Origin IGS: cattttggagagattaaaaagttttttgaaccttacagcacaaaacgaggttctcacttgctttttaaatcatctccaattttttatcttacaatcctatcaaacacggtttcataggtaactaaggaaacaaac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gtttgtttccttagttacctatgaaaccgtgtttgataggattgtaagataaaaaattggagatgatttaaaaagcaagtgagaacctcgttttgtgctgtaaggttcaaaaaactttttaatctctccaaaatg Mask Tandem Repeat Region ================================================ cattttggagagattaaaaagttttttgaaccttacagcacaaaacgaggttctcacttgctttttaaatcatctccaattttttatcttacaatcctatcaaacacggtttcataggtaactaaggaaacaaac Find is-nt database================================================ Query_seq: SMT_1810:SMT_1811|SMT_1810:SMT_1811:hypothetical protein:hypothetical protein:->->:1785659..1785793 135 cattttggagagattaaaaagttttttgaaccttacagcacaaaacgaggttctcacttgctttttaaatcatctccaattttttatcttacaatcctatcaaacacggtttcataggtaactaaggaaacaaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1810:SMT_1811|SMT_1810:SMT_1811:hypothetical protein:hypothetical protein:->->:1785659..1785793 135 cattttggagagattaaaaagttttttgaaccttacagcacaaaacgaggttctcacttgctttttaaatcatctccaattttttatcttacaatcctatcaaacacggtttcataggtaactaaggaaacaaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1810:SMT_1811|SMT_1810:SMT_1811:hypothetical protein:hypothetical protein:->->:1785659..1785793 135 cattttggagagattaaaaagttttttgaaccttacagcacaaaacgaggttctcacttgctttttaaatcatctccaattttttatcttacaatcctatcaaacacggtttcataggtaactaaggaaacaaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cattttggagagattaaaaagttttttgaaccttacagcacaaaacgaggttctcacttgctttttaaatcatctccaattttttatcttacaatcctatcaaacacggtttcataggtaactaaggaaacaaac Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 13 End: 114 ............ M K K F F E P Y S T K R G S H L L F K S S P I F Y L T I L S N T V S ..................... Protein_Len: 39 Strand: + Start: 2 End: 118 . M L E R L K S F L N L T A Q N E V L T C F L N H L Q F F I L Q S Y Q T R F H R ................. cattttggagagattaaaaagttttttgaaccttacagcacaaaacgaggttctcacttgctttttaaatcatctccaattttttatcttacaatcctatcaaacacggtttcataggtaactaaggaaacaaac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cattttggagagattaaaaagttttttgaaccttacagcacaaaacgaggttctcacttgctttttaaatcatctccaattttttatcttacaatcctatcaaacacggtttcataggtaactaaggaaacaaac Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1810:SMT_1811|SMT_1810:SMT_1811:hypothetical protein:hypothetical protein:->->:1785659..1785793 135 cattttggagagattaaaaagttttttgaaccttacagcacaaaacgaggttctcacttgctttttaaatcatctccaattttttatcttacaatcctatcaaacacggtttcataggtaactaaggaaacaaac Intra-Species Hit: Count: 1 Min: 1 Max: 135 Len: 135 Subject: smit_SMT_1810_SMT_1811|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1785659,1785793]|135 HSP 1 e-value: 4.0E-71 bit: 268.0 Len: 135 Query Start:1 Query End:135 Subject Strand: POSITIVE Subject Start: 1 Subject End: 135 cattttggagagattaaaaagttttttgaaccttacagcacaaaacgaggttctcacttgctttttaaatcatctccaattttttatcttacaatcctatcaaacacggtttcataggtaactaaggaaacaaac cattttggagagattaaaaagttttttgaaccttacagcacaaaacgaggttctcacttgctttttaaatcatctccaattttttatcttacaatcctatcaaacacggtttcataggtaactaaggaaacaaac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAUUUUGGAGAGAUUAAAAAGUUUUUUGAACCUUACAGCACAAAACGAGGUUCUCACUUGCUUUUUAAAUCAUCUCCAAUUUUUUAUCUUACAAUCCUAUCAAACACGGUUUCAUAGGUAACUAAGGAAACAAAC ....((((((((((((((((((.....(((((((............)))))))......))))))).)))).))))))).......(((((....(((((.((((...)))).)))))....)))))........ (-33.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUUGUUUCCUUAGUUACCUAUGAAACCGUGUUUGAUAGGAUUGUAAGAUAAAAAAUUGGAGAUGAUUUAAAAAGCAAGUGAGAACCUCGUUUUGUGCUGUAAGGUUCAAAAAACUUUUUAAUCUCUCCAAAAUG .(((((((...((((..(((((.((((...)))).)))))))))..)))))))...(((((((.((((.((((((.......((((((..(........)..))))))......))))))))))))))))).... (-32.42) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 283 118 72 139 97 smit:SMT_711|5end_choline_kinase_620170..620400_NEGATIVE 283 118 72 139 97 smit:SMT_681|5end_choline_kinase_590821..591051_NEGATIVE 256 119 73 140 98 smit:SMT_1374|5end_uridylate_kinase_1353020..1353250_NEGATIVE 255 65 39 177 147 smit:SMT_1365|5end_translation_initiation_factor_IF-3_1344485..1344715_NEGATIVE 237 107 61 215 156 smit:SMT_1442|5end_hypothetical_protein_1426328..1426558_NEGATIVE 230 85 41 69 16 smit:SMT_1987|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1916325..1916555_NEGATIVE 223 87 51 125 95 smit:SMT_1898|5end_aminotransferase_(class_V),_putative_1849929..1850159_NEGATIVE 222 68 30 196 156 smit:SMT_1734|5end_serine/threonine_protein_phosphatase_1718558..1718788_POSITIVE 218 65 21 175 124 smit:SMT_680|5end_choline_transport_protein_589962..590192_NEGATIVE 214 110 65 146 91 smit:SMT_380|5end_hypothetical_protein_316376..316606_POSITIVE 214 110 65 146 91 smit:SMT_371|5end_hypothetical_protein_305248..305478_POSITIVE 210 76 31 139 88 smit:SMT_1797|5end_threonine_dehydratase_1772684..1772914_NEGATIVE 210 75 36 71 31 smit:SMT_332|5end_phosphoglycerate_kinase_271425..271655_POSITIVE 210 91 57 116 70 smit:SMT_210|5end_acetyltransferase,_GNAT_family_143380..143610_POSITIVE 208 86 45 86 39 smit:SMT_609|5end_laminin-binding_surface_protein_521286..521516_POSITIVE 206 98 54 124 72 smit:SMT_908|5end_hypothetical_protein_857230..857460_NEGATIVE 205 79 31 221 177 smit:SMT_1645|5end_hypothetical_protein_1631738..1631968_POSITIVE 205 43 3 227 181 smit:SMT_1093|5end_K03073_preprotein_translocase_SecE_subunit_1033926..1034156_NEGATIVE 204 114 71 131 82 smit:SMT_301|5end_hypothetical_protein_244086..244316_POSITIVE 204 86 49 68 28 smit:SMT_113|5end_hypothetical_protein_61425..61655_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 283 118 72 122 80 smit:SMT_712|3end_alcohol_dehydrogenase,_zinc-containing_620187..620337_NEGATIVE 283 118 72 122 80 smit:SMT_682|3end_hypothetical_protein_590838..590988_NEGATIVE 256 119 73 56 14 smit:SMT_1375|3end_putative_glucose-inhibited_division_protein_1353104..1353254_NEGATIVE 242 46 2 66 31 smit:SMT_631|3end_glycerate_kinase_537307..537457_POSITIVE 237 128 108 137 118 smit:SMT_774|3end_hypothetical_protein_684479..684629_NEGATIVE 235 129 86 33 1 smit:SMT_140|3end_NUDIX_family_hydrolase_91498..91648_POSITIVE 225 66 31 149 114 smit:SMT_2027|3end_competence-specific_global_transcription_modulator_1943025..1943175_NEGATIVE 225 66 31 149 114 smit:SMT_1091|3end_competence-specific_global_transcription_modulator_1032561..1032711_NEGATIVE 225 66 31 149 114 smit:SMT_102|3end_competence-specific_global_transcription_modulator_34667..34817_POSITIVE 221 77 36 133 92 smit:SMT_544|3end_aminoglycoside_6-adenylyltransferase_461190..461340_POSITIVE 214 110 65 146 91 smit:SMT_379|3end_acetyltransferase,_GNAT_family_316456..316606_POSITIVE 214 110 65 146 91 smit:SMT_370|3end_acetyltransferase,_GNAT_family_305328..305478_POSITIVE 210 91 57 104 58 smit:SMT_209|3end_acetyltransferase,_GNAT_family_143448..143598_POSITIVE 208 79 31 70 11 smit:SMT_337|3end_hypothetical_protein_276197..276347_POSITIVE 206 98 54 131 79 smit:SMT_909|3end_type_I_restriction_enzyme_M_protein_857223..857373_NEGATIVE 204 114 71 78 29 smit:SMT_300|3end_hypothetical_protein_244113..244263_POSITIVE 204 65 21 83 37 smit:SMT_101|3end_cell_division_protein_34067..34217_POSITIVE 200 66 31 149 113 smit:SMT_2048|3end_competence-specific_global_transcription_modulator_1957961..1958111_NEGATIVE 199 77 46 141 102 smit:SMT_1935|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1873930..1874080_POSITIVE 199 77 46 141 102 smit:SMT_1314|3end_dTDP-4-dehydrorhamnose_3,5-epimerase_related_enzyme_1280192..1280342_POSITIVE