Origin IGS: atatgaaccaagtttgcggtagatgattgtttcttcgctatctttatcagtagctttaacctctgtacgctctgtarattagctgctgagctacatatcctggtttgtttggagttgttacnnnnnttaattaattaannnnntgtgctggaaagtaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttactttccagcacannnnnttaattaattaannnnngtaacaactccaaacaaaccaggatatgtagctcagcagctaatytacagagcgtacagaggttaaagctactgataaagatagcgaagaaacaatcatctaccgcaaacttggttcatat Mask Tandem Repeat Region ================================================ atatgaaccaagtttgcggtagatgattgtttcttcgctatctttatcagtagctttaacctctgtacgctctgtarattagctgctgagctacatatcctggtttgtttggagttgttacnnnnnttaattaattaannnnntgtgctggaaagtaa Find is-nt database================================================ Query_seq: SMT_1979:SMT_1980|SMT_1979:SMT_1980:possible cell surface protein:glycosyltransferase:<-->:1911143..1911300 158 atatgaaccaagtttgcggtagatgattgtttcttcgctatctttatcagtagctttaacctctgtacgctctgtarattagctgctgagctacatatcctggtttgtttggagttgttacnnnnnttaattaattaannnnntgtgctggaaagtaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1979:SMT_1980|SMT_1979:SMT_1980:possible cell surface protein:glycosyltransferase:<-->:1911143..1911300 158 atatgaaccaagtttgcggtagatgattgtttcttcgctatctttatcagtagctttaacctctgtacgctctgtarattagctgctgagctacatatcctggtttgtttggagttgttacnnnnnttaattaattaannnnntgtgctggaaagtaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1979:SMT_1980|SMT_1979:SMT_1980:possible cell surface protein:glycosyltransferase:<-->:1911143..1911300 158 atatgaaccaagtttgcggtagatgattgtttcttcgctatctttatcagtagctttaacctctgtacgctctgtarattagctgctgagctacatatcctggtttgtttggagttgttacnnnnnttaattaattaannnnntgtgctggaaagtaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atatgaaccaagtttgcggtagatgattgtttcttcgctatctttatcagtagctttaacctctgtacgctctgtarattagctgctgagctacatatcctggtttgtttggagttgttacnnnnnttaattaattaannnnntgtgctggaaagtaa Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 40 End: 135 ....................................... M F I S S F N L C T L C X L A A E L H I L V C L E L L X X L I N ....................... Protein_Len: 43 Strand: - Start: 3 End: 131 .. H V L N A T S S Q K K A I K I L L K L R Q V S Q X N A A S S C I R T Q K S N N X X X M ........................... atatgaaccaagtttgcggtagatgattgtttcttcgctatctttatcagtagctttaacctctgtacgctctgtarattagctgctgagctacatatcctggtttgtttggagttgttacnnnnnttaattaattaannnnntgtgctggaaagtaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atatgaaccaagtttgcggtagatgattgtttcttcgctatctttatcagtagctttaacctctgtacgctctgtarattagctgctgagctacatatcctggtttgtttggagttgttacnnnnnttaattaattaannnnntgtgctggaaagtaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1979:SMT_1980|SMT_1979:SMT_1980:possible cell surface protein:glycosyltransferase:<-->:1911143..1911300 158 atatgaaccaagtttgcggtagatgattgtttcttcgctatctttatcagtagctttaacctctgtacgctctgtarattagctgctgagctacatatcctggtttgtttggagttgttacnnnnnttaattaattaannnnntgtgctggaaagtaa Intra-Species Hit: Count: 2 Min: 1 Max: 158 Len: 158 Subject: smit_SMT_1979_SMT_1980|possible cell surface protein:glycosyltransferase|NEGATIVE:POSITIVE|[1911143, 1911300]|158 HSP 1 e-value: 5.0E-66 bit: 251.0 Len: 158 Query Start:1 Query End:158 Subject Strand: POSITIVE Subject Start: 1 Subject End: 158 atatgaaccaagtttgcggtagatgattgtttcttcgctatctttatcagtagctttaacctctgtacgctctgtarattagctgctgagctacatatcctggtttgtttggagttgttacnnnnnttaattaattaannnnntgtgctggaaagtaa atatgaaccaagtttgcggtagatgattgtttcttcgctatctttatcagtagctttaacctctgtacgctctgtarattagctgctgagctacatatcctggtttgtttggagttgttacnnnnnttaattaattaannnnntgtgctggaaagtaa Subject: smit_SMT_1956_SMT_1957|hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1889801,1890784]|984 HSP 1 e-value: 2.0E-53 bit: 209.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 123 atatgaaccaagtttgcggtagatgattgtttcttcgctatctttatcagtagctttaacctctgtacgctctgtara-ttagctgctg-agctacatatcctggtttgtttggagttgttac..................................... atatgaaccaagtttgcggtagatgattgtttcttcgctatctttatcagtagctttaacctctgtacgctctgtagatttagctgctgaagctacatatcctggtttgtttggagttgttac..................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAUGAACCAAGUUUGCGGUAGAUGAUUGUUUCUUCGCUAUCUUUAUCAGUAGCUUUAACCUCUGUACGCUCUGUARAUUAGCUGCUGAGCUACAUAUCCUGGUUUGUUUGGAGUUGUUACNNNNNUUAAUUAAUUAANNNNNUGUGCUGGAAAGUAA ..(..(((((.....((((.(((........)))))))........(((((((((.........(....)..........)))))))))...........)))))..).....................................((((....)))). (-27.91) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUACUUUCCAGCACANNNNNUUAAUUAAUUAANNNNNGUAACAACUCCAAACAAACCAGGAUAUGUAGCUCAGCAGCUAAUYUACAGAGCGUACAGAGGUUAAAGCUACUGAUAAAGAUAGCGAAGAAACAAUCAUCUACCGCAAACUUGGUUCAUAU .....................................................(((((((.....(((((....))))).........(((...(((.(((...((((((.....)).)))).....))).....)))..)))...)))))))..... (-22.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 869 100 51 191 142 smit:SMT_1979|5end_possible_cell_surface_protein_1911052..1911282_NEGATIVE 860 70 21 161 112 smit:SMT_1956|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1889710..1889940_NEGATIVE 376 103 67 172 137 smit:SMT_1647|5end_3-isopropylmalate_dehydratase_small_subunit_1633403..1633633_POSITIVE 313 69 25 128 82 smit:SMT_168|5end_anaerobic_ribonucleoside-triphosphate_reductase_activating_protein_113670..113900_POSITIVE 303 60 12 199 140 smit:SMT_1046|5end_chaperonin_GroEL_987904..988134_NEGATIVE 303 60 12 199 140 smit:SMT_1044|5end_chaperonin_GroEL_(HSP60_family)_987435..987665_NEGATIVE 298 88 48 63 25 smit:SMT_1785|5end_phenylalanyl-tRNA_synthetase_beta_chain_1763277..1763507_NEGATIVE 288 76 33 74 18 smit:SMT_2153|5end_putative_cell_division_protein_2026905..2027135_POSITIVE 288 76 33 74 18 smit:SMT_2139|5end_cell_division_protein_FtsZ_2017083..2017313_POSITIVE 288 76 33 74 18 smit:SMT_2137|5end_cell_division_protein_FtsZ_2015797..2016027_POSITIVE 288 76 33 74 18 smit:SMT_2129|5end_cell_division_protein_FtsZ_2010090..2010320_POSITIVE 288 76 33 74 18 smit:SMT_1548|5end_cell_division_protein_FtsZ_1532384..1532614_POSITIVE 286 87 41 56 6 smit:SMT_992|5end_hypothetical_protein_934882..935112_NEGATIVE 284 110 61 70 14 smit:SMT_2134|5end_hypothetical_protein_2014700..2014930_NEGATIVE 284 110 61 70 14 smit:SMT_873|5end_hypothetical_protein_821311..821541_NEGATIVE 281 61 21 169 122 smit:SMT_488|5end_dCMP_deaminase_422956..423186_POSITIVE 279 43 8 65 26 smit:SMT_1328|5end_hypothetical_protein_1304468..1304698_NEGATIVE 277 50 10 211 166 smit:SMT_1747|5end_anthranilate_synthase_component_I_1726279..1726509_POSITIVE 268 60 11 103 38 smit:SMT_2015|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1932655..1932885_POSITIVE 267 90 42 153 109 smit:SMT_869|5end_hypothetical_protein_817698..817928_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 313 69 25 135 89 smit:SMT_167|3end_acetyltransferase,_GNAT_family_113757..113907_POSITIVE 308 92 67 151 127 smit:SMT_1646|3end_3-isopropylmalate_dehydratase_large_subunit_1633473..1633623_POSITIVE 306 76 36 56 1 smit:SMT_2152|3end_cell_division_protein_2026967..2027117_POSITIVE 306 76 36 56 1 smit:SMT_2138|3end_cell_division_protein_FtsA_2017145..2017295_POSITIVE 306 76 36 56 1 smit:SMT_2136|3end_cell_division_protein_FtsA_2015859..2016009_POSITIVE 306 76 36 56 1 smit:SMT_2128|3end_cell_division_protein_FtsA_2010152..2010302_POSITIVE 306 76 36 56 1 smit:SMT_1547|3end_cell_division_protein_FtsA_1532446..1532596_POSITIVE 302 90 42 130 88 smit:SMT_2141|3end_hypothetical_protein_2018960..2019110_NEGATIVE 299 118 81 61 18 smit:SMT_2011|3end_lysyl-tRNA_synthetase_1929759..1929909_NEGATIVE 298 88 48 105 67 smit:SMT_1786|3end_phenylalanyl-tRNA_synthetase_beta_chain_1763235..1763385_NEGATIVE 294 69 22 123 62 smit:SMT_1191|3end_phosphoribosylamine--glycine_ligase_1148475..1148625_NEGATIVE 291 110 61 145 103 smit:SMT_365|3end_hypothetical_protein_301747..301897_POSITIVE 286 87 41 55 5 smit:SMT_993|3end_hypothetical_protein_934883..935033_NEGATIVE 281 61 21 150 103 smit:SMT_487|3end_transcriptional_regulator,_TetR_family_423017..423167_POSITIVE 279 43 8 55 16 smit:SMT_1329|3end_GTP-binding_protein_CgtA_1304478..1304628_NEGATIVE 277 50 10 85 40 smit:SMT_1746|3end_glutamyl_aminopeptidase_1726233..1726383_POSITIVE 268 60 11 95 30 smit:SMT_2014|3end_tryptophan_synthase_beta_chain_1932727..1932877_POSITIVE 267 100 61 65 14 smit:SMT_1925|3end_K03697_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpE_1867458..1867608_POSITIVE 267 100 61 73 22 smit:SMT_1557|3end_hypothetical_protein_1543650..1543800_POSITIVE 267 90 42 151 107 smit:SMT_870|3end_hypothetical_protein_817700..817850_NEGATIVE