Origin IGS: agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattggnnnnn .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 nnnnnccaatacgcaaaccgcttctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Mask Tandem Repeat Region ================================================ agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattggnnnnn Find is-nt database================================================ Query_seq: SMT_1955:SMT_1956|SMT_1955:SMT_1956:carbamoyl-phosphate synthase small chain:hypothetical protein predicted by Glimmer/Critica:<-<-:1889499..1889626 128 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattggnnnnn Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1955:SMT_1956|SMT_1955:SMT_1956:carbamoyl-phosphate synthase small chain:hypothetical protein predicted by Glimmer/Critica:<-<-:1889499..1889626 128 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattggnnnnn Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1955:SMT_1956|SMT_1955:SMT_1956:carbamoyl-phosphate synthase small chain:hypothetical protein predicted by Glimmer/Critica:<-<-:1889499..1889626 128 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattggnnnnn Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattggnnnnn Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 19 End: 126 .................. M T I L A Q G D P L E S T C R H A S S A L M N R P T R G E K R F A Y W X .. Protein_Len: 42 Strand: + Start: 3 End: 128 .. M F P D L G D H P S T G G S S R V D L Q A C K L C I N E S A N A R G E A V C V L X X Protein_Len: 31 Strand: - Start: 2 End: 94 . S N G S R P S W G L V P P D E L T S R C A H L S Q M L S D A M .................................. agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattggnnnnn Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattggnnnnn Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1955:SMT_1956|SMT_1955:SMT_1956:carbamoyl-phosphate synthase small chain:hypothetical protein predicted by Glimmer/Critica:<-<-:1889499..1889626 128 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattggnnnnn Intra-Species Hit: Count: 35 Min: 1 Max: 121 Len: 121 Subject: smit_SMT_1955_SMT_1956|carbamoyl-phosphate synthase small chain:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:NEGATIVE|[1889499,1889626]|128 HSP 1 e-value: 9.0E-63 bit: 240.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 121 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... Subject: smit_SMT_2029_SMT_2030|acetyltransferase, GNAT family:chromosomal replication initiator protein DnaA|POSITIVE:NEGATIVE|[1944345,1944879]|535 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: NEGATIVE Subject Start: 360 Subject End: 480 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_2016_SMT_2017|tyrosine recombinase:exonuclease RexA|NEGATIVE:NEGATIVE|[1933516,1935951]|2436 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 65 Subject End: 185 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_2000_SMT_2001|hypothetical protein:membrane protease protein family|NEGATIVE:POSITIVE|[1923199,1923526]|328 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: NEGATIVE Subject Start: 153 Subject End: 273 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1985_SMT_1986|hypothetical protein predicted by Glimmer/Critica:mercuric reductase|NEGATIVE:POSITIVE|[1914662,1914982]|321 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 144 Subject End: 264 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1978_SMT_1979|hypothetical protein:possible cell surface protein|NEGATIVE:NEGATIVE|[1909657,1910371]|715 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: NEGATIVE Subject Start: 503 Subject End: 623 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1967_SMT_1968|dicarboxylate/amino acid:cation (Na+ or H+) symporter (DAACS) family protein:transcription-repair coupling factor|NEGATIVE:NEGATIVE|[1899774,1900040]|267 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 121 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1953_SMT_1954|hypothetical protein:6-phospho-beta- glucosidase|POSITIVE:POSITIVE|[1888168,1888687]|520 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: NEGATIVE Subject Start: 356 Subject End: 476 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1946_SMT_1947|sucrose-6-phosphate hydrolase:lysine decarboxylase|POSITIVE:NEGATIVE|[1881266,1882402]|1137 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 475 Subject End: 595 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1944_SMT_1945|hypothetical protein predicted by Glimmer/Critica:putative dihydrolipoamide S-acetyltransferase|NEGATIVE:POSITIVE|[1879585, 1880100]|516 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 36 Subject End: 156 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1895_SMT_1896|hypothetical protein predicted by Glimmer/Critica:lysine decarboxylase|POSITIVE:NEGATIVE|[1847463,1848162]|700 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 60 Subject End: 180 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1885_SMT_1886|hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1840148,1841123]|976 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: NEGATIVE Subject Start: 656 Subject End: 776 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1857_SMT_1858|hypothetical protein:K03497 chromosome partitioning protein, ParB family|POSITIVE:NEGATIVE|[1821206,1821776]|571 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 75 Subject End: 195 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1849_SMT_1850|hypothetical protein:K01421 putative membrane protein|POSITIVE:POSITIVE|[1813865,1815201]|1337 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: NEGATIVE Subject Start: 875 Subject End: 995 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1690_SMT_1691|K06148 ATP-binding cassette, subfamily C, bacterial:putative deoxyribonuclease|NEGATIVE:POSITIVE|[1674870, 1675117]|248 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 121 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1685_SMT_1686|phenylalanyl-tRNA synthetase alpha chain:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1668936,1670314]|1379 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: NEGATIVE Subject Start: 1183 Subject End: 1303 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_779_SMT_780|ribonuclease BN, putative:peptidase family U32|NEGATIVE:NEGATIVE|[688621,688841]|221 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 121 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_478_SMT_479|hypothetical protein predicted by Glimmer/Critica:K03308 neurotransmitter:Na+ symporter, NSS family|POSITIVE:NEGATIVE|[415318,415693]|376 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: NEGATIVE Subject Start: 205 Subject End: 325 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_387_SMT_388|hypothetical protein predicted by Glimmer/Critica:branched-chain amino acid transport system II carrier protein|POSITIVE:POSITIVE|[321179,321311]|133 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 121 Query Start:1 Query End:121 Subject Strand: NEGATIVE Subject Start: 13 Subject End: 133 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1966_SMT_1967|DNA polymerase III alpha subunit:dicarboxylate/amino acid:cation (Na+ or H+) symporter (DAACS) family protein|POSITIVE:NEGATIVE|[1898389,1899587]|1199 HSP 1 e-value: 3.0E-59 bit: 228.0 Len: 119 Query Start:3 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 119 ..ctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... ..ctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... HSP 2 e-value: 2.0E-17 bit: 89.7 Len: 49 Query Start:73 Query End:121 Subject Strand: POSITIVE Subject Start: 227 Subject End: 275 ........................................................................tctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... ........................................................................tctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1926_SMT_1927|mevalonate kinase:cell division protein FtsH|POSITIVE:NEGATIVE|[1868123,1868497]|375 HSP 1 e-value: 3.0E-59 bit: 228.0 Len: 119 Query Start:3 Query End:121 Subject Strand: NEGATIVE Subject Start: 257 Subject End: 375 ..ctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... ..ctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... HSP 2 e-value: 1.0E-52 bit: 206.0 Len: 108 Query Start:14 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 108 .............tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... .............tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1884_SMT_1885|hypothetical protein:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1839299,1839919]|621 HSP 1 e-value: 3.0E-59 bit: 228.0 Len: 119 Query Start:3 Query End:121 Subject Strand: NEGATIVE Subject Start: 503 Subject End: 621 ..ctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... ..ctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1873_SMT_1874|K02006 cobalt transport system ATP-binding protein:hypothetical protein|POSITIVE:NEGATIVE|[1832412,1832712]|301 HSP 1 e-value: 3.0E-59 bit: 228.0 Len: 119 Query Start:3 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 119 ..ctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... ..ctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_2001_SMT_2002|membrane protease protein family:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1923950,1924234]|285 HSP 1 e-value: 8.0E-57 bit: 220.0 Len: 119 Query Start:3 Query End:121 Subject Strand: NEGATIVE Subject Start: 167 Subject End: 285 ..ctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... ..ctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcgggttgcgtatt....... Subject: smit_SMT_1845_SMT_1846|K01191 alpha-mannosidase:acyl-ACP thioesterase, putative|POSITIVE:POSITIVE|[1810496,1811549]|1054 HSP 1 e-value: 8.0E-57 bit: 220.0 Len: 121 Query Start:1 Query End:121 Subject Strand: NEGATIVE Subject Start: 589 Subject End: 709 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggakaggcgsyttgcgtatt....... Subject: smit_SMT_1558_SMT_1559|hypothetical protein:6- phosphofructokinase|NEGATIVE:NEGATIVE|[1544149, 1544703]|555 HSP 1 e-value: 2.0E-54 bit: 212.0 Len: 113 Query Start:1 Query End:113 Subject Strand: POSITIVE Subject Start: 191 Subject End: 303 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtt............... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctcygcattaatgaatcggccaacgcgcggggagaggcggtt............... Subject: smit_SMT_2024_SMT_2025|hypothetical protein:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1940751,1941084]|334 HSP 1 e-value: 1.0E-52 bit: 206.0 Len: 108 Query Start:14 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 108 .............tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... .............tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1981_SMT_1982|K03696 ATP-dependent Clp protease ATP-binding subunit ClpC:conserved hypothetical protein|POSITIVE:POSITIVE|[1912929,1913175]|247 HSP 1 e-value: 1.0E-52 bit: 206.0 Len: 108 Query Start:14 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 108 .............tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... .............tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1942_SMT_1943|zinc metalloprotease ZmpB, truncated:alpha/beta hydrolase fold|POSITIVE:NEGATIVE|[1878445,1878659]|215 HSP 1 e-value: 1.0E-52 bit: 206.0 Len: 108 Query Start:14 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 108 .............tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... .............tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1887_SMT_1888|LicC protein:K02026 multiple sugar transport system permease protein|POSITIVE:POSITIVE|[1841801,1842227]|427 HSP 1 e-value: 1.0E-52 bit: 206.0 Len: 108 Query Start:14 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 108 .............tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... .............tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1971_SMT_1972|hypothetical protein predicted by Glimmer/Critica:uncharacterized conserved phage related protein|POSITIVE:POSITIVE|[1903587,1903801]|215 HSP 1 e-value: 5.0E-52 bit: 204.0 Len: 103 Query Start:19 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 103 ..................gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... ..................gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... Subject: smit_SMT_2044_SMT_2045|transcriptional activator TipA, putative:K+ transport system, NAD-binding component|NEGATIVE:POSITIVE|[1955976,1956278]|303 HSP 1 e-value: 8.0E-51 bit: 200.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 80 Subject End: 200 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaaatcgcccacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_1851_SMT_1852|ABC transporter, permease protein, putative:1,4-alpha-glucan branching enzyme|NEGATIVE:NEGATIVE|[1815812,1816556]|745 HSP 1 e-value: 1.0E-49 bit: 196.0 Len: 103 Query Start:19 Query End:121 Subject Strand: NEGATIVE Subject Start: 643 Subject End: 745 ..................gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... ..................gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_325_SMT_326|K02029 polar amino acid transport system permease protein:K02029 polar amino acid transport system permease protein|NEGATIVE:NEGATIVE|[263114,263306]|193 HSP 1 e-value: 1.0E-49 bit: 196.0 Len: 103 Query Start:19 Query End:121 Subject Strand: NEGATIVE Subject Start: 91 Subject End: 193 ..................gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... ..................gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Subject: smit_SMT_303_SMT_304|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[245819,246719]|901 HSP 1 e-value: 2.0E-48 bit: 192.0 Len: 101 Query Start:1 Query End:101 Subject Strand: POSITIVE Subject Start: 119 Subject End: 219 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcgg........................... agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcatttatgaatcggccaacgcgcgg........................... HSP 2 e-value: 7.0E-45 bit: 180.0 Len: 95 Query Start:27 Query End:121 Subject Strand: NEGATIVE Subject Start: 807 Subject End: 901 ..........................cctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtatt....... ..........................cctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt....... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGCUGUUUCCUGAUCUAGGUGACCAUCCUAGCACAGGGGGAUCCUCUAGAGUCGACCUGCAGGCAUGCAAGCUCUGCAUUAAUGAAUCGGCCAACGCGCGGGGAGAAGCGGUUUGCGUAUU .(((.(((((((.((((((.((((.((((.....)))))).)).))))))(((((..(((((((......)).)))))........))))).......))))))).)))............ (-35.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUACGCAAACCGCUUCUCCCCGCGCGUUGGCCGAUUCAUUAAUGCAGAGCUUGCAUGCCUGCAGGUCGACUCUAGAGGAUCCCCCUGUGCUAGGAUGGUCACCUAGAUCAGGAAACAGCU ............(((..(((......(((((((..........(((((.((......))))))))))))))(((((..((((..((((...))))..))))..)))))...)))...))). (-35.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1098 80 31 208 159 smit:SMT_2008|5end_K07485_transposase_1928662..1928892_NEGATIVE 1098 80 31 77 28 smit:SMT_2005|5end_pullulanase,_type_I_1926289..1926519_NEGATIVE 1098 80 31 226 177 smit:SMT_2001|5end_membrane_protease_protein_family_1923387..1923617_POSITIVE 1098 80 31 171 122 smit:SMT_1967|5end_dicarboxylate/amino_acid:cation_(Na+_or_H+)_symporter_(DAACS)_family_protein_1899683..1899913_NEGATIVE 1098 80 31 171 122 smit:SMT_1961|5end_K07485_transposase_1894206..1894436_POSITIVE 1098 80 31 171 122 smit:SMT_1955|5end_carbamoyl-phosphate_synthase_small_chain_1889408..1889638_NEGATIVE 1098 80 31 215 166 smit:SMT_1954|5end_6-phospho-beta-glucosidase_1888548..1888778_POSITIVE 1098 80 31 206 157 smit:SMT_1944|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1879494..1879724_NEGATIVE 1098 80 31 171 122 smit:SMT_1690|5end_K06148_ATP-binding_cassette,_subfamily_C,_bacterial_1674779..1675009_NEGATIVE 1098 80 31 220 171 smit:SMT_1155|5end_ABC-type_oligopeptide_transport_system,_periplasmic_component_1099665..1099895_NEGATIVE 1098 80 31 171 122 smit:SMT_779|5end_ribonuclease_BN,_putative_688530..688760_NEGATIVE 1098 80 31 92 43 smit:SMT_464|5end_ribonuclease_Z_402867..403097_POSITIVE 1098 80 31 171 122 smit:SMT_388|5end_branched-chain_amino_acid_transport_system_II_carrier_protein_321172..321402_POSITIVE 1098 80 31 145 96 smit:SMT_304|5end_hypothetical_protein_246580..246810_POSITIVE 1094 70 21 225 176 smit:SMT_2016|5end_tyrosine_recombinase_1933425..1933655_NEGATIVE 1071 60 11 230 181 smit:SMT_2044|5end_transcriptional_activator_TipA,_putative_1955885..1956115_NEGATIVE 1071 60 11 227 178 smit:SMT_1686|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1670175..1670405_POSITIVE 931 45 1 215 171 smit:SMT_2006|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1926472..1926702_POSITIVE 417 89 41 77 14 smit:SMT_1785|5end_phenylalanyl-tRNA_synthetase_beta_chain_1763277..1763507_NEGATIVE 393 49 2 185 123 smit:SMT_621|5end_K03346_replication_initiation_and_membrane_attachment_protein_528794..529024_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1086 100 51 57 8 smit:SMT_387|3end_hypothetical_protein_predicted_by_Glimmer/Critica_321118..321268_POSITIVE 417 89 41 119 56 smit:SMT_1786|3end_phenylalanyl-tRNA_synthetase_beta_chain_1763235..1763385_NEGATIVE 413 118 97 22 1 smit:SMT_463|3end_para-aminobenzoate_synthetase_component_I_/_4-amino-4-deoxychorismate_lyase_402839..402989_POSITIVE 386 77 31 62 2 smit:SMT_1750|3end_anthranilate_phosphoribosyltransferase_1729095..1729245_POSITIVE 362 77 31 138 86 smit:SMT_1693|3end_dimethyladenosine_transferase_1676752..1676902_POSITIVE 358 77 31 62 6 smit:SMT_1559|3end_6-phosphofructokinase_1544614..1544764_NEGATIVE 346 21 1 33 13 smit:SMT_619|3end_riboflavin_kinase_/_FMN_adenylyltransferase_528719..528869_POSITIVE 304 66 24 60 14 smit:SMT_1293|3end_K02051_sulfonate/nitrate/taurine_transport_system_substrate-binding_protein_1256063..1256213_POSITIVE 299 59 17 66 23 smit:SMT_980|3end_hypothetical_protein_928227..928377_POSITIVE 294 110 62 148 102 smit:SMT_1778|3end_ATP-dependent_RNA_helicase_1753611..1753761_NEGATIVE 292 70 23 67 11 smit:SMT_140|3end_NUDIX_family_hydrolase_91498..91648_POSITIVE 288 63 21 151 109 smit:SMT_2052|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1960662..1960812_NEGATIVE 287 78 35 81 31 smit:SMT_1655|3end_glucose-6-phosphate_1-dehydrogenase_1643534..1643684_NEGATIVE 286 118 71 139 85 smit:SMT_1570|3end_hypothetical_protein_1563250..1563400_NEGATIVE 285 53 12 84 38 smit:SMT_1854|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1818952..1819102_POSITIVE 282 77 34 61 9 smit:SMT_1484|3end_exonuclease_RexA_1464256..1464406_NEGATIVE 282 112 81 56 19 smit:SMT_957|3end_UDP-glucose_4-epimerase_904776..904926_NEGATIVE 282 112 81 141 104 smit:SMT_956|3end_hypothetical_protein_predicted_by_Glimmer/Critica_904691..904841_NEGATIVE 280 80 31 71 3 smit:SMT_2057|3end_cell_division_protein_FtsH_1966897..1967047_NEGATIVE 280 50 13 62 20 smit:SMT_757|3end_K06969_putative_SAM-dependent_methyltransferase_660581..660731_NEGATIVE