Origin IGS: tccgacctagactcaggggtaccgagctcgaattctggcgtaatagcgaagagggccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcnnnnnttaattaattaannnnngcaaagaaaacagagattgatgcgaccatggcttaacagcagaggaaaacctagcagctarggagaagctagggcaaaagcagatgcagctaagaaagcaatcgatggagctgatacagatattaaggtgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagamccagttagaatcagaagctaaaaaagctgtagatgaagcactaaaggcaaaagaagcagaaatkgatgcgaataatgacttaacagcagaagaaaaattagcagctaaggaggaagctaaagctaaagccgatgcagctaagaaagcaatcgatgaagctgatacagatattaaggttgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagaaccagttaagaaatcagaggctaaaaaagcaattgatgcagctctagagaagaaaatagaagaaatcgatgctcgagatgatttaacaactgaagagaaaactgcagcaaaagctgaagtagaaaataaagctaatgaggcaaaagacgcaatcgatgcagcagatacagacattaaagtagatgtaaatagtgtatctggtttaagtgat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atcacttaaaccagatacactatttacatctactttaatgtctgtatctgctgcatcgattgcgtcttttgcctcattagctttattttctacttcagcttttgctgcagttttctcttcagttgttaaatcatctcgagcatcgatttcttctattttcttctctagagctgcatcaattgcttttttagcctctgatttcttaactggttctggactaaatgaatttacatcacttaaaccagatatactatttacatcaaccttaatatctgtatcagcttcatcgattgctttcttagctgcatcggctttagctttagcttcctccttagctgctaatttttcttctgctgttaagtcattattcgcatcmatttctgcttcttttgcctttagtgcttcatctacagcttttttagcttctgattctaactggktctggactaaatgaatttacatcacttaaaccagatatactatttacatcaccttaatatctgtatcagctccatcgattgctttcttagctgcatctgcttttgccctagcttctccytagctgctaggttttcctctgctgttaagccatggtcgcatcaatctctgttttctttgcnnnnnttaattaattaannnnngaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcggccctcttcgctattacgccagaattcgagctcggtacccctgagtctaggtcgga Mask Tandem Repeat Region ================================================ tccgacctagactcaggggtaccgagctcgaattctggcgtaatagcgaagagggccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcnnnnnttaattaattaannnnngcaaagaaaacagagattgatgcgaccatggcttaacagcagaggaaaacctagcagctarggagaagctagggcaaaagcagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Find is-nt database================================================ Query_seq: SMT_2006:SMT_2007|SMT_2006:SMT_2007:hypothetical protein predicted by Glimmer/Critica:hypothetical protein:->->:1926864..1927652 789 tccgacctagactcaggggtaccgagctcgaattctggcgtaatagcgaagagggccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcnnnnnttaattaattaannnnngcaaagaaaacagagattgatgcgaccatggcttaacagcagaggaaaacctagcagctarggagaagctagggcaaaagcagatgcagctaagaaagcaatcgatggagctgatacagatattaaggtgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagamccagttagaatcagaagctaaaaaagctgtagatgaagcactaaaggcaaaagaagcagaaatkgatgcgaataatgacttaacagcagaagaaaaattagcagctaaggaggaagctaaagctaaagccgatgcagctaagaaagcaatcgatgaagctgatacagatattaaggttgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagaaccagttaagaaatcagaggctaaaaaagcaattgatgcagctctagagaagaaaatagaagaaatcgatgctcgagatgatttaacaactgaagagaaaactgcagcaaaagctgaagtagaaaataaagctaatgaggcaaaagacgcaatcgatgcagcagatacagacattaaagtagatgtaaatagtgtatctggtttaagtgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_2006:SMT_2007|SMT_2006:SMT_2007:hypothetical protein predicted by Glimmer/Critica:hypothetical protein:->->:1926864..1927652 789 tccgacctagactcaggggtaccgagctcgaattctggcgtaatagcgaagagggccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcnnnnnttaattaattaannnnngcaaagaaaacagagattgatgcgaccatggcttaacagcagaggaaaacctagcagctarggagaagctagggcaaaagcagatgcagctaagaaagcaatcgatggagctgatacagatattaaggtgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagamccagttagaatcagaagctaaaaaagctgtagatgaagcactaaaggcaaaagaagcagaaatkgatgcgaataatgacttaacagcagaagaaaaattagcagctaaggaggaagctaaagctaaagccgatgcagctaagaaagcaatcgatgaagctgatacagatattaaggttgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagaaccagttaagaaatcagaggctaaaaaagcaattgatgcagctctagagaagaaaatagaagaaatcgatgctcgagatgatttaacaactgaagagaaaactgcagcaaaagctgaagtagaaaataaagctaatgaggcaaaagacgcaatcgatgcagcagatacagacattaaagtagatgtaaatagtgtatctggtttaagtgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_2006:SMT_2007|SMT_2006:SMT_2007:hypothetical protein predicted by Glimmer/Critica:hypothetical protein:->->:1926864..1927652 789 tccgacctagactcaggggtaccgagctcgaattctggcgtaatagcgaagagggccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcnnnnnttaattaattaannnnngcaaagaaaacagagattgatgcgaccatggcttaacagcagaggaaaacctagcagctarggagaagctagggcaaaagcagatgcagctaagaaagcaatcgatggagctgatacagatattaaggtgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagamccagttagaatcagaagctaaaaaagctgtagatgaagcactaaaggcaaaagaagcagaaatkgatgcgaataatgacttaacagcagaagaaaaattagcagctaaggaggaagctaaagctaaagccgatgcagctaagaaagcaatcgatgaagctgatacagatattaaggttgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagaaccagttaagaaatcagaggctaaaaaagcaattgatgcagctctagagaagaaaatagaagaaatcgatgctcgagatgatttaacaactgaagagaaaactgcagcaaaagctgaagtagaaaataaagctaatgaggcaaaagacgcaatcgatgcagcagatacagacattaaagtagatgtaaatagtgtatctggtttaagtgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 4 Min: 3 Max: 148 Len: 146 Subject: UniRef90_A0ZLG1 Cluster: Beta-D-galactosidase; n=1; Nodularia spumigena CCY9414|Rep: Beta-D-galactosidase - Nodularia spumigena CCY9414 HSP 1 e-value: 7.0E-13 bit: 77.0 Len: 111 Query Start:3 Query End:113 Subject Strand: null Subject Start: 36 Subject End: 72 .. R P R L R G T E L E F W R N S E E G R T D R P S Q Q L R S L N G E W R L M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... .. R P R L T G T E L E F W R N S E E A R T D R P S Q Q L R S L N G E W R L M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q6QI34 Cluster: LRRGT00174; n=1; Rattus norvegicus|Rep: LRRGT00174 - Rattus norvegicus (Rat) HSP 1 e-value: 5.0E-10 bit: 67.4 Len: 126 Query Start:21 Query End:146 Subject Strand: null Subject Start: 312 Subject End: 353 .................... N T A Q M R K E K I P H Q A P F A I Q A A Q L L G R A I G A A L F A I T P E F E L G ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... .................... N T A Q M R K E K I T H Q A P F A I Q D A H M L G R A I G A G L F A I T P A G E R G ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_UPI0000ECAFAB Cluster: UPI0000ECAFAB related cluster; n=1; Gallus gallus|Rep: UPI0000ECAFAB UniRef100 entry - Gallus gallus HSP 1 e-value: 3.0E-7 bit: 45.1 Len: 57 Query Start:92 Query End:148 Subject Strand: null Subject Start: 50 Subject End: 68 ........................................................................................... E W R M A P D A V F S P Y A S V R Y F ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. ........................................................................................... E W R M A P D A V F S P Y A S V R Y F ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 3.0E-7 bit: 33.1 Len: 63 Query Start:48 Query End:110 Subject Strand: null Subject Start: 36 Subject End: 53 ............................................... E E G R T D R P S Q Q L R S L N G E W R L ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ............................................... E E A R T D R P S Q Q L R S - - - E W R M ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_O21999 Cluster: LacZ-alpha; n=1; Cloning vector pAL-Z|Rep: LacZ-alpha - Cloning vector pAL-Z HSP 1 e-value: 4.0E-7 bit: 57.8 Len: 105 Query Start:36 Query End:140 Subject Strand: null Subject Start: 70 Subject End: 106 ................................... W R N S E E G R T D R P S Q Q L R S L N G E W R - - L M R Y F L L T H L C ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ................................... W R N S E E A R T D R P S Q Q L R S L N G E W R D K L A L Y A L M Q F L C ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... tcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttaattaattaannnnngcaaagaaaacagagattgatgcgaccatggcttaacagcagaggaaaacctagcagctarggagaagctagggcaaaagcagatgcagctaagaaagcaatcgatggagctgatacagatattaaggtgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagamccagttagaatcagaagctaaaaaagctgtagatgaagcactaaaggcaaaagaagcagaaatkgatgcgaataatgacttaacagcagaagaaaaattagcagctaaggaggaagctaaagctaaagccgatgcagctaagaaagcaatcgatgaagctgatacagatattaaggttgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagaaccagttaagaaatcagaggctaaaaaagcaattgatgcagctctagagaagaaaatagaagaaatcgatgctcgagatgatttaacaactgaagagaaaactgcagcaaaagctgaagtagaaaataaagctaatgaggcaaaagacgcaatcgatgcagcagatacagacattaaagtagatgtaaatagtgtatctggtttaagtgat Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 334 End: 789 ............................................................................................................................................................................................................................................................................................................................................. M H L V Q X Q L E S E A K K A V D E A L K A K E A E X D A N N D L T A E E K L A A K E E A K A K A D A A K K A I D E A D Protein_Len: 35 Strand: - Start: 664 End: 768 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... C S F L F S C C F S F Y F I F S I L C F V C D I C C I C V N F Y I Y M ..................... Protein_Len: 60 Strand: - Start: 339 End: 752 .................................................................................................................................................................................................................................................................................................................................................. G S G T L F D S A L F A I S A A R S F F I S S I S A R S S K V V S S F V A A F A S T S F L A L S A F S A I S A A S V S M ..................................... Protein_Len: 60 Strand: - Start: 157 End: 339 ............................................................................................................................................................ I L X X L S F L S Q H S W P K V A S S F R A A X S F S P C F C I C S L F C D I S S I C I N L H H L Y Y I Q N L H H L N M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 52 Strand: - Start: 3 End: 158 .. S R S E P T G L E F E P T I A F L A A G I A R G L L Q A A Q I A F P A Q H P I K E K R M Q A T N X X X M ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 48 Strand: - Start: 2 End: 145 . R G L S L P V S S S N Q R L L S S P R V S R G E W C N R L R F P S H R R I R Y K R R V C R H P M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... tcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttaattaattaannnnngcaaagaaaacagagattgatgcgaccatggcttaacagcagaggaaaacctagcagctarggagaagctagggcaaaagcagatgcagctaagaaagcaatcgatggagctgatacagatattaaggtgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagamccagttagaatcagaagctaaaaaagctgtagatgaagcactaaaggcaaaagaagcagaaatkgatgcgaataatgacttaacagcagaagaaaaattagcagctaaggaggaagctaaagctaaagccgatgcagctaagaaagcaatcgatgaagctgatacagatattaaggttgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagaaccagttaagaaatcagaggctaaaaaagcaattgatgcagctctagagaagaaaatagaagaaatcgatgctcgagatgatttaacaactgaagagaaaactgcagcaaaagctgaagtagaaaataaagctaatgaggcaaaagacgcaatcgatgcagcagatacagacattaaagtagatgtaaatagtgtatctggtttaagtgat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttaattaattaannnnngcaaagaaaacagagattgatgcgaccatggcttaacagcagaggaaaacctagcagctarggagaagctagggcaaaagcagatgcagctaagaaagcaatcgatggagctgatacagatattaaggtgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagamccagttagaatcagaagctaaaaaagctgtagatgaagcactaaaggcaaaagaagcagaaatkgatgcgaataatgacttaacagcagaagaaaaattagcagctaaggaggaagctaaagctaaagccgatgcagctaagaaagcaatcgatgaagctgatacagatattaaggttgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagaaccagttaagaaatcagaggctaaaaaagcaattgatgcagctctagagaagaaaatagaagaaatcgatgctcgagatgatttaacaactgaagagaaaactgcagcaaaagctgaagtagaaaataaagctaatgaggcaaaagacgcaatcgatgcagcagatacagacattaaagtagatgtaaatagtgtatctggtttaagtgat Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 78 HP_score: -4.0 Tail_Score: -5.2291 Start: 448 End: 458 Full_Region: gctttagcttcctcc ttag ctg ctaa tttttcttctgctgt ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................ttagctgctaa........................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 73 HP_score: -5.4 Tail_Score: -4.58419 Start: 644 End: 654 Full_Region: agttgttaaatcatc tcga gca tcga tttcttctattttct ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tcgagcatcga....................................................................................................................................... TransTerm Strand: - Conf: 73 HP_score: -5.5 Tail_Score: -4.7033 Start: 680 End: 697 Full_Region: tagctttattttcta cttcagc tttt gctgcag ttttctcttcagttg .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cttcagcttttgctgcag............................................................................................ Find igs database================================================ Query_seq: SMT_2006:SMT_2007|SMT_2006:SMT_2007:hypothetical protein predicted by Glimmer/Critica:hypothetical protein:->->:1926864..1927652 789 tcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttaattaattaannnnngcaaagaaaacagagattgatgcgaccatggcttaacagcagaggaaaacctagcagctarggagaagctagggcaaaagcagatgcagctaagaaagcaatcgatggagctgatacagatattaaggtgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagamccagttagaatcagaagctaaaaaagctgtagatgaagcactaaaggcaaaagaagcagaaatkgatgcgaataatgacttaacagcagaagaaaaattagcagctaaggaggaagctaaagctaaagccgatgcagctaagaaagcaatcgatgaagctgatacagatattaaggttgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagaaccagttaagaaatcagaggctaaaaaagcaattgatgcagctctagagaagaaaatagaagaaatcgatgctcgagatgatttaacaactgaagagaaaactgcagcaaaagctgaagtagaaaataaagctaatgaggcaaaagacgcaatcgatgcagcagatacagacattaaagtagatgtaaatagtgtatctggtttaagtgat Intra-Species Hit: Count: 2 Min: 154 Max: 789 Len: 636 Subject: smit_SMT_2006_SMT_2007|hypothetical protein predicted by Glimmer/Critica:hypothetical protein|POSITIVE:POSITIVE|[1926864,1927652]|789 HSP 1 e-value: 0.0 bit: 1223.0 Len: 636 Query Start:154 Query End:789 Subject Strand: POSITIVE Subject Start: 154 Subject End: 789 .........................................................................................................................................................ttaattaattaannnnngcaaagaaaacagagattgatgcgaccatggcttaacagcagaggaaaacctagcagctarggagaagctagggcaaaagcagatgcagctaagaaagcaatcgatggagctgatacagatattaaggtgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagamccagttagaatcagaagctaaaaaagctgtagatgaagcactaaaggcaaaagaagcagaaatkgatgcgaataatgacttaacagcagaagaaaaattagcagctaaggaggaagctaaagctaaagccgatgcagctaagaaagcaatcgatgaagctgatacagatattaaggttgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagaaccagttaagaaatcagaggctaaaaaagcaattgatgcagctctagagaagaaaatagaagaaatcgatgctcgagatgatttaacaactgaagagaaaactgcagcaaaagctgaagtagaaaataaagctaatgaggcaaaagacgcaatcgatgcagcagatacagacattaaagtagatgtaaatagtgtatctggtttaagtgat .........................................................................................................................................................ttaattaattaannnnngcaaagaaaacagagattgatgcgaccatggcttaacagcagaggaaaacctagcagctarggagaagctagggcaaaagcagatgcagctaagaaagcaatcgatggagctgatacagatattaaggtgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagamccagttagaatcagaagctaaaaaagctgtagatgaagcactaaaggcaaaagaagcagaaatkgatgcgaataatgacttaacagcagaagaaaaattagcagctaaggaggaagctaaagctaaagccgatgcagctaagaaagcaatcgatgaagctgatacagatattaaggttgatgtaaatagtatatctggtttaagtgatgtaaattcatttagtccagaaccagttaagaaatcagaggctaaaaaagcaattgatgcagctctagagaagaaaatagaagaaatcgatgctcgagatgatttaacaactgaagagaaaactgcagcaaaagctgaagtagaaaataaagctaatgaggcaaaagacgcaatcgatgcagcagatacagacattaaagtagatgtaaatagtgtatctggtttaagtgat Subject: smit_SMT_1940_SMT_1941|hypothetical protein predicted by Glimmer/Critica:hypothetical protein|POSITIVE:POSITIVE|[1877167,1877330]|164 HSP 1 e-value: 7.0E-88 bit: 326.0 Len: 164 Query Start:626 Query End:789 Subject Strand: POSITIVE Subject Start: 1 Subject End: 164 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................agaagaaaatagaagaaatcgatgctcgagatgatttaacaactgaagagaaaactgcagcaaaagctgaagtagaaaataaagctaatgaggcaaaagacgcaatcgatgcagcagatacagacattaaagtagatgtaaatagtgtatctggtttaagtgat .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................agaagaaaatagaagaaatcgatgctcgagatgatttaacaactgaagagaaaactgcagcaaaagctgaagtagaaaataaagctaatgaggcaaaagacgcaatcgatgcagcagatacagacattaaagtagatgtaaatagtgtatctggtttaagtgat HSP 2 e-value: 4.0E-13 bit: 78.0 Len: 63 Query Start:496 Query End:558 Subject Strand: POSITIVE Subject Start: 102 Subject End: 164 ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gcaatcgatgaagctgatacagatattaaggttgatgtaaatagtatatctggtttaagtgat....................................................................................................................................................................................................................................... ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gcaatcgatgcagcagatacagacattaaagtagatgtaaatagtgtatctggtttaagtgat....................................................................................................................................................................................................................................... HSP 3 e-value: 1.0E-10 bit: 70.1 Len: 62 Query Start:268 Query End:329 Subject Strand: POSITIVE Subject Start: 102 Subject End: 164 ...........................................................................................................................................................................................................................................................................gcaatcgatggagctgatacagatattaaggt-gatgtaaatagtatatctggtttaagtgat............................................................................................................................................................................................................................................................................................................................................................................................................................................................................ ...........................................................................................................................................................................................................................................................................gcaatcgatgcagcagatacagacattaaagtagatgtaaatagtgtatctggtttaagtgat............................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAAUUAAUUAANNNNNGCAAAGAAAACAGAGAUUGAUGCGACCAUGGCUUAACAGCAGAGGAAAACCUAGCAGCUARGGAGAAGCUAGGGCAAAAGCAGAUGCAGCUAAGAAAGCAAUCGAUGGAGCUGAUACAGAUAUUAAGGUGAUGUAAAUAGUAUAUCUGGUUUAAGUGAUGUAAAUUCAUUUAGUCCAGAMCCAGUUAGAAUCAGAAGCUAAAAAAGCUGUAGAUGAAGCACUAAAGGCAAAAGAAGCAGAAAUKGAUGCGAAUAAUGACUUAACAGCAGAAGAAAAAUUAGCAGCUAAGGAGGAAGCUAAAGCUAAAGCCGAUGCAGCUAAGAAAGCAAUCGAUGAAGCUGAUACAGAUAUUAAGGUUGAUGUAAAUAGUAUAUCUGGUUUAAGUGAUGUAAAUUCAUUUAGUCCAGAACCAGUUAAGAAAUCAGAGGCUAAAAAAGCAAUUGAUGCAGCUCUAGAGAAGAAAAUAGAAGAAAUCGAUGCUCGAGAUGAUUUAACAACUGAAGAGAAAACUGCAGCAAAAGCUGAAGUAGAAAAUAAAGCUAAUGAGGCAAAAGACGCAAUCGAUGCAGCAGAUACAGACAUUAAAGUAGAUGUAAAUAGUGUAUCUGGUUUAAGUGAU ........................((((....(((((((((....((.((((..(((.........((((((..((....))..)))))).............(((((.....(((..(((((..(((((.((((..(((....))).)))).........(((((.((((((((.......)))))))).)))))..)))))...))).)).)))......(((((((.......((((...(((......(((........)))...(((((.((((.((((..........(((((((((........))))...)))))...((((...(((.....))).)))).....)))).)).)).))))).......)))...))))...(((((.((((((((.......)))))))).)))))..(((((...((((((....((.....((((.(((((....(((((..........))).))..)))))))))..)).))))))...))))).........)))))))...)))))..............)))..)))).)).....))).))))))....((((((((.(((((......)))))......))))))))))))....... (-142.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCACUUAAACCAGAUACACUAUUUACAUCUACUUUAAUGUCUGUAUCUGCUGCAUCGAUUGCGUCUUUUGCCUCAUUAGCUUUAUUUUCUACUUCAGCUUUUGCUGCAGUUUUCUCUUCAGUUGUUAAAUCAUCUCGAGCAUCGAUUUCUUCUAUUUUCUUCUCUAGAGCUGCAUCAAUUGCUUUUUUAGCCUCUGAUUUCUUAACUGGUUCUGGACUAAAUGAAUUUACAUCACUUAAACCAGAUAUACUAUUUACAUCAACCUUAAUAUCUGUAUCAGCUUCAUCGAUUGCUUUCUUAGCUGCAUCGGCUUUAGCUUUAGCUUCCUCCUUAGCUGCUAAUUUUUCUUCUGCUGUUAAGUCAUUAUUCGCAUCMAUUUCUGCUUCUUUUGCCUUUAGUGCUUCAUCUACAGCUUUUUUAGCUUCUGAUUCUAACUGGKUCUGGACUAAAUGAAUUUACAUCACUUAAACCAGAUAUACUAUUUACAUCACCUUAAUAUCUGUAUCAGCUCCAUCGAUUGCUUUCUUAGCUGCAUCUGCUUUUGCCCUAGCUUCUCCYUAGCUGCUAGGUUUUCCUCUGCUGUUAAGCCAUGGUCGCAUCAAUCUCUGUUUUCUUUGCNNNNNUUAAUUAAUUAA ...........((((((((......((((........)))).))))))))..(((..(((((((.((...((.....((((..............(((((......((((((......((((..((((((.......((((((((((.................((((((..(((.....)))..))))))...(((((.........((((((((..(((.(((.......))).)))..)))))................)))...........)))))....))))).)))))..(((((.((...((....(((....)))....))...)).)))))..........(((((.(((.(((((...(((.................)))...)))))))).....)))))...))))))..)))).......(((..((((..(((.(((.......))).)))...(((((((..................)))))))..))))..)))..))))))......))))).............((((((..((....))..)))))).........))))....))...)).))))...)))..))).......................... (-112.44) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 471 290 241 222 176 smit:SMT_2109|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1999099..1999329_NEGATIVE 345 99 54 71 25 smit:SMT_147|5end_K02027_multiple_sugar_transport_system_substrate-binding_protein_97406..97636_POSITIVE 345 99 54 71 25 smit:SMT_119|5end_K02027_multiple_sugar_transport_system_substrate-binding_protein_70457..70687_POSITIVE 330 327 283 56 4 smit:SMT_2083|5end_K06147_ATP-binding_cassette,_subfamily_B,_bacterial_1982411..1982641_POSITIVE 327 130 87 54 9 smit:SMT_1586|5end_K07177_PDZ_domain-containing_protein_1575714..1575944_POSITIVE 316 113 71 72 27 smit:SMT_274|5end_ABC_transporter_substrate-binding_protein_-_oligopeptide_transport_215221..215451_POSITIVE 314 90 41 159 110 smit:SMT_387|5end_hypothetical_protein_predicted_by_Glimmer/Critica_320937..321167_POSITIVE 312 105 63 80 36 smit:SMT_1178|5end_choline_binding_protein_A_1136638..1136868_NEGATIVE 309 90 43 201 148 smit:SMT_1897|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1848858..1849088_NEGATIVE 304 86 41 43 1 smit:SMT_1369|5end_alanine_dehydrogenase_1348280..1348510_POSITIVE 301 337 305 228 190 smit:SMT_1477|5end_K02077_zinc/manganese_transport_system_substrate-binding_protein_1460376..1460606_NEGATIVE 298 254 231 42 17 smit:SMT_1782|5end_hypothetical_protein_1759520..1759750_POSITIVE 298 75 31 99 57 smit:SMT_712|5end_alcohol_dehydrogenase,_zinc-containing_620381..620611_NEGATIVE 293 73 37 125 78 smit:SMT_1855|5end_K03650_tRNA_modification_GTPase_1819711..1819941_POSITIVE 291 77 32 196 150 smit:SMT_317|5end_K03625_N_utilization_substance_protein_B_257900..258130_NEGATIVE 291 109 61 54 12 smit:SMT_155|5end_hypothetical_protein_102704..102934_NEGATIVE 289 128 84 71 18 smit:SMT_1923|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1866021..1866251_POSITIVE 288 87 43 195 143 smit:SMT_1739|5end_hypothetical_protein_1721263..1721493_POSITIVE 288 90 54 68 30 smit:SMT_1449|5end_K08591_putative_membrane_protein_1431937..1432167_POSITIVE 288 110 65 223 186 smit:SMT_832|5end_hypothetical_protein_775104..775334_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 327 130 87 70 25 smit:SMT_1585|3end_phosphopantetheine_adenylyltransferase_1575810..1575960_POSITIVE 323 130 81 60 7 smit:SMT_890|3end_K02029_polar_amino_acid_transport_system_permease_protein_837692..837842_POSITIVE 314 90 41 144 95 smit:SMT_386|3end_branched-chain_amino_acid_transport_system_II_carrier_protein_321002..321152_POSITIVE 304 356 324 68 35 smit:SMT_2057|3end_cell_division_protein_FtsH_1966897..1967047_NEGATIVE 294 75 38 47 5 smit:SMT_140|3end_NUDIX_family_hydrolase_91498..91648_POSITIVE 292 72 31 149 99 smit:SMT_1857|3end_hypothetical_protein_1821145..1821295_POSITIVE 288 110 65 144 107 smit:SMT_833|3end_hypothetical_protein_predicted_by_Glimmer/Critica_775183..775333_NEGATIVE 284 350 301 72 30 smit:SMT_794|3end_hypothetical_protein_703245..703395_NEGATIVE 283 359 311 59 8 smit:SMT_799|3end_hypothetical_protein_707296..707446_NEGATIVE 280 120 71 71 22 smit:SMT_1540|3end_hypothetical_protein_1524307..1524457_NEGATIVE 278 328 287 85 36 smit:SMT_1524|3end_peptidase_T_1505867..1506017_POSITIVE 278 350 304 64 15 smit:SMT_847|3end_thioredoxin_reductase_793848..793998_POSITIVE 270 106 64 72 33 smit:SMT_481|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_417641..417791_NEGATIVE 268 608 571 39 1 smit:SMT_2092|3end_malolactic_enzyme_1987040..1987190_NEGATIVE 265 337 294 62 9 smit:SMT_1774|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1750151..1750301_NEGATIVE 264 359 311 61 10 smit:SMT_1809|3end_MutT/NUDIX_family_protein_1785134..1785284_POSITIVE 259 110 73 45 7 smit:SMT_2068|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1974183..1974333_NEGATIVE 259 83 43 79 38 smit:SMT_272|3end_hypothetical_protein_211831..211981_NEGATIVE 255 88 44 46 2 smit:SMT_1865|3end_D-alanyl_carrier_protein_1827377..1827527_POSITIVE 255 344 301 61 10 smit:SMT_1367|3end_K02237_competence_protein_ComEA_1346872..1347022_NEGATIVE