Origin IGS: aagaagcaagtcctgaaatcgtagaagtaggtactggctttccagcacactggggtcatagctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacattcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgaggacccggctaggctggcggggttgccttactggttagcagaatgaatcaccgatacgcgagcgaacgtgaagcgactgctgctgcaaaacgtctgcgacctgagcaacaacatgaatggtcttcggtttccgtgtttcgtaagtctggaaacgcggaagtcagcgccctgcaccattatgttccggatctgcatcgcaggatgctgctggctaccctgtggacacctacatctgtattaacgaagcgctggnnnnnttaattaattaannnnnggctgttctcagggacaatttcacatctggagtatgaactcagaaaacccataaatccat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atggatttatgggttttctgagttcatactccagatgtgaaattgtccctgagaacagccnnnnnttaattaattaannnnnccagcgcttcgttaatacagatgtaggtgtccacagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagacttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggccaggacccaacgctgcccgaatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagctatgaccccagtgtgctggaaagccagtacctacttctacgatttcaggacttgcttctt Mask Tandem Repeat Region ================================================ aagaagcaagtcctgaaatcgtagaagtaggtactggctttccagcacactggggtcatagctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacattcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgaggacccggctaggctggcggggttgccttactggttagcagaatgaatcaccgatacgcgagcgaacgtgaagcgactgctgctgcaaaacgtctgcgacctgagcaacaacatgaatggtcttcggtttccgtgtttcgtaagtctggaaacgcggaagtcagcgccctgcaccattatgttccggatctgcatcgcaggatgctgctggctaccctgtggacacctacatctgtattaacgaagcgctggnnnnnttaattaattaannnnnggctgttctcagggacaatttcacatctggagtatgaactcagaaaacccataaatccat Find is-nt database================================================ Query_seq: SMT_1895:SMT_1896|SMT_1895:SMT_1896:hypothetical protein predicted by Glimmer/Critica:lysine decarboxylase:-><-:1847463..1848162 700 aagaagcaagtcctgaaatcgtagaagtaggtactggctttccagcacactggggtcatagctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacattcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgaggacccggctaggctggcggggttgccttactggttagcagaatgaatcaccgatacgcgagcgaacgtgaagcgactgctgctgcaaaacgtctgcgacctgagcaacaacatgaatggtcttcggtttccgtgtttcgtaagtctggaaacgcggaagtcagcgccctgcaccattatgttccggatctgcatcgcaggatgctgctggctaccctgtggacacctacatctgtattaacgaagcgctggnnnnnttaattaattaannnnnggctgttctcagggacaatttcacatctggagtatgaactcagaaaacccataaatccat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1895:SMT_1896|SMT_1895:SMT_1896:hypothetical protein predicted by Glimmer/Critica:lysine decarboxylase:-><-:1847463..1848162 700 aagaagcaagtcctgaaatcgtagaagtaggtactggctttccagcacactggggtcatagctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacattcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgaggacccggctaggctggcggggttgccttactggttagcagaatgaatcaccgatacgcgagcgaacgtgaagcgactgctgctgcaaaacgtctgcgacctgagcaacaacatgaatggtcttcggtttccgtgtttcgtaagtctggaaacgcggaagtcagcgccctgcaccattatgttccggatctgcatcgcaggatgctgctggctaccctgtggacacctacatctgtattaacgaagcgctggnnnnnttaattaattaannnnnggctgttctcagggacaatttcacatctggagtatgaactcagaaaacccataaatccat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1895:SMT_1896|SMT_1895:SMT_1896:hypothetical protein predicted by Glimmer/Critica:lysine decarboxylase:-><-:1847463..1848162 700 aagaagcaagtcctgaaatcgtagaagtaggtactggctttccagcacactggggtcatagctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacattcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgaggacccggctaggctggcggggttgccttactggttagcagaatgaatcaccgatacgcgagcgaacgtgaagcgactgctgctgcaaaacgtctgcgacctgagcaacaacatgaatggtcttcggtttccgtgtttcgtaagtctggaaacgcggaagtcagcgccctgcaccattatgttccggatctgcatcgcaggatgctgctggctaccctgtggacacctacatctgtattaacgaagcgctggnnnnnttaattaattaannnnnggctgttctcagggacaatttcacatctggagtatgaactcagaaaacccataaatccat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 7 Min: 134 Max: 617 Len: 484 Subject: UniRef90_P03850 Cluster: Hypothetical 10.0 kDa protein; n=1; Escherichia coli|Rep: Hypothetical 10.0 kDa protein - Escherichia coli HSP 1 e-value: 9.0E-20 bit: 61.6 Len: 87 Query Start:502 Query End:588 Subject Strand: null Subject Start: 24 Subject End: 52 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... F R K S G N A E V S A L H H Y V P D L H R R M L L A T L W ................................................................................................................ ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... F V K S G N A E V S A L H H Y V P D L H R R M L L A T L W ................................................................................................................ HSP 2 e-value: 9.0E-20 bit: 55.1 Len: 75 Query Start:434 Query End:508 Subject Strand: null Subject Start: 1 Subject End: 25 ................................................................................................................................................................................................................................................................................................................................................................................................................................................. V K R L L L Q N V C D L S N N M N G L R F P C F V ................................................................................................................................................................................................ ................................................................................................................................................................................................................................................................................................................................................................................................................................................. M K R L L L Q N V C D L S N N M N G L R F P C F V ................................................................................................................................................................................................ HSP 3 e-value: 9.0E-20 bit: 23.5 Len: 30 Query Start:588 Query End:617 Subject Strand: null Subject Start: 53 Subject End: 62 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... D T Y I C I N E A L ................................................................................... ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... N T Y I C I N E A L ................................................................................... Subject: UniRef90_P03852 Cluster: Hypothetical 15.3 kDa protein; n=6; Bacteria|Rep: Hypothetical 15.3 kDa protein - Escherichia coli HSP 1 e-value: 6.0E-17 bit: 58.2 Len: 105 Query Start:484 Query End:588 Subject Strand: null Subject Start: 71 Subject End: 103 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... P Q G S Q Q H P A M Q I R N I M V Q G A D F R V S R L T K H G N R R P ................................................................................................................ ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... P Q G S Q Q H P A M Q I R N I M V Q G A D F R V S R L - - Y E T R K P ................................................................................................................ HSP 2 e-value: 6.0E-17 bit: 48.1 Len: 105 Query Start:404 Query End:508 Subject Strand: null Subject Start: 98 Subject End: 132 ................................................................................................................................................................................................................................................................................................................................................................................................................... Y E T R K P K T I H X X X X X X X X X X X Q S L H V R S R I G D S F C ................................................................................................................................................................................................ ................................................................................................................................................................................................................................................................................................................................................................................................................... Y E T R K P K T I H V V A Q V A D V L Q Q Q S L H V R S R I G D S F C ................................................................................................................................................................................................ HSP 3 e-value: 6.0E-17 bit: 24.3 Len: 30 Query Start:588 Query End:617 Subject Strand: null Subject Start: 61 Subject End: 70 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Q R F V N T D V G V ................................................................................... ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Q R F V N T D V G V ................................................................................... Subject: UniRef90_A0ZNE4 Cluster: Molybdenum cofactor biosynthesis protein C; n=2; Nodularia spumigena CCY9414|Rep: Molybdenum cofactor biosynthesis protein C - Nodularia spumigena CCY9414 HSP 1 e-value: 5.0E-13 bit: 77.0 Len: 180 Query Start:409 Query End:588 Subject Strand: null Subject Start: 1 Subject End: 60 ........................................................................................................................................................................................................................................................................................................................................................................................................................ M N H R Y A S E R E A T A A A K R L R P E Q Q H E W S S V S V F R K S G N A E V S A L H H Y V P D L H R R M L L A T L W ................................................................................................................ ........................................................................................................................................................................................................................................................................................................................................................................................................................ M N H R Y A S E R E A T A A A K R L R P E Q Q H E W S S V S V F R K V W K R G S Q R P A P L C S G S A S Q M L L A T L W ................................................................................................................ Subject: UniRef90_UPI00005EEC00 Cluster: hypothetical protein EcolB7_01001251; n=1; Escherichia coli B7A|Rep: hypothetical protein EcolB7_01001251 - Escherichia coli B7A HSP 1 e-value: 1.0E-11 bit: 72.4 Len: 102 Query Start:409 Query End:510 Subject Strand: null Subject Start: 1 Subject End: 34 ........................................................................................................................................................................................................................................................................................................................................................................................................................ M N H R Y A S E R E A T A A A K R L R P E Q Q H E W S S V S V F R K .............................................................................................................................................................................................. ........................................................................................................................................................................................................................................................................................................................................................................................................................ M N H R Y A S E R E A T A A A K R L R P E Q Q H E W S S V S V F R K .............................................................................................................................................................................................. Subject: UniRef90_Q19NK3 Cluster: Hypothetical protein; n=2; root|Rep: Hypothetical protein - Escherichia coli O1:K1 / APEC HSP 1 e-value: 4.0E-10 bit: 67.4 Len: 105 Query Start:297 Query End:401 Subject Strand: null Subject Start: 78 Subject End: 112 ........................................................................................................................................................................................................................................................................................................ R R K E H S G S V G S W P R V R M I V L L S L R T R L G W R G C L T G ........................................................................................................................................................................................................................................................................................................... ........................................................................................................................................................................................................................................................................................................ R T R R I S G S V G S W P R V R M I V L L S L R T R L G W R G C L T G ........................................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q19NK4 Cluster: Hypothetical protein; n=1; Escherichia coli APEC O1|Rep: Hypothetical protein - Escherichia coli O1:K1 / APEC HSP 1 e-value: 8.0E-7 bit: 52.0 Len: 81 Query Start:508 Query End:588 Subject Strand: null Subject Start: 127 Subject End: 153 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... P Q G S Q Q H P A M Q I R N I M V Q G A D F R V S R L ................................................................................................................ ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... P Q G S Q Q H P A M Q I R N I M V Q G A D F R C Q Q I ................................................................................................................ HSP 2 e-value: 8.0E-7 bit: 24.3 Len: 30 Query Start:588 Query End:617 Subject Strand: null Subject Start: 117 Subject End: 126 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Q R F V N T D V G V ................................................................................... ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Q R F V N T D V G V ................................................................................... Subject: UniRef90_Q9KHC4 Cluster: SocE; n=1; Myxococcus xanthus|Rep: SocE - Myxococcus xanthus HSP 1 e-value: 1.0E-6 bit: 55.8 Len: 168 Query Start:134 Query End:301 Subject Strand: null Subject Start: 266 Subject End: 322 ..................................................................................................................................... C I N E S A N A R G E A V C V L G A L P L P R S L T R C A R S F G C G E R Y Q L - T Q R R * Y G Y P Q N Q G I T Q ............................................................................................................................................................................................................................................................................................................................................................................................................... ..................................................................................................................................... C I R D P A T A R S E A V W V L V A L P L L R S R T R C V R S V G C G G A V S A H S P G R P Y G D P Q P Q G M A Q ............................................................................................................................................................................................................................................................................................................................................................................................................... aagaagcaagtcctgaaatcgtagaagtagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncctgcaggcatgcaagctcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngnnnnnttaattaattaannnnnggctgttctcagggacaatttcacatctggagtatgaactcagaaaacccataaatccat Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: + Start: 78 End: 206 ............................................................................. M T I L A Q G D P L E S T C R H A S S A L M N R P T R G E R R F A Y W A L F R F L A H .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 62 End: 265 ............................................................. M F P D L G D H P S T G G S S R V D L Q A C K L C I N E S A N A R G E A V C V L G A L P L P R S L T R C A R S F G C G E ................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 145 End: 345 ................................................................................................................................................ M G Q R A G R G G L R I G R S S A S S L T D S L R S V V R L R R A V S A H S K A V I R L S T E S G D N A G K N I R A A L ................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 39 Strand: + Start: 293 End: 409 .................................................................................................................................................................................................................................................................................................... M T Q E R T F G Q R W V L A T G A H D R A P V V E D P A R L A G L P Y W L A E ................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 413 End: 604 ............................................................................................................................................................................................................................................................................................................................................................................................................................ M T D T R A N V K R L L L Q N V C D L S N N M N G L R F P C F V S L E T R K S A P C T I M F R I C I A G C C W L P C G H ................................................................................................ Protein_Len: 60 Strand: + Start: 397 End: 624 ............................................................................................................................................................................................................................................................................................................................................................................................................ M V S R M N H R Y A S E R E A T A A A K R L R P E Q Q H E W S S V S V F R K S G N A E V S A L H H Y V P D L H R R M L L ............................................................................ Protein_Len: 38 Strand: - Start: 417 End: 530 ................................................................................................................................................................................................................................................................................................................................................................................................................................ R Y A L S R S A V A A A F R R R G S C C C S H D E T E T N R L D P F A S T M .......................................................................................................................................................................... Protein_Len: 55 Strand: - Start: 397 End: 561 ............................................................................................................................................................................................................................................................................................................................................................................................................ Q N A S H I V S V R A F T F R S S S C F T Q S R L L L M F P R R N G H K T L R S V R F D A G Q V M I N R I Q M ........................................................................................................................................... Protein_Len: 48 Strand: - Start: 206 End: 349 ............................................................................................................................................................................................................. Q S A A S P R E A A A L P I L E S L P P L V T I W L I L P Y R L F S C E P L T P D Q G R T R M M ............................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 51 Strand: - Start: 1 End: 153 L L L D Q F R L L L Y Q S E L V S P D Y S N G S R P S W G L V P P D E L T S R C A H L S Q M L S D A M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... aagaagcaagtcctgaaatcgtagaagtagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncctgcaggcatgcaagctcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngnnnnnttaattaattaannnnnggctgttctcagggacaatttcacatctggagtatgaactcagaaaacccataaatccat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aagaagcaagtcctgaaatcgtagaagtagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncctgcaggcatgcaagctcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngnnnnnttaattaattaannnnnggctgttctcagggacaatttcacatctggagtatgaactcagaaaacccataaatccat Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 42 HP_score: -3.7 Tail_Score: -2.70932 Start: 336 End: 359 Full_Region: GTTGGGTCCTGGCCA CGGGTGCGC ATGATC GTGCTCCTG TCGTTGAGGACCCGG ...............................................................................................................................................................................................................................................................................................................................................CGGGTGCGCATGATCGTGCTCCTG..................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 42 HP_score: -2.7 Tail_Score: -2.90075 Start: 338 End: 357 Full_Region: TGGGTCCTGGCCACG GGTGCGC ATGATC GTGCTCC TGTCGTTGAGGACCC .................................................................................................................................................................................................................................................................................................................................................GGTGCGCATGATCGTGCTCC....................................................................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: SMT_1895:SMT_1896|SMT_1895:SMT_1896:hypothetical protein predicted by Glimmer/Critica:lysine decarboxylase:-><-:1847463..1848162 700 aagaagcaagtcctgaaatcgtagaagtagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncctgcaggcatgcaagctcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngnnnnnttaattaattaannnnnggctgttctcagggacaatttcacatctggagtatgaactcagaaaacccataaatccat Intra-Species Hit: Count: 1 Min: 1 Max: 700 Len: 700 Subject: smit_SMT_1895_SMT_1896|hypothetical protein predicted by Glimmer/Critica:lysine decarboxylase|POSITIVE:NEGATIVE|[1847463,1848162]|700 HSP 1 e-value: 2.0E-24 bit: 115.0 Len: 58 Query Start:643 Query End:700 Subject Strand: POSITIVE Subject Start: 643 Subject End: 700 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ctgttctcagggacaatttcacatctggagtatgaactcagaaaacccataaatccat ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ctgttctcagggacaatttcacatctggagtatgaactcagaaaacccataaatccat HSP 2 e-value: 4.0E-7 bit: 58.0 Len: 29 Query Start:1 Query End:29 Subject Strand: POSITIVE Subject Start: 1 Subject End: 29 aagaagcaagtcctgaaatcgtagaagta............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... aagaagcaagtcctgaaatcgtagaagta............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGAAGCAAGUCCUGAAAUCGUAGAAGUAGGUACUGGCUUUCCAGCACACUGGGGUCAUAGCUGUUUCCUGAUCUAGGUGACCAUCCUAGCACAGGGGGAUCCUCUAGAGUCGACCUGCAGGCAUGCAAGCUCUGCAUUAAUGAAUCGGCCAACGCGCGGGGAGAGGCGGUUUGCGUAUUGGGCGCUCUUCCGCUUCCUCGCUCACUGACUCGCUGCGCUCGGUCGUUCGGCUGCGGCGAGCGGUAUCAGCUCACUCAAAGGCGGUAAUACGGUUAUCCACAGAAUCAGGGGAUAACGCAGGAAAGAACAUUCGGGCAGCGUUGGGUCCUGGCCACGGGUGCGCAUGAUCGUGCUCCUGUCGUUGAGGACCCGGCUAGGCUGGCGGGGUUGCCUUACUGGUUAGCAGAAUGAAUCACCGAUACGCGAGCGAACGUGAAGCGACUGCUGCUGCAAAACGUCUGCGACCUGAGCAACAACAUGAAUGGUCUUCGGUUUCCGUGUUUCGUAAGUCUGGAAACGCGGAAGUCAGCGCCCUGCACCAUUAUGUUCCGGAUCUGCAUCGCAGGAUGCUGCUGGCUACCCUGUGGACACCUACAUCUGUAUUAACGAAGCGCUGGNNNNNUUAAUUAAUUAANNNNNGGCUGUUCUCAGGGACAAUUUCACAUCUGGAGUAUGAACUCAGAAAACCCAUAAAUCCAU ..((((....((((((..(((((((.((((((.((((....))))..(((.(((((..((((.((.(((((..(((((.......))))).....(.((((((....((((((...(((((((......)).))))).................(.(((((((..(((((...((((.....))))....)))))))))))).)..)))))).....((((((((((.((..((((((.((((((....((((((.......((((......(((((((.(........))))))))...........(((((..((...(((((((((((((.(((((.((((......)))).))))).))).))))))))))..(((..(..(((...)))..)..))).)).)))))....))))...((....))...))).))).))))))))))))...))...)))))).))))...(((((((.(((.....(..(((((((((((.........)))))))))))..).((.....)).))))))))))..)))))).)....))))).)).))))...))))))))...)))))).)))......))))...(((.......((((....)))).....))).....))))))....))))...((((.(((....)))))))................ (-252.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUGGAUUUAUGGGUUUUCUGAGUUCAUACUCCAGAUGUGAAAUUGUCCCUGAGAACAGCCNNNNNUUAAUUAAUUAANNNNNCCAGCGCUUCGUUAAUACAGAUGUAGGUGUCCACAGGGUAGCCAGCAGCAUCCUGCGAUGCAGAUCCGGAACAUAAUGGUGCAGGGCGCUGACUUCCGCGUUUCCAGACUUACGAAACACGGAAACCGAAGACCAUUCAUGUUGUUGCUCAGGUCGCAGACGUUUUGCAGCAGCAGUCGCUUCACGUUCGCUCGCGUAUCGGUGAUUCAUUCUGCUAACCAGUAAGGCAACCCCGCCAGCCUAGCCGGGUCCUCAACGACAGGAGCACGAUCAUGCGCACCCGUGGCCAGGACCCAACGCUGCCCGAAUGUUCUUUCCUGCGUUAUCCCCUGAUUCUGUGGAUAACCGUAUUACCGCCUUUGAGUGAGCUGAUACCGCUCGCCGCAGCCGAACGACCGAGCGCAGCGAGUCAGUGAGCGAGGAAGCGGAAGAGCGCCCAAUACGCAAACCGCCUCUCCCCGCGCGUUGGCCGAUUCAUUAAUGCAGAGCUUGCAUGCCUGCAGGUCGACUCUAGAGGAUCCCCCUGUGCUAGGAUGGUCACCUAGAUCAGGAAACAGCUAUGACCCCAGUGUGCUGGAAAGCCAGUACCUACUUCUACGAUUUCAGGACUUGCUUCUU .((((..((((..(((...)))..)))).)))).....(((...((((.(((((.(............................(((..............(((.((((((.((((((((..((..((((....))))...)).(((((...........((((.(((((((..(((((((((((((...............))))))......((.....(((((((((..(.(((...))).)...))))))))).(((((.(((.((((((.((((.(((((..(((...((((........(((((.....((.((((......(((((((...(.((.((.((.(......).)).)).)).)..)))))))...))))..))............((((((((((.(.......).)))))).)))).....)))))...((((((.......)))))).))))..)))..)))))))))))))))...))))))))))..)))))))))))))).....((.....))........))))(((((((..........(((((.((......))))))))))))))......))))).))))))........)).)))))).))).(....).)))........((.(((((((....)))))))))........).)))))))))....))).. (-229.26) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1211 239 191 177 129 smit:SMT_2005|5end_pullulanase,_type_I_1926289..1926519_NEGATIVE 1168 200 151 206 157 smit:SMT_304|5end_hypothetical_protein_246580..246810_POSITIVE 1142 199 151 231 183 smit:SMT_1967|5end_dicarboxylate/amino_acid:cation_(Na+_or_H+)_symporter_(DAACS)_family_protein_1899683..1899913_NEGATIVE 1142 199 151 231 183 smit:SMT_1690|5end_K06148_ATP-binding_cassette,_subfamily_C,_bacterial_1674779..1675009_NEGATIVE 1142 199 151 231 183 smit:SMT_779|5end_ribonuclease_BN,_putative_688530..688760_NEGATIVE 1132 170 121 202 153 smit:SMT_388|5end_branched-chain_amino_acid_transport_system_II_carrier_protein_321172..321402_POSITIVE 1103 170 121 202 153 smit:SMT_1955|5end_carbamoyl-phosphate_synthase_small_chain_1889408..1889638_NEGATIVE 1103 170 121 123 74 smit:SMT_464|5end_ribonuclease_Z_402867..403097_POSITIVE 1097 130 81 226 177 smit:SMT_2016|5end_tyrosine_recombinase_1933425..1933655_NEGATIVE 1097 130 81 199 150 smit:SMT_2008|5end_K07485_transposase_1928662..1928892_NEGATIVE 1097 130 81 217 168 smit:SMT_2001|5end_membrane_protease_protein_family_1923387..1923617_POSITIVE 1097 130 81 162 113 smit:SMT_1961|5end_K07485_transposase_1894206..1894436_POSITIVE 1097 130 81 206 157 smit:SMT_1954|5end_6-phospho-beta-glucosidase_1888548..1888778_POSITIVE 1097 130 81 197 148 smit:SMT_1944|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1879494..1879724_NEGATIVE 1097 130 81 211 162 smit:SMT_1155|5end_ABC-type_oligopeptide_transport_system,_periplasmic_component_1099665..1099895_NEGATIVE 1071 120 71 231 182 smit:SMT_2044|5end_transcriptional_activator_TipA,_putative_1955885..1956115_NEGATIVE 1071 120 71 228 179 smit:SMT_1686|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1670175..1670405_POSITIVE 1049 100 51 211 162 smit:SMT_2006|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1926472..1926702_POSITIVE 874 80 35 188 143 smit:SMT_621|5end_K03346_replication_initiation_and_membrane_attachment_protein_528794..529024_POSITIVE 874 80 35 112 67 smit:SMT_620|5end_hypothetical_protein_predicted_by_Glimmer/Critica_528718..528948_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1227 240 191 50 1 smit:SMT_780|3end_peptidase_family_U32_688752..688902_NEGATIVE 1181 360 311 66 17 smit:SMT_478|3end_hypothetical_protein_predicted_by_Glimmer/Critica_415257..415407_POSITIVE 1132 170 121 68 19 smit:SMT_387|3end_hypothetical_protein_predicted_by_Glimmer/Critica_321118..321268_POSITIVE 1103 170 121 73 24 smit:SMT_1956|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1889537..1889687_NEGATIVE 1034 369 321 53 5 smit:SMT_2025|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1940995..1941145_NEGATIVE 977 500 451 55 6 smit:SMT_1953|3end_hypothetical_protein_1888107..1888257_POSITIVE 951 339 291 67 19 smit:SMT_1874|3end_hypothetical_protein_1832623..1832773_NEGATIVE 879 400 354 65 20 smit:SMT_1559|3end_6-phosphofructokinase_1544614..1544764_NEGATIVE 856 689 641 80 31 smit:SMT_2037|3end_lysine_decarboxylase_1947880..1948030_NEGATIVE 856 689 641 80 31 smit:SMT_1418|3end_lysine_decarboxylase_1401687..1401837_NEGATIVE 627 80 48 33 1 smit:SMT_619|3end_riboflavin_kinase_/_FMN_adenylyltransferase_528719..528869_POSITIVE 508 262 237 26 1 smit:SMT_1968|3end_transcription-repair_coupling_factor_1899951..1900101_NEGATIVE 473 618 592 27 1 smit:SMT_1884|3end_hypothetical_protein_1839238..1839388_POSITIVE 457 379 332 71 21 smit:SMT_904|3end_hypothetical_protein_852294..852444_NEGATIVE 430 219 174 55 2 smit:SMT_1750|3end_anthranilate_phosphoribosyltransferase_1729095..1729245_POSITIVE 423 238 193 84 25 smit:SMT_1941|3end_hypothetical_protein_1877663..1877813_POSITIVE 421 380 331 66 5 smit:SMT_2075|3end_phosphoribosylamine--glycine_ligase_1977769..1977919_NEGATIVE 408 177 156 22 1 smit:SMT_463|3end_para-aminobenzoate_synthetase_component_I_/_4-amino-4-deoxychorismate_lyase_402839..402989_POSITIVE 406 229 181 132 76 smit:SMT_977|3end_conserved_hypothetical_protein,_Rrf2_family_protein_925252..925402_NEGATIVE 397 140 100 110 56 smit:SMT_1786|3end_phenylalanyl-tRNA_synthetase_beta_chain_1763235..1763385_NEGATIVE