Origin IGS: actcaggggtaccgagctcgaattctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgnnnnnttaattaattaannnnngaagcggaagagcgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcgggttgcgtattgggcgctcttccgcttcnnnnnttaattaattaannnnncagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagaattcgagctcggtacccctgagt Mask Tandem Repeat Region ================================================ actcaggggtaccgagctcgaattctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgnnnnnttaattaattaannnnngaagcggaagagcgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Find is-nt database================================================ Query_seq: SMT_2001:SMT_2002|SMT_2001:SMT_2002:membrane protease protein family:hypothetical protein predicted by Glimmer/Critica:-><-:1923950..1924234 285 actcaggggtaccgagctcgaattctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgnnnnnttaattaattaannnnngaagcggaagagcgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_2001:SMT_2002|SMT_2001:SMT_2002:membrane protease protein family:hypothetical protein predicted by Glimmer/Critica:-><-:1923950..1924234 285 actcaggggtaccgagctcgaattctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgnnnnnttaattaattaannnnngaagcggaagagcgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_2001:SMT_2002|SMT_2001:SMT_2002:membrane protease protein family:hypothetical protein predicted by Glimmer/Critica:-><-:1923950..1924234 285 actcaggggtaccgagctcgaattctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgnnnnnttaattaattaannnnngaagcggaagagcgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 3 Min: 2 Max: 160 Len: 159 Subject: UniRef90_A0ZLG1 Cluster: Beta-D-galactosidase; n=1; Nodularia spumigena CCY9414|Rep: Beta-D-galactosidase - Nodularia spumigena CCY9414 HSP 1 e-value: 6.0E-12 bit: 72.0 Len: 102 Query Start:2 Query End:103 Subject Strand: null Subject Start: 39 Subject End: 72 . L R G T E L E F W R N S E E A R T D R P S Q Q L R S L N G E W R L M ...................................................................................................................................................................................... . L T G T E L E F W R N S E E A R T D R P S Q Q L R S L N G E W R L M ...................................................................................................................................................................................... Subject: UniRef90_Q6QI34 Cluster: LRRGT00174; n=1; Rattus norvegicus|Rep: LRRGT00174 - Rattus norvegicus (Rat) HSP 1 e-value: 2.0E-9 bit: 63.9 Len: 117 Query Start:11 Query End:127 Subject Strand: null Subject Start: 315 Subject End: 353 .......... Q M R K E K I P H Q A P F A I Q A A Q L L G R A I G A G L F A I T P E F E L G .............................................................................................................................................................. .......... Q M R K E K I T H Q A P F A I Q D A H M L G R A I G A G L F A I T P A G E R G .............................................................................................................................................................. Subject: UniRef90_O21999 Cluster: LacZ-alpha; n=1; Cloning vector pAL-Z|Rep: LacZ-alpha - Cloning vector pAL-Z HSP 1 e-value: 3.0E-7 bit: 56.6 Len: 135 Query Start:26 Query End:160 Subject Strand: null Subject Start: 70 Subject End: 116 ......................... W R N S E E A R T D R P S Q Q L R S L N G E W R - - L M R Y F L L T H L X X * L I X X X S G R ............................................................................................................................. ......................... W R N S E E A R T D R P S Q Q L R S L N G E W R D K L A L Y A L M Q F L C A P V L G A L S D R ............................................................................................................................. annnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngcgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Predict ORF larger than 30AA ================================================ Protein_Len: 48 Strand: + Start: 140 End: 283 ........................................................................................................................................... M X X X S G R A P N T Q P A S P R A L A D S L M Q S L H A C R S T L E D P P V L G W S P R S G N .. Protein_Len: 42 Strand: - Start: 144 End: 269 ............................................................................................................................................... X X F R F L A W Y A V R R E G R T P R N M L A S S A H R C T S E L P D G Q A L I T M ................ Protein_Len: 50 Strand: - Start: 136 End: 285 ....................................................................................................................................... N I L X X L P L A G L V C G A E G R A N A S E N I C L K C A Q L D V R S S G G T S P H D G L D P F M Protein_Len: 30 Strand: - Start: 6 End: 92 ..... * P Y R A R I R A Y Y R L P G C R D G K G V T A C G S H R M ................................................................................................................................................................................................. Protein_Len: 41 Strand: - Start: 2 End: 124 . E P T G L E F E P T I A F L G A G I A R G L L Q A A Q I A F P A Q H P I K E K R M ................................................................................................................................................................. Protein_Len: 34 Strand: - Start: 1 End: 102 S L P V S S S N Q R L L S S A R V S R G E W C N R L R F P S H R R M ....................................................................................................................................................................................... annnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngcgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ annnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngcgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_2001:SMT_2002|SMT_2001:SMT_2002:membrane protease protein family:hypothetical protein predicted by Glimmer/Critica:-><-:1923950..1924234 285 annnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngcgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Intra-Species Hit: Count: 36 Min: 162 Max: 285 Len: 124 Subject: smit_SMT_2001_SMT_2002|membrane protease protein family:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1923950,1924234]|285 HSP 1 e-value: 4.0E-64 bit: 246.0 Len: 124 Query Start:162 Query End:285 Subject Strand: POSITIVE Subject Start: 162 Subject End: 285 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_2029_SMT_2030|acetyltransferase, GNAT family:chromosomal replication initiator protein DnaA|POSITIVE:NEGATIVE|[1944345,1944879]|535 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: POSITIVE Subject Start: 355 Subject End: 478 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_2016_SMT_2017|tyrosine recombinase:exonuclease RexA|NEGATIVE:NEGATIVE|[1933516,1935951]|2436 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: NEGATIVE Subject Start: 67 Subject End: 190 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_2000_SMT_2001|hypothetical protein:membrane protease protein family|NEGATIVE:POSITIVE|[1923199,1923526]|328 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: POSITIVE Subject Start: 148 Subject End: 271 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1985_SMT_1986|hypothetical protein predicted by Glimmer/Critica:mercuric reductase|NEGATIVE:POSITIVE|[1914662,1914982]|321 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: NEGATIVE Subject Start: 146 Subject End: 269 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1978_SMT_1979|hypothetical protein:possible cell surface protein|NEGATIVE:NEGATIVE|[1909657,1910371]|715 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: POSITIVE Subject Start: 498 Subject End: 621 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1967_SMT_1968|dicarboxylate/amino acid:cation (Na+ or H+) symporter (DAACS) family protein:transcription-repair coupling factor|NEGATIVE:NEGATIVE|[1899774,1900040]|267 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: NEGATIVE Subject Start: 3 Subject End: 126 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1966_SMT_1967|DNA polymerase III alpha subunit:dicarboxylate/amino acid:cation (Na+ or H+) symporter (DAACS) family protein|POSITIVE:NEGATIVE|[1898389,1899587]|1199 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 124 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag HSP 2 e-value: 5.0E-20 bit: 99.6 Len: 54 Query Start:162 Query End:215 Subject Strand: NEGATIVE Subject Start: 227 Subject End: 280 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcaga...................................................................... .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcaga...................................................................... Subject: smit_SMT_1953_SMT_1954|hypothetical protein:6-phospho-beta- glucosidase|POSITIVE:POSITIVE|[1888168,1888687]|520 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: POSITIVE Subject Start: 351 Subject End: 474 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1946_SMT_1947|sucrose-6-phosphate hydrolase:lysine decarboxylase|POSITIVE:NEGATIVE|[1881266,1882402]|1137 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: NEGATIVE Subject Start: 477 Subject End: 600 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1944_SMT_1945|hypothetical protein predicted by Glimmer/Critica:putative dihydrolipoamide S-acetyltransferase|NEGATIVE:POSITIVE|[1879585, 1880100]|516 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: NEGATIVE Subject Start: 38 Subject End: 161 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1926_SMT_1927|mevalonate kinase:cell division protein FtsH|POSITIVE:NEGATIVE|[1868123,1868497]|375 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: POSITIVE Subject Start: 252 Subject End: 375 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag HSP 2 e-value: 3.0E-55 bit: 216.0 Len: 113 Query Start:162 Query End:274 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 113 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga........... .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga........... Subject: smit_SMT_1895_SMT_1896|hypothetical protein predicted by Glimmer/Critica:lysine decarboxylase|POSITIVE:NEGATIVE|[1847463,1848162]|700 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: NEGATIVE Subject Start: 62 Subject End: 185 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1885_SMT_1886|hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1840148,1841123]|976 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: POSITIVE Subject Start: 651 Subject End: 774 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1884_SMT_1885|hypothetical protein:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1839299,1839919]|621 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: POSITIVE Subject Start: 498 Subject End: 621 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1873_SMT_1874|K02006 cobalt transport system ATP-binding protein:hypothetical protein|POSITIVE:NEGATIVE|[1832412,1832712]|301 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 124 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1857_SMT_1858|hypothetical protein:K03497 chromosome partitioning protein, ParB family|POSITIVE:NEGATIVE|[1821206,1821776]|571 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: NEGATIVE Subject Start: 77 Subject End: 200 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1849_SMT_1850|hypothetical protein:K01421 putative membrane protein|POSITIVE:POSITIVE|[1813865,1815201]|1337 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: POSITIVE Subject Start: 870 Subject End: 993 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1690_SMT_1691|K06148 ATP-binding cassette, subfamily C, bacterial:putative deoxyribonuclease|NEGATIVE:POSITIVE|[1674870, 1675117]|248 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: NEGATIVE Subject Start: 3 Subject End: 126 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1685_SMT_1686|phenylalanyl-tRNA synthetase alpha chain:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1668936,1670314]|1379 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: POSITIVE Subject Start: 1178 Subject End: 1301 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_779_SMT_780|ribonuclease BN, putative:peptidase family U32|NEGATIVE:NEGATIVE|[688621,688841]|221 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: NEGATIVE Subject Start: 3 Subject End: 126 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_478_SMT_479|hypothetical protein predicted by Glimmer/Critica:K03308 neurotransmitter:Na+ symporter, NSS family|POSITIVE:NEGATIVE|[415318,415693]|376 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: POSITIVE Subject Start: 200 Subject End: 323 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_387_SMT_388|hypothetical protein predicted by Glimmer/Critica:branched-chain amino acid transport system II carrier protein|POSITIVE:POSITIVE|[321179,321311]|133 HSP 1 e-value: 9.0E-62 bit: 238.0 Len: 124 Query Start:162 Query End:285 Subject Strand: POSITIVE Subject Start: 8 Subject End: 131 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1845_SMT_1846|K01191 alpha-mannosidase:acyl-ACP thioesterase, putative|POSITIVE:POSITIVE|[1810496,1811549]|1054 HSP 1 e-value: 2.0E-59 bit: 230.0 Len: 124 Query Start:162 Query End:285 Subject Strand: POSITIVE Subject Start: 584 Subject End: 707 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaarscgcctmtccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1558_SMT_1559|hypothetical protein:6- phosphofructokinase|NEGATIVE:NEGATIVE|[1544149, 1544703]|555 HSP 1 e-value: 3.0E-58 bit: 226.0 Len: 124 Query Start:162 Query End:285 Subject Strand: NEGATIVE Subject Start: 193 Subject End: 315 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag .................................................................................................................................................................cgcccaatacgcaacc-gcctctccccgcgcgttggccgattcattaatgcrgagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1955_SMT_1956|carbamoyl-phosphate synthase small chain:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:NEGATIVE|[1889499,1889626]|128 HSP 1 e-value: 1.0E-57 bit: 224.0 Len: 121 Query Start:165 Query End:285 Subject Strand: NEGATIVE Subject Start: 3 Subject End: 123 ....................................................................................................................................................................ccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag ....................................................................................................................................................................ccaatacgcaaaccgcttctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_2024_SMT_2025|hypothetical protein:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1940751,1941084]|334 HSP 1 e-value: 3.0E-55 bit: 216.0 Len: 113 Query Start:162 Query End:274 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 113 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga........... .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga........... Subject: smit_SMT_1981_SMT_1982|K03696 ATP-dependent Clp protease ATP-binding subunit ClpC:conserved hypothetical protein|POSITIVE:POSITIVE|[1912929,1913175]|247 HSP 1 e-value: 3.0E-55 bit: 216.0 Len: 113 Query Start:162 Query End:274 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 113 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga........... .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga........... Subject: smit_SMT_1942_SMT_1943|zinc metalloprotease ZmpB, truncated:alpha/beta hydrolase fold|POSITIVE:NEGATIVE|[1878445,1878659]|215 HSP 1 e-value: 3.0E-55 bit: 216.0 Len: 113 Query Start:162 Query End:274 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 113 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga........... .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga........... Subject: smit_SMT_1887_SMT_1888|LicC protein:K02026 multiple sugar transport system permease protein|POSITIVE:POSITIVE|[1841801,1842227]|427 HSP 1 e-value: 3.0E-55 bit: 216.0 Len: 113 Query Start:162 Query End:274 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 113 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga........... .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga........... Subject: smit_SMT_1851_SMT_1852|ABC transporter, permease protein, putative:1,4-alpha-glucan branching enzyme|NEGATIVE:NEGATIVE|[1815812,1816556]|745 HSP 1 e-value: 3.0E-52 bit: 206.0 Len: 108 Query Start:162 Query End:269 Subject Strand: POSITIVE Subject Start: 638 Subject End: 745 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac................ .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac................ Subject: smit_SMT_325_SMT_326|K02029 polar amino acid transport system permease protein:K02029 polar amino acid transport system permease protein|NEGATIVE:NEGATIVE|[263114,263306]|193 HSP 1 e-value: 3.0E-52 bit: 206.0 Len: 108 Query Start:162 Query End:269 Subject Strand: POSITIVE Subject Start: 86 Subject End: 193 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac................ .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac................ Subject: smit_SMT_2044_SMT_2045|transcriptional activator TipA, putative:K+ transport system, NAD-binding component|NEGATIVE:POSITIVE|[1955976,1956278]|303 HSP 1 e-value: 5.0E-51 bit: 202.0 Len: 122 Query Start:164 Query End:285 Subject Strand: NEGATIVE Subject Start: 82 Subject End: 203 ...................................................................................................................................................................cccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag ...................................................................................................................................................................cccaatacgcaaaccgcctctccccgcgcgtgggcgatttcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_1971_SMT_1972|hypothetical protein predicted by Glimmer/Critica:uncharacterized conserved phage related protein|POSITIVE:POSITIVE|[1903587,1903801]|215 HSP 1 e-value: 7.0E-50 bit: 198.0 Len: 108 Query Start:162 Query End:269 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 108 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac................ .................................................................................................................................................................cgcccaatacgcaaaccgcttctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac................ Subject: smit_SMT_303_SMT_304|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[245819,246719]|901 HSP 1 e-value: 2.0E-47 bit: 190.0 Len: 100 Query Start:162 Query End:261 Subject Strand: POSITIVE Subject Start: 802 Subject End: 901 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctagg........................ .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctagg........................ HSP 2 e-value: 7.0E-47 bit: 188.0 Len: 99 Query Start:187 Query End:285 Subject Strand: NEGATIVE Subject Start: 121 Subject End: 219 ..........................................................................................................................................................................................ccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag ..........................................................................................................................................................................................ccgcgcgttggccgattcataaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag Subject: smit_SMT_2005_SMT_2006|pullulanase, type I:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1926380,1926611]|232 HSP 1 e-value: 8.0E-16 bit: 85.7 Len: 43 Query Start:243 Query End:285 Subject Strand: POSITIVE Subject Start: 109 Subject End: 151 ..................................................................................................................................................................................................................................................aggatccccctgtgctaggatggtcacctagatcaggaaacag ..................................................................................................................................................................................................................................................aggatccccctgtgctaggatggtcacctagatcaggaaacag HSP 2 e-value: 7.0E-7 bit: 56.0 Len: 32 Query Start:162 Query End:193 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 32 .................................................................................................................................................................cgcccaatacgcaacccgcctctccccgcgcg............................................................................................ .................................................................................................................................................................cgcccaatacgcaaaccgcctctccccgcgcg............................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGCCCAAUACGCAACCCGCCUCUCCCCGCGCGUUGGCCGAUUCAUUAAUGCAGAGCUUGCAUGCCUGCAGGUCGACUCUAGAGGAUCCCCCUGUGCUAGGAUGGUCACCUAGAUCAGGAAACAG ((.((((..(((...............)))..)))).)).........(((((.((......)))))))....((.(((((..((((..((((...))))..))))..))))))).(....).. (-36.56) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUGUUUCCUGAUCUAGGUGACCAUCCUAGCACAGGGGGAUCCUCUAGAGUCGACCUGCAGGCAUGCAAGCUCUGCAUUAAUGAAUCGGCCAACGCGCGGGGAGAGGCGGGUUGCGUAUUGGGCG ...(..((((..(((((.......)))))..))))..).(((....((.(((...(((((((......)).)))))....))).)).((((((.(((........))).)))).))...))).. (-37.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1095 70 21 213 164 smit:SMT_1986|5end_mercuric_reductase_1914843..1915073_POSITIVE 1048 50 1 226 177 smit:SMT_325|5end_K02029_polar_amino_acid_transport_system_permease_protein_263023..263253_NEGATIVE 1039 100 51 190 141 smit:SMT_959|5end_1-acyl-sn-glycerol-3-phosphate_acyltransferase_905283..905513_POSITIVE 873 84 41 231 188 smit:SMT_1914|5end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1859486..1859716_POSITIVE 724 42 3 230 192 smit:SMT_2045|5end_K+_transport_system,_NAD-binding_component_1956139..1956369_POSITIVE 654 113 82 231 200 smit:SMT_2005|5end_pullulanase,_type_I_1926289..1926519_NEGATIVE 637 33 1 231 199 smit:SMT_1972|5end_uncharacterized_conserved_phage_related_protein_1903662..1903892_POSITIVE 396 70 23 137 76 smit:SMT_941|5end_hypothetical_protein_893558..893788_NEGATIVE 357 110 61 195 130 smit:SMT_849|5end_hypothetical_protein_796127..796357_NEGATIVE 353 75 31 180 128 smit:SMT_110|5end_alpha-L-fucosidase_57445..57675_POSITIVE 350 69 28 71 30 smit:SMT_1634|5end_2,3,4,5-tetrahydropyridine-2-carboxylate_N-succinyltransferase,_putative_1621074..1621304_POSITIVE 345 118 71 153 103 smit:SMT_1291|5end_hypothetical_protein_1253875..1254105_POSITIVE 338 99 54 61 2 smit:SMT_1120|5end_hypothetical_protein_1057063..1057293_NEGATIVE 333 105 61 65 17 smit:SMT_1602|5end_hypothetical_protein_1592385..1592615_POSITIVE 332 70 21 159 96 smit:SMT_1647|5end_3-isopropylmalate_dehydratase_small_subunit_1633403..1633633_POSITIVE 325 44 1 185 129 smit:SMT_1201|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1163920..1164150_NEGATIVE 324 60 18 231 180 smit:SMT_155|5end_hypothetical_protein_102704..102934_NEGATIVE 323 46 1 214 160 smit:SMT_1626|5end_undecaprenyl-diphosphatase_1616028..1616258_NEGATIVE 323 69 23 229 175 smit:SMT_187|5end_30S_ribosomal_protein_S5_122997..123227_POSITIVE 322 88 42 159 88 smit:SMT_1915|5end_pyridoxine_biosynthesis_protein_1860329..1860559_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1117 100 51 77 28 smit:SMT_2024|3end_hypothetical_protein_1940690..1940840_POSITIVE 1117 100 51 77 28 smit:SMT_1981|3end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1912868..1913018_POSITIVE 1117 100 51 82 33 smit:SMT_1971|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1903526..1903676_POSITIVE 1117 100 51 66 17 smit:SMT_1966|3end_DNA_polymerase_III_alpha_subunit_1898328..1898478_POSITIVE 1117 100 51 77 28 smit:SMT_1942|3end_zinc_metalloprotease_ZmpB,_truncated_1878384..1878534_POSITIVE 1117 100 51 66 17 smit:SMT_1927|3end_cell_division_protein_FtsH_1868408..1868558_NEGATIVE 1117 100 51 77 28 smit:SMT_1926|3end_mevalonate_kinase_1868062..1868212_POSITIVE 1117 100 51 77 28 smit:SMT_1887|3end_LicC_protein_1841740..1841890_POSITIVE 1117 100 51 66 17 smit:SMT_1885|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1839830..1839980_NEGATIVE 1117 100 51 66 17 smit:SMT_1873|3end_K02006_cobalt_transport_system_ATP-binding_protein_1832351..1832501_POSITIVE 1117 100 51 82 33 smit:SMT_1852|3end_1,4-alpha-glucan_branching_enzyme_1816467..1816617_NEGATIVE 1117 100 51 82 33 smit:SMT_326|3end_K02029_polar_amino_acid_transport_system_permease_protein_263217..263367_NEGATIVE 1039 100 51 66 17 smit:SMT_958|3end_hypothetical_protein_predicted_by_Glimmer/Critica_905239..905389_POSITIVE 657 120 88 33 1 smit:SMT_479|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_415604..415754_NEGATIVE 529 120 92 29 1 smit:SMT_2030|3end_chromosomal_replication_initiator_protein_DnaA_1944790..1944940_NEGATIVE 435 120 96 25 1 smit:SMT_1895|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1847402..1847552_POSITIVE 332 70 21 149 86 smit:SMT_1646|3end_3-isopropylmalate_dehydratase_large_subunit_1633473..1633623_POSITIVE 322 88 42 74 3 smit:SMT_1914|3end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1860324..1860474_POSITIVE 321 37 2 66 29 smit:SMT_1941|3end_hypothetical_protein_1877663..1877813_POSITIVE 319 59 16 39 5 smit:SMT_1612|3end_K06024_segregation_and_condensation_protein_B_1600701..1600851_POSITIVE