Origin IGS: tcctctccacctttccagcacactggactggccgtcgttttacaaggtctagtccgacctagactcaggggtaccgagctcgaattctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagcccccgacacccgccaaacacccgctgacgcgcccctgacgggcttgtctgctccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaagtttcaccgtcatcaccgaaacgcgcgagacgaaagggctcgtgatacgcctattttataggttatgtcatgannnnnttaattaattaannnnngggtcctcaacgacaggagcacgatcatgcgcacccgtgggccaggacccaacgctgcccgaatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagctatgaccccagtgtgctggaaagagttgctctatatcctgaaaactatttmgcaatttatgatttaccattggataccgatctcaatgaattactacactatccatctgtgtttattgggaaagaggagtttgagtagaaatcttgctttacctagatagcttattcccagcagtttaagaagaaaggaaaaattaacaac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gttgttaatttttcctttcttcttaaactgctgggaataagctatctaggtaaagcaagatttctactcaaactcctctttcccaataaacacagatggatagtgtagtaattcattgagatcggtatccaatggtaaatcataaattgckaaatagttttcaggatatagagcaactctttccagcacactggggtcatagctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacattcgggcagcgttgggtcctggcccacgggtgcgcatgatcgtgctcctgtcgttgaggacccnnnnnttaattaattaannnnntcatgacataacctataaaataggcgtatcacgagccctttcgtctcgcgcgtttcggtgatgacggtgaaacttctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccggagcagacaagcccgtcaggggcgcgtcagcgggtgtttggcgggtgtcgggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagaattcgagctcggtacccctgagtctaggtcggactagaccttgtaaaacgacggccagtccagtgtgctggaaaggtggagagga Mask Tandem Repeat Region ================================================ tcctctccacctttccagcacactggactggccgtcgttttacaaggtctagtccgacctagactcaggggtaccgagctcgaattctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagcccccgacacccgccaaacacccgctgacgcgcccctgacgggcttgtctgctccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaagtttcaccgtcatcaccgaaacgcgcgagacgaaagggctcgtgatacgcctattttataggttatgtcatgannnnnttaattaattaannnnngggtcctcaacgacaggagcacgatcatgcgcacccgtgggccaggacccaacgctgcccgaatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagctatgaccccagtgtgctggaaagagttgctctatatcctgaaaactatttmgcaatttatgatttaccattggataccgatctcaatgaattactacactatccatctgtgtttattgggaaagaggagtttgagtagaaatcttgctttacctagatagcttattcccagcagtttaagaagaaaggaaaaattaacaac Find is-nt database================================================ Query_seq: SMT_1885:SMT_1886|SMT_1885:SMT_1886:hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica:<-->:1840148..1841123 976 tcctctccacctttccagcacactggactggccgtcgttttacaaggtctagtccgacctagactcaggggtaccgagctcgaattctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagcccccgacacccgccaaacacccgctgacgcgcccctgacgggcttgtctgctccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaagtttcaccgtcatcaccgaaacgcgcgagacgaaagggctcgtgatacgcctattttataggttatgtcatgannnnnttaattaattaannnnngggtcctcaacgacaggagcacgatcatgcgcacccgtgggccaggacccaacgctgcccgaatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagctatgaccccagtgtgctggaaagagttgctctatatcctgaaaactatttmgcaatttatgatttaccattggataccgatctcaatgaattactacactatccatctgtgtttattgggaaagaggagtttgagtagaaatcttgctttacctagatagcttattcccagcagtttaagaagaaaggaaaaattaacaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1885:SMT_1886|SMT_1885:SMT_1886:hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica:<-->:1840148..1841123 976 tcctctccacctttccagcacactggactggccgtcgttttacaaggtctagtccgacctagactcaggggtaccgagctcgaattctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagcccccgacacccgccaaacacccgctgacgcgcccctgacgggcttgtctgctccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaagtttcaccgtcatcaccgaaacgcgcgagacgaaagggctcgtgatacgcctattttataggttatgtcatgannnnnttaattaattaannnnngggtcctcaacgacaggagcacgatcatgcgcacccgtgggccaggacccaacgctgcccgaatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagctatgaccccagtgtgctggaaagagttgctctatatcctgaaaactatttmgcaatttatgatttaccattggataccgatctcaatgaattactacactatccatctgtgtttattgggaaagaggagtttgagtagaaatcttgctttacctagatagcttattcccagcagtttaagaagaaaggaaaaattaacaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1885:SMT_1886|SMT_1885:SMT_1886:hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica:<-->:1840148..1841123 976 tcctctccacctttccagcacactggactggccgtcgttttacaaggtctagtccgacctagactcaggggtaccgagctcgaattctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagcccccgacacccgccaaacacccgctgacgcgcccctgacgggcttgtctgctccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaagtttcaccgtcatcaccgaaacgcgcgagacgaaagggctcgtgatacgcctattttataggttatgtcatgannnnnttaattaattaannnnngggtcctcaacgacaggagcacgatcatgcgcacccgtgggccaggacccaacgctgcccgaatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagctatgaccccagtgtgctggaaagagttgctctatatcctgaaaactatttmgcaatttatgatttaccattggataccgatctcaatgaattactacactatccatctgtgtttattgggaaagaggagtttgagtagaaatcttgctttacctagatagcttattcccagcagtttaagaagaaaggaaaaattaacaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 9 Min: 4 Max: 910 Len: 907 Subject: UniRef90_A0ZLG1 Cluster: Beta-D-galactosidase; n=1; Nodularia spumigena CCY9414|Rep: Beta-D-galactosidase - Nodularia spumigena CCY9414 HSP 1 e-value: 3.0E-23 bit: 112.0 Len: 162 Query Start:4 Query End:165 Subject Strand: null Subject Start: 19 Subject End: 72 ... S P P F Q H T G L A V V L Q G L V R P R L R G T E L E F W R N S E E A R T D R P S Q Q L R S L N G E W R L M ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ... S P P F Q H T G L A V V L Q G L V R P R L T G T E L E F W R N S E E A R T D R P S Q Q L R S L N G E W R L M ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q6QI34 Cluster: LRRGT00174; n=1; Rattus norvegicus|Rep: LRRGT00174 - Rattus norvegicus (Rat) HSP 1 e-value: 6.0E-17 bit: 90.9 Len: 240 Query Start:73 Query End:312 Subject Strand: null Subject Start: 275 Subject End: 353 ........................................................................ E Q T S P S G A R Q R V F G G C R - - G L A * L C G I R A D C T E S A P Y A V * N T A Q M R K E K I P H Q A P F A I Q A A Q L L G R A I G A G L F A I T P E F E L G ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ ........................................................................ E Q T S P S G - - - R V I G C W R V S G L A Y T M R H Q S R L Y E S A P Y A V C N T A Q M R K E K I T H Q A P F A I Q D A H M L G R A I G A G L F A I T P A G E R G ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Subject: UniRef90_UPI0000ECAFAB Cluster: UPI0000ECAFAB related cluster; n=1; Gallus gallus|Rep: UPI0000ECAFAB UniRef100 entry - Gallus gallus HSP 1 e-value: 3.0E-11 bit: 55.5 Len: 81 Query Start:144 Query End:224 Subject Strand: null Subject Start: 50 Subject End: 76 ............................................................................................................................................... E W R M A P D A V F S P Y A S V R Y F T P H M V H S Q ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ ............................................................................................................................................... E W R M A P D A V F S P Y A S V R Y F T P H M G S N Q ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ HSP 2 e-value: 3.0E-11 bit: 34.7 Len: 63 Query Start:100 Query End:162 Subject Strand: null Subject Start: 36 Subject End: 53 ................................................................................................... E E A R T D R P S Q Q L R S L N G E W R L .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. ................................................................................................... E E A R T D R P S Q Q L R S - - - E W R M .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. HSP 3 e-value: 3.0E-11 bit: 21.9 Len: 30 Query Start:20 Query End:49 Subject Strand: null Subject Start: 1 Subject End: 10 ................... T L D W P S F Y K V ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ................... T I H W P S F Y N V ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q03MY9 Cluster: Uncharacterized conserved protein; n=3; Streptococcus thermophilus|Rep: Uncharacterized conserved protein - Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9) HSP 1 e-value: 3.0E-10 bit: 68.9 Len: 117 Query Start:794 Query End:910 Subject Strand: null Subject Start: 16 Subject End: 54 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... E R V A L Y P E N Y X A I Y D L P L D T D L N E L L H Y P S V F I G K E E F E .................................................................. ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... E L I A Q Y P E N Y L A I Y D L P M D T D L N T L N H Y P S V W I G K E E F E .................................................................. Subject: UniRef90_Q97T94 Cluster: Hypothetical protein; n=3; Streptococcus pneumoniae|Rep: Hypothetical protein - Streptococcus pneumoniae HSP 1 e-value: 1.0E-9 bit: 67.0 Len: 117 Query Start:794 Query End:910 Subject Strand: null Subject Start: 37 Subject End: 75 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... E R V A L Y P E N Y X A I Y D L P L D T D L N E L L H Y P S V F I G K E E F E .................................................................. ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... E L A A Q Y P E N Y L A I Y N V P L D T D L N T L D H Y P S V F I G K E E F E .................................................................. Subject: UniRef90_O21999 Cluster: LacZ-alpha; n=1; Cloning vector pAL-Z|Rep: LacZ-alpha - Cloning vector pAL-Z HSP 1 e-value: 2.0E-7 bit: 59.3 Len: 105 Query Start:88 Query End:192 Subject Strand: null Subject Start: 70 Subject End: 106 ....................................................................................... W R N S E E A R T D R P S Q Q L R S L N G E W R - - L M R Y F L L T H L C ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ ....................................................................................... W R N S E E A R T D R P S Q Q L R S L N G E W R D K L A L Y A L M Q F L C ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Subject: UniRef90_A3CK04 Cluster: Hypothetical protein; n=1; Streptococcus sanguinis SK36|Rep: Hypothetical protein - Streptococcus sanguinis (strain SK36) HSP 1 e-value: 3.0E-7 bit: 58.5 Len: 117 Query Start:794 Query End:910 Subject Strand: null Subject Start: 37 Subject End: 75 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... E R V A L Y P E N Y X A I Y D L P L D T D L N E L L H Y P S V F I G K E E F E .................................................................. ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... E L V A Q H P E N Y L A I Y D L P L D K D L N T L D H Y P S V V V V K E E L E .................................................................. Subject: UniRef90_Q9KHC4 Cluster: SocE; n=1; Myxococcus xanthus|Rep: SocE - Myxococcus xanthus HSP 1 e-value: 2.0E-6 bit: 55.8 Len: 168 Query Start:535 Query End:702 Subject Strand: null Subject Start: 266 Subject End: 322 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... C I N E S A N A R G E A V C V L G A L P L P R S L T R C A R S F G C G E R Y Q L - T Q R R * Y G Y P Q N Q G I T Q .................................................................................................................................................................................................................................................................................. ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... C I R D P A T A R S E A V W V L V A L P L L R S R T R C V R S V G C G G A V S A H S P G R P Y G D P Q P Q G M A Q .................................................................................................................................................................................................................................................................................. Subject: UniRef90_Q8GEG0 Cluster: Hypothetical protein; n=1; Erwinia amylovora|Rep: Hypothetical protein - Erwinia amylovora (Fire blight bacteria) HSP 1 e-value: 5.0E-6 bit: 54.7 Len: 135 Query Start:25 Query End:159 Subject Strand: null Subject Start: 67 Subject End: 120 ........................ G L A V V L Q - - - - - - - G L V R P R L R G T E L E F - - W R N S E E A R T D R P S Q Q L R S L N G E W R ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. ........................ G L A V V L Q R R D W E N P G V T Q L N R L A A H P P F A S W R N S E E A R T D R P S Q Q L R X L N G E W R ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. tccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaagtttcaccgtcatcaccgaaacgcgcgagacgaaagggctcgtgatacgcctattttataggttatgtcatgannnnnttaattaattaannnnngggtcctcaacgacaggagcacgatcatgcgcacccgtgggccaggacccaacgctgcccgaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngagcttgcatgcctgcaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntagaaatcttgctttacctagatagcttattcccagcagtttaagaagaaaggaaaaattaacaac Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 28 End: 246 ........................... M A V V L Q G L V R P R L R G T E L E F W R N S E E A R T D R P S Q Q L R S L N G E W R L M R Y F L L T H L C G I S H R .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: + Start: 317 End: 409 ............................................................................................................................................................................................................................................................................................................................ M R L Q T S C D R L R E L H V S E V S P S S P K R A R R K G S ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 246 End: 425 ..................................................................................................................................................................................................................................................... M V K P A P D T R Q T P A D A P L T G L S A P A S A Y R Q A V T V S G S C M C Q K F H R H H R N A R D E R A R D T P I L ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 43 Strand: + Start: 453 End: 581 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M X X X V L N D R S T I M R T R G P G P N A A R M F F P A L S P D S V D N R I T A F E ........................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 44 Strand: + Start: 499 End: 630 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M G Q D P T L P E C S F L R Y P L I L W I T V L P P L S E L I P L A A A E R P S A A S Q .......................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 683 End: 910 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M A D S L M Q S L H A C R S T L E D P P V L G W S P R S G N S Y D P S V L E R V A L Y P E N Y X A I Y D L P L D T D L N .................................................................. Protein_Len: 52 Strand: + Start: 777 End: 932 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M T P V C W K E L L Y I L K T I X Q F M I Y H W I P I S M N Y Y T I H L C L L G K R S L S R N L A L P R ............................................ Protein_Len: 43 Strand: - Start: 630 End: 758 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... H A L F R F L A W Y A F R R E G R T P R N M L A S S A H R C T S E L P D G Q A L I T M .......................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 571 End: 774 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... E G C G F S R A C R T L S R P L P L A G L V C V A E G R A N A S E N I C L K C A Q L D V R S S G G T S P H D G L D P F M .......................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 449 End: 691 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................ L A A R I N K G A N D G S E T S L R I V A K S H A S V A R R L R V V S R L S D T L S S A S S R G I R L G G R G A R Q G M ............................................................................................................................................................................................................................................................................................. Protein_Len: 45 Strand: - Start: 52 End: 186 ................................................... D S R S E P T G L E F E P T I A F L G A G I A R G L L Q A A Q I A F P A Q H P I K E K R M ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 3 End: 413 .. E S A A Y N L W G G V G A L C G S V R G Q R A Q R S R C G S V S L S H G D G P A A H T L L K V T M V S V R S V F P S T M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... tccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaagtttcaccgtcatcaccgaaacgcgcgagacgaaagggctcgtgatacgcctattttataggttatgtcatgannnnnttaattaattaannnnngggtcctcaacgacaggagcacgatcatgcgcacccgtgggccaggacccaacgctgcccgaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngagcttgcatgcctgcaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntagaaatcttgctttacctagatagcttattcccagcagtttaagaagaaaggaaaaattaacaac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaagtttcaccgtcatcaccgaaacgcgcgagacgaaagggctcgtgatacgcctattttataggttatgtcatgannnnnttaattaattaannnnngggtcctcaacgacaggagcacgatcatgcgcacccgtgggccaggacccaacgctgcccgaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngagcttgcatgcctgcaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntagaaatcttgctttacctagatagcttattcccagcagtttaagaagaaaggaaaaattaacaac Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1885:SMT_1886|SMT_1885:SMT_1886:hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica:<-->:1840148..1841123 976 tccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaagtttcaccgtcatcaccgaaacgcgcgagacgaaagggctcgtgatacgcctattttataggttatgtcatgannnnnttaattaattaannnnngggtcctcaacgacaggagcacgatcatgcgcacccgtgggccaggacccaacgctgcccgaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngagcttgcatgcctgcaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntagaaatcttgctttacctagatagcttattcccagcagtttaagaagaaaggaaaaattaacaac Intra-Species Hit: Count: 2 Min: 313 Max: 976 Len: 664 Subject: smit_SMT_1885_SMT_1886|hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1840148,1841123]|976 HSP 1 e-value: 2.0E-93 bit: 345.0 Len: 213 Query Start:313 Query End:525 Subject Strand: POSITIVE Subject Start: 313 Subject End: 525 ........................................................................................................................................................................................................................................................................................................................cggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaagtttcaccgtcatcaccgaaacgcgcgagacgaaagggctcgtgatacgcctattttataggttatgtcatgannnnnttaattaattaannnnnnnntcctcaacgacaggagcacgatcatgcgcacccgtgggccaggacccaacgctgcccgaa................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ........................................................................................................................................................................................................................................................................................................................cggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaagtttcaccgtcatcaccgaaacgcgcgagacgaaagggctcgtgatacgcctattttataggttatgtcatgannnnnttaattaattaannnnngggtcctcaacgacaggagcacgatcatgcgcacccgtgggccaggacccaacgctgcccgaa................................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 5.0E-29 bit: 131.0 Len: 66 Query Start:911 Query End:976 Subject Strand: POSITIVE Subject Start: 911 Subject End: 976 ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tagaaatcttgctttacctagatagcttattcccagcagtttaagaagaaaggaaaaattaacaac ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tagaaatcttgctttacctagatagcttattcccagcagtttaagaagaaaggaaaaattaacaac Subject: smit_SMT_1946_SMT_1947|sucrose-6-phosphate hydrolase:lysine decarboxylase|POSITIVE:NEGATIVE|[1881266,1882402]|1137 HSP 1 e-value: 1.0E-20 bit: 103.0 Len: 60 Query Start:466 Query End:525 Subject Strand: NEGATIVE Subject Start: 726 Subject End: 784 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tcctcaacgacaggagcacgatcatgcgcacccgtgggccaggacccaacgctgcccgaa................................................................................................................................................................................................................................................................................................................................................................................................................................................................... .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tcctcaacgacaggagcacgatcatgcgcacccgt-ggccaggacccaacgctgcccgaa................................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-19 bit: 99.6 Len: 50 Query Start:313 Query End:362 Subject Strand: NEGATIVE Subject Start: 10 Subject End: 59 ........................................................................................................................................................................................................................................................................................................................cggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgt...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ........................................................................................................................................................................................................................................................................................................................cggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgt...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGGCAUCCGCUUACAGACAAGCUGUGACCGUCUCCGGGAGCUGCAUGUGUCAGAAGUUUCACCGUCAUCACCGAAACGCGCGAGACGAAAGGGCUCGUGAUACGCCUAUUUUAUAGGUUAUGUCAUGANNNNNUUAAUUAAUUAANNNNNGGGUCCUCAACGACAGGAGCACGAUCAUGCGCACCCGUGGGCCAGGACCCAACGCUGCCCGAAUGUUCUUUCCUGCGUUAUCCCCUGAUUCUGUGGAUAACCGUAUUACCGCCUUUGAGUGAGCUGAUACCGCUCGCCGCAGCCGAACGACCGAGCGCAGCGAGUCAGUGAGCGAGGAAGCGGAAGAGCGCCCAAUACGCAAACCGCCUCUCCCCGCGCGUUGGCCGAUUCAUUAAUGCAGAGCUUGCAUGCCUGCAGGUCGACUCUAGAGGAUCCCCCUGUGCUAGGAUGGUCACCUAGAUCAGGAAACAGCUAUGACCCCAGUGUGCUGGAAAGAGUUGCUCUAUAUCCUGAAAACUAUUUMGCAAUUUAUGAUUUACCAUUGGAUACCGAUCUCAAUGAAUUACUACACUAUCCAUCUGUGUUUAUUGGGAAAGAGGAGUUUGAGUAGAAAUCUUGCUUUACCUAGAUAGCUUAUUCCCAGCAGUUUAAGAAGAAAGGAAAAAUUAACAAC (((.((((((.....((((.((.((..(((....)))..)).))...))))...............((((..((((((((.((((.(((.((((.(((((((.((((((...))))))..))))))).......................(((((((...(.((.((.((.(......).)).)).)).)...)))))))......)))).....))))))).)))))).))...))))...))))))..))).....((..((((...(((((((.....((((((.((.((((......)).)))).)))))).(((((((.(((....((((((((.........((.....))((((..(((((((((((............(((((.((......)))))))(((((..(((((..((((..((((...))))..))))..)))))...(....).....)))))))))))))).))..))))..)))))...))).............((((.....(((((....(..(((.((..(((((((((((.((..............)))))))))))))....)).)))..)....)))))))))....))).....))))))).....))))))).))))...))............. (-193.34) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUGUUAAUUUUUCCUUUCUUCUUAAACUGCUGGGAAUAAGCUAUCUAGGUAAAGCAAGAUUUCUACUCAAACUCCUCUUUCCCAAUAAACACAGAUGGAUAGUGUAGUAAUUCAUUGAGAUCGGUAUCCAAUGGUAAAUCAUAAAUUGCKAAAUAGUUUUCAGGAUAUAGAGCAACUCUUUCCAGCACACUGGGGUCAUAGCUGUUUCCUGAUCUAGGUGACCAUCCUAGCACAGGGGGAUCCUCUAGAGUCGACCUGCAGGCAUGCAAGCUCUGCAUUAAUGAAUCGGCCAACGCGCGGGGAGAGGCGGUUUGCGUAUUGGGCGCUCUUCCGCUUCCUCGCUCACUGACUCGCUGCGCUCGGUCGUUCGGCUGCGGCGAGCGGUAUCAGCUCACUCAAAGGCGGUAAUACGGUUAUCCACAGAAUCAGGGGAUAACGCAGGAAAGAACAUUCGGGCAGCGUUGGGUCCUGGCCCACGGGUGCGCAUGAUCGUGCUCCUGUCGUUGAGGACCCNNNNNUUAAUUAAUUAANNNNNUCAUGACAUAACCUAUAAAAUAGGCGUAUCACGAGCCCUUUCGUCUCGCGCGUUUCGGUGAUGACGGUGAAACUUCUGACACAUGCAGCUCCCGGAGACGGUCACAGCUUGUCUGUAAGCGGAUGCCG ........(((((((.............((((((((....(.(((((((.....((((......((((((...((((((..............))).)))...)).))))..((((((.(((....)))))))))...........))))..........((((((...(.(((......((((((....)))))).....))).).)))))).))))))).)..))))))))..(.(..(((((((.((.((....(((((((......)).))))).........(.(((.....(((((((..(((((...((((.....))))....))))))))))))..((((.((((((((((.(((....))))).))))))))))))...............))).)......((....))...)).)))))))))..).).)))))))...((((((.(((...(((((((.(.(.(((((.((((......)))).))))).).).))))))).......................((((......(((((...)))))....(((.(((.....(((((((((.((...)))))).))))).....))).)))..))))..)))))))))..((((....(((((....)))))....)))) (-207.34) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1223 330 281 217 168 smit:SMT_1982|5end_conserved_hypothetical_protein_1913036..1913266_POSITIVE 1223 330 281 205 156 smit:SMT_1691|5end_putative_deoxyribonuclease_1674978..1675208_POSITIVE 1223 330 281 230 181 smit:SMT_1156|5end_K07485_transposase_1099938..1100168_POSITIVE 1203 370 321 208 159 smit:SMT_325|5end_K02029_polar_amino_acid_transport_system_permease_protein_263023..263253_NEGATIVE 1182 340 291 200 151 smit:SMT_1972|5end_uncharacterized_conserved_phage_related_protein_1903662..1903892_POSITIVE 1174 380 331 185 136 smit:SMT_1986|5end_mercuric_reductase_1914843..1915073_POSITIVE 1022 440 391 192 143 smit:SMT_959|5end_1-acyl-sn-glycerol-3-phosphate_acyltransferase_905283..905513_POSITIVE 917 420 375 229 184 smit:SMT_1914|5end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1859486..1859716_POSITIVE 775 380 341 230 192 smit:SMT_2045|5end_K+_transport_system,_NAD-binding_component_1956139..1956369_POSITIVE 654 451 420 231 200 smit:SMT_2005|5end_pullulanase,_type_I_1926289..1926519_NEGATIVE 439 100 56 134 83 smit:SMT_2052|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1961591..1961821_NEGATIVE 432 463 436 231 204 smit:SMT_712|5end_alcohol_dehydrogenase,_zinc-containing_620381..620611_NEGATIVE 407 490 441 219 166 smit:SMT_389|5end_PTS_system,_galactitol-specific_IIA_component,_putative_321779..322009_POSITIVE 405 210 162 138 81 smit:SMT_2162|5end_hypothetical_protein_2036931..2037161_POSITIVE 405 486 441 194 146 smit:SMT_543|5end_topoisomerase_IV_subunit_B_458372..458602_POSITIVE 397 486 465 178 157 smit:SMT_1671|5end_hypothetical_protein_1659757..1659987_NEGATIVE 396 408 361 137 76 smit:SMT_941|5end_hypothetical_protein_893558..893788_NEGATIVE 395 281 260 231 210 smit:SMT_2009|5end_GtrA_family_protein_1928973..1929203_POSITIVE 389 369 322 208 160 smit:SMT_2002|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1924405..1924635_NEGATIVE 389 369 322 208 160 smit:SMT_1942|5end_zinc_metalloprotease_ZmpB,_truncated_1877882..1878112_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1112 450 401 89 40 smit:SMT_2024|3end_hypothetical_protein_1940690..1940840_POSITIVE 1112 450 401 78 29 smit:SMT_2002|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1924145..1924295_NEGATIVE 1112 450 401 89 40 smit:SMT_1981|3end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1912868..1913018_POSITIVE 1112 450 401 94 45 smit:SMT_1971|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1903526..1903676_POSITIVE 1112 450 401 78 29 smit:SMT_1966|3end_DNA_polymerase_III_alpha_subunit_1898328..1898478_POSITIVE 1112 450 401 89 40 smit:SMT_1942|3end_zinc_metalloprotease_ZmpB,_truncated_1878384..1878534_POSITIVE 1112 450 401 78 29 smit:SMT_1927|3end_cell_division_protein_FtsH_1868408..1868558_NEGATIVE 1112 450 401 89 40 smit:SMT_1926|3end_mevalonate_kinase_1868062..1868212_POSITIVE 1112 450 401 89 40 smit:SMT_1887|3end_LicC_protein_1841740..1841890_POSITIVE 1112 450 401 78 29 smit:SMT_1885|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1839830..1839980_NEGATIVE 1112 450 401 78 29 smit:SMT_1873|3end_K02006_cobalt_transport_system_ATP-binding_protein_1832351..1832501_POSITIVE 1112 450 401 94 45 smit:SMT_1852|3end_1,4-alpha-glucan_branching_enzyme_1816467..1816617_NEGATIVE 1112 450 401 94 45 smit:SMT_326|3end_K02029_polar_amino_acid_transport_system_permease_protein_263217..263367_NEGATIVE 1111 50 1 81 32 smit:SMT_1946|3end_sucrose-6-phosphate_hydrolase_1881205..1881355_POSITIVE 1023 480 431 51 2 smit:SMT_2030|3end_chromosomal_replication_initiator_protein_DnaA_1944790..1944940_NEGATIVE 1023 480 431 55 6 smit:SMT_479|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_415604..415754_NEGATIVE 1022 440 391 68 19 smit:SMT_958|3end_hypothetical_protein_predicted_by_Glimmer/Critica_905239..905389_POSITIVE 946 480 434 47 1 smit:SMT_1895|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1847402..1847552_POSITIVE 843 486 441 130 85 smit:SMT_713|3end_heat_shock_protein_HtpX_620505..620655_NEGATIVE 730 486 449 38 1 smit:SMT_1857|3end_hypothetical_protein_1821145..1821295_POSITIVE