Origin IGS: gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcgnnnnnttaattaattaannnnntaccaggcgaaaggaccatacacagtgaaaccag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctggtttcactgtgtatggtcctttcgcctggtannnnnttaattaattaannnnncgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcttctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac Mask Tandem Repeat Region ================================================ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcgnnnnnttaattaattaannnnntaccaggcgaaaggaccatacacagtgaaaccag Find is-nt database================================================ Query_seq: SMT_1971:SMT_1972|SMT_1971:SMT_1972:hypothetical protein predicted by Glimmer/Critica:uncharacterized conserved phage related protein:->->:1903587..1903801 215 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcgnnnnnttaattaattaannnnntaccaggcgaaaggaccatacacagtgaaaccag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1971:SMT_1972|SMT_1971:SMT_1972:hypothetical protein predicted by Glimmer/Critica:uncharacterized conserved phage related protein:->->:1903587..1903801 215 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcgnnnnnttaattaattaannnnntaccaggcgaaaggaccatacacagtgaaaccag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1971:SMT_1972|SMT_1971:SMT_1972:hypothetical protein predicted by Glimmer/Critica:uncharacterized conserved phage related protein:->->:1903587..1903801 215 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcgnnnnnttaattaattaannnnntaccaggcgaaaggaccatacacagtgaaaccag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcgnnnnnttaattaattaannnnntaccaggcgaaaggaccatacacagtgaaaccag Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: + Start: 1 End: 129 M T I L A Q G D P L E S T C R H A S S A L M N R P T R G E K R F A Y W A L F R F L A H ...................................................................................... Protein_Len: 34 Strand: + Start: 68 End: 169 ................................................................... M G Q R A G R S G L R I G R S S A S S L T D S L R S V V R L R X X N .............................................. Protein_Len: 45 Strand: + Start: 39 End: 173 ...................................... M Q A C K L C I N E S A N A R G E A V C V L G A L P L P R S L T R C A R S F G C X X L I N .......................................... Protein_Len: 56 Strand: - Start: 2 End: 169 . S W G L V P P D E L T S R C A H L S Q M L S D A L A R P S A T Q T N P A R G S G R E S V R Q A R D N P Q X X X M .............................................. gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcgnnnnnttaattaattaannnnntaccaggcgaaaggaccatacacagtgaaaccag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcgnnnnnttaattaattaannnnntaccaggcgaaaggaccatacacagtgaaaccag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1971:SMT_1972|SMT_1971:SMT_1972:hypothetical protein predicted by Glimmer/Critica:uncharacterized conserved phage related protein:->->:1903587..1903801 215 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcgnnnnnttaattaattaannnnntaccaggcgaaaggaccatacacagtgaaaccag Intra-Species Hit: Count: 36 Min: 1 Max: 215 Len: 215 Subject: smit_SMT_1971_SMT_1972|hypothetical protein predicted by Glimmer/Critica:uncharacterized conserved phage related protein|POSITIVE:POSITIVE|[1903587,1903801]|215 HSP 1 e-value: 1.0E-100 bit: 367.0 Len: 215 Query Start:1 Query End:215 Subject Strand: POSITIVE Subject Start: 1 Subject End: 215 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcgnnnnnttaattaattaannnnntaccaggcgaaaggaccatacacagtgaaaccag gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcgnnnnnttaattaattaannnnntaccaggcgaaaggaccatacacagtgaaaccag Subject: smit_SMT_2024_SMT_2025|hypothetical protein:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1940751,1941084]|334 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 6 Subject End: 164 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_2016_SMT_2017|tyrosine recombinase:exonuclease RexA|NEGATIVE:NEGATIVE|[1933516,1935951]|2436 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 83 Subject End: 241 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1981_SMT_1982|K03696 ATP-dependent Clp protease ATP-binding subunit ClpC:conserved hypothetical protein|POSITIVE:POSITIVE|[1912929,1913175]|247 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 6 Subject End: 164 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1978_SMT_1979|hypothetical protein:possible cell surface protein|NEGATIVE:NEGATIVE|[1909657,1910371]|715 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: NEGATIVE Subject Start: 447 Subject End: 605 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1967_SMT_1968|dicarboxylate/amino acid:cation (Na+ or H+) symporter (DAACS) family protein:transcription-repair coupling factor|NEGATIVE:NEGATIVE|[1899774,1900040]|267 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 19 Subject End: 177 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1966_SMT_1967|DNA polymerase III alpha subunit:dicarboxylate/amino acid:cation (Na+ or H+) symporter (DAACS) family protein|POSITIVE:NEGATIVE|[1898389,1899587]|1199 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 17 Subject End: 175 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ HSP 2 e-value: 1.0E-50 bit: 200.0 Len: 105 Query Start:55 Query End:159 Subject Strand: POSITIVE Subject Start: 227 Subject End: 331 ......................................................tctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ ......................................................tctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1953_SMT_1954|hypothetical protein:6-phospho-beta- glucosidase|POSITIVE:POSITIVE|[1888168,1888687]|520 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: NEGATIVE Subject Start: 300 Subject End: 458 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1946_SMT_1947|sucrose-6-phosphate hydrolase:lysine decarboxylase|POSITIVE:NEGATIVE|[1881266,1882402]|1137 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 493 Subject End: 651 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1944_SMT_1945|hypothetical protein predicted by Glimmer/Critica:putative dihydrolipoamide S-acetyltransferase|NEGATIVE:POSITIVE|[1879585, 1880100]|516 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 54 Subject End: 212 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1926_SMT_1927|mevalonate kinase:cell division protein FtsH|POSITIVE:NEGATIVE|[1868123,1868497]|375 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 6 Subject End: 164 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ HSP 2 e-value: 5.0E-81 bit: 301.0 Len: 156 Query Start:1 Query End:156 Subject Strand: NEGATIVE Subject Start: 204 Subject End: 359 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggct........................................................... gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggct........................................................... Subject: smit_SMT_1895_SMT_1896|hypothetical protein predicted by Glimmer/Critica:lysine decarboxylase|POSITIVE:NEGATIVE|[1847463,1848162]|700 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 78 Subject End: 236 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1887_SMT_1888|LicC protein:K02026 multiple sugar transport system permease protein|POSITIVE:POSITIVE|[1841801,1842227]|427 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 6 Subject End: 164 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1885_SMT_1886|hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1840148,1841123]|976 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: NEGATIVE Subject Start: 600 Subject End: 758 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1884_SMT_1885|hypothetical protein:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1839299,1839919]|621 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: NEGATIVE Subject Start: 447 Subject End: 605 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1873_SMT_1874|K02006 cobalt transport system ATP-binding protein:hypothetical protein|POSITIVE:NEGATIVE|[1832412,1832712]|301 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 17 Subject End: 175 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1857_SMT_1858|hypothetical protein:K03497 chromosome partitioning protein, ParB family|POSITIVE:NEGATIVE|[1821206,1821776]|571 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 93 Subject End: 251 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1851_SMT_1852|ABC transporter, permease protein, putative:1,4-alpha-glucan branching enzyme|NEGATIVE:NEGATIVE|[1815812,1816556]|745 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: NEGATIVE Subject Start: 587 Subject End: 745 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1849_SMT_1850|hypothetical protein:K01421 putative membrane protein|POSITIVE:POSITIVE|[1813865,1815201]|1337 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: NEGATIVE Subject Start: 819 Subject End: 977 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1690_SMT_1691|K06148 ATP-binding cassette, subfamily C, bacterial:putative deoxyribonuclease|NEGATIVE:POSITIVE|[1674870, 1675117]|248 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 19 Subject End: 177 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1685_SMT_1686|phenylalanyl-tRNA synthetase alpha chain:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1668936,1670314]|1379 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: NEGATIVE Subject Start: 1127 Subject End: 1285 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_779_SMT_780|ribonuclease BN, putative:peptidase family U32|NEGATIVE:NEGATIVE|[688621,688841]|221 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 19 Subject End: 177 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_478_SMT_479|hypothetical protein predicted by Glimmer/Critica:K03308 neurotransmitter:Na+ symporter, NSS family|POSITIVE:NEGATIVE|[415318,415693]|376 HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 159 Query Start:1 Query End:159 Subject Strand: NEGATIVE Subject Start: 149 Subject End: 307 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_325_SMT_326|K02029 polar amino acid transport system permease protein:K02029 polar amino acid transport system permease protein|NEGATIVE:NEGATIVE|[263114,263306]|193 HSP 1 e-value: 3.0E-82 bit: 305.0 Len: 158 Query Start:1 Query End:158 Subject Strand: NEGATIVE Subject Start: 36 Subject End: 193 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgc......................................................... gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgc......................................................... Subject: smit_SMT_1845_SMT_1846|K01191 alpha-mannosidase:acyl-ACP thioesterase, putative|POSITIVE:POSITIVE|[1810496,1811549]|1054 HSP 1 e-value: 3.0E-79 bit: 295.0 Len: 159 Query Start:1 Query End:159 Subject Strand: NEGATIVE Subject Start: 533 Subject End: 691 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggakaggcgsyttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_303_SMT_304|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[245819,246719]|901 HSP 1 e-value: 5.0E-78 bit: 291.0 Len: 151 Query Start:9 Query End:159 Subject Strand: NEGATIVE Subject Start: 751 Subject End: 901 ........cctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ ........cctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ HSP 2 e-value: 2.0E-37 bit: 157.0 Len: 83 Query Start:1 Query End:83 Subject Strand: POSITIVE Subject Start: 137 Subject End: 219 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcgg.................................................................................................................................... gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcatttatgaatcggccaacgcgcgg.................................................................................................................................... Subject: smit_SMT_1558_SMT_1559|hypothetical protein:6- phosphofructokinase|NEGATIVE:NEGATIVE|[1544149, 1544703]|555 HSP 1 e-value: 8.0E-77 bit: 287.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 209 Subject End: 366 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctcygcattaatgaatcggccaacgcgcggggagaggcgg-ttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ Subject: smit_SMT_1942_SMT_1943|zinc metalloprotease ZmpB, truncated:alpha/beta hydrolase fold|POSITIVE:NEGATIVE|[1878445,1878659]|215 HSP 1 e-value: 1.0E-66 bit: 254.0 Len: 132 Query Start:1 Query End:132 Subject Strand: POSITIVE Subject Start: 6 Subject End: 137 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactga................................................................................... gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga................................................................................... Subject: smit_SMT_2029_SMT_2030|acetyltransferase, GNAT family:chromosomal replication initiator protein DnaA|POSITIVE:NEGATIVE|[1944345,1944879]|535 HSP 1 e-value: 4.0E-63 bit: 242.0 Len: 126 Query Start:1 Query End:126 Subject Strand: NEGATIVE Subject Start: 337 Subject End: 462 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgct......................................................................................... gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgct......................................................................................... Subject: smit_SMT_1985_SMT_1986|hypothetical protein predicted by Glimmer/Critica:mercuric reductase|NEGATIVE:POSITIVE|[1914662,1914982]|321 HSP 1 e-value: 4.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 162 Subject End: 282 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcc.............................................................................................. gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcc.............................................................................................. Subject: smit_SMT_2001_SMT_2002|membrane protease protein family:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1923950,1924234]|285 HSP 1 e-value: 4.0E-57 bit: 222.0 Len: 120 Query Start:1 Query End:120 Subject Strand: NEGATIVE Subject Start: 150 Subject End: 269 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttc............................................................................................... gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcgggttgcgtattgggcgctcttccgcttc............................................................................................... Subject: smit_SMT_2000_SMT_2001|hypothetical protein:membrane protease protein family|NEGATIVE:POSITIVE|[1923199,1923526]|328 HSP 1 e-value: 2.0E-55 bit: 216.0 Len: 113 Query Start:1 Query End:113 Subject Strand: NEGATIVE Subject Start: 143 Subject End: 255 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctctt...................................................................................................... gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctctt...................................................................................................... Subject: smit_SMT_387_SMT_388|hypothetical protein predicted by Glimmer/Critica:branched-chain amino acid transport system II carrier protein|POSITIVE:POSITIVE|[321179,321311]|133 HSP 1 e-value: 1.0E-53 bit: 210.0 Len: 110 Query Start:1 Query End:110 Subject Strand: NEGATIVE Subject Start: 6 Subject End: 115 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgct......................................................................................................... gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgct......................................................................................................... Subject: smit_SMT_1955_SMT_1956|carbamoyl-phosphate synthase small chain:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:NEGATIVE|[1889499,1889626]|128 HSP 1 e-value: 6.0E-53 bit: 208.0 Len: 105 Query Start:1 Query End:105 Subject Strand: POSITIVE Subject Start: 19 Subject End: 123 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgg.............................................................................................................. gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgg.............................................................................................................. Subject: smit_SMT_2044_SMT_2045|transcriptional activator TipA, putative:K+ transport system, NAD-binding component|NEGATIVE:POSITIVE|[1955976,1956278]|303 HSP 1 e-value: 1.0E-41 bit: 170.0 Len: 106 Query Start:1 Query End:106 Subject Strand: POSITIVE Subject Start: 98 Subject End: 203 gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattggg............................................................................................................. gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaaatcgcccacgcgcggggagaggcggtttgcgtattggg............................................................................................................. Subject: smit_SMT_2005_SMT_2006|pullulanase, type I:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1926380,1926611]|232 HSP 1 e-value: 2.0E-37 bit: 157.0 Len: 83 Query Start:77 Query End:159 Subject Strand: POSITIVE Subject Start: 1 Subject End: 83 ............................................................................cgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ ............................................................................cgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg........................................................ HSP 2 e-value: 2.0E-6 bit: 54.0 Len: 27 Query Start:1 Query End:27 Subject Strand: NEGATIVE Subject Start: 109 Subject End: 135 gtgaccatcctagcacagggggatcct............................................................................................................................................................................................ gtgaccatcctagcacagggggatcct............................................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUGACCAUCCUAGCACAGGGGGAUCCUCUAGAGUCGACCUGCAGGCAUGCAAGCUCUGCAUUAAUGAAUCGGCCAACGCGCGGGGAGAAGCGGUUUGCGUAUUGGGCGCUCUUCCGCUUCCUCGCUCACUGACUCGCUGCGCUCGGUCGUUCGGCUGCGNNNNNUUAAUUAAUUAANNNNNUACCAGGCGAAAGGACCAUACACAGUGAAACCAG (((........(((.((((((....)))..((((((...(((((((......)).))))).................(.(((((((..(((((...((((.....))))....)))))))))))).)..)))))).))).))).((((.((((.(((.......((((....))))........))).))))..))))...)))........... (-68.56) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUGGUUUCACUGUGUAUGGUCCUUUCGCCUGGUANNNNNUUAAUUAAUUAANNNNNCGCAGCCGAACGACCGAGCGCAGCGAGUCAGUGAGCGAGGAAGCGGAAGAGCGCCCAAUACGCAAACCGCUUCUCCCCGCGCGUUGGCCGAUUCAUUAAUGCAGAGCUUGCAUGCCUGCAGGUCGACUCUAGAGGAUCCCCCUGUGCUAGGAUGGUCAC (((..(.(.((((((.(((((..((((.(((........((((....)))).......))).)))).))))).)))))).))..)))...(((.((((((((....(((.......)))...))))))))...)))..(((((((..........(((((.((......)))))))))))))).......((((..((((...))))..)))).. (-67.76) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1166 150 101 165 116 smit:SMT_2005|5end_pullulanase,_type_I_1926289..1926519_NEGATIVE 1127 140 91 223 174 smit:SMT_304|5end_hypothetical_protein_246580..246810_POSITIVE 1112 120 71 229 180 smit:SMT_1967|5end_dicarboxylate/amino_acid:cation_(Na+_or_H+)_symporter_(DAACS)_family_protein_1899683..1899913_NEGATIVE 1112 120 71 229 180 smit:SMT_1690|5end_K06148_ATP-binding_cassette,_subfamily_C,_bacterial_1674779..1675009_NEGATIVE 1112 120 71 229 180 smit:SMT_779|5end_ribonuclease_BN,_putative_688530..688760_NEGATIVE 1111 50 1 223 174 smit:SMT_2016|5end_tyrosine_recombinase_1933425..1933655_NEGATIVE 1111 50 1 196 147 smit:SMT_2008|5end_K07485_transposase_1928662..1928892_NEGATIVE 1111 50 1 214 165 smit:SMT_2001|5end_membrane_protease_protein_family_1923387..1923617_POSITIVE 1111 50 1 159 110 smit:SMT_1961|5end_K07485_transposase_1894206..1894436_POSITIVE 1111 50 1 159 110 smit:SMT_1955|5end_carbamoyl-phosphate_synthase_small_chain_1889408..1889638_NEGATIVE 1111 50 1 203 154 smit:SMT_1954|5end_6-phospho-beta-glucosidase_1888548..1888778_POSITIVE 1111 50 1 194 145 smit:SMT_1944|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1879494..1879724_NEGATIVE 1111 50 1 208 159 smit:SMT_1155|5end_ABC-type_oligopeptide_transport_system,_periplasmic_component_1099665..1099895_NEGATIVE 1111 50 1 80 31 smit:SMT_464|5end_ribonuclease_Z_402867..403097_POSITIVE 1111 50 1 159 110 smit:SMT_388|5end_branched-chain_amino_acid_transport_system_II_carrier_protein_321172..321402_POSITIVE 1020 46 1 231 186 smit:SMT_1686|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1670175..1670405_POSITIVE 924 43 1 231 189 smit:SMT_2044|5end_transcriptional_activator_TipA,_putative_1955885..1956115_NEGATIVE 553 120 78 151 106 smit:SMT_2052|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1961591..1961821_NEGATIVE 546 27 1 215 189 smit:SMT_2006|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1926472..1926702_POSITIVE 406 64 23 69 14 smit:SMT_1785|5end_phenylalanyl-tRNA_synthetase_beta_chain_1763277..1763507_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1110 159 114 46 1 smit:SMT_780|3end_peptidase_family_U32_688752..688902_NEGATIVE 1087 90 41 70 21 smit:SMT_1956|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1889537..1889687_NEGATIVE 1063 80 31 55 6 smit:SMT_387|3end_hypothetical_protein_predicted_by_Glimmer/Critica_321118..321268_POSITIVE 413 100 79 22 1 smit:SMT_463|3end_para-aminobenzoate_synthetase_component_I_/_4-amino-4-deoxychorismate_lyase_402839..402989_POSITIVE 406 64 23 111 56 smit:SMT_1786|3end_phenylalanyl-tRNA_synthetase_beta_chain_1763235..1763385_NEGATIVE 386 155 116 84 25 smit:SMT_1941|3end_hypothetical_protein_1877663..1877813_POSITIVE 386 59 13 62 2 smit:SMT_1750|3end_anthranilate_phosphoribosyltransferase_1729095..1729245_POSITIVE 380 118 71 46 1 smit:SMT_2016|3end_tyrosine_recombinase_1933021..1933171_NEGATIVE 380 118 71 56 11 smit:SMT_2001|3end_membrane_protease_protein_family_1923889..1924039_POSITIVE 380 118 71 46 1 smit:SMT_1957|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1890829..1890979_POSITIVE 380 118 71 46 1 smit:SMT_1886|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1841198..1841348_POSITIVE 380 118 71 46 1 smit:SMT_1658|3end_hypothetical_protein_1648937..1649087_POSITIVE 380 118 71 46 1 smit:SMT_1558|3end_hypothetical_protein_1543855..1544005_NEGATIVE 380 118 71 46 1 smit:SMT_1155|3end_ABC-type_oligopeptide_transport_system,_periplasmic_component_1099183..1099333_NEGATIVE 379 157 111 139 82 smit:SMT_977|3end_conserved_hypothetical_protein,_Rrf2_family_protein_925252..925402_NEGATIVE 366 118 71 46 1 smit:SMT_2044|3end_transcriptional_activator_TipA,_putative_1955274..1955424_NEGATIVE 362 59 13 138 86 smit:SMT_1693|3end_dimethyladenosine_transferase_1676752..1676902_POSITIVE 358 59 13 62 6 smit:SMT_1559|3end_6-phosphofructokinase_1544614..1544764_NEGATIVE 354 125 82 57 10 smit:SMT_1181|3end_phosphotransferase_system,_mannose/fructose-specific_component_IIA_1139413..1139563_NEGATIVE 351 155 114 79 24 smit:SMT_955|3end_acyltransferase_family_protein_904703..904853_POSITIVE