Origin IGS: atagttaaaggtttttcctgtgaccttatcgacctcttttccgccctcgaggtcccttgtctttccagcnnnnnttaattaattaannnnntcggggctggcttaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttaagccagccccgannnnnttaattaattaannnnngctggaaagacaagggacctcgagggcggaaaagaggtcgataaggtcacaggaaaaacctttaactat Mask Tandem Repeat Region ================================================ atagttaaaggtttttcctgtgaccttatcgacctcttttccgccctcgaggtcccttgtctttccagcnnnnnttaattaattaannnnntcggggctggcttaa Find is-nt database================================================ Query_seq: SMT_1943:SMT_1944|SMT_1943:SMT_1944:alpha/beta hydrolase fold:hypothetical protein predicted by Glimmer/Critica:<-<-:1879059..1879164 106 atagttaaaggtttttcctgtgaccttatcgacctcttttccgccctcgaggtcccttgtctttccagcnnnnnttaattaattaannnnntcggggctggcttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1943:SMT_1944|SMT_1943:SMT_1944:alpha/beta hydrolase fold:hypothetical protein predicted by Glimmer/Critica:<-<-:1879059..1879164 106 atagttaaaggtttttcctgtgaccttatcgacctcttttccgccctcgaggtcccttgtctttccagcnnnnnttaattaattaannnnntcggggctggcttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1943:SMT_1944|SMT_1943:SMT_1944:alpha/beta hydrolase fold:hypothetical protein predicted by Glimmer/Critica:<-<-:1879059..1879164 106 atagttaaaggtttttcctgtgaccttatcgacctcttttccgccctcgaggtcccttgtctttccagcnnnnnttaattaattaannnnntcggggctggcttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atagttaaaggtttttcctgtgaccttatcgacctcttttccgccctcgaggtcccttgtctttccagcnnnnnttaattaattaannnnntcggggctggcttaa Predict ORF larger than 30AA ================================================ atagttaaaggtttttcctgtgaccttatcgacctcttttccgccctcgaggtcccttgtctttccagcnnnnnttaattaattaannnnntcggggctggcttaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atagttaaaggtttttcctgtgaccttatcgacctcttttccgccctcgaggtcccttgtctttccagcnnnnnttaattaattaannnnntcggggctggcttaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1943:SMT_1944|SMT_1943:SMT_1944:alpha/beta hydrolase fold:hypothetical protein predicted by Glimmer/Critica:<-<-:1879059..1879164 106 atagttaaaggtttttcctgtgaccttatcgacctcttttccgccctcgaggtcccttgtctttccagcnnnnnttaattaattaannnnntcggggctggcttaa Intra-Species Hit: Count: 1 Min: 1 Max: 106 Len: 106 Subject: smit_SMT_1943_SMT_1944|alpha/beta hydrolase fold:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:NEGATIVE|[1879059,1879164]|106 HSP 1 e-value: 5.0E-36 bit: 151.0 Len: 106 Query Start:1 Query End:106 Subject Strand: POSITIVE Subject Start: 1 Subject End: 106 atagttaaaggtttttcctgtgaccttatcgacctcttttccgccctcgaggtcccttgtctttccagcnnnnnttaattaattaannnnntcggggctggcttaa atagttaaaggtttttcctgtgaccttatcgacctcttttccgccctcgaggtcccttgtctttccagcnnnnnttaattaattaannnnntcggggctggcttaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAGUUAAAGGUUUUUCCUGUGACCUUAUCGACCUCUUUUCCGCCCUCGAGGUCCCUUGUCUUUCCAGCNNNNNUUAAUUAAUUAANNNNNUCGGGGCUGGCUUAA .......((((((........))))))...((((((............))))))....(((..(((........((((....))))........)))..))).... (-18.69) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAAGCCAGCCCCGANNNNNUUAAUUAAUUAANNNNNGCUGGAAAGACAAGGGACCUCGAGGGCGGAAAAGAGGUCGAUAAGGUCACAGGAAAAACCUUUAACUAU .....(((((...........................)))))...(((....((((((............))))))......)))..(((.....)))........ (-18.33) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1019 69 21 160 112 smit:SMT_1943|5end_alpha/beta_hydrolase_fold_1878968..1879198_NEGATIVE 357 56 15 204 158 smit:SMT_1711|5end_Xaa-Pro_dipeptidase_1697494..1697724_NEGATIVE 357 69 24 89 43 smit:SMT_1481|5end_endopeptidase_O_1462229..1462459_POSITIVE 333 69 21 109 62 smit:SMT_446|5end_ABC-type_cobalt_transport_system,_ATPase_component_384870..385100_POSITIVE 321 69 22 154 97 smit:SMT_382|5end_valyl-tRNA_synthetase_317706..317936_POSITIVE 317 50 9 51 2 smit:SMT_1077|5end_GTP_cyclohydrolase_I_1015410..1015640_NEGATIVE 309 46 3 101 61 smit:SMT_1473|5end_hypothetical_protein_1454320..1454550_POSITIVE 303 57 13 207 163 smit:SMT_929|5end_hypothetical_protein_883342..883572_NEGATIVE 299 64 33 125 92 smit:SMT_977|5end_conserved_hypothetical_protein,_Rrf2_family_protein_925689..925919_NEGATIVE 287 69 24 164 115 smit:SMT_148|5end_glycoprotein_endopeptidase_98033..98263_POSITIVE 286 68 33 47 10 smit:SMT_1529|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1512880..1513110_NEGATIVE 280 57 14 80 33 smit:SMT_1915|5end_pyridoxine_biosynthesis_protein_1860329..1860559_POSITIVE 280 65 22 74 34 smit:SMT_218|5end_undecaprenyl_pyrophosphate_synthetase_150155..150385_POSITIVE 277 69 21 181 124 smit:SMT_1897|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1848858..1849088_NEGATIVE 277 84 41 231 187 smit:SMT_1788|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1765029..1765259_NEGATIVE 275 68 21 140 83 smit:SMT_1189|5end_phosphoribosylaminoimidazole_carboxylase_ATPase_subunit_1147754..1147984_NEGATIVE 272 86 41 175 124 smit:SMT_580|5end_K04094_glucose_inhibited_division_protein_Gid_497421..497651_POSITIVE 270 68 21 210 169 smit:SMT_1853|5end_1,4-alpha-glucan_branching_enzyme_1818064..1818294_NEGATIVE 269 48 3 179 116 smit:SMT_1768|5end_hydrolase,_alpha/beta_fold_family_1744959..1745189_NEGATIVE 268 69 25 134 87 smit:SMT_908|5end_hypothetical_protein_857230..857460_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 333 69 21 108 61 smit:SMT_445|3end_hypothetical_protein_384949..385099_POSITIVE 324 69 25 46 1 smit:SMT_1480|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1462266..1462416_POSITIVE 317 50 9 70 21 smit:SMT_1078|3end_folylpolyglutamate_synthase_1015391..1015541_NEGATIVE 315 49 5 67 16 smit:SMT_1443|3end_23S_rRNA_m(5)U_1939_methyltransferase_1426503..1426653_NEGATIVE 308 86 42 58 8 smit:SMT_2005|3end_pullulanase,_type_I_1925615..1925765_NEGATIVE 299 64 33 49 16 smit:SMT_978|3end_hypothetical_protein_925765..925915_NEGATIVE 295 86 42 102 58 smit:SMT_1941|3end_hypothetical_protein_1877663..1877813_POSITIVE 287 69 24 67 18 smit:SMT_147|3end_K02027_multiple_sugar_transport_system_substrate-binding_protein_98016..98166_POSITIVE 286 68 33 151 114 smit:SMT_1529|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1512776..1512926_NEGATIVE 284 67 28 134 94 smit:SMT_1912|3end_D-alanyl-D-alanine_carboxypeptidase_1857807..1857957_NEGATIVE 284 67 28 134 94 smit:SMT_868|3end_D-alanyl-D-alanine_carboxypeptidase_816509..816659_NEGATIVE 273 69 23 81 30 smit:SMT_1069|3end_acetyl_xylan_esterase,_putative_1009595..1009745_POSITIVE 272 86 41 102 51 smit:SMT_579|3end_ribosomal_large_subunit_pseudouridine_synthase_D_497428..497578_POSITIVE 269 49 9 69 25 smit:SMT_1920|3end_ABC_transporter,_ATP-binding_protein_1862736..1862886_NEGATIVE 268 69 25 141 94 smit:SMT_909|3end_type_I_restriction_enzyme_M_protein_857223..857373_NEGATIVE 266 69 30 147 104 smit:SMT_1348|3end_exoribonuclease_II_1325148..1325298_NEGATIVE 264 61 21 151 96 smit:SMT_1190|3end_phosphoribosylaminoimidazole_carboxylase,_catalytic_subunit_1147741..1147891_NEGATIVE 263 57 12 73 21 smit:SMT_1256|3end_ketol-acid_reductoisomerase_1221855..1222005_NEGATIVE 261 48 3 141 94 smit:SMT_561|3end_putative_translation_elongation_factor_EF-Tu_480687..480837_POSITIVE 257 67 31 145 108 smit:SMT_930|3end_ATP-dependent_RNA_helicase_883389..883539_NEGATIVE