Origin IGS: gattatcttaaaaacaggagtgttatatgaaaagaactatagaaattattagaacatggtcagctgataaaattgaattaatttttcttaaaagctggagctactttgctagctcatttagctagttkgtttatataaattagtttgtaattttttataggagtttttt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaaaaactcctataaaaaattacaaactaatttatataaacmaactagctaaatgagctagcaaagtagctccagcttttaagaaaaattaattcaattttatcagctgaccatgttctaataatttctatagttcttttcatataacactcctgtttttaagataatc Mask Tandem Repeat Region ================================================ gattatcttaaaaacaggagtgttatatgaaaagaactatagaaattattagaacatggtcagctgataaaattgaattaatttttcttaaaagctggagctactttgctagctcatttagctagttkgtttatataaattagtttgtaattttttataggagtttttt Find is-nt database================================================ Query_seq: SMT_1723:SMT_1724|SMT_1723:SMT_1724:hypothetical protein:hypothetical protein predicted by Glimmer/Critica:->->:1709785..1709953 169 gattatcttaaaaacaggagtgttatatgaaaagaactatagaaattattagaacatggtcagctgataaaattgaattaatttttcttaaaagctggagctactttgctagctcatttagctagttkgtttatataaattagtttgtaattttttataggagtttttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1723:SMT_1724|SMT_1723:SMT_1724:hypothetical protein:hypothetical protein predicted by Glimmer/Critica:->->:1709785..1709953 169 gattatcttaaaaacaggagtgttatatgaaaagaactatagaaattattagaacatggtcagctgataaaattgaattaatttttcttaaaagctggagctactttgctagctcatttagctagttkgtttatataaattagtttgtaattttttataggagtttttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1723:SMT_1724|SMT_1723:SMT_1724:hypothetical protein:hypothetical protein predicted by Glimmer/Critica:->->:1709785..1709953 169 gattatcttaaaaacaggagtgttatatgaaaagaactatagaaattattagaacatggtcagctgataaaattgaattaatttttcttaaaagctggagctactttgctagctcatttagctagttkgtttatataaattagtttgtaattttttataggagtttttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gattatcttaaaaacaggagtgttatatgaaaagaactatagaaattattagaacatggtcagctgataaaattgaattaatttttcttaaaagctggagctactttgctagctcatttagctagttkgtttatataaattagtttgtaattttttataggagtttttt Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 27 End: 122 .......................... M K R T I E I I R T W S A D K I E L I F L K S W S Y F A S S F S ............................................... gattatcttaaaaacaggagtgttatatgaaaagaactatagaaattattagaacatggtcagctgataaaattgaattaatttttcttaaaagctggagctactttgctagctcatttagctagttkgtttatataaattagtttgtaattttttataggagtttttt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gattatcttaaaaacaggagtgttatatgaaaagaactatagaaattattagaacatggtcagctgataaaattgaattaatttttcttaaaagctggagctactttgctagctcatttagctagttkgtttatataaattagtttgtaattttttataggagtttttt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1723:SMT_1724|SMT_1723:SMT_1724:hypothetical protein:hypothetical protein predicted by Glimmer/Critica:->->:1709785..1709953 169 gattatcttaaaaacaggagtgttatatgaaaagaactatagaaattattagaacatggtcagctgataaaattgaattaatttttcttaaaagctggagctactttgctagctcatttagctagttkgtttatataaattagtttgtaattttttataggagtttttt Intra-Species Hit: Count: 1 Min: 1 Max: 169 Len: 169 Subject: smit_SMT_1723_SMT_1724|hypothetical protein:hypothetical protein predicted by Glimmer/Critica|POSITIVE:POSITIVE|[1709785,1709953]|169 HSP 1 e-value: 2.0E-90 bit: 332.0 Len: 169 Query Start:1 Query End:169 Subject Strand: POSITIVE Subject Start: 1 Subject End: 169 gattatcttaaaaacaggagtgttatatgaaaagaactatagaaattattagaacatggtcagctgataaaattgaattaatttttcttaaaagctggagctactttgctagctcatttagctagttkgtttatataaattagtttgtaattttttataggagtttttt gattatcttaaaaacaggagtgttatatgaaaagaactatagaaattattagaacatggtcagctgataaaattgaattaatttttcttaaaagctggagctactttgctagctcatttagctagttkgtttatataaattagtttgtaattttttataggagtttttt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUUAUCUUAAAAACAGGAGUGUUAUAUGAAAAGAACUAUAGAAAUUAUUAGAACAUGGUCAGCUGAUAAAAUUGAAUUAAUUUUUCUUAAAAGCUGGAGCUACUUUGCUAGCUCAUUUAGCUAGUUKGUUUAUAUAAAUUAGUUUGUAAUUUUUUAUAGGAGUUUUUU .....((((......)))).........((((((..(((((((((......((((.(((((((((...(((((((...))))))).......)))))..))))....(((((((.....)))))))................)))).....)))))))))...)))))) (-27.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAAACUCCUAUAAAAAAUUACAAACUAAUUUAUAUAAACMAACUAGCUAAAUGAGCUAGCAAAGUAGCUCCAGCUUUUAAGAAAAAUUAAUUCAAUUUUAUCAGCUGACCAUGUUCUAAUAAUUUCUAUAGUUCUUUUCAUAUAACACUCCUGUUUUUAAGAUAAUC .((((((..((((...((((((.....))))))...........((((((.....))))))....))))...(((((..(((((.............)))))..))))).............................................))))))......... (-18.42) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 345 127 83 69 26 smit:SMT_1555|5end_hypothetical_protein_1541014..1541244_NEGATIVE 284 116 72 190 134 smit:SMT_1668|5end_K+_transport_system,_NAD-binding_component_1658288..1658518_NEGATIVE 271 120 75 171 125 smit:SMT_141|5end_K03495_glucose_inhibited_division_protein_A_91408..91638_POSITIVE 238 131 94 227 183 smit:SMT_1254|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1220882..1221112_NEGATIVE 236 117 74 190 149 smit:SMT_1590|5end_DNA-entry_nuclease_1578565..1578795_POSITIVE 235 115 71 167 122 smit:SMT_845|5end_hypothetical_protein_791868..792098_NEGATIVE 232 127 86 102 64 smit:SMT_1116|5end_K02244_competence_protein_ComGB_1053841..1054071_NEGATIVE 230 125 85 202 170 smit:SMT_1751|5end_indole-3-glycerol_phosphate_synthase_1729012..1729242_POSITIVE 230 126 91 68 26 smit:SMT_796|5end_K03734_thiamine_biosynthesis_lipoprotein_704211..704441_POSITIVE 228 115 84 142 110 smit:SMT_1576|5end_ribose_ABC_transporter_protein_RbsD_1568950..1569180_POSITIVE 227 124 95 225 185 smit:SMT_1786|5end_phenylalanyl-tRNA_synthetase_beta_chain_1763540..1763770_NEGATIVE 226 125 95 163 137 smit:SMT_755|5end_shikimate_5-dehydrogenase_659917..660147_NEGATIVE 225 117 74 57 3 smit:SMT_1541|5end_isopentenyl-diphosphate_delta-isomerase_1526093..1526323_NEGATIVE 225 124 81 157 108 smit:SMT_1371|5end_hypothetical_protein_1350794..1351024_NEGATIVE 225 127 92 62 22 smit:SMT_749|5end_shikimate_kinase_653971..654201_NEGATIVE 225 124 96 61 27 smit:SMT_113|5end_hypothetical_protein_61425..61655_POSITIVE 224 113 72 62 17 smit:SMT_1120|5end_hypothetical_protein_1057063..1057293_NEGATIVE 220 127 93 53 17 smit:SMT_934|5end_pyridine_nucleotide-disulfide_oxidoreductase_885911..886141_POSITIVE 219 122 93 207 175 smit:SMT_1395|5end_K07025_putative_hydrolase_of_the_HAD_superfamily_1371310..1371540_POSITIVE 217 100 53 173 124 smit:SMT_271|5end_autoinducer-2_production_protein_211736..211966_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 241 136 94 131 78 smit:SMT_1255|3end_cell_division_protein_FtsH_1220987..1221137_NEGATIVE 240 149 103 53 2 smit:SMT_1789|3end_hypothetical_protein_1765511..1765661_POSITIVE 236 117 74 151 110 smit:SMT_1589|3end_hypothetical_protein_1578606..1578756_POSITIVE 227 124 95 97 57 smit:SMT_1787|3end_phosphoribosylformylglycinamidine_synthase_1763668..1763818_NEGATIVE 225 117 74 73 19 smit:SMT_1542|3end_phosphomevalonate_kinase_1526077..1526227_NEGATIVE 225 124 81 148 99 smit:SMT_1372|3end_hypothetical_protein_1350803..1350953_NEGATIVE 225 127 92 69 29 smit:SMT_750|3end_3-phosphoshikimate_1-carboxyvinyltransferase_653964..654114_NEGATIVE 219 127 86 125 90 smit:SMT_1945|3end_putative_dihydrolipoamide_S-acetyltransferase_1880646..1880796_POSITIVE 218 123 93 133 103 smit:SMT_739|3end_50S_ribosomal_protein_L7/L12_643274..643424_NEGATIVE 217 100 53 78 29 smit:SMT_272|3end_hypothetical_protein_211831..211981_NEGATIVE 216 110 63 123 72 smit:SMT_779|3end_ribonuclease_BN,_putative_687883..688033_NEGATIVE 216 123 81 139 84 smit:SMT_189|3end_50S_ribosomal_protein_L15_124352..124502_POSITIVE 214 127 86 126 89 smit:SMT_575|3end_pyruvate_kinase_494813..494963_POSITIVE 211 120 72 90 51 smit:SMT_2078|3end_acetyl_xylan_esterase,_putative_1979839..1979989_POSITIVE 211 126 90 118 71 smit:SMT_1123|3end_hypothetical_protein_1060229..1060379_POSITIVE 210 124 93 107 71 smit:SMT_1524|3end_peptidase_T_1505867..1506017_POSITIVE 210 131 91 67 24 smit:SMT_451|3end_thiamine-phosphate_pyrophosphorylase_389647..389797_POSITIVE 209 125 94 147 108 smit:SMT_1798|3end_exonuclease_RexA_1772818..1772968_NEGATIVE 207 114 93 42 20 smit:SMT_98|3end_hypothetical_protein_30272..30422_POSITIVE 206 127 89 72 42 smit:SMT_316|3end_acetyl-CoA_carboxylase_carboxyl_transferase_subunit_alpha_257391..257541_POSITIVE