Origin IGS: attgattgattttgactgttttagcaggaattccttaaaaaacaagtttgaaatcttagttaaaaggcttttgtttcattatctttcatattttcttgaaaagttgattaatttcctgctttaaatttatgataaacgtttaacagttttttctcttttttaacataaaaggcttgcattttttatcgaaaacggttactattaaatgtgataagatttttggaggaatgaatga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tcattcattcctccaaaaatcttatcacatttaatagtaaccgttttcgataaaaaatgcaagccttttatgttaaaaaagagaaaaaactgttaaacgtttatcataaatttaaagcaggaaattaatcaacttttcaagaaaatatgaaagataatgaaacaaaagccttttaactaagatttcaaacttgttttttaaggaattcctgctaaaacagtcaaaatcaatcaat Mask Tandem Repeat Region ================================================ attgattgattttgactgttttagcaggaattccttaaaaaacaagtttgaaatcttagttaaaaggcttttgtttcattatctttcatattttcttgaaaagttgattaatttcctgctttaaatttatgataaacgtttaacagttttttctcttttttaacataaaaggcttgcattttttatcgaaaacggttactattaaatgtgataagatttttggaggaatgaatga Find is-nt database================================================ Query_seq: SMT_1945:SMT_1946|SMT_1945:SMT_1946:putative dihydrolipoamide S-acetyltransferase:sucrose-6-phosphate hydrolase:->->:1880707..1880941 235 attgattgattttgactgttttagcaggaattccttaaaaaacaagtttgaaatcttagttaaaaggcttttgtttcattatctttcatattttcttgaaaagttgattaatttcctgctttaaatttatgataaacgtttaacagttttttctcttttttaacataaaaggcttgcattttttatcgaaaacggttactattaaatgtgataagatttttggaggaatgaatga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1945:SMT_1946|SMT_1945:SMT_1946:putative dihydrolipoamide S-acetyltransferase:sucrose-6-phosphate hydrolase:->->:1880707..1880941 235 attgattgattttgactgttttagcaggaattccttaaaaaacaagtttgaaatcttagttaaaaggcttttgtttcattatctttcatattttcttgaaaagttgattaatttcctgctttaaatttatgataaacgtttaacagttttttctcttttttaacataaaaggcttgcattttttatcgaaaacggttactattaaatgtgataagatttttggaggaatgaatga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1945:SMT_1946|SMT_1945:SMT_1946:putative dihydrolipoamide S-acetyltransferase:sucrose-6-phosphate hydrolase:->->:1880707..1880941 235 attgattgattttgactgttttagcaggaattccttaaaaaacaagtttgaaatcttagttaaaaggcttttgtttcattatctttcatattttcttgaaaagttgattaatttcctgctttaaatttatgataaacgtttaacagttttttctcttttttaacataaaaggcttgcattttttatcgaaaacggttactattaaatgtgataagatttttggaggaatgaatga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 33 Max: 179 Len: 147 Subject: UniRef90_Q97PB7 Cluster: Hypothetical protein; n=2; Streptococcus pneumoniae|Rep: Hypothetical protein - Streptococcus pneumoniae HSP 1 e-value: 4.0E-7 bit: 55.8 Len: 147 Query Start:33 Query End:179 Subject Strand: null Subject Start: 1 Subject End: 49 ................................ M Q A F Y V K K E K K L L N V Y H K F K A G N * S T F Q E N M K D N E T K A F * L R F Q T C F L R ........................................................ ................................ M Q A F Y V K K E E K L S K D Y H K I N T G N Q S N F Y E N V K D N E I K Y F L T K V S N L F F K ........................................................ attgattgattttgactgttttagcaggaattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntttttatcgaaaacggttactattaaatgtgataagatttttggaggaatgaatga Predict ORF larger than 30AA ================================================ Protein_Len: 40 Strand: + Start: 2 End: 121 . M I D F D C F S R N S L K N K F E I L V K R L L F H Y L S Y F L E K L I N F L L .................................................................................................................. attgattgattttgactgttttagcaggaattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntttttatcgaaaacggttactattaaatgtgataagatttttggaggaatgaatga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attgattgattttgactgttttagcaggaattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntttttatcgaaaacggttactattaaatgtgataagatttttggaggaatgaatga Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1945:SMT_1946|SMT_1945:SMT_1946:putative dihydrolipoamide S-acetyltransferase:sucrose-6-phosphate hydrolase:->->:1880707..1880941 235 attgattgattttgactgttttagcaggaattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntttttatcgaaaacggttactattaaatgtgataagatttttggaggaatgaatga Intra-Species Hit: Count: 2 Min: 1 Max: 235 Len: 235 Subject: smit_SMT_1945_SMT_1946|putative dihydrolipoamide S-acetyltransferase:sucrose-6-phosphate hydrolase|POSITIVE:POSITIVE|[1880707,1880941]|235 HSP 1 e-value: 1.0E-23 bit: 111.0 Len: 56 Query Start:180 Query End:235 Subject Strand: POSITIVE Subject Start: 180 Subject End: 235 ...................................................................................................................................................................................tttttatcgaaaacggttactattaaatgtgataagatttttggaggaatgaatga ...................................................................................................................................................................................tttttatcgaaaacggttactattaaatgtgataagatttttggaggaatgaatga HSP 2 e-value: 2.0E-9 bit: 63.9 Len: 32 Query Start:1 Query End:32 Subject Strand: POSITIVE Subject Start: 1 Subject End: 32 attgattgattttgactgttttagcaggaatt........................................................................................................................................................................................................... attgattgattttgactgttttagcaggaatt........................................................................................................................................................................................................... Subject: smit_SMT_913_SMT_914|putative sucrose-specific PTS permease, enzyme II:sucrose-6-phosphate hydrolase|NEGATIVE:POSITIVE|[865900,866278]|379 HSP 1 e-value: 1.0E-23 bit: 111.0 Len: 56 Query Start:180 Query End:235 Subject Strand: POSITIVE Subject Start: 324 Subject End: 379 ...................................................................................................................................................................................tttttatcgaaaacggttactattaaatgtgataagatttttggaggaatgaatga ...................................................................................................................................................................................tttttatcgaaaacggttactattaaatgtgataagatttttggaggaatgaatga HSP 2 e-value: 2.0E-9 bit: 63.9 Len: 32 Query Start:1 Query End:32 Subject Strand: POSITIVE Subject Start: 145 Subject End: 176 attgattgattttgactgttttagcaggaatt........................................................................................................................................................................................................... attgattgattttgactgttttagcaggaatt........................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUGAUUGAUUUUGACUGUUUUAGCAGGAAUUCCUUAAAAAACAAGUUUGAAAUCUUAGUUAAAAGGCUUUUGUUUCAUUAUCUUUCAUAUUUUCUUGAAAAGUUGAUUAAUUUCCUGCUUUAAAUUUAUGAUAAACGUUUAACAGUUUUUUCUCUUUUUUAACAUAAAAGGCUUGCAUUUUUUAUCGAAAACGGUUACUAUUAAAUGUGAUAAGAUUUUUGGAGGAAUGAAUGA .......((....(((((((..((((((((....((((..(((.....((((((...((((....))))...)))))).....(((((........))))).)))..)))).)))))))).....................)))))))...)).((((((......))))))....((((((((..((((((..(((((........)))))....))))))))))))))..... (-38.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCAUUCAUUCCUCCAAAAAUCUUAUCACAUUUAAUAGUAACCGUUUUCGAUAAAAAAUGCAAGCCUUUUAUGUUAAAAAAGAGAAAAAACUGUUAAACGUUUAUCAUAAAUUUAAAGCAGGAAAUUAAUCAACUUUUCAAGAAAAUAUGAAAGAUAAUGAAACAAAAGCCUUUUAACUAAGAUUUCAAACUUGUUUUUUAAGGAAUUCCUGCUAAAACAGUCAAAAUCAAUCAAU ..........................((.(((((((((...((((((......)))))).....(((((.......))))).......))))))))).))...............((((((((.....(((..((((((........))))))....)))........((((..(((.(((........))))))....))))..))))))))...................... (-29.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 257 100 51 226 174 smit:SMT_1474|5end_K02003_1455170..1455400_POSITIVE 248 38 14 104 77 smit:SMT_761|5end_prephenate_dehydrogenase_667150..667380_NEGATIVE 248 38 14 104 77 smit:SMT_752|5end_prephenate_dehydrogenase_656791..657021_NEGATIVE 244 120 82 223 184 smit:SMT_1680|5end_hypothetical_protein_1663494..1663724_POSITIVE 230 52 13 63 31 smit:SMT_762|5end_cation-transporting_ATPase,_E1-E2_family_protein_667691..667921_NEGATIVE 230 158 113 72 14 smit:SMT_404|5end_hypothetical_protein_333386..333616_POSITIVE 225 27 1 45 17 smit:SMT_1521|5end_K02003_1501887..1502117_NEGATIVE 225 99 54 107 56 smit:SMT_293|5end_dTDP-4-dehydrorhamnose_reductase_235919..236149_POSITIVE 218 196 152 134 97 smit:SMT_1696|5end_hypothetical_protein_1678182..1678412_POSITIVE 218 96 65 183 153 smit:SMT_1442|5end_hypothetical_protein_1426328..1426558_NEGATIVE 216 89 65 151 127 smit:SMT_487|5end_transcriptional_regulator,_TetR_family_422380..422610_POSITIVE 214 59 21 34 1 smit:SMT_1367|5end_K02237_competence_protein_ComEA_1347522..1347752_NEGATIVE 214 178 138 202 160 smit:SMT_878|5end_excinuclease_ABC,_subunit_C_826249..826479_NEGATIVE 213 59 23 48 5 smit:SMT_1071|5end_hypothetical_protein_1010058..1010288_POSITIVE 213 50 3 205 158 smit:SMT_960|5end_putative_esterase_906818..907048_NEGATIVE 213 90 44 112 72 smit:SMT_792|5end_hypothetical_protein_predicted_by_Glimmer/Critica_702571..702801_NEGATIVE 210 160 112 211 165 smit:SMT_1618|5end_K02015_iron_complex_transport_system_permease_protein_1605449..1605679_NEGATIVE 210 207 162 216 174 smit:SMT_1165|5end_sensor_protein_CiaH_1111820..1112050_NEGATIVE 210 207 162 216 174 smit:SMT_539|5end_sensor_histidine_kinase_CiaH_455752..455982_POSITIVE 209 100 58 107 69 smit:SMT_1857|5end_hypothetical_protein_1820931..1821161_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 257 100 51 95 43 smit:SMT_1473|3end_hypothetical_protein_1455119..1455269_POSITIVE 248 38 14 94 67 smit:SMT_753|3end_chorismate_synthase_656801..656951_NEGATIVE 225 27 1 32 4 smit:SMT_1522|3end_K02004_1501900..1502050_NEGATIVE 221 119 73 123 73 smit:SMT_1203|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1164221..1164371_NEGATIVE 213 90 44 112 72 smit:SMT_793|3end_K02029_polar_amino_acid_transport_system_permease_protein_702571..702721_NEGATIVE 209 99 64 110 77 smit:SMT_2029|3end_acetyltransferase,_GNAT_family_1944284..1944434_POSITIVE 209 100 58 95 57 smit:SMT_1856|3end_polypeptide_deformylase_1820999..1821149_POSITIVE 209 99 64 110 77 smit:SMT_1755|3end_acetyltransferase,_GNAT_family_1733577..1733727_POSITIVE 209 100 58 95 57 smit:SMT_835|3end_polypeptide_deformylase_777124..777274_NEGATIVE 206 70 26 103 56 smit:SMT_1945|3end_putative_dihydrolipoamide_S-acetyltransferase_1880646..1880796_POSITIVE 206 104 62 125 70 smit:SMT_1609|3end_hypothetical_protein_1598654..1598804_POSITIVE 205 186 142 47 3 smit:SMT_1548|3end_cell_division_protein_FtsZ_1533720..1533870_POSITIVE 204 120 71 123 81 smit:SMT_1032|3end_putative_biotin_operon_repressor_969759..969909_POSITIVE 202 68 23 126 94 smit:SMT_1990|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1917677..1917827_POSITIVE 202 200 151 80 12 smit:SMT_1218|3end_DNA_polymerase_I_1176688..1176838_NEGATIVE 200 118 72 116 73 smit:SMT_288|3end_hypothetical_protein_232253..232403_POSITIVE 198 120 71 145 98 smit:SMT_772|3end_spermidine/putrescine_ABC_transporter,_ATP-binding_protein_682143..682293_NEGATIVE 198 129 81 98 54 smit:SMT_602|3end_5'-methylthioadenosine_nucleosidase_/_S-adenosylhomocysteine_nucleosidase_517964..518114_POSITIVE 197 119 78 55 17 smit:SMT_1643|3end_2-isopropylmalate_synthase_1630772..1630922_POSITIVE 197 119 72 70 13 smit:SMT_1498|3end_transcriptional_regulator,_LytR_family_1483093..1483243_POSITIVE