Origin IGS: aaaattgactttcaattgaatttttggtagaatagctgtattaggtctcatagctcagctggatagagcattcgccttctaagcgaacggtcgcaggtcttccagcnnnnnttaattaattaannnnntgttggcgggtgtcggggctggcttaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttaagccagccccgacacccgccaacannnnnttaattaattaannnnngctggaagacctgcgaccgttcgcttagaaggcgaatgctctatccagctgagctatgagacctaatacagctattctaccaaaaattcaattgaaagtcaatttt Mask Tandem Repeat Region ================================================ aaaattgactttcaattgaatttttggtagaatagctgtattaggtctcatagctcagctggatagagcattcgccttctaagcgaacggtcgcaggtcttccagcnnnnnttaattaattaannnnntgttggcgggtgtcggggctggcttaa Find is-nt database================================================ Query_seq: SMT_2004:SMT_2005|SMT_2004:SMT_2005:K00783 hypothetical protein:pullulanase, type I:-><-:1925550..1925704 155 aaaattgactttcaattgaatttttggtagaatagctgtattaggtctcatagctcagctggatagagcattcgccttctaagcgaacggtcgcaggtcttccagcnnnnnttaattaattaannnnntgttggcgggtgtcggggctggcttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_2004:SMT_2005|SMT_2004:SMT_2005:K00783 hypothetical protein:pullulanase, type I:-><-:1925550..1925704 155 aaaattgactttcaattgaatttttggtagaatagctgtattaggtctcatagctcagctggatagagcattcgccttctaagcgaacggtcgcaggtcttccagcnnnnnttaattaattaannnnntgttggcgggtgtcggggctggcttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_2004:SMT_2005|SMT_2004:SMT_2005:K00783 hypothetical protein:pullulanase, type I:-><-:1925550..1925704 155 aaaattgactttcaattgaatttttggtagaatagctgtattaggtctcatagctcagctggatagagcattcgccttctaagcgaacggtcgcaggtcttccagcnnnnnttaattaattaannnnntgttggcgggtgtcggggctggcttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaattgactttcaattgaatttttggtagaatagctgtattaggtctcatagctcagctggatagagcattcgccttctaagcgaacggtcgcaggtcttccagcnnnnnttaattaattaannnnntgttggcgggtgtcggggctggcttaa Predict ORF larger than 30AA ================================================ aaaattgactttcaattgaatttttggtagaatagctgtattaggtctcatagctcagctggatagagcattcgccttctaagcgaacggtcgcaggtcttccagcnnnnnttaattaattaannnnntgttggcgggtgtcggggctggcttaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 90 Start: 4 End: 33 ...ATTGACTTTCAATTGAATTTTTGGTAGAAT.......................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaattgactttcaattgaatttttggtagaatagctgtattaggtctcatagctcagctggatagagcattcgccttctaagcgaacggtcgcaggtcttccagcnnnnnttaattaattaannnnntgttggcgggtgtcggggctggcttaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_2004:SMT_2005|SMT_2004:SMT_2005:K00783 hypothetical protein:pullulanase, type I:-><-:1925550..1925704 155 aaaattgactttcaattgaatttttggtagaatagctgtattaggtctcatagctcagctggatagagcattcgccttctaagcgaacggtcgcaggtcttccagcnnnnnttaattaattaannnnntgttggcgggtgtcggggctggcttaa Intra-Species Hit: Count: 1 Min: 1 Max: 155 Len: 155 Subject: smit_SMT_2004_SMT_2005|K00783 hypothetical protein:pullulanase, type I|POSITIVE:NEGATIVE|[1925550,1925704]|155 HSP 1 e-value: 5.0E-65 bit: 248.0 Len: 155 Query Start:1 Query End:155 Subject Strand: POSITIVE Subject Start: 1 Subject End: 155 aaaattgactttcaattgaatttttggtagaatagctgtattaggtctcatagctcagctggatagagcattcgccttctaagcgaacggtcgcaggtcttccagcnnnnnttaattaattaannnnntgttggcgggtgtcggggctggcttaa aaaattgactttcaattgaatttttggtagaatagctgtattaggtctcatagctcagctggatagagcattcgccttctaagcgaacggtcgcaggtcttccagcnnnnnttaattaattaannnnntgttggcgggtgtcggggctggcttaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAUUGACUUUCAAUUGAAUUUUUGGUAGAAUAGCUGUAUUAGGUCUCAUAGCUCAGCUGGAUAGAGCAUUCGCCUUCUAAGCGAACGGUCGCAGGUCUUCCAGCNNNNNUUAAUUAAUUAANNNNNUGUUGGCGGGUGUCGGGGCUGGCUUAA ..(((((.....)))))........(((.((((.(((.((((.((.((........)))).)))).))))))))))...(((((...(((((.((..(((.(((((.....((((....))))......))))).)))..).).)))))))))). (-32.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAAGCCAGCCCCGACACCCGCCAACANNNNNUUAAUUAAUUAANNNNNGCUGGAAGACCUGCGACCGUUCGCUUAGAAGGCGAAUGCUCUAUCCAGCUGAGCUAUGAGACCUAAUACAGCUAUUCUACCAAAAAUUCAAUUGAAAGUCAAUUUU .............(((...................................(((.(((.....((.((((((((.....)))))))).)).....(((((...(((........))))))))..))).)))....(((....))).)))...... (-25.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 485 150 129 215 194 smit:SMT_1887|5end_LicC_protein_1841445..1841675_POSITIVE 325 105 64 52 6 smit:SMT_1729|5end_exodeoxyribonuclease_VII_small_subunit_1714566..1714796_POSITIVE 316 89 41 58 9 smit:SMT_1024|5end_transcriptional_regulator,_MarR_family_959404..959634_NEGATIVE 315 99 57 203 159 smit:SMT_1982|5end_conserved_hypothetical_protein_1913036..1913266_POSITIVE 315 99 57 191 147 smit:SMT_1691|5end_putative_deoxyribonuclease_1674978..1675208_POSITIVE 315 99 57 216 172 smit:SMT_1156|5end_K07485_transposase_1099938..1100168_POSITIVE 314 100 52 62 26 smit:SMT_274|5end_ABC_transporter_substrate-binding_protein_-_oligopeptide_transport_215221..215451_POSITIVE 313 120 71 174 131 smit:SMT_1943|5end_alpha/beta_hydrolase_fold_1878968..1879198_NEGATIVE 304 100 52 69 21 smit:SMT_2089|5end_dTDP-4-dehydrorhamnose_3,5-epimerase_related_enzyme_1984987..1985217_POSITIVE 304 100 52 69 21 smit:SMT_291|5end_dTDP-4-dehydrorhamnose_3,5-epimerase_234197..234427_POSITIVE 298 106 64 130 67 smit:SMT_1958|5end_tryptophan_synthase,_beta_subunit_1890980..1891210_POSITIVE 297 120 71 146 98 smit:SMT_1681|5end_D-alanine-activating_enzyme_1664184..1664414_POSITIVE 297 87 42 63 9 smit:SMT_765|5end_hypothetical_protein_672620..672850_NEGATIVE 296 100 53 51 14 smit:SMT_1719|5end_DNA_mismatch_repair_protein_1706842..1707072_NEGATIVE 294 98 52 50 7 smit:SMT_1213|5end_fatty_acid/phospholipid_synthesis_protein_PlsX_1172655..1172885_NEGATIVE 292 89 44 50 2 smit:SMT_1742|5end_sodium-dependent_phosphate_transporter_1723553..1723783_POSITIVE 290 98 52 213 165 smit:SMT_991|5end_hypothetical_protein_934425..934655_NEGATIVE 286 99 51 188 130 smit:SMT_2002|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1924405..1924635_NEGATIVE 286 99 51 227 169 smit:SMT_1966|5end_DNA_polymerase_III_alpha_subunit_1897934..1898164_POSITIVE 286 99 51 188 130 smit:SMT_1942|5end_zinc_metalloprotease_ZmpB,_truncated_1877882..1878112_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 325 105 64 74 28 smit:SMT_1728|3end_exodeoxyribonuclease_VII_large_subunit_1714668..1714818_POSITIVE 313 120 71 67 24 smit:SMT_1944|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1879075..1879225_NEGATIVE 304 100 52 68 20 smit:SMT_2088|3end_glucose-1-phosphate_thymidylyltransferase_1985066..1985216_POSITIVE 304 99 53 55 1 smit:SMT_1115|3end_K02245_competence_protein_ComGC_1052473..1052623_NEGATIVE 304 100 52 68 20 smit:SMT_290|3end_glucose-1-phosphate_thymidylyltransferase_234276..234426_POSITIVE 302 106 61 58 1 smit:SMT_1729|3end_exodeoxyribonuclease_VII_small_subunit_1714858..1715008_POSITIVE 297 120 71 55 7 smit:SMT_1680|3end_hypothetical_protein_1664173..1664323_POSITIVE 294 98 52 53 10 smit:SMT_1214|3end_K03584_DNA_repair_protein_RecO_(recombination_protein_O)_1172652..1172802_NEGATIVE 293 106 63 132 73 smit:SMT_1530|3end_K07456_DNA_mismatch_repair_protein_MutS2_1513010..1513160_NEGATIVE 292 100 54 48 4 smit:SMT_762|3end_cation-transporting_ATPase,_E1-E2_family_protein_667368..667518_NEGATIVE 290 98 52 109 61 smit:SMT_992|3end_hypothetical_protein_934529..934679_NEGATIVE 286 100 52 76 24 smit:SMT_1941|3end_hypothetical_protein_1877663..1877813_POSITIVE 284 99 51 62 22 smit:SMT_1874|3end_hypothetical_protein_1832623..1832773_NEGATIVE 284 84 42 142 107 smit:SMT_424|3end_transcriptional_regulatory_protein_368108..368258_POSITIVE 282 100 53 58 8 smit:SMT_1812|3end_K07485_transposase_1787193..1787343_POSITIVE 282 106 61 65 9 smit:SMT_1078|3end_folylpolyglutamate_synthase_1015391..1015541_NEGATIVE 282 100 55 137 72 smit:SMT_492|3end_branched-chain_amino_acid_ABC_transporter,_ATP-binding_protein_427217..427367_POSITIVE 277 106 62 73 30 smit:SMT_1695|3end_ribulose-phosphate_3-epimerase_1678299..1678449_POSITIVE 275 100 58 109 56 smit:SMT_2112|3end_transcription-repair_coupling_factor_2000373..2000523_NEGATIVE 275 100 53 115 68 smit:SMT_1396|3end_uracil-DNA_glycosylase_1372797..1372947_POSITIVE