Origin IGS: nnnnnctgataaagataatgttacattagaaacaaaaactatcgtaagcttataatccagtttttgagattaagtttgcaaatggagttctttatcaaaatgggaaagtgattgatcaggatccc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gggatcctgatcaatcactttcccattttgataaagaactccatttgcaaacttaatctcaaaaactggattataagcttacgatagtttttgtttctaatgtaacattatctttatcagnnnnn Mask Tandem Repeat Region ================================================ nnnnnctgataaagataatgttacattagaaacaaaaactatcgtaagcttataatccagtttttgagattaagtttgcaaatggagttctttatcaaaatgggaaagtgattgatcaggatccc Find is-nt database================================================ Query_seq: SMT_1746:SMT_1747|SMT_1746:SMT_1747:glutamyl aminopeptidase:anthranilate synthase component I:->->:1726294..1726418 125 nnnnnctgataaagataatgttacattagaaacaaaaactatcgtaagcttataatccagtttttgagattaagtttgcaaatggagttctttatcaaaatgggaaagtgattgatcaggatccc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1746:SMT_1747|SMT_1746:SMT_1747:glutamyl aminopeptidase:anthranilate synthase component I:->->:1726294..1726418 125 nnnnnctgataaagataatgttacattagaaacaaaaactatcgtaagcttataatccagtttttgagattaagtttgcaaatggagttctttatcaaaatgggaaagtgattgatcaggatccc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1746:SMT_1747|SMT_1746:SMT_1747:glutamyl aminopeptidase:anthranilate synthase component I:->->:1726294..1726418 125 nnnnnctgataaagataatgttacattagaaacaaaaactatcgtaagcttataatccagtttttgagattaagtttgcaaatggagttctttatcaaaatgggaaagtgattgatcaggatccc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 nnnnnctgataaagataatgttacattagaaacaaaaactatcgtaagcttataatccagtttttgagattaagtttgcaaatggagttctttatcaaaatgggaaagtgattgatcaggatccc Predict ORF larger than 30AA ================================================ nnnnnctgataaagataatgttacattagaaacaaaaactatcgtaagcttataatccagtttttgagattaagtttgcaaatggagttctttatcaaaatgggaaagtgattgatcaggatccc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ nnnnnctgataaagataatgttacattagaaacaaaaactatcgtaagcttataatccagtttttgagattaagtttgcaaatggagttctttatcaaaatgggaaagtgattgatcaggatccc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1746:SMT_1747|SMT_1746:SMT_1747:glutamyl aminopeptidase:anthranilate synthase component I:->->:1726294..1726418 125 nnnnnctgataaagataatgttacattagaaacaaaaactatcgtaagcttataatccagtttttgagattaagtttgcaaatggagttctttatcaaaatgggaaagtgattgatcaggatccc Intra-Species Hit: Count: 1 Min: 6 Max: 125 Len: 120 Subject: smit_SMT_1746_SMT_1747|glutamyl aminopeptidase:anthranilate synthase component I|POSITIVE:POSITIVE|[1726294,1726418]|125 HSP 1 e-value: 3.0E-62 bit: 238.0 Len: 120 Query Start:6 Query End:125 Subject Strand: POSITIVE Subject Start: 6 Subject End: 125 .....ctgataaagataatgttacattagaaacaaaaactatcgtaagcttataatccagtttttgagattaagtttgcaaatggagttctttatcaaaatgggaaagtgattgatcaggatccc .....ctgataaagataatgttacattagaaacaaaaactatcgtaagcttataatccagtttttgagattaagtttgcaaatggagttctttatcaaaatgggaaagtgattgatcaggatccc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUGAUAAAGAUAAUGUUACAUUAGAAACAAAAACUAUCGUAAGCUUAUAAUCCAGUUUUUGAGAUUAAGUUUGCAAAUGGAGUUCUUUAUCAAAAUGGGAAAGUGAUUGAUCAGGAUCCC .(((((((((...((((........))))....((((.(((((((((...((........))...)))))))))..))))...)))))))))....((((...(((....)))...)))) (-25.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGGAUCCUGAUCAAUCACUUUCCCAUUUUGAUAAAGAACUCCAUUUGCAAACUUAAUCUCAAAAACUGGAUUAUAAGCUUACGAUAGUUUUUGUUUCUAAUGUAACAUUAUCUUUAUCAG ((((...(((....)))...))))....(((((((((.......(((((...(((....(((((((((...((......))...)))))))))....)))))))).....))))))))). (-23.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 255 117 71 76 40 smit:SMT_598|5end_K07273_lysozyme_514764..514994_NEGATIVE 242 118 73 178 124 smit:SMT_958|5end_hypothetical_protein_predicted_by_Glimmer/Critica_905052..905282_POSITIVE 233 80 34 220 169 smit:SMT_1685|5end_phenylalanyl-tRNA_synthetase_alpha_chain_1668845..1669075_NEGATIVE 231 80 39 75 33 smit:SMT_115|5end_hypothetical_protein_65521..65751_POSITIVE 228 47 7 206 166 smit:SMT_1043|5end_chaperonin_GroEL_985766..985996_NEGATIVE 227 88 41 45 2 smit:SMT_588|5end_hypothetical_protein_505477..505707_NEGATIVE 223 120 71 229 187 smit:SMT_1245|5end_SOS_responce_UmuC_protein_1205120..1205350_POSITIVE 221 74 35 56 4 smit:SMT_1008|5end_hypothetical_protein_948021..948251_NEGATIVE 220 115 83 160 120 smit:SMT_1430|5end_hypothetical_protein_1414857..1415087_POSITIVE 220 85 42 223 179 smit:SMT_179|5end_ribosomal_protein_S17_119392..119622_POSITIVE 218 109 62 81 35 smit:SMT_900|5end_5-methyltetrahydropteroyltriglutamate--_homocysteine_methyltransferase_850616..850846_NEGATIVE 218 116 76 217 175 smit:SMT_812|5end_acetyltransferase,_GNAT_family_721858..722088_NEGATIVE 217 58 14 61 14 smit:SMT_1487|5end_hypothetical_protein_1466361..1466591_POSITIVE 216 119 71 210 156 smit:SMT_1285|5end_ribosomal_subunit_interface_protein_1247704..1247934_POSITIVE 211 120 83 143 99 smit:SMT_2036|5end_spermidine_synthase_1947879..1948109_NEGATIVE 211 120 83 143 99 smit:SMT_1417|5end_spermidine_synthase_1401686..1401916_NEGATIVE 210 100 51 96 56 smit:SMT_2006|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1926472..1926702_POSITIVE 209 116 78 38 2 smit:SMT_500|5end_hypothetical_protein_432770..433000_POSITIVE 207 120 73 100 58 smit:SMT_2035|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1947167..1947397_POSITIVE 206 100 51 122 69 smit:SMT_463|5end_para-aminobenzoate_synthetase_component_I_/_4-amino-4-deoxychorismate_lyase_401926..402156_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 217 58 14 64 17 smit:SMT_1486|3end_hypothetical_protein_1466444..1466594_POSITIVE 217 67 21 93 39 smit:SMT_603|3end_hypothetical_protein_518532..518682_POSITIVE 216 119 71 130 76 smit:SMT_1284|3end_predicted_amidophosphoribosyltransferase_1247704..1247854_POSITIVE 213 120 77 129 87 smit:SMT_1244|3end_putative_repressor_protein_1205149..1205299_POSITIVE 212 116 73 122 79 smit:SMT_562|3end_ATP-dependent_RNA_helicase,_DEAD/DEAH_box_family_480809..480959_NEGATIVE 211 120 83 142 98 smit:SMT_2037|3end_lysine_decarboxylase_1947880..1948030_NEGATIVE 211 120 83 142 98 smit:SMT_1896|3end_lysine_decarboxylase_1848073..1848223_NEGATIVE 211 120 83 142 98 smit:SMT_1418|3end_lysine_decarboxylase_1401687..1401837_NEGATIVE 207 120 73 110 68 smit:SMT_2034|3end_hydrolase,_haloacid_dehalogenase-like_family_1947257..1947407_POSITIVE 207 108 70 145 104 smit:SMT_1429|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1414936..1415086_POSITIVE 205 120 71 148 91 smit:SMT_245|3end_hypothetical_protein_predicted_by_Glimmer/Critica_181863..182013_NEGATIVE 204 106 61 80 38 smit:SMT_363|3end_K02519_translation_initiation_factor_IF-2_300751..300901_POSITIVE 201 92 55 145 98 smit:SMT_1227|3end_site-specific_recombinase,_phage_integrase_family_1189311..1189461_NEGATIVE 199 74 31 50 7 smit:SMT_1243|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1204469..1204619_POSITIVE 197 80 33 53 1 smit:SMT_1075|3end_hypothetical_protein_1013870..1014020_POSITIVE 194 99 52 69 26 smit:SMT_2074|3end_dTDP-glucose_4,6-dehydratase_1977802..1977952_POSITIVE 192 75 34 69 36 smit:SMT_464|3end_ribonuclease_Z_403303..403453_POSITIVE 192 75 34 69 36 smit:SMT_416|3end_ribonuclease_Z_360432..360582_POSITIVE 192 100 51 81 50 smit:SMT_405|3end_zinc_metalloprotease_ZmpB,_truncated_339938..340088_POSITIVE 188 119 72 86 48 smit:SMT_2094|3end_serine_protease_1989243..1989393_NEGATIVE