Origin IGS: agccactcccatagtcgagagtgcaaaacgtttaatagannnnnttaattaattaannnnnttacgccagaattcgagctcggtacccctgagtctaggtcggactagaccttgtaaaacgacggccagtccagtgtgctggaaagcaagctcgtcaacaagcagaagctgacgatgttgagatccgtctccacagtattatctacaaggttatcgaagag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctcttcgataaccttgtagataatactgtggagacggatctcaacatcgtcagcttctgcttgttgacgagcttgctttccagcacactggactggccgtcgttttacaaggtctagtccgacctagactcaggggtaccgagctcgaattctggcgtaannnnnttaattaattaannnnntctattaaacgttttgcactctcgactatgggagtggct Mask Tandem Repeat Region ================================================ agccactcccatagtcgagagtgcaaaacgtttaatagannnnnttaattaattaannnnnttacgccagaattcgagctcggtacccctgagtctaggtcggactagaccttgtaaaacgacggccagtccagtgtgctggaaagcaagctcgtcaacaagcagaagctgacgatgttgagatccgtctccacagtattatctacaaggttatcgaagag Find is-nt database================================================ Query_seq: SMT_1872:SMT_1873|SMT_1872:SMT_1873:K03217 preprotein translocase YidC subunit:K02006 cobalt transport system ATP-binding protein:<-->:1831855..1832075 221 agccactcccatagtcgagagtgcaaaacgtttaatagannnnnttaattaattaannnnnttacgccagaattcgagctcggtacccctgagtctaggtcggactagaccttgtaaaacgacggccagtccagtgtgctggaaagcaagctcgtcaacaagcagaagctgacgatgttgagatccgtctccacagtattatctacaaggttatcgaagag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1872:SMT_1873|SMT_1872:SMT_1873:K03217 preprotein translocase YidC subunit:K02006 cobalt transport system ATP-binding protein:<-->:1831855..1832075 221 agccactcccatagtcgagagtgcaaaacgtttaatagannnnnttaattaattaannnnnttacgccagaattcgagctcggtacccctgagtctaggtcggactagaccttgtaaaacgacggccagtccagtgtgctggaaagcaagctcgtcaacaagcagaagctgacgatgttgagatccgtctccacagtattatctacaaggttatcgaagag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1872:SMT_1873|SMT_1872:SMT_1873:K03217 preprotein translocase YidC subunit:K02006 cobalt transport system ATP-binding protein:<-->:1831855..1832075 221 agccactcccatagtcgagagtgcaaaacgtttaatagannnnnttaattaattaannnnnttacgccagaattcgagctcggtacccctgagtctaggtcggactagaccttgtaaaacgacggccagtccagtgtgctggaaagcaagctcgtcaacaagcagaagctgacgatgttgagatccgtctccacagtattatctacaaggttatcgaagag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agccactcccatagtcgagagtgcaaaacgtttaatagannnnnttaattaattaannnnnttacgccagaattcgagctcggtacccctgagtctaggtcggactagaccttgtaaaacgacggccagtccagtgtgctggaaagcaagctcgtcaacaagcagaagctgacgatgttgagatccgtctccacagtattatctacaaggttatcgaagag Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: - Start: 52 End: 165 ................................................... N X X X R W F E L E T G R L R P R V L G Q L V V A L G T H Q F A L E D V L M ........................................................ agccactcccatagtcgagagtgcaaaacgtttaatagannnnnttaattaattaannnnnttacgccagaattcgagctcggtacccctgagtctaggtcggactagaccttgtaaaacgacggccagtccagtgtgctggaaagcaagctcgtcaacaagcagaagctgacgatgttgagatccgtctccacagtattatctacaaggttatcgaagag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agccactcccatagtcgagagtgcaaaacgtttaatagannnnnttaattaattaannnnnttacgccagaattcgagctcggtacccctgagtctaggtcggactagaccttgtaaaacgacggccagtccagtgtgctggaaagcaagctcgtcaacaagcagaagctgacgatgttgagatccgtctccacagtattatctacaaggttatcgaagag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1872:SMT_1873|SMT_1872:SMT_1873:K03217 preprotein translocase YidC subunit:K02006 cobalt transport system ATP-binding protein:<-->:1831855..1832075 221 agccactcccatagtcgagagtgcaaaacgtttaatagannnnnttaattaattaannnnnttacgccagaattcgagctcggtacccctgagtctaggtcggactagaccttgtaaaacgacggccagtccagtgtgctggaaagcaagctcgtcaacaagcagaagctgacgatgttgagatccgtctccacagtattatctacaaggttatcgaagag Intra-Species Hit: Count: 1 Min: 1 Max: 221 Len: 221 Subject: smit_SMT_1872_SMT_1873|K03217 preprotein translocase YidC subunit:K02006 cobalt transport system ATP-binding protein|NEGATIVE:POSITIVE|[1831855,1832075]|221 HSP 1 e-value: 1.0E-104 bit: 379.0 Len: 221 Query Start:1 Query End:221 Subject Strand: POSITIVE Subject Start: 1 Subject End: 221 agccactcccatagtcgagagtgcaaaacgtttaatagannnnnttaattaattaannnnnttacgccagaattcgagctcggtacccctgagtctaggtcggactagaccttgtaaaacgacggccagtccagtgtgctggaaagcaagctcgtcaacaagcagaagctgacgatgttgagatccgtctccacagtattatctacaaggttatcgaagag agccactcccatagtcgagagtgcaaaacgtttaatagannnnnttaattaattaannnnnttacgccagaattcgagctcggtacccctgagtctaggtcggactagaccttgtaaaacgacggccagtccagtgtgctggaaagcaagctcgtcaacaagcagaagctgacgatgttgagatccgtctccacagtattatctacaaggttatcgaagag Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGCCACUCCCAUAGUCGAGAGUGCAAAACGUUUAAUAGANNNNNUUAAUUAAUUAANNNNNUUACGCCAGAAUUCGAGCUCGGUACCCCUGAGUCUAGGUCGGACUAGACCUUGUAAAACGACGGCCAGUCCAGUGUGCUGGAAAGCAAGCUCGUCAACAAGCAGAAGCUGACGAUGUUGAGAUCCGUCUCCACAGUAUUAUCUACAAGGUUAUCGAAGAG .((.((((.(......).))))))................................................(((((....(((.((((((....))))..))))).(((((((((....((((((...(((((....)))))......)).))))....(((....))).....(((.((((....)))).)))........))))))))).)))))... (-49.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUCUUCGAUAACCUUGUAGAUAAUACUGUGGAGACGGAUCUCAACAUCGUCAGCUUCUGCUUGUUGACGAGCUUGCUUUCCAGCACACUGGACUGGCCGUCGUUUUACAAGGUCUAGUCCGACCUAGACUCAGGGGUACCGAGCUCGAAUUCUGGCGUAANNNNNUUAAUUAAUUAANNNNNUCUAUUAAACGUUUUGCACUCUCGACUAUGGGAGUGGCU ....(((.((.(((((..........(((((((((((.((.(((..((((((((........))))))))..)))...(((((....)))))..)))))))..)))))).(((((((......)))))))))))).)).)))(((.(....).)))..........................................(((((((......)))))))... (-56.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1054 130 81 221 172 smit:SMT_1872|5end_K03217_preprotein_translocase_YidC_subunit_1831764..1831994_NEGATIVE 1054 130 81 198 149 smit:SMT_389|5end_PTS_system,_galactitol-specific_IIA_component,_putative_321779..322009_POSITIVE 1033 140 91 188 139 smit:SMT_543|5end_topoisomerase_IV_subunit_B_458372..458602_POSITIVE 957 107 62 231 186 smit:SMT_1465|5end_hypothetical_protein_1445409..1445639_POSITIVE 927 105 62 231 188 smit:SMT_2025|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1941765..1941995_NEGATIVE 743 158 113 51 1 smit:SMT_956|5end_hypothetical_protein_predicted_by_Glimmer/Critica_904789..905019_NEGATIVE 641 152 115 152 118 smit:SMT_1983|5end_glycerophosphoryl_diester_phosphodiesterase_1913691..1913921_NEGATIVE 620 153 119 32 1 smit:SMT_1957|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1890645..1890875_POSITIVE 453 130 86 198 151 smit:SMT_1116|5end_K02244_competence_protein_ComGB_1053841..1054071_NEGATIVE 372 157 111 178 122 smit:SMT_1887|5end_LicC_protein_1841445..1841675_POSITIVE 344 170 126 153 100 smit:SMT_1694|5end_GTPase_1676674..1676904_POSITIVE 339 147 101 81 28 smit:SMT_1942|5end_zinc_metalloprotease_ZmpB,_truncated_1877882..1878112_POSITIVE 338 108 62 155 113 smit:SMT_181|5end_50S_ribosomal_protein_L24_120125..120355_POSITIVE 337 164 123 204 172 smit:SMT_1129|5end_hypothetical_protein_1063926..1064156_NEGATIVE 335 179 131 204 163 smit:SMT_1671|5end_hypothetical_protein_1659757..1659987_NEGATIVE 331 173 134 224 190 smit:SMT_2052|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1961591..1961821_NEGATIVE 328 102 66 231 195 smit:SMT_1249|5end_hypothetical_protein_1210795..1211025_NEGATIVE 328 109 67 49 3 smit:SMT_687|5end_carbamoyl-phosphate_synthase,_large_subunit_599264..599494_NEGATIVE 322 147 101 231 181 smit:SMT_360|5end_K07742_hypothetical_protein_295628..295858_POSITIVE 316 165 126 42 1 smit:SMT_841|5end_dihydrodipicolinate_reductase_788025..788255_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1054 130 81 116 67 smit:SMT_2044|3end_transcriptional_activator_TipA,_putative_1955274..1955424_NEGATIVE 1054 130 81 116 67 smit:SMT_2026|3end_K03466_DNA_segregation_ATPase_FtsK/SpoIIIE,_S-DNA-T_family_1941905..1942055_NEGATIVE 1054 130 81 116 67 smit:SMT_2016|3end_tyrosine_recombinase_1933021..1933171_NEGATIVE 1054 130 81 116 67 smit:SMT_2006|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1926803..1926953_POSITIVE 1054 130 81 126 77 smit:SMT_2001|3end_membrane_protease_protein_family_1923889..1924039_POSITIVE 1054 130 81 126 77 smit:SMT_1985|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1914407..1914557_NEGATIVE 1054 130 81 116 67 smit:SMT_1957|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1890829..1890979_POSITIVE 1054 130 81 74 25 smit:SMT_1955|3end_carbamoyl-phosphate_synthase_small_chain_1888992..1889142_NEGATIVE 1054 130 81 116 67 smit:SMT_1886|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1841198..1841348_POSITIVE 1054 130 81 116 67 smit:SMT_1658|3end_hypothetical_protein_1648937..1649087_POSITIVE 1054 130 81 72 23 smit:SMT_1631|3end_phosphopantothenoylcysteine_synthase/decarboxylase_1618204..1618354_NEGATIVE 1054 130 81 116 67 smit:SMT_1558|3end_hypothetical_protein_1543855..1544005_NEGATIVE 1054 130 81 126 77 smit:SMT_1464|3end_K02030_polar_amino_acid_transport_system_substrate-binding_protein_1445361..1445511_POSITIVE 1054 130 81 116 67 smit:SMT_1155|3end_ABC-type_oligopeptide_transport_system,_periplasmic_component_1099183..1099333_NEGATIVE 1033 140 91 58 9 smit:SMT_2045|3end_K+_transport_system,_NAD-binding_component_1956758..1956908_POSITIVE 1033 140 91 136 87 smit:SMT_542|3end_hypothetical_protein_458400..458550_POSITIVE 935 146 101 47 2 smit:SMT_957|3end_UDP-glucose_4-epimerase_904776..904926_NEGATIVE 935 146 101 132 87 smit:SMT_956|3end_hypothetical_protein_predicted_by_Glimmer/Critica_904691..904841_NEGATIVE 693 152 112 145 108 smit:SMT_1954|3end_6-phospho-beta-glucosidase_1888831..1888981_POSITIVE 641 152 115 138 104 smit:SMT_1984|3end_phosphinothricin_N-acetyltransferase_1913705..1913855_NEGATIVE