Origin IGS: cctagaagttcctatctaatagctctctgtatatgtgagctactttttccagagaatagcgagaggccaaagctagatttttttcatttaatagataaaaamcaggctgttccatcttttctatcttttgaaatcactctgatttggtttaaacmagttgatggcattggactggnnnnnttaattaattaannnnntggtcattctgcargtgtcttgcgtaaattgctgagattcgtgctcacttcccagatcgtact .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agtacgatctgggaagtgagcacgaatctcagcaatttacgcaagacacytgcagaatgaccannnnnttaattaattaannnnnccagtccaatgccatcaactkgtttaaaccaaatcagagtgatttcaaaagatagaaaagatggaacagcctgktttttatctattaaatgaaaaaaatctagctttggcctctcgctattctctggaaaaagtagctcacatatacagagagctattagataggaacttctagg Mask Tandem Repeat Region ================================================ cctagaagttcctatctaatagctctctgtatatgtgagctactttttccagagaatagcgagaggccaaagctagatttttttcatttaatagataaaaamcaggctgttccatcttttctatcttttgaaatcactctgatttggtttaaacmagttgatggcattggactggnnnnnttaattaattaannnnntggtcattctgcargtgtcttgcgtaaattgctgagattcgtgctcacttcccagatcgtact Find is-nt database================================================ Query_seq: SMT_1792:SMT_1793|SMT_1792:SMT_1793:K03307 solute:Na+ symporter, SSS family:inosine-5'-monophosphate dehydrogenase:<-->:1767865..1768124 260 cctagaagttcctatctaatagctctctgtatatgtgagctactttttccagagaatagcgagaggccaaagctagatttttttcatttaatagataaaaamcaggctgttccatcttttctatcttttgaaatcactctgatttggtttaaacmagttgatggcattggactggnnnnnttaattaattaannnnntggtcattctgcargtgtcttgcgtaaattgctgagattcgtgctcacttcccagatcgtact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1792:SMT_1793|SMT_1792:SMT_1793:K03307 solute:Na+ symporter, SSS family:inosine-5'-monophosphate dehydrogenase:<-->:1767865..1768124 260 cctagaagttcctatctaatagctctctgtatatgtgagctactttttccagagaatagcgagaggccaaagctagatttttttcatttaatagataaaaamcaggctgttccatcttttctatcttttgaaatcactctgatttggtttaaacmagttgatggcattggactggnnnnnttaattaattaannnnntggtcattctgcargtgtcttgcgtaaattgctgagattcgtgctcacttcccagatcgtact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1792:SMT_1793|SMT_1792:SMT_1793:K03307 solute:Na+ symporter, SSS family:inosine-5'-monophosphate dehydrogenase:<-->:1767865..1768124 260 cctagaagttcctatctaatagctctctgtatatgtgagctactttttccagagaatagcgagaggccaaagctagatttttttcatttaatagataaaaamcaggctgttccatcttttctatcttttgaaatcactctgatttggtttaaacmagttgatggcattggactggnnnnnttaattaattaannnnntggtcattctgcargtgtcttgcgtaaattgctgagattcgtgctcacttcccagatcgtact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cctagaagttcctatctaatagctctctgtatatgtgagctactttttccagagaatagcgagaggccaaagctagatttttttcatttaatagataaaaamcaggctgttccatcttttctatcttttgaaatcactctgatttggtttaaacmagttgatggcattggactggnnnnnttaattaattaannnnntggtcattctgcargtgtcttgcgtaaattgctgagattcgtgctcacttcccagatcgtact Predict ORF larger than 30AA ================================================ Protein_Len: 57 Strand: + Start: 19 End: 189 .................. M A L C I C E L L F P E N S E R P K L D F F H L I D K X Q A V P S F L S F E I T L I W F K X V D G I G L X X L I N ....................................................................... Protein_Len: 33 Strand: - Start: 87 End: 185 ...................................................................................... K I S L F X A T G D K R D K S I V R I Q N L X T S P M P S X X X M ........................................................................... Protein_Len: 41 Strand: - Start: 5 End: 124 .... * F N R D L L E R Y I H A V K E L S Y R S A L A L N K E N L L Y F X P Q E M K E M ........................................................................................................................................ cctagaagttcctatctaatagctctctgtatatgtgagctactttttccagagaatagcgagaggccaaagctagatttttttcatttaatagataaaaamcaggctgttccatcttttctatcttttgaaatcactctgatttggtttaaacmagttgatggcattggactggnnnnnttaattaattaannnnntggtcattctgcargtgtcttgcgtaaattgctgagattcgtgctcacttcccagatcgtact Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cctagaagttcctatctaatagctctctgtatatgtgagctactttttccagagaatagcgagaggccaaagctagatttttttcatttaatagataaaaamcaggctgttccatcttttctatcttttgaaatcactctgatttggtttaaacmagttgatggcattggactggnnnnnttaattaattaannnnntggtcattctgcargtgtcttgcgtaaattgctgagattcgtgctcacttcccagatcgtact Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 46 HP_score: -3.4 Tail_Score: -3.06133 Start: 20 End: 42 Full_Region: attctctggaaaaag tagctcac atataca gagagcta ttagataggaacttc ...................tagctcacatatacagagagcta.......................................................................................................................................................................................................................... Find igs database================================================ Query_seq: SMT_1792:SMT_1793|SMT_1792:SMT_1793:K03307 solute:Na+ symporter, SSS family:inosine-5'-monophosphate dehydrogenase:<-->:1767865..1768124 260 cctagaagttcctatctaatagctctctgtatatgtgagctactttttccagagaatagcgagaggccaaagctagatttttttcatttaatagataaaaamcaggctgttccatcttttctatcttttgaaatcactctgatttggtttaaacmagttgatggcattggactggnnnnnttaattaattaannnnntggtcattctgcargtgtcttgcgtaaattgctgagattcgtgctcacttcccagatcgtact Intra-Species Hit: Count: 1 Min: 1 Max: 260 Len: 260 Subject: smit_SMT_1792_SMT_1793|K03307 solute:Na+ symporter, SSS family:inosine-5'-monophosphate dehydrogenase|NEGATIVE:POSITIVE|[1767865,1768124]|260 HSP 1 e-value: 1.0E-109 bit: 394.0 Len: 260 Query Start:1 Query End:260 Subject Strand: POSITIVE Subject Start: 1 Subject End: 260 cctagaagttcctatctaatagctctctgtatatgtgagctactttttccagagaatagcgagaggccaaagctagannnnnnncatttaatagataaaaamcaggctgttccatcttttctatcttttgaaatcactctgatttggtttaaacmagttgatggcattggactnnnnnnnttaattaattaannnnntggtcattctgcargtgtcttgcgtaaattgctgagattcgtgctcacttcccagatcgtact cctagaagttcctatctaatagctctctgtatatgtgagctactttttccagagaatagcgagaggccaaagctagatttttttcatttaatagataaaaamcaggctgttccatcttttctatcttttgaaatcactctgatttggtttaaacmagttgatggcattggactggnnnnnttaattaattaannnnntggtcattctgcargtgtcttgcgtaaattgctgagattcgtgctcacttcccagatcgtact Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CCUAGAAGUUCCUAUCUAAUAGCUCUCUGUAUAUGUGAGCUACUUUUUCCAGAGAAUAGCGAGAGGCCAAAGCUAGAUUUUUUUCAUUUAAUAGAUAAAAAMCAGGCUGUUCCAUCUUUUCUAUCUUUUGAAAUCACUCUGAUUUGGUUUAAACMAGUUGAUGGCAUUGGACUGGNNNNNUUAAUUAAUUAANNNNNUGGUCAUUCUGCARGUGUCUUGCGUAAAUUGCUGAGAUUCGUGCUCACUUCCCAGAUCGUACU ..((((........))))...((..((((.....((((((.((....(((((......((((((.((..((((((((((.......(((((.(((((((((...((.....))...)))).))))).)))))........)))))))))).............(((...(((((......((((....)))).....)))))....)))..)).))))))........))).))...))))))))....))))..))... (-49.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUACGAUCUGGGAAGUGAGCACGAAUCUCAGCAAUUUACGCAAGACACYUGCAGAAUGACCANNNNNUUAAUUAAUUAANNNNNCCAGUCCAAUGCCAUCAACUKGUUUAAACCAAAUCAGAGUGAUUUCAAAAGAUAGAAAAGAUGGAACAGCCUGKUUUUUAUCUAUUAAAUGAAAAAAAUCUAGCUUUGGCCUCUCGCUAUUCUCUGGAAAAAGUAGCUCACAUAUACAGAGAGCUAUUAGAUAGGAACUUCUAGG .....((.((((....((((.......))))(((.....(....).....)))................................)))))).....(((((..((.((((.....)))).)).....((((........)))).))))).....((((.((((((((((..............(((((...((((.....))))....)))))....(((((((.(.......).)))))))))))))))))....)))) (-43.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 943 70 21 161 112 smit:SMT_1792|5end_K03307_solute:Na+_symporter,_SSS_family_1767774..1768004_NEGATIVE 943 70 21 161 112 smit:SMT_1175|5end_K03307_solute:Na+_symporter,_SSS_family_1127660..1127890_NEGATIVE 315 52 11 51 8 smit:SMT_356|5end_hypothetical_protein_292394..292624_POSITIVE 292 69 27 172 130 smit:SMT_835|5end_polypeptide_deformylase_777549..777779_NEGATIVE 289 250 207 159 114 smit:SMT_923|5end_methionyl-tRNA_formyltransferase_877494..877724_NEGATIVE 289 249 205 153 110 smit:SMT_921|5end_putative_PP2C_protein_phosphatase_875238..875468_NEGATIVE 283 243 201 202 151 smit:SMT_722|5end_Tn5252,_Orf_9_protein_629106..629336_NEGATIVE 278 256 215 132 103 smit:SMT_1541|5end_isopentenyl-diphosphate_delta-isomerase_1526093..1526323_NEGATIVE 273 67 21 207 146 smit:SMT_1692|5end_hypothetical_protein_1675169..1675399_POSITIVE 271 75 39 166 124 smit:SMT_1078|5end_folylpolyglutamate_synthase_1016713..1016943_NEGATIVE 270 90 43 90 49 smit:SMT_359|5end_transcription_termination-antitermination_factor_294470..294700_POSITIVE 267 73 38 123 87 smit:SMT_2083|5end_K06147_ATP-binding_cassette,_subfamily_B,_bacterial_1982411..1982641_POSITIVE 264 69 21 203 160 smit:SMT_1331|5end_hypothetical_protein_1306236..1306466_NEGATIVE 262 69 21 146 105 smit:SMT_2038|5end_zinc_metalloprotease_ZmpB,_truncated_1948303..1948533_POSITIVE 262 92 51 160 127 smit:SMT_91|5end_hypothetical_protein_22578..22808_POSITIVE 261 141 105 83 47 smit:SMT_1870|5end_K07566_putative_translation_factor_1829819..1830049_NEGATIVE 258 85 44 145 98 smit:SMT_1993|5end_hypothetical_protein_1919800..1920030_NEGATIVE 256 69 21 221 176 smit:SMT_1756|5end_hypothetical_protein_1734170..1734400_NEGATIVE 256 77 39 179 142 smit:SMT_755|5end_shikimate_5-dehydrogenase_659917..660147_NEGATIVE 256 68 22 189 142 smit:SMT_749|5end_shikimate_kinase_653971..654201_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 868 60 11 149 100 smit:SMT_1176|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1127662..1127812_NEGATIVE 292 69 27 57 15 smit:SMT_836|3end_cell_surface_protein_precursor_777664..777814_NEGATIVE 289 250 207 146 101 smit:SMT_924|3end_primosomal_protein_N'_877507..877657_NEGATIVE 289 249 205 138 95 smit:SMT_922|3end_RNA-binding_protein_875253..875403_NEGATIVE 282 68 28 118 78 smit:SMT_1303|3end_tRNA-dihydrouridine_synthase_1264676..1264826_NEGATIVE 278 256 215 148 119 smit:SMT_1542|3end_phosphomevalonate_kinase_1526077..1526227_NEGATIVE 274 66 21 108 60 smit:SMT_1775|3end_K07478_putative_ATPase_1751315..1751465_NEGATIVE 270 90 43 46 5 smit:SMT_358|3end_hypothetical_protein_294506..294656_POSITIVE 262 92 51 95 62 smit:SMT_90|3end_DNA_polymerase_III_beta_subunit_22593..22743_POSITIVE 261 141 105 99 63 smit:SMT_1871|3end_modification_methylase,_HemK_family_1829803..1829953_NEGATIVE 261 129 103 35 8 smit:SMT_987|3end_N-acetylmannosamine_kinase_/_transcriptional_regulator_931655..931805_NEGATIVE 253 63 21 56 10 smit:SMT_1082|3end_Cof_family_protein_1020877..1021027_POSITIVE 252 175 133 121 82 smit:SMT_2014|3end_tryptophan_synthase_beta_chain_1932727..1932877_POSITIVE 252 80 49 148 117 smit:SMT_833|3end_hypothetical_protein_predicted_by_Glimmer/Critica_775183..775333_NEGATIVE 249 69 23 54 2 smit:SMT_1951|3end_ABC_transporter,_ATP-binding_protein_1885141..1885291_NEGATIVE 248 246 203 92 50 smit:SMT_2123|3end_hypothetical_protein_2008541..2008691_POSITIVE 248 247 201 55 10 smit:SMT_1938|3end_zinc_metalloprotease_ZmpB,_putative_1876230..1876380_POSITIVE 248 246 203 62 20 smit:SMT_752|3end_prephenate_dehydrogenase_655688..655838_NEGATIVE 247 68 22 148 97 smit:SMT_773|3end_UDP-N-acetylenolpyruvoylglucosamine_reductase_683460..683610_NEGATIVE 247 79 49 38 7 smit:SMT_198|3end_50S_ribosomal_protein_L17_129281..129431_POSITIVE