factor_1899951..1900101_NEGATIVE 430 147 102 55 2 smit:SMT_1750|3end_anthranilate_phosphoribosyltransferase_1729095..1729245_POSITIVE 423 166 121 84 25 smit:SMT_1941|3end_hypothetical_protein_1877663..1877813_POSITIVE 415 130 83 53 5 smit:SMT_2044|3end_transcriptional_activator_TipA,_putative_1955274..1955424_NEGATIVE 415 130 83 53 5 smit:SMT_2016|3end_tyrosine_recombinase_1933021..1933171_NEGATIVE 415 130 83 63 15 smit:SMT_2001|3end_membrane_protease_protein_family_1923889..1924039_POSITIVE 415 130 83 53 5 smit:SMT_1957|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1890829..1890979_POSITIVE 415 130 83 53 5 smit:SMT_1886|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1841198..1841348_POSITIVE 415 130 83 53 5 smit:SMT_1658|3end_hypothetical_protein_1648937..1649087_POSITIVE 415 130 83 53 5 smit:SMT_1558|3end_hypothetical_protein_1543855..1544005_NEGATIVE 415 130 83 53 5 smit:SMT_1155|3end_ABC-type_oligopeptide_transport_system,_periplasmic_component_1099183..1099333_NEGATIVE 414 290 241 64 15 smit:SMT_1559|3end_6-phosphofructokinase_1544614..1544764_NEGATIVE 408 105 84 22 1 smit:SMT_463|3end_para-aminobenzoate_synthetase_component_I_/_4-amino-4-deoxychorismate_lyase_402839..402989_POSITIVE 406 170 121 53 1 smit:SMT_1946|3end_sucrose-6-phosphate_hydrolase_1881205..1881355_POSITIVE 406 69 28 111 56 smit:SMT_1786|3end_phenylalanyl-tRNA_synthetase_beta_chain_1763235..1763385_NEGATIVE