Origin IGS: gattctgatcctccagattccttttattgctaatattataccacattttcagattttctctttaagaaaggcatttataagaaaaacgttcgttttttatctatttccaaaaagcctcaaaactaaatgtcaaaactaaattgatagaatttttagaaaatttctggttttttctttacagaaaaaaaaattctgctataatgactcacataattaaattaggatttaagggagacgatatgacatctgctaaagatatatccaaagcgtgttttgaacnnnnnttaattaattaannnnntgtccttctcctattttcttatttacgatatttaattgtatacgcttttaattgtatacgtttttatgctttttgtcaataaaaaaacatatattcaataaaataaacgcccttatttttacttgctatcttattaaagtttataatatataggtttttcattgccgaaaacaatttttatagttctttaaagcaactgtattattaaatcttattcatgtaataatattcatatttgtcagatgctgattctcgataatctagacatattctagtcttatttcatattatttattccactgcaaaacaaaaaaatttgacaaaaaaatcttctgctctaaaccatttctaacgagtatttttctacttttagacttttcctgtctacaccagcattcaatcatttggtcaacttgtactatttatttactttatcaatattcctttttatttcttcaaacctataaaaagatgagtagttaggaaacaatagtatcctatcttccaacattttccactgtttttatcttcttagtatcttatcactcttctcagttaactcagtataaggcttctcattagtattgattattttttctaattcctattatatttagccaaatttcagattagctatatataaaatagtagatttcaaaaaactccttacatataacaagtaattttactcccttactataaactgaatcaaaaaacataggtactataatgagttatgatagaaatagcgtaaccgatttcctaagtccataggaatygccccctttcttcatcttcattatagcacctatacatcaattgtgcaaataatatgatatttttttattagtcc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ggactaataaaaaaatatcatattatttgcacaattgatgtataggtgctataatgaagatgaagaaagggggcrattcctatggacttaggaaatcggttacgctatttctatcataactcattatagtacctatgttttttgattcagtttatagtaagggagtaaaattacttgttatatgtaaggagttttttgaaatctactattttatatatagctaatctgaaatttggctaaatataataggaattagaaaaaataatcaatactaatgagaagccttatactgagttaactgagaagagtgataagatactaagaagataaaaacagtggaaaatgttggaagataggatactattgtttcctaactactcatctttttataggtttgaagaaataaaaaggaatattgataaagtaaataaatagtacaagttgaccaaatgattgaatgctggtgtagacaggaaaagtctaaaagtagaaaaatactcgttagaaatggtttagagcagaagatttttttgtcaaatttttttgttttgcagtggaataaataatatgaaataagactagaatatgtctagattatcgagaatcagcatctgacaaatatgaatattattacatgaataagatttaataatacagttgctttaaagaactataaaaattgttttcggcaatgaaaaacctatatattataaactttaataagatagcaagtaaaaataagggcgtttattttattgaatatatgtttttttattgacaaaaagcataaaaacgtatacaattaaaagcgtatacaattaaatatcgtaaataagaaaataggagaaggacannnnnttaattaattaannnnngttcaaaacacgctttggatatatctttagcagatgtcatatcgtctcccttaaatcctaatttaattatgtgagtcattatagcagaatttttttttctgtaaagaaaaaaccagaaattttctaaaaattctatcaatttagttttgacatttagttttgaggctttttggaaatagataaaaaacgaacgtttttcttataaatgcctttcttaaagagaaaatctgaaaatgtggtataatattagcaataaaaggaatctggaggatcagaatc Mask Tandem Repeat Region ================================================ gattctgatcctccagattccttttattgctaatattataccacattttcagattttctctttaagaaaggcatttataagaaaaacgttcgttttttatctatttccaaaaagccnnnnnnnnnnnnnnnnnnnnnnnnntgatagaatttttagaaaatttctggttttttctttacagaaaaaaaaattctgctataatgactcacataattaaattaggatttaagggagacgatatgacatctgctaaagatatatccaaagcgtgttttgaacnnnnnttaattaattaannnnntgtccttctcctattttcttatttacgatannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgctttttgtcaataaaaaaacatatattcaataaaataaacgcccttatttttacttgctatcttattaaagtttataatatataggtttttcattgccgaaaacaatttttatagttctttaaagcaactgtattattaaatcttattcatgtaataatattcatatttgtcagatgctgattctcgataatctagacatattctagtcttatttcatattatttattccactgcaaaacaaaaaaatttgacaaaaaaatcttctgctctaaaccatttctaacgagtatttttctacttttagacttttcctgtctacaccagcattcaatcatttggtcaacttgtactatttatttactttatcaatattcctttttatttcttcaaacctataaaaagatgagtagttaggaaacaatagtatcctatcttccaacattttccactgtttttatcttcttagtatcttatcactcttctcagttaactcagtataaggcttctcattagtattgattattttttctaattcctattatatttagccaaatttcagattagctatatataaaatagtagatttcaaaaaactccttacatataacaagtaattttactcccttactataaactgaatcaaaaaacataggtactataatgagttatgatagaaatagcgtaaccgatttcctaagtccataggaatygccccctttcttcatcttcattatagcacctatacatcaattgtgcaaataatatgatatttttttattagtcc Find is-nt database================================================ Query_seq: SMT_1796:SMT_1797|SMT_1796:SMT_1797:dihydroorotate dehydrogenase A:threonine dehydratase:<-<-:1770907..1772060 1154 gattctgatcctccagattccttttattgctaatattataccacattttcagattttctctttaagaaaggcatttataagaaaaacgttcgttttttatctatttccaaaaagcctcaaaactaaatgtcaaaactaaattgatagaatttttagaaaatttctggttttttctttacagaaaaaaaaattctgctataatgactcacataattaaattaggatttaagggagacgatatgacatctgctaaagatatatccaaagcgtgttttgaacnnnnnttaattaattaannnnntgtccttctcctattttcttatttacgatatttaattgtatacgcttttaattgtatacgtttttatgctttttgtcaataaaaaaacatatattcaataaaataaacgcccttatttttacttgctatcttattaaagtttataatatataggtttttcattgccgaaaacaatttttatagttctttaaagcaactgtattattaaatcttattcatgtaataatattcatatttgtcagatgctgattctcgataatctagacatattctagtcttatttcatattatttattccactgcaaaacaaaaaaatttgacaaaaaaatcttctgctctaaaccatttctaacgagtatttttctacttttagacttttcctgtctacaccagcattcaatcatttggtcaacttgtactatttatttactttatcaatattcctttttatttcttcaaacctataaaaagatgagtagttaggaaacaatagtatcctatcttccaacattttccactgtttttatcttcttagtatcttatcactcttctcagttaactcagtataaggcttctcattagtattgattattttttctaattcctattatatttagccaaatttcagattagctatatataaaatagtagatttcaaaaaactccttacatataacaagtaattttactcccttactataaactgaatcaaaaaacataggtactataatgagttatgatagaaatagcgtaaccgatttcctaagtccataggaatygccccctttcttcatcttcattatagcacctatacatcaattgtgcaaataatatgatatttttttattagtcc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1796:SMT_1797|SMT_1796:SMT_1797:dihydroorotate dehydrogenase A:threonine dehydratase:<-<-:1770907..1772060 1154 gattctgatcctccagattccttttattgctaatattataccacattttcagattttctctttaagaaaggcatttataagaaaaacgttcgttttttatctatttccaaaaagcctcaaaactaaatgtcaaaactaaattgatagaatttttagaaaatttctggttttttctttacagaaaaaaaaattctgctataatgactcacataattaaattaggatttaagggagacgatatgacatctgctaaagatatatccaaagcgtgttttgaacnnnnnttaattaattaannnnntgtccttctcctattttcttatttacgatatttaattgtatacgcttttaattgtatacgtttttatgctttttgtcaataaaaaaacatatattcaataaaataaacgcccttatttttacttgctatcttattaaagtttataatatataggtttttcattgccgaaaacaatttttatagttctttaaagcaactgtattattaaatcttattcatgtaataatattcatatttgtcagatgctgattctcgataatctagacatattctagtcttatttcatattatttattccactgcaaaacaaaaaaatttgacaaaaaaatcttctgctctaaaccatttctaacgagtatttttctacttttagacttttcctgtctacaccagcattcaatcatttggtcaacttgtactatttatttactttatcaatattcctttttatttcttcaaacctataaaaagatgagtagttaggaaacaatagtatcctatcttccaacattttccactgtttttatcttcttagtatcttatcactcttctcagttaactcagtataaggcttctcattagtattgattattttttctaattcctattatatttagccaaatttcagattagctatatataaaatagtagatttcaaaaaactccttacatataacaagtaattttactcccttactataaactgaatcaaaaaacataggtactataatgagttatgatagaaatagcgtaaccgatttcctaagtccataggaatygccccctttcttcatcttcattatagcacctatacatcaattgtgcaaataatatgatatttttttattagtcc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1796:SMT_1797|SMT_1796:SMT_1797:dihydroorotate dehydrogenase A:threonine dehydratase:<-<-:1770907..1772060 1154 gattctgatcctccagattccttttattgctaatattataccacattttcagattttctctttaagaaaggcatttataagaaaaacgttcgttttttatctatttccaaaaagcctcaaaactaaatgtcaaaactaaattgatagaatttttagaaaatttctggttttttctttacagaaaaaaaaattctgctataatgactcacataattaaattaggatttaagggagacgatatgacatctgctaaagatatatccaaagcgtgttttgaacnnnnnttaattaattaannnnntgtccttctcctattttcttatttacgatatttaattgtatacgcttttaattgtatacgtttttatgctttttgtcaataaaaaaacatatattcaataaaataaacgcccttatttttacttgctatcttattaaagtttataatatataggtttttcattgccgaaaacaatttttatagttctttaaagcaactgtattattaaatcttattcatgtaataatattcatatttgtcagatgctgattctcgataatctagacatattctagtcttatttcatattatttattccactgcaaaacaaaaaaatttgacaaaaaaatcttctgctctaaaccatttctaacgagtatttttctacttttagacttttcctgtctacaccagcattcaatcatttggtcaacttgtactatttatttactttatcaatattcctttttatttcttcaaacctataaaaagatgagtagttaggaaacaatagtatcctatcttccaacattttccactgtttttatcttcttagtatcttatcactcttctcagttaactcagtataaggcttctcattagtattgattattttttctaattcctattatatttagccaaatttcagattagctatatataaaatagtagatttcaaaaaactccttacatataacaagtaattttactcccttactataaactgaatcaaaaaacataggtactataatgagttatgatagaaatagcgtaaccgatttcctaagtccataggaatygccccctttcttcatcttcattatagcacctatacatcaattgtgcaaataatatgatatttttttattagtcc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gattctgatcctccagattccttttattgctaatattataccacattttcagattttctctttaagaaaggcatttataagaaaaacgttcgttttttatctatttccaaaaagcctcaaaactaaatgtcaaaactaaattgatagaatttttagaaaatttctggttttttctttacagaaaaaaaaattctgctataatgactcacataattaaattaggatttaagggagacgatatgacatctgctaaagatatatccaaagcgtgttttgaacnnnnnttaattaattaannnnntgtccttctcctattttcttatttacgatatttaattgtatacgcttttaattgtatacgtttttatgctttttgtcaataaaaaaacatatattcaataaaataaacgcccttatttttacttgctatcttattaaagtttataatatataggtttttcattgccgaaaacaatttttatagttctttaaagcaactgtattattaaatcttattcatgtaataatattcatatttgtcagatgctgattctcgataatctagacatattctagtcttatttcatattatttattccactgcaaaacaaaaaaatttgacaaaaaaatcttctgctctaaaccatttctaacgagtatttttctacttttagacttttcctgtctacaccagcattcaatcatttggtcaacttgtactatttatttactttatcaatattcctttttatttcttcaaacctataaaaagatgagtagttaggaaacaatagtatcctatcttccaacattttccactgtttttatcttcttagtatcttatcactcttctcagttaactcagtataaggcttctcattagtattgattattttttctaattcctattatatttagccaaatttcagattagctatatataaaatagtagatttcaaaaaactccttacatataacaagtaattttactcccttactataaactgaatcaaaaaacataggtactataatgagttatgatagaaatagcgtaaccgatttcctaagtccataggaatygccccctttcttcatcttcattatagcacctatacatcaattgtgcaaataatatgatatttttttattagtcc Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 2 End: 136 . M L I L Q I P F I A N I I P H F Q I F S L R K A F I R K T F V F Y L F P K S L K T K C Q N .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 99 End: 293 .................................................................................................. M Y F Q K A S K L N V K T K L I E F L E N F W F F L Y R K K N S A I M T H I I K L G F K G D D M T S A K D I S K A C F E ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 33 Strand: + Start: 353 End: 451 ................................................................................................................................................................................................................................................................................................................................................................ M Y T F L C F L S I K K H I F N K I N A L I F T C Y L I K V Y N I ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 40 Strand: + Start: 646 End: 765 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M S N E Y F S T F R L F L S T P A F N H L V N L Y Y L F T L S I F L F I S S N L ..................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 581 End: 781 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M S Y Y L F H C K T K K F D K K I F C S K P F L T S I F L L L D F S C L H Q H S I I W S T C T I Y L L Y Q Y S F L F L Q ..................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 30 Strand: + Start: 791 End: 880 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M V S Y L P T F S T V F I F L V S Y H S S Q L T Q Y K A S H .................................................................................................................................................................................................................................................................................. Protein_Len: 33 Strand: - Start: 934 End: 1032 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... S Y I F Y Y I E F F E K C I V L L K V G K S Y V S D F F M P V I M .......................................................................................................................... Protein_Len: 30 Strand: - Start: 723 End: 812 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. K N V K D I N R K I E E F R Y F S S Y N P F L L I R D E L M ...................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 44 Strand: - Start: 592 End: 723 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... K N W Q L V F F N S L F I K Q E L G N R V L I K R S K S K E Q R C W C E I M Q D V Q V M ............................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 32 Strand: - Start: 380 End: 475 ........................................................................................................................................................................................................................................................................................................................................................................................... Y F F M Y E I F Y V G K N K S A I K N F N I I Y L N K M A S F M ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... gattctgatcctccagattccttttattgctaatattataccacattttcagattttctctttaagaaaggcatttataagaaaaacgttcgttttttatctatttccaaaaagcctcaaaactaaatgtcaaaactaaattgatagaatttttagaaaatttctggttttttctttacagaaaaaaaaattctgctataatgactcacataattaaattaggatttaagggagacgatatgacatctgctaaagatatatccaaagcgtgttttgaacnnnnnttaattaattaannnnntgtccttctcctattttcttatttacgatatttaattgtatacgcttttaattgtatacgtttttatgctttttgtcaataaaaaaacatatattcaataaaataaacgcccttatttttacttgctatcttattaaagtttataatatataggtttttcattgccgaaaacaatttttatagttctttaaagcaactgtattattaaatcttattcatgtaataatattcatatttgtcagatgctgattctcgataatctagacatattctagtcttatttcatattatttattccactgcaaaacaaaaaaatttgacaaaaaaatcttctgctctaaaccatttctaacgagtatttttctacttttagacttttcctgtctacaccagcattcaatcatttggtcaacttgtactatttatttactttatcaatattcctttttatttcttcaaacctataaaaagatgagtagttaggaaacaatagtatcctatcttccaacattttccactgtttttatcttcttagtatcttatcactcttctcagttaactcagtataaggcttctcattagtattgattattttttctaattcctattatatttagccaaatttcagattagctatatataaaatagtagatttcaaaaaactccttacatataacaagtaattttactcccttactataaactgaatcaaaaaacataggtactataatgagttatgatagaaatagcgtaaccgatttcctaagtccataggaatygccccctttcttcatcttcattatagcacctatacatcaattgtgcaaataatatgatatttttttattagtcc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 88 Start: 352 End: 381 ...............................................................................................................................................................................................................................................................................................................................................................ATTGACAAAAAGCATAAAAACGTATACAAT..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... PromScan Matrix: RpoD-17 Strand: - Score: 86 Start: 1099 End: 1128 ..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTGCACAATTGATGTATAGGTGCTATAAT.......................... PromScan Matrix: RpoD-17 Strand: + Score: 82 Start: 477 End: 506 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTTATAGTTCTTTAAAGCAACTGTATTAT........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 419 End: 448 ..................................................................................................................................................................................................................................................................................................................................................................................................................................TTTACTTGCTATCTTATTAAAGTTTATAAT.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 456 End: 485 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTTTCATTGCCGAAAACAATTTTTATAGT............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gattctgatcctccagattccttttattgctaatattataccacattttcagattttctctttaagaaaggcatttataagaaaaacgttcgttttttatctatttccaaaaagcctcaaaactaaatgtcaaaactaaattgatagaatttttagaaaatttctggttttttctttacagaaaaaaaaattctgctataatgactcacataattaaattaggatttaagggagacgatatgacatctgctaaagatatatccaaagcgtgttttgaacnnnnnttaattaattaannnnntgtccttctcctattttcttatttacgatatttaattgtatacgcttttaattgtatacgtttttatgctttttgtcaataaaaaaacatatattcaataaaataaacgcccttatttttacttgctatcttattaaagtttataatatataggtttttcattgccgaaaacaatttttatagttctttaaagcaactgtattattaaatcttattcatgtaataatattcatatttgtcagatgctgattctcgataatctagacatattctagtcttatttcatattatttattccactgcaaaacaaaaaaatttgacaaaaaaatcttctgctctaaaccatttctaacgagtatttttctacttttagacttttcctgtctacaccagcattcaatcatttggtcaacttgtactatttatttactttatcaatattcctttttatttcttcaaacctataaaaagatgagtagttaggaaacaatagtatcctatcttccaacattttccactgtttttatcttcttagtatcttatcactcttctcagttaactcagtataaggcttctcattagtattgattattttttctaattcctattatatttagccaaatttcagattagctatatataaaatagtagatttcaaaaaactccttacatataacaagtaattttactcccttactataaactgaatcaaaaaacataggtactataatgagttatgatagaaatagcgtaaccgatttcctaagtccataggaatygccccctttcttcatcttcattatagcacctatacatcaattgtgcaaataatatgatatttttttattagtcc Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 55 HP_score: -4.3 Tail_Score: -3.63045 Start: 562 End: 576 Full_Region: CTGATTCTCGATAAT CTAGA CATAT TCTAG TCTTATTTCATATTA .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CTAGACATATTCTAG.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 48 HP_score: -4.3 Tail_Score: -2.57931 Start: 562 End: 576 Full_Region: taatatgaaataaga ctaga atatg tctag attatcgagaatcag .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ctagaatatgtctag.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Find igs database================================================ Query_seq: SMT_1796:SMT_1797|SMT_1796:SMT_1797:dihydroorotate dehydrogenase A:threonine dehydratase:<-<-:1770907..1772060 1154 gattctgatcctccagattccttttattgctaatattataccacattttcagattttctctttaagaaaggcatttataagaaaaacgttcgttttttatctatttccaaaaagcctcaaaactaaatgtcaaaactaaattgatagaatttttagaaaatttctggttttttctttacagaaaaaaaaattctgctataatgactcacataattaaattaggatttaagggagacgatatgacatctgctaaagatatatccaaagcgtgttttgaacnnnnnttaattaattaannnnntgtccttctcctattttcttatttacgatatttaattgtatacgcttttaattgtatacgtttttatgctttttgtcaataaaaaaacatatattcaataaaataaacgcccttatttttacttgctatcttattaaagtttataatatataggtttttcattgccgaaaacaatttttatagttctttaaagcaactgtattattaaatcttattcatgtaataatattcatatttgtcagatgctgattctcgataatctagacatattctagtcttatttcatattatttattccactgcaaaacaaaaaaatttgacaaaaaaatcttctgctctaaaccatttctaacgagtatttttctacttttagacttttcctgtctacaccagcattcaatcatttggtcaacttgtactatttatttactttatcaatattcctttttatttcttcaaacctataaaaagatgagtagttaggaaacaatagtatcctatcttccaacattttccactgtttttatcttcttagtatcttatcactcttctcagttaactcagtataaggcttctcattagtattgattattttttctaattcctattatatttagccaaatttcagattagctatatataaaatagtagatttcaaaaaactccttacatataacaagtaattttactcccttactataaactgaatcaaaaaacataggtactataatgagttatgatagaaatagcgtaaccgatttcctaagtccataggaatygccccctttcttcatcttcattatagcacctatacatcaattgtgcaaataatatgatatttttttattagtcc Intra-Species Hit: Count: 1 Min: 1 Max: 1139 Len: 1139 Subject: smit_SMT_1796_SMT_1797|dihydroorotate dehydrogenase A:threonine dehydratase|NEGATIVE:NEGATIVE|[1770907,1772060]|1154 HSP 1 e-value: 0.0 bit: 1043.0 Len: 604 Query Start:1 Query End:604 Subject Strand: POSITIVE Subject Start: 1 Subject End: 604 gattctgatcctccagattccttttattgctaatattataccacattttcagattttctctttaagaaaggcatttataagaaaaacgttcgttttttatctatttccaaaaagcctcaaaactaaatgtcaaaactaaattgatagaatttttagaaaatttctggttttttctttacagnnnnnnnnnttctgctataatgactcacataattaaattaggatttaagggagacgatatgacatctgctaaagatatatccaaagcgtgttttgaacnnnnnttaattaattaannnnntgtccttctcctattttcttatttacgatatttaattgtatacgcttttaattgtatacgtttttatgctttttgtcaatnnnnnnncatatattcaataaaataaacgcccttatttttacttgctatcttattaaagtttataatatataggtttttcattgccgaaaacaatttttatagttctttaaagcaactgtattattaaatcttattcatgtaataatattcatatttgtcagatgctgattctcgataatctagacatattctagtcttatttcatattatttattccactgc...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... gattctgatcctccagattccttttattgctaatattataccacattttcagattttctctttaagaaaggcatttataagaaaaacgttcgttttttatctatttccaaaaagcctcaaaactaaatgtcaaaactaaattgatagaatttttagaaaatttctggttttttctttacagaaaaaaaaattctgctataatgactcacataattaaattaggatttaagggagacgatatgacatctgctaaagatatatccaaagcgtgttttgaacnnnnnttaattaattaannnnntgtccttctcctattttcttatttacgatatttaattgtatacgcttttaattgtatacgtttttatgctttttgtcaataaaaaaacatatattcaataaaataaacgcccttatttttacttgctatcttattaaagtttataatatataggtttttcattgccgaaaacaatttttatagttctttaaagcaactgtattattaaatcttattcatgtaataatattcatatttgtcagatgctgattctcgataatctagacatattctagtcttatttcatattatttattccactgc...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 0.0 bit: 1008.0 Len: 510 Query Start:630 Query End:1139 Subject Strand: POSITIVE Subject Start: 630 Subject End: 1139 .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tcttctgctctaaaccatttctaacgagtatttttctacttttagacttttcctgtctacaccagcattcaatcatttggtcaacttgtactatttatttactttatcaatattcctttttatttcttcaaacctataaaaagatgagtagttaggaaacaatagtatcctatcttccaacattttccactgtttttatcttcttagtatcttatcactcttctcagttaactcagtataaggcttctcattagtattgattattttttctaattcctattatatttagccaaatttcagattagctatatataaaatagtagatttcaaaaaactccttacatataacaagtaattttactcccttactataaactgaatcaaaaaacataggtactataatgagttatgatagaaatagcgtaaccgatttcctaagtccataggaatygccccctttcttcatcttcattatagcacctatacatcaattgtgcaaataatatgata............... .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tcttctgctctaaaccatttctaacgagtatttttctacttttagacttttcctgtctacaccagcattcaatcatttggtcaacttgtactatttatttactttatcaatattcctttttatttcttcaaacctataaaaagatgagtagttaggaaacaatagtatcctatcttccaacattttccactgtttttatcttcttagtatcttatcactcttctcagttaactcagtataaggcttctcattagtattgattattttttctaattcctattatatttagccaaatttcagattagctatatataaaatagtagatttcaaaaaactccttacatataacaagtaattttactcccttactataaactgaatcaaaaaacataggtactataatgagttatgatagaaatagcgtaaccgatttcctaagtccataggaatygccccctttcttcatcttcattatagcacctatacatcaattgtgcaaataatatgata............... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUUCUGAUCCUCCAGAUUCCUUUUAUUGCUAAUAUUAUACCACAUUUUCAGAUUUUCUCUUUAAGAAAGGCAUUUAUAAGAAAAACGUUCGUUUUUUAUCUAUUUCCAAAAAGCCUCAAAACUAAAUGUCAAAACUAAAUUGAUAGAAUUUUUAGAAAAUUUCUGGUUUUUUCUUUACAGAAAAAAAAAUUCUGCUAUAAUGACUCACAUAAUUAAAUUAGGAUUUAAGGGAGACGAUAUGACAUCUGCUAAAGAUAUAUCCAAAGCGUGUUUUGAACNNNNNUUAAUUAAUUAANNNNNUGUCCUUCUCCUAUUUUCUUAUUUACGAUAUUUAAUUGUAUACGCUUUUAAUUGUAUACGUUUUUAUGCUUUUUGUCAAUAAAAAAACAUAUAUUCAAUAAAAUAAACGCCCUUAUUUUUACUUGCUAUCUUAUUAAAGUUUAUAAUAUAUAGGUUUUUCAUUGCCGAAAACAAUUUUUAUAGUUCUUUAAAGCAACUGUAUUAUUAAAUCUUAUUCAUGUAAUAAUAUUCAUAUUUGUCAGAUGCUGAUUCUCGAUAAUCUAGACAUAUUCUAGUCUUAUUUCAUAUUAUUUAUUCCACUGCAAAACAAAAAAAUUUGACAAAAAAAUCUUCUGCUCUAAACCAUUUCUAACGAGUAUUUUUCUACUUUUAGACUUUUCCUGUCUACACCAGCAUUCAAUCAUUUGGUCAACUUGUACUAUUUAUUUACUUUAUCAAUAUUCCUUUUUAUUUCUUCAAACCUAUAAAAAGAUGAGUAGUUAGGAAACAAUAGUAUCCUAUCUUCCAACAUUUUCCACUGUUUUUAUCUUCUUAGUAUCUUAUCACUCUUCUCAGUUAACUCAGUAUAAGGCUUCUCAUUAGUAUUGAUUAUUUUUUCUAAUUCCUAUUAUAUUUAGCCAAAUUUCAGAUUAGCUAUAUAUAAAAUAGUAGAUUUCAAAAAACUCCUUACAUAUAACAAGUAAUUUUACUCCCUUACUAUAAACUGAAUCAAAAAACAUAGGUACUAUAAUGAGUUAUGAUAGAAAUAGCGUAACCGAUUUCCUAAGUCCAUAGGAAUYGCCCCCUUUCUUCAUCUUCAUUAUAGCACCUAUACAUCAAUUGUGCAAAUAAUAUGAUA ((.((((......)))).))............(((((((..((((..(((((..........((((...(((.(((((..(((((((....)))))))...((((..((((((((........((((((((.............((((((((((......((((((............)))))).))))))))))...................(((((.((((.....((((((.((((....((((.....)))).....(((((....))))).........................))))..))))))...)))).))))).))))))))...(((((((........)))))))........))))))))..)))).....................))))).)))))))...(((((((.(((.....((((((..((((......(((((......((.(((.........(((((((((....)).))))))).................................((((((((((..((((....(((((.(((((.....)))))..)))))....))))..........((.....)).....))))))))))......))).))...))))).......((((((.............(((((.......))))).(((((...........)))))..)))))).....))))..)))))).........((((((((............))))))))..((((...(((((.((....)).))))).............((.((((((((........((((((((..........((.((....)).)).....((((......(((((..(((((.......)))))..))))).....)))).......))))..)))).....)))))))).)).........)))).......))).)))))))....................))))).........((((((.((((((((((..((((.(((((..(.((.......((((((......))))))..)).)..))))))))).)))))))))).))))))........))))...))))))).... (-183.52) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUCAUAUUAUUUGCACAAUUGAUGUAUAGGUGCUAUAAUGAAGAUGAAGAAAGGGGGCRAUUCCUAUGGACUUAGGAAAUCGGUUACGCUAUUUCUAUCAUAACUCAUUAUAGUACCUAUGUUUUUUGAUUCAGUUUAUAGUAAGGGAGUAAAAUUACUUGUUAUAUGUAAGGAGUUUUUUGAAAUCUACUAUUUUAUAUAUAGCUAAUCUGAAAUUUGGCUAAAUAUAAUAGGAAUUAGAAAAAAUAAUCAAUACUAAUGAGAAGCCUUAUACUGAGUUAACUGAGAAGAGUGAUAAGAUACUAAGAAGAUAAAAACAGUGGAAAAUGUUGGAAGAUAGGAUACUAUUGUUUCCUAACUACUCAUCUUUUUAUAGGUUUGAAGAAAUAAAAAGGAAUAUUGAUAAAGUAAAUAAAUAGUACAAGUUGACCAAAUGAUUGAAUGCUGGUGUAGACAGGAAAAGUCUAAAAGUAGAAAAAUACUCGUUAGAAAUGGUUUAGAGCAGAAGAUUUUUUUGUCAAAUUUUUUUGUUUUGCAGUGGAAUAAAUAAUAUGAAAUAAGACUAGAAUAUGUCUAGAUUAUCGAGAAUCAGCAUCUGACAAAUAUGAAUAUUAUUACAUGAAUAAGAUUUAAUAAUACAGUUGCUUUAAAGAACUAUAAAAAUUGUUUUCGGCAAUGAAAAACCUAUAUAUUAUAAACUUUAAUAAGAUAGCAAGUAAAAAUAAGGGCGUUUAUUUUAUUGAAUAUAUGUUUUUUUAUUGACAAAAAGCAUAAAAACGUAUACAAUUAAAAGCGUAUACAAUUAAAUAUCGUAAAUAAGAAAAUAGGAGAAGGACANNNNNUUAAUUAAUUAANNNNNGUUCAAAACACGCUUUGGAUAUAUCUUUAGCAGAUGUCAUAUCGUCUCCCUUAAAUCCUAAUUUAAUUAUGUGAGUCAUUAUAGCAGAAUUUUUUUUUCUGUAAAGAAAAAACCAGAAAUUUUCUAAAAAUUCUAUCAAUUUAGUUUUGACAUUUAGUUUUGAGGCUUUUUGGAAAUAGAUAAAAAACGAACGUUUUUCUUAUAAAUGCCUUUCUUAAAGAGAAAAUCUGAAAAUGUGGUAUAAUAUUAGCAAUAAAAGGAAUCUGGAGGAUCAGAAUC ..(((((((((((((((.((((((.(((((((((((((((((..(((((((((...(((..((((((......)))))).........))).))))).))))...)))))))))))))))))(((((((((((.....((((((.((...(((((..((((.(((....))).)))).)))))...)))))))).((((((.(((((((........))))))).))))))...)))))))))))....)))))).........((((((....((.((....)).))................(((((((((...((((.(....).))))...((.(((((.((....)).))))).))....))))))))).))))))...((((((((((((..((((.....))))...............(((((.(((.((((.....(((.(.(((((........(((((.((((......))))..)))))....))))).).)))..)))).))).))))).))))))))))))...)))...))))))))))))....((((........)))).((((.((.(((.((......((.(.(((((......((((((((.....((((((.....(((.((((((....(((((..........))))).)))))).......((((.(((((.......)))))..))))...))).....((((((((((((......(((..(.((((((((((((..((((((((...(((((.((((.(((((((.((((((........))).)))...........(((((.((((....................................(((..(((....)))..))).((((......))))..))))...))))).))))))).)))).((((...(((.(((((((((((((((....)))))))....)))))))))))......(((......)))...)))).....)))))...))))))))..))))))...)))))).)..))).....))))))))))))..........))))))....))))))))..))))).))).......)).))).)).))))...... (-239.02) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 782 50 1 141 92 smit:SMT_1796|5end_dihydroorotate_dehydrogenase_A_1770816..1771046_NEGATIVE 507 278 241 178 141 smit:SMT_717|5end_Mn2+_and_Fe2+_transporter_of_the_NRAMP_family_623147..623377_NEGATIVE 302 1127 1081 90 45 smit:SMT_521|5end_K02005_HlyD_family_secretion_protein_445566..445796_POSITIVE 290 887 841 136 88 smit:SMT_408|5end_hypothetical_protein_353087..353317_POSITIVE 284 666 633 222 183 smit:SMT_1840|5end_6-phosphogluconate_dehydrogenase,_decarboxylating_1806285..1806515_NEGATIVE 283 52 12 140 104 smit:SMT_953|5end_hypothetical_protein_901576..901806_NEGATIVE 277 1101 1068 122 86 smit:SMT_1377|5end_hypothetical_protein_1355873..1356103_NEGATIVE 275 57 11 144 98 smit:SMT_1355|5end_conserved_hypothetical_protein_TIGR00043_1332479..1332709_NEGATIVE 273 876 833 40 1 smit:SMT_1739|5end_hypothetical_protein_1721263..1721493_POSITIVE 269 901 861 112 66 smit:SMT_1292|5end_K02050_sulfonate/nitrate/taurine_transport_system_permease_protein_1254179..1254409_POSITIVE 264 833 793 180 137 smit:SMT_999|5end_hypothetical_protein_predicted_by_Glimmer/Critica_941642..941872_NEGATIVE 258 1089 1050 113 61 smit:SMT_1691|5end_putative_deoxyribonuclease_1674978..1675208_POSITIVE 256 789 742 221 177 smit:SMT_1734|5end_serine/threonine_protein_phosphatase_1718558..1718788_POSITIVE 255 1108 1066 128 85 smit:SMT_1075|5end_hypothetical_protein_1013473..1013703_POSITIVE 255 710 661 210 156 smit:SMT_794|5end_hypothetical_protein_703487..703717_NEGATIVE 250 580 535 201 153 smit:SMT_1332|5end_hypothetical_protein_1307576..1307806_NEGATIVE 250 857 813 155 97 smit:SMT_905|5end_hypothetical_protein_predicted_by_Glimmer/Critica_853467..853697_NEGATIVE 249 1110 1061 171 122 smit:SMT_2088|5end_glucose-1-phosphate_thymidylyltransferase_1984624..1984854_POSITIVE 249 1094 1057 109 73 smit:SMT_1651|5end_chromosome_segregation_SMC_protein_1636998..1637228_POSITIVE 248 1124 1081 194 150 smit:SMT_639|5end_6-phospho-beta-galactosidase_544885..545115_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 293 698 656 142 93 smit:SMT_1061|3end_hypothetical_protein_999915..1000065_POSITIVE 290 887 841 135 87 smit:SMT_463|3end_para-aminobenzoate_synthetase_component_I_/_4-amino-4-deoxychorismate_lyase_402839..402989_POSITIVE 290 887 841 135 87 smit:SMT_407|3end_para-aminobenzoate_synthetase_component_I_/_4-amino-4-deoxychorismate_lyase_353166..353316_POSITIVE 284 666 633 146 107 smit:SMT_1841|3end_hypothetical_protein_1806361..1806511_NEGATIVE 284 666 633 146 107 smit:SMT_303|3end_hypothetical_protein_245758..245908_POSITIVE 283 52 12 39 3 smit:SMT_954|3end_cation-transporting_ATPase,_E1-E2_family_901677..901827_NEGATIVE 278 1107 1061 104 51 smit:SMT_766|3end_hypothetical_protein_673385..673535_NEGATIVE 277 1101 1068 82 46 smit:SMT_1378|3end_DNA_polymerase_III,_delta'_subunit_1355913..1356063_NEGATIVE 271 1110 1061 150 101 smit:SMT_278|3end_tyrosine-protein_kinase_221048..221198_POSITIVE 270 876 842 38 8 smit:SMT_1738|3end_peptidyl-prolyl_cis-trans_isomerase_1721341..1721491_POSITIVE 269 901 861 149 103 smit:SMT_1291|3end_hypothetical_protein_1254296..1254446_POSITIVE 261 718 673 54 15 smit:SMT_1071|3end_hypothetical_protein_1010386..1010536_POSITIVE 252 856 811 80 37 smit:SMT_1069|3end_acetyl_xylan_esterase,_putative_1009595..1009745_POSITIVE 249 1094 1057 118 82 smit:SMT_1650|3end_ribonuclease_III_1637087..1637237_POSITIVE 249 576 543 141 107 smit:SMT_410|3end_glucokinase_355477..355627_POSITIVE 245 837 795 60 14 smit:SMT_2033|3end_hydrolase,_haloacid_dehalogenase-like_family_1946739..1946889_POSITIVE 245 1064 1025 86 51 smit:SMT_1906|3end_peptidyl-tRNA_hydrolase_1853723..1853873_NEGATIVE 243 717 680 142 94 smit:SMT_1540|3end_hypothetical_protein_1524307..1524457_NEGATIVE 243 708 662 140 94 smit:SMT_1203|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1164221..1164371_NEGATIVE 241 719 675 137 90 smit:SMT_109|3end_beta-galactosidase_57523..57673_POSITIVE