Origin IGS: tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgannnnnttaattaattaannnnngtggctggaagccgataccgtcagtagcacctgttctcagaattgttttgaccata .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tatggtcaaaacaattctgagaacaggtgctactgacggtatcggcttccagccacnnnnnttaattaattaannnnntcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga Mask Tandem Repeat Region ================================================ tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgannnnnttaattaattaannnnngtggctggaagccgataccgtcagtagcacctgttctcagaattgttttgaccata Find is-nt database================================================ Query_seq: SMT_1942:SMT_1943|SMT_1942:SMT_1943:zinc metalloprotease ZmpB, truncated:alpha/beta hydrolase fold:-><-:1878445..1878659 215 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgannnnnttaattaattaannnnngtggctggaagccgataccgtcagtagcacctgttctcagaattgttttgaccata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1942:SMT_1943|SMT_1942:SMT_1943:zinc metalloprotease ZmpB, truncated:alpha/beta hydrolase fold:-><-:1878445..1878659 215 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgannnnnttaattaattaannnnngtggctggaagccgataccgtcagtagcacctgttctcagaattgttttgaccata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1942:SMT_1943|SMT_1942:SMT_1943:zinc metalloprotease ZmpB, truncated:alpha/beta hydrolase fold:-><-:1878445..1878659 215 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgannnnnttaattaattaannnnngtggctggaagccgataccgtcagtagcacctgttctcagaattgttttgaccata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgannnnnttaattaattaannnnngtggctggaagccgataccgtcagtagcacctgttctcagaattgttttgaccata Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: + Start: 6 End: 134 ..... M T I L A Q G D P L E S T C R H A S S A L M N R P T R G E R R F A Y W A L F R F L A H ................................................................................. Protein_Len: 36 Strand: + Start: 44 End: 151 ........................................... M Q A C K L C I N E S A N A R G E A V C V L G A L P L P R S L X X L I N ................................................................ Protein_Len: 49 Strand: - Start: 1 End: 147 R P S W G L V P P D E L T S R C A H L S Q M L S D A L A R P S A T Q T N P A R G S G R E S X X X M .................................................................... tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgannnnnttaattaattaannnnngtggctggaagccgataccgtcagtagcacctgttctcagaattgttttgaccata Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgannnnnttaattaattaannnnngtggctggaagccgataccgtcagtagcacctgttctcagaattgttttgaccata Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1942:SMT_1943|SMT_1942:SMT_1943:zinc metalloprotease ZmpB, truncated:alpha/beta hydrolase fold:-><-:1878445..1878659 215 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgannnnnttaattaattaannnnngtggctggaagccgataccgtcagtagcacctgttctcagaattgttttgaccata Intra-Species Hit: Count: 36 Min: 1 Max: 215 Len: 215 Subject: smit_SMT_1942_SMT_1943|zinc metalloprotease ZmpB, truncated:alpha/beta hydrolase fold|POSITIVE:NEGATIVE|[1878445,1878659]|215 HSP 1 e-value: 1.0E-100 bit: 367.0 Len: 215 Query Start:1 Query End:215 Subject Strand: POSITIVE Subject Start: 1 Subject End: 215 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgannnnnttaattaattaannnnngtggctggaagccgataccgtcagtagcacctgttctcagaattgttttgaccata tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgannnnnttaattaattaannnnngtggctggaagccgataccgtcagtagcacctgttctcagaattgttttgaccata Subject: smit_SMT_2024_SMT_2025|hypothetical protein:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1940751,1941084]|334 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 1 Subject End: 137 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_2016_SMT_2017|tyrosine recombinase:exonuclease RexA|NEGATIVE:NEGATIVE|[1933516,1935951]|2436 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 78 Subject End: 214 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1981_SMT_1982|K03696 ATP-dependent Clp protease ATP-binding subunit ClpC:conserved hypothetical protein|POSITIVE:POSITIVE|[1912929,1913175]|247 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 1 Subject End: 137 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1978_SMT_1979|hypothetical protein:possible cell surface protein|NEGATIVE:NEGATIVE|[1909657,1910371]|715 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: NEGATIVE Subject Start: 474 Subject End: 610 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1967_SMT_1968|dicarboxylate/amino acid:cation (Na+ or H+) symporter (DAACS) family protein:transcription-repair coupling factor|NEGATIVE:NEGATIVE|[1899774,1900040]|267 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 14 Subject End: 150 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1966_SMT_1967|DNA polymerase III alpha subunit:dicarboxylate/amino acid:cation (Na+ or H+) symporter (DAACS) family protein|POSITIVE:NEGATIVE|[1898389,1899587]|1199 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 12 Subject End: 148 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. HSP 2 e-value: 7.0E-37 bit: 155.0 Len: 78 Query Start:60 Query End:137 Subject Strand: POSITIVE Subject Start: 227 Subject End: 304 ...........................................................tctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. ...........................................................tctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1953_SMT_1954|hypothetical protein:6-phospho-beta- glucosidase|POSITIVE:POSITIVE|[1888168,1888687]|520 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: NEGATIVE Subject Start: 327 Subject End: 463 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1946_SMT_1947|sucrose-6-phosphate hydrolase:lysine decarboxylase|POSITIVE:NEGATIVE|[1881266,1882402]|1137 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 488 Subject End: 624 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1944_SMT_1945|hypothetical protein predicted by Glimmer/Critica:putative dihydrolipoamide S-acetyltransferase|NEGATIVE:POSITIVE|[1879585, 1880100]|516 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 49 Subject End: 185 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1926_SMT_1927|mevalonate kinase:cell division protein FtsH|POSITIVE:NEGATIVE|[1868123,1868497]|375 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 1 Subject End: 137 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. HSP 2 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: NEGATIVE Subject Start: 228 Subject End: 364 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1895_SMT_1896|hypothetical protein predicted by Glimmer/Critica:lysine decarboxylase|POSITIVE:NEGATIVE|[1847463,1848162]|700 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 73 Subject End: 209 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1887_SMT_1888|LicC protein:K02026 multiple sugar transport system permease protein|POSITIVE:POSITIVE|[1841801,1842227]|427 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 1 Subject End: 137 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1885_SMT_1886|hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1840148,1841123]|976 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: NEGATIVE Subject Start: 627 Subject End: 763 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1884_SMT_1885|hypothetical protein:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1839299,1839919]|621 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: NEGATIVE Subject Start: 474 Subject End: 610 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1873_SMT_1874|K02006 cobalt transport system ATP-binding protein:hypothetical protein|POSITIVE:NEGATIVE|[1832412,1832712]|301 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 12 Subject End: 148 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1857_SMT_1858|hypothetical protein:K03497 chromosome partitioning protein, ParB family|POSITIVE:NEGATIVE|[1821206,1821776]|571 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 88 Subject End: 224 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1849_SMT_1850|hypothetical protein:K01421 putative membrane protein|POSITIVE:POSITIVE|[1813865,1815201]|1337 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: NEGATIVE Subject Start: 846 Subject End: 982 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1690_SMT_1691|K06148 ATP-binding cassette, subfamily C, bacterial:putative deoxyribonuclease|NEGATIVE:POSITIVE|[1674870, 1675117]|248 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 14 Subject End: 150 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1685_SMT_1686|phenylalanyl-tRNA synthetase alpha chain:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1668936,1670314]|1379 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: NEGATIVE Subject Start: 1154 Subject End: 1290 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_779_SMT_780|ribonuclease BN, putative:peptidase family U32|NEGATIVE:NEGATIVE|[688621,688841]|221 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 14 Subject End: 150 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_478_SMT_479|hypothetical protein predicted by Glimmer/Critica:K03308 neurotransmitter:Na+ symporter, NSS family|POSITIVE:NEGATIVE|[415318,415693]|376 HSP 1 e-value: 5.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: NEGATIVE Subject Start: 176 Subject End: 312 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1851_SMT_1852|ABC transporter, permease protein, putative:1,4-alpha-glucan branching enzyme|NEGATIVE:NEGATIVE|[1815812,1816556]|745 HSP 1 e-value: 4.0E-69 bit: 262.0 Len: 132 Query Start:6 Query End:137 Subject Strand: NEGATIVE Subject Start: 614 Subject End: 745 .....gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. .....gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_325_SMT_326|K02029 polar amino acid transport system permease protein:K02029 polar amino acid transport system permease protein|NEGATIVE:NEGATIVE|[263114,263306]|193 HSP 1 e-value: 4.0E-69 bit: 262.0 Len: 132 Query Start:6 Query End:137 Subject Strand: NEGATIVE Subject Start: 62 Subject End: 193 .....gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. .....gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_2029_SMT_2030|acetyltransferase, GNAT family:chromosomal replication initiator protein DnaA|POSITIVE:NEGATIVE|[1944345,1944879]|535 HSP 1 e-value: 2.0E-68 bit: 260.0 Len: 131 Query Start:1 Query End:131 Subject Strand: NEGATIVE Subject Start: 337 Subject End: 467 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgct.................................................................................... tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgct.................................................................................... Subject: smit_SMT_1845_SMT_1846|K01191 alpha-mannosidase:acyl-ACP thioesterase, putative|POSITIVE:POSITIVE|[1810496,1811549]|1054 HSP 1 e-value: 2.0E-68 bit: 260.0 Len: 137 Query Start:1 Query End:137 Subject Strand: NEGATIVE Subject Start: 560 Subject End: 696 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggakaggcgsyttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1971_SMT_1972|hypothetical protein predicted by Glimmer/Critica:uncharacterized conserved phage related protein|POSITIVE:POSITIVE|[1903587,1903801]|215 HSP 1 e-value: 1.0E-66 bit: 254.0 Len: 132 Query Start:6 Query End:137 Subject Strand: POSITIVE Subject Start: 1 Subject End: 132 .....gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. .....gtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1558_SMT_1559|hypothetical protein:6- phosphofructokinase|NEGATIVE:NEGATIVE|[1544149, 1544703]|555 HSP 1 e-value: 4.0E-66 bit: 252.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 204 Subject End: 339 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctcygcattaatgaatcggccaacgcgcggggagaggcgg-ttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. Subject: smit_SMT_1985_SMT_1986|hypothetical protein predicted by Glimmer/Critica:mercuric reductase|NEGATIVE:POSITIVE|[1914662,1914982]|321 HSP 1 e-value: 2.0E-65 bit: 250.0 Len: 126 Query Start:1 Query End:126 Subject Strand: POSITIVE Subject Start: 157 Subject End: 282 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcc......................................................................................... tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcc......................................................................................... Subject: smit_SMT_303_SMT_304|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[245819,246719]|901 HSP 1 e-value: 3.0E-64 bit: 246.0 Len: 124 Query Start:14 Query End:137 Subject Strand: NEGATIVE Subject Start: 778 Subject End: 901 .............cctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. .............cctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. HSP 2 e-value: 2.0E-40 bit: 167.0 Len: 88 Query Start:1 Query End:88 Subject Strand: POSITIVE Subject Start: 132 Subject End: 219 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcgg............................................................................................................................... tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcatttatgaatcggccaacgcgcgg............................................................................................................................... Subject: smit_SMT_2001_SMT_2002|membrane protease protein family:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1923950,1924234]|285 HSP 1 e-value: 2.0E-62 bit: 240.0 Len: 125 Query Start:1 Query End:125 Subject Strand: NEGATIVE Subject Start: 150 Subject End: 274 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttc.......................................................................................... tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcgggttgcgtattgggcgctcttccgcttc.......................................................................................... Subject: smit_SMT_2000_SMT_2001|hypothetical protein:membrane protease protein family|NEGATIVE:POSITIVE|[1923199,1923526]|328 HSP 1 e-value: 1.0E-60 bit: 234.0 Len: 118 Query Start:1 Query End:118 Subject Strand: NEGATIVE Subject Start: 143 Subject End: 260 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctctt................................................................................................. tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctctt................................................................................................. Subject: smit_SMT_387_SMT_388|hypothetical protein predicted by Glimmer/Critica:branched-chain amino acid transport system II carrier protein|POSITIVE:POSITIVE|[321179,321311]|133 HSP 1 e-value: 6.0E-59 bit: 228.0 Len: 115 Query Start:1 Query End:115 Subject Strand: NEGATIVE Subject Start: 6 Subject End: 120 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgct.................................................................................................... tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgct.................................................................................................... Subject: smit_SMT_1955_SMT_1956|carbamoyl-phosphate synthase small chain:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:NEGATIVE|[1889499,1889626]|128 HSP 1 e-value: 1.0E-53 bit: 210.0 Len: 110 Query Start:1 Query End:110 Subject Strand: POSITIVE Subject Start: 14 Subject End: 123 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgg......................................................................................................... tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaagcggtttgcgtattgg......................................................................................................... Subject: smit_SMT_2044_SMT_2045|transcriptional activator TipA, putative:K+ transport system, NAD-binding component|NEGATIVE:POSITIVE|[1955976,1956278]|303 HSP 1 e-value: 5.0E-47 bit: 188.0 Len: 111 Query Start:1 Query End:111 Subject Strand: POSITIVE Subject Start: 93 Subject End: 203 tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattggg........................................................................................................ tctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaaatcgcccacgcgcggggagaggcggtttgcgtattggg........................................................................................................ Subject: smit_SMT_2005_SMT_2006|pullulanase, type I:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1926380,1926611]|232 HSP 1 e-value: 1.0E-23 bit: 111.0 Len: 56 Query Start:82 Query End:137 Subject Strand: POSITIVE Subject Start: 1 Subject End: 56 .................................................................................cgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. .................................................................................cgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga.............................................................................. HSP 2 e-value: 2.0E-9 bit: 63.9 Len: 32 Query Start:1 Query End:32 Subject Strand: NEGATIVE Subject Start: 109 Subject End: 140 tctaggtgaccatcctagcacagggggatcct....................................................................................................................................................................................... tctaggtgaccatcctagcacagggggatcct....................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUAGGUGACCAUCCUAGCACAGGGGGAUCCUCUAGAGUCGACCUGCAGGCAUGCAAGCUCUGCAUUAAUGAAUCGGCCAACGCGCGGGGAGAGGCGGUUUGCGUAUUGGGCGCUCUUCCGCUUCCUCGCUCACUGANNNNNUUAAUUAAUUAANNNNNGUGGCUGGAAGCCGAUACCGUCAGUAGCACCUGUUCUCAGAAUUGUUUUGACCAUA ((((((.((((.((((.....)))))).)).))))))((((...(((((((......)).)))))........(((((((..(.(((((((..(((((...((((.....))))....)))))))))))).)...........(((....))).......)))))))....))))...(((((.((((.(((....)))...))))))))).... (-65.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUGGUCAAAACAAUUCUGAGAACAGGUGCUACUGACGGUAUCGGCUUCCAGCCACNNNNNUUAAUUAAUUAANNNNNUCAGUGAGCGAGGAAGCGGAAGAGCGCCCAAUACGCAAACCGCCUCUCCCCGCGCGUUGGCCGAUUCAUUAAUGCAGAGCUUGCAUGCCUGCAGGUCGACUCUAGAGGAUCCCCCUGUGCUAGGAUGGUCACCUAGA ..(((((((.........((((.(((......))).((((.((.((((((.(((((.....((((....))))........))).))..)))))).))...(((.......)))..))))..))))........))))))).........(((((.((......))))))).......(((((..((((..((((...))))..))))..))))) (-59.85) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1200 130 81 140 91 smit:SMT_2005|5end_pullulanase,_type_I_1926289..1926519_NEGATIVE 1200 130 81 208 159 smit:SMT_304|5end_hypothetical_protein_246580..246810_POSITIVE 1166 120 71 224 175 smit:SMT_1967|5end_dicarboxylate/amino_acid:cation_(Na+_or_H+)_symporter_(DAACS)_family_protein_1899683..1899913_NEGATIVE 1166 120 71 224 175 smit:SMT_1690|5end_K06148_ATP-binding_cassette,_subfamily_C,_bacterial_1674779..1675009_NEGATIVE 1166 120 71 224 175 smit:SMT_779|5end_ribonuclease_BN,_putative_688530..688760_NEGATIVE 1109 90 41 231 182 smit:SMT_2008|5end_K07485_transposase_1928662..1928892_NEGATIVE 1109 90 41 194 145 smit:SMT_1955|5end_carbamoyl-phosphate_synthase_small_chain_1889408..1889638_NEGATIVE 1109 90 41 229 180 smit:SMT_1944|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1879494..1879724_NEGATIVE 1109 90 41 115 66 smit:SMT_464|5end_ribonuclease_Z_402867..403097_POSITIVE 1109 90 41 194 145 smit:SMT_388|5end_branched-chain_amino_acid_transport_system_II_carrier_protein_321172..321402_POSITIVE 1101 60 11 228 179 smit:SMT_2016|5end_tyrosine_recombinase_1933425..1933655_NEGATIVE 1101 60 11 219 170 smit:SMT_2001|5end_membrane_protease_protein_family_1923387..1923617_POSITIVE 1101 60 11 164 115 smit:SMT_1961|5end_K07485_transposase_1894206..1894436_POSITIVE 1101 60 11 208 159 smit:SMT_1954|5end_6-phospho-beta-glucosidase_1888548..1888778_POSITIVE 1101 60 11 213 164 smit:SMT_1155|5end_ABC-type_oligopeptide_transport_system,_periplasmic_component_1099665..1099895_NEGATIVE 1094 50 1 230 181 smit:SMT_1686|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1670175..1670405_POSITIVE 1032 48 1 231 184 smit:SMT_2044|5end_transcriptional_activator_TipA,_putative_1955885..1956115_NEGATIVE 671 210 163 149 99 smit:SMT_2031|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1945761..1945991_NEGATIVE 666 210 163 87 36 smit:SMT_1767|5end_oxidoreductase,_short_chain_dehydrogenase/reductase_family_1744121..1744351_NEGATIVE 654 32 1 215 184 smit:SMT_2006|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1926472..1926702_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1109 90 41 65 16 smit:SMT_1956|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1889537..1889687_NEGATIVE 1109 90 41 60 11 smit:SMT_387|3end_hypothetical_protein_predicted_by_Glimmer/Critica_321118..321268_POSITIVE 671 210 163 85 35 smit:SMT_2032|3end_hydrolase,_alpha/beta_fold_family_1945825..1945975_NEGATIVE 666 210 163 85 34 smit:SMT_1768|3end_hydrolase,_alpha/beta_fold_family_1744123..1744273_NEGATIVE 415 130 83 53 5 smit:SMT_2044|3end_transcriptional_activator_TipA,_putative_1955274..1955424_NEGATIVE 415 130 83 53 5 smit:SMT_2016|3end_tyrosine_recombinase_1933021..1933171_NEGATIVE 415 130 83 63 15 smit:SMT_2001|3end_membrane_protease_protein_family_1923889..1924039_POSITIVE 415 130 83 53 5 smit:SMT_1957|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1890829..1890979_POSITIVE 415 130 83 53 5 smit:SMT_1886|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1841198..1841348_POSITIVE 415 130 83 53 5 smit:SMT_1658|3end_hypothetical_protein_1648937..1649087_POSITIVE 415 130 83 53 5 smit:SMT_1558|3end_hypothetical_protein_1543855..1544005_NEGATIVE 415 130 83 53 5 smit:SMT_1155|3end_ABC-type_oligopeptide_transport_system,_periplasmic_component_1099183..1099333_NEGATIVE 408 105 84 22 1 smit:SMT_463|3end_para-aminobenzoate_synthetase_component_I_/_4-amino-4-deoxychorismate_lyase_402839..402989_POSITIVE 406 69 28 111 56 smit:SMT_1786|3end_phenylalanyl-tRNA_synthetase_beta_chain_1763235..1763385_NEGATIVE 388 130 83 53 5 smit:SMT_2006|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1926803..1926953_POSITIVE 369 137 119 19 1 smit:SMT_780|3end_peptidase_family_U32_688752..688902_NEGATIVE 365 64 21 62 5 smit:SMT_1750|3end_anthranilate_phosphoribosyltransferase_1729095..1729245_POSITIVE 364 130 88 63 27 smit:SMT_1985|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1914407..1914557_NEGATIVE 356 208 163 124 78 smit:SMT_1255|3end_cell_division_protein_FtsH_1220987..1221137_NEGATIVE 349 130 87 57 10 smit:SMT_1181|3end_phosphotransferase_system,_mannose/fructose-specific_component_IIA_1139413..1139563_NEGATIVE