Origin IGS: ggcactggaaatcaccgtcgtgatttcaatatgattcagcaaatattctcgcaytttggatgactcacttgggacaaatcaagctgatctaccaagagtcgatgacttccagcacacttgnnnnnttaattaattaannnnnaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagctatgaccccagtgtgctggaaagcatataatcttcaagttggtaagaaaacatttg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 caaatgttttcttaccaacttgaagattatatgctttccagcacactggggtcatagctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttnnnnnttaattaattaannnnncaagtgtgctggaagtcatcgactcttggtagatcagcttgatttgtcccaagtgagtcatccaaartgcgagaatatttgctgaatcatattgaaatcacgacggtgatttccagtgcc Mask Tandem Repeat Region ================================================ ggcactggaaatcaccgtcgtgatttcaatatgattcagcaaatattctcgcaytttggatgactcacttgggacaaatcaagctgatctaccaagagtcgatgacttccagcacacttgnnnnnttaattaattaannnnnaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagctatgaccccagtgtgctggaaagcatataatcttcaagttggtaagaaaacatttg Find is-nt database================================================ Query_seq: SMT_2000:SMT_2001|SMT_2000:SMT_2001:hypothetical protein:membrane protease protein family:<-->:1923199..1923526 328 ggcactggaaatcaccgtcgtgatttcaatatgattcagcaaatattctcgcaytttggatgactcacttgggacaaatcaagctgatctaccaagagtcgatgacttccagcacacttgnnnnnttaattaattaannnnnaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagctatgaccccagtgtgctggaaagcatataatcttcaagttggtaagaaaacatttg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_2000:SMT_2001|SMT_2000:SMT_2001:hypothetical protein:membrane protease protein family:<-->:1923199..1923526 328 ggcactggaaatcaccgtcgtgatttcaatatgattcagcaaatattctcgcaytttggatgactcacttgggacaaatcaagctgatctaccaagagtcgatgacttccagcacacttgnnnnnttaattaattaannnnnaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagctatgaccccagtgtgctggaaagcatataatcttcaagttggtaagaaaacatttg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_2000:SMT_2001|SMT_2000:SMT_2001:hypothetical protein:membrane protease protein family:<-->:1923199..1923526 328 ggcactggaaatcaccgtcgtgatttcaatatgattcagcaaatattctcgcaytttggatgactcacttgggacaaatcaagctgatctaccaagagtcgatgacttccagcacacttgnnnnnttaattaattaannnnnaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagctatgaccccagtgtgctggaaagcatataatcttcaagttggtaagaaaacatttg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 1 Max: 134 Len: 134 Subject: UniRef90_Q8CZC4 Cluster: Hypothetical protein spr0073; n=1; Streptococcus pneumoniae R6|Rep: Hypothetical protein spr0073 - Streptococcus pneumoniae (strain ATCC BAA-255 / R6) HSP 1 e-value: 2.0E-8 bit: 46.2 Len: 60 Query Start:1 Query End:60 Subject Strand: null Subject Start: 58 Subject End: 77 G T G N H R R D F N M I Q Q I F S H F G ............................................................................................................................................................................................................................................................................ G T G N H R R E F N M I Q Q I F S H F G ............................................................................................................................................................................................................................................................................ HSP 2 e-value: 2.0E-8 bit: 33.9 Len: 75 Query Start:60 Query End:134 Subject Strand: null Subject Start: 78 Subject End: 102 ........................................................... M T H L G Q I K L I Y Q E S M T S S T L X X L I N .................................................................................................................................................................................................. ........................................................... M T H L G Q I K L V Y Q E S I D L E L L V N A L N .................................................................................................................................................................................................. nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaannnnnaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagcatataatcttcaagttggtaagaaaacatttg Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 45 End: 134 ............................................ M L A X W M T H L G Q I K L I Y Q E S M T S S T L X X L I N .................................................................................................................................................................................................. Protein_Len: 40 Strand: + Start: 180 End: 299 ................................................................................................................................................................................... M A D S L M Q S L H A C R S T L E D P P V L G W S P R S G N S Y D P S V L E S I ............................. Protein_Len: 45 Strand: - Start: 137 End: 271 ........................................................................................................................................ X X L A G L V C V A E G R A N A S E N I C L K C A Q L D V R S S G G T S P H D G L D P F M ......................................................... Protein_Len: 41 Strand: - Start: 133 End: 255 .................................................................................................................................... N X X X L A W Y A F R R E G R T P R N M L A S S A H R C T S E L P D G Q A L I T M ......................................................................... nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaannnnnaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagcatataatcttcaagttggtaagaaaacatttg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaannnnnaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagcatataatcttcaagttggtaagaaaacatttg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_2000:SMT_2001|SMT_2000:SMT_2001:hypothetical protein:membrane protease protein family:<-->:1923199..1923526 328 nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaannnnnaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagcatataatcttcaagttggtaagaaaacatttg Intra-Species Hit: Count: 1 Min: 143 Max: 328 Len: 186 Subject: smit_SMT_2000_SMT_2001|hypothetical protein:membrane protease protein family|NEGATIVE:POSITIVE|[1923199,1923526]|328 HSP 1 e-value: 3.0E-34 bit: 147.0 Len: 74 Query Start:143 Query End:216 Subject Strand: POSITIVE Subject Start: 143 Subject End: 216 ..............................................................................................................................................aagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgca................................................................................................................ ..............................................................................................................................................aagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgca................................................................................................................ HSP 2 e-value: 5.0E-11 bit: 69.9 Len: 35 Query Start:294 Query End:328 Subject Strand: POSITIVE Subject Start: 294 Subject End: 328 .....................................................................................................................................................................................................................................................................................................agcatataatcttcaagttggtaagaaaacatttg .....................................................................................................................................................................................................................................................................................................agcatataatcttcaagttggtaagaaaacatttg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGAGCGCCCAAUACGCAAACCGCCUCUCCCCGCGCGUUGGCCGAUUCAUUAAUGCAGAGCUUGCAUGCCUGCAGGUCGACUCUAGAGGAUCCCCCUGUGCUAGGAUGGUCACCUAGAUCAGGAAACAGCUAUGACCCCAGUGUGCUGGAAAGCAUAUAAUCUUCAAGUUGGUAAGAAAACAUUUG ..(((((.((((..(((...............)))..)))).)).))).....(((((.((......)))))))....((.(((((..((((..((((...))))..))))..)))))))......(((((.(((.....(((((((....))))))).....))))))))............... (-53.06) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAAAUGUUUUCUUACCAACUUGAAGAUUAUAUGCUUUCCAGCACACUGGGGUCAUAGCUGUUUCCUGAUCUAGGUGACCAUCCUAGCACAGGGGGAUCCUCUAGAGUCGACCUGCAGGCAUGCAAGCUCUGCAUUAAUGAAUCGGCCAACGCGCGGGGAGAGGCGGUUUGCGUAUUGGGCGCUCUU .....((((((..........))))))..((((..((((((....)))))).))))....(..((((..(((((.......)))))..))))..).......((((......(((((((......)).))))).........((.((((..((((((..(...)..))))))..)))).)))))). (-49.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1137 50 1 188 139 smit:SMT_1986|5end_mercuric_reductase_1914843..1915073_POSITIVE 1137 50 1 221 172 smit:SMT_325|5end_K02029_polar_amino_acid_transport_system_permease_protein_263023..263253_NEGATIVE 1019 110 61 195 146 smit:SMT_959|5end_1-acyl-sn-glycerol-3-phosphate_acyltransferase_905283..905513_POSITIVE 979 89 42 231 184 smit:SMT_1914|5end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1859486..1859716_POSITIVE 795 38 1 231 194 smit:SMT_1972|5end_uncharacterized_conserved_phage_related_protein_1903662..1903892_POSITIVE 775 47 8 230 192 smit:SMT_2045|5end_K+_transport_system,_NAD-binding_component_1956139..1956369_POSITIVE 654 118 87 231 200 smit:SMT_2005|5end_pullulanase,_type_I_1926289..1926519_NEGATIVE 444 177 132 200 157 smit:SMT_1671|5end_hypothetical_protein_1659757..1659987_NEGATIVE 432 130 103 231 204 smit:SMT_712|5end_alcohol_dehydrogenase,_zinc-containing_620381..620611_NEGATIVE 431 23 1 231 209 smit:SMT_1691|5end_putative_deoxyribonuclease_1674978..1675208_POSITIVE 408 80 33 180 131 smit:SMT_110|5end_alpha-L-fucosidase_57445..57675_POSITIVE 392 154 114 195 153 smit:SMT_543|5end_topoisomerase_IV_subunit_B_458372..458602_POSITIVE 385 80 31 120 57 smit:SMT_2052|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1961591..1961821_NEGATIVE 375 150 101 211 163 smit:SMT_389|5end_PTS_system,_galactitol-specific_IIA_component,_putative_321779..322009_POSITIVE 371 79 33 142 88 smit:SMT_941|5end_hypothetical_protein_893558..893788_NEGATIVE 367 49 1 212 156 smit:SMT_1626|5end_undecaprenyl-diphosphatase_1616028..1616258_NEGATIVE 350 74 33 71 30 smit:SMT_1634|5end_2,3,4,5-tetrahydropyridine-2-carboxylate_N-succinyltransferase,_putative_1621074..1621304_POSITIVE 347 88 41 54 3 smit:SMT_1527|5end_two-component_sensor_kinase_1511183..1511413_NEGATIVE 342 154 114 81 33 smit:SMT_1942|5end_zinc_metalloprotease_ZmpB,_truncated_1877882..1878112_POSITIVE 341 109 66 182 130 smit:SMT_849|5end_hypothetical_protein_796127..796357_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1097 110 61 82 33 smit:SMT_2024|3end_hypothetical_protein_1940690..1940840_POSITIVE 1097 110 61 71 22 smit:SMT_2002|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1924145..1924295_NEGATIVE 1097 110 61 82 33 smit:SMT_1981|3end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1912868..1913018_POSITIVE 1097 110 61 87 38 smit:SMT_1971|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1903526..1903676_POSITIVE 1097 110 61 71 22 smit:SMT_1966|3end_DNA_polymerase_III_alpha_subunit_1898328..1898478_POSITIVE 1097 110 61 82 33 smit:SMT_1942|3end_zinc_metalloprotease_ZmpB,_truncated_1878384..1878534_POSITIVE 1097 110 61 71 22 smit:SMT_1927|3end_cell_division_protein_FtsH_1868408..1868558_NEGATIVE 1097 110 61 82 33 smit:SMT_1926|3end_mevalonate_kinase_1868062..1868212_POSITIVE 1097 110 61 82 33 smit:SMT_1887|3end_LicC_protein_1841740..1841890_POSITIVE 1097 110 61 71 22 smit:SMT_1885|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1839830..1839980_NEGATIVE 1097 110 61 71 22 smit:SMT_1873|3end_K02006_cobalt_transport_system_ATP-binding_protein_1832351..1832501_POSITIVE 1097 110 61 87 38 smit:SMT_1852|3end_1,4-alpha-glucan_branching_enzyme_1816467..1816617_NEGATIVE 1097 110 61 87 38 smit:SMT_326|3end_K02029_polar_amino_acid_transport_system_permease_protein_263217..263367_NEGATIVE 1022 150 101 54 5 smit:SMT_2030|3end_chromosomal_replication_initiator_protein_DnaA_1944790..1944940_NEGATIVE 1022 150 101 50 1 smit:SMT_1895|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1847402..1847552_POSITIVE 1022 150 101 58 9 smit:SMT_479|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_415604..415754_NEGATIVE 1019 110 61 71 22 smit:SMT_958|3end_hypothetical_protein_predicted_by_Glimmer/Critica_905239..905389_POSITIVE 904 150 103 127 80 smit:SMT_713|3end_heat_shock_protein_HtpX_620505..620655_NEGATIVE 764 154 116 39 1 smit:SMT_1857|3end_hypothetical_protein_1821145..1821295_POSITIVE 517 153 127 27 1 smit:SMT_1928|3end_O-methyltransferase_family_protein_1869158..1869308_NEGATIVE