Origin IGS: ctatagcccttacccttttgcccctgtctctttaattgtagccttcttnnnnnttaattaattaannnnncagagattatacaaaccaggaaacccaaccacttaac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gttaagtggttgggtttcctggtttgtataatctctgnnnnnttaattaattaannnnnaagaaggctacaattaaagagacaggggcaaaagggtaagggctatag Mask Tandem Repeat Region ================================================ ctatagcccttacccttttgcccctgtctctttaattgtagccttcttnnnnnttaattaattaannnnncagagattatacaaaccaggaaacccaaccacttaac Find is-nt database================================================ Query_seq: SMT_1974:SMT_1975|SMT_1974:SMT_1975:high-affinity zinc uptake system protein ZnuA precursor / hypothetical exported protein:hypothetical protein predicted by Glimmer/Critica:->->:1906171..1906277 107 ctatagcccttacccttttgcccctgtctctttaattgtagccttcttnnnnnttaattaattaannnnncagagattatacaaaccaggaaacccaaccacttaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1974:SMT_1975|SMT_1974:SMT_1975:high-affinity zinc uptake system protein ZnuA precursor / hypothetical exported protein:hypothetical protein predicted by Glimmer/Critica:->->:1906171..1906277 107 ctatagcccttacccttttgcccctgtctctttaattgtagccttcttnnnnnttaattaattaannnnncagagattatacaaaccaggaaacccaaccacttaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1974:SMT_1975|SMT_1974:SMT_1975:high-affinity zinc uptake system protein ZnuA precursor / hypothetical exported protein:hypothetical protein predicted by Glimmer/Critica:->->:1906171..1906277 107 ctatagcccttacccttttgcccctgtctctttaattgtagccttcttnnnnnttaattaattaannnnncagagattatacaaaccaggaaacccaaccacttaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctatagcccttacccttttgcccctgtctctttaattgtagccttcttnnnnnttaattaattaannnnncagagattatacaaaccaggaaacccaaccacttaac Predict ORF larger than 30AA ================================================ ctatagcccttacccttttgcccctgtctctttaattgtagccttcttnnnnnttaattaattaannnnncagagattatacaaaccaggaaacccaaccacttaac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctatagcccttacccttttgcccctgtctctttaattgtagccttcttnnnnnttaattaattaannnnncagagattatacaaaccaggaaacccaaccacttaac Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1974:SMT_1975|SMT_1974:SMT_1975:high-affinity zinc uptake system protein ZnuA precursor / hypothetical exported protein:hypothetical protein predicted by Glimmer/Critica:->->:1906171..1906277 107 ctatagcccttacccttttgcccctgtctctttaattgtagccttcttnnnnnttaattaattaannnnncagagattatacaaaccaggaaacccaaccacttaac Intra-Species Hit: Count: 1 Min: 1 Max: 107 Len: 107 Subject: smit_SMT_1974_SMT_1975|high-affinity zinc uptake system protein ZnuA precursor / hypothetical exported protein:hypothetical protein predicted by Glimmer/Critica|POSITIVE:POSITIVE|[1906171,1906277]|107 HSP 1 e-value: 1.0E-36 bit: 153.0 Len: 107 Query Start:1 Query End:107 Subject Strand: POSITIVE Subject Start: 1 Subject End: 107 ctatagcccttacccttttgcccctgtctctttaattgtagccttcttnnnnnttaattaattaannnnncagagattatacaaaccaggaaacccaaccacttaac ctatagcccttacccttttgcccctgtctctttaattgtagccttcttnnnnnttaattaattaannnnncagagattatacaaaccaggaaacccaaccacttaac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUAUAGCCCUUACCCUUUUGCCCCUGUCUCUUUAAUUGUAGCCUUCUUNNNNNUUAAUUAAUUAANNNNNCAGAGAUUAUACAAACCAGGAAACCCAACCACUUAAC .............(((((((.....(((((((((((((....................)))))))......))))))....))))..)))................. ( -8.95) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUAAGUGGUUGGGUUUCCUGGUUUGUAUAAUCUCUGNNNNNUUAAUUAAUUAANNNNNAAGAAGGCUACAAUUAAAGAGACAGGGGCAAAAGGGUAAGGGCUAUAG .....((((((......(((..(((((....(((((........................................))))).....))))).)))....)))))).. (-13.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 327 48 14 227 198 smit:SMT_730|5end_hypothetical_protein_638387..638617_NEGATIVE 298 41 1 132 88 smit:SMT_962|5end_putative_hemin_transport_system_ATP-binding_protein_fecE_908584..908814_NEGATIVE 273 42 1 127 88 smit:SMT_1396|5end_uracil-DNA_glycosylase_1372064..1372294_POSITIVE 264 47 1 225 164 smit:SMT_707|5end_hypothetical_protein_predicted_by_Glimmer/Critica_617120..617350_NEGATIVE 257 48 7 118 75 smit:SMT_1347|5end_SsrA-binding_protein_1325185..1325415_NEGATIVE 251 43 3 86 36 smit:SMT_1481|5end_endopeptidase_O_1462229..1462459_POSITIVE 246 48 4 200 147 smit:SMT_748|5end_prephenate_dehydratase_653498..653728_NEGATIVE 241 48 14 181 145 smit:SMT_1116|5end_K02244_competence_protein_ComGB_1053841..1054071_NEGATIVE 240 48 7 134 92 smit:SMT_977|5end_conserved_hypothetical_protein,_Rrf2_family_protein_925689..925919_NEGATIVE 237 44 6 213 176 smit:SMT_2002|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1924405..1924635_NEGATIVE 237 44 6 213 176 smit:SMT_1942|5end_zinc_metalloprotease_ZmpB,_truncated_1877882..1878112_POSITIVE 237 41 5 71 39 smit:SMT_382|5end_valyl-tRNA_synthetase_317706..317936_POSITIVE 236 48 6 54 23 smit:SMT_1648|5end_hypothetical_protein_1634024..1634254_POSITIVE 236 48 14 175 146 smit:SMT_1292|5end_K02050_sulfonate/nitrate/taurine_transport_system_permease_protein_1254179..1254409_POSITIVE 233 47 1 222 161 smit:SMT_708|5end_hypothetical_protein_618021..618251_NEGATIVE 231 40 6 90 53 smit:SMT_1976|5end_immunoglobulin_A1_protease_1906950..1907180_POSITIVE 230 34 4 206 165 smit:SMT_1217|5end_hypothetical_protein_1176603..1176833_NEGATIVE 228 45 4 155 113 smit:SMT_115|5end_hypothetical_protein_65521..65751_POSITIVE 227 34 6 46 9 smit:SMT_1529|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1512880..1513110_NEGATIVE 227 46 1 168 104 smit:SMT_148|5end_glycoprotein_endopeptidase_98033..98263_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 298 41 1 130 86 smit:SMT_963|3end_K02015_iron_complex_transport_system_permease_protein_908586..908736_NEGATIVE 273 42 1 65 26 smit:SMT_1395|3end_K07025_putative_hydrolase_of_the_HAD_superfamily_1372082..1372232_POSITIVE 246 43 6 151 108 smit:SMT_812|3end_acetyltransferase,_GNAT_family_721664..721814_NEGATIVE 240 48 7 58 16 smit:SMT_978|3end_hypothetical_protein_925765..925915_NEGATIVE 230 34 4 121 80 smit:SMT_1218|3end_DNA_polymerase_I_1176688..1176838_NEGATIVE 228 45 4 83 41 smit:SMT_114|3end_alpha-mannosidase_65529..65679_POSITIVE 227 34 6 150 113 smit:SMT_1529|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1512776..1512926_NEGATIVE 227 46 1 71 7 smit:SMT_147|3end_K02027_multiple_sugar_transport_system_substrate-binding_protein_98016..98166_POSITIVE 223 47 6 123 67 smit:SMT_1871|3end_modification_methylase,_HemK_family_1829803..1829953_NEGATIVE 223 42 5 48 13 smit:SMT_836|3end_cell_surface_protein_precursor_777664..777814_NEGATIVE 222 46 1 141 96 smit:SMT_1489|3end_K06147_ATP-binding_cassette,_subfamily_B,_bacterial_1470714..1470864_POSITIVE 216 39 12 50 20 smit:SMT_101|3end_cell_division_protein_34067..34217_POSITIVE 215 42 1 149 109 smit:SMT_1921|3end_D-alanine-activating_enzyme_1864101..1864251_POSITIVE 214 48 6 91 38 smit:SMT_1643|3end_2-isopropylmalate_synthase_1630772..1630922_POSITIVE 212 40 3 58 4 smit:SMT_1791|3end_hypothetical_protein_1766433..1766583_POSITIVE 211 48 1 149 81 smit:SMT_773|3end_UDP-N-acetylenolpyruvoylglucosamine_reductase_683460..683610_NEGATIVE 205 47 6 46 10 smit:SMT_605|3end_DNA-binding_protein_HU_519257..519407_POSITIVE 203 33 16 81 63 smit:SMT_1306|3end_alpha-glycerophosphate_oxidase_1271673..1271823_POSITIVE 202 43 5 114 78 smit:SMT_1353|3end_GTP-binding_protein_1330690..1330840_NEGATIVE 202 48 9 124 90 smit:SMT_1073|3end_L-asparaginase_1012251..1012401_POSITIVE