Origin IGS: atagtttctctatttttgtgcttttgtattggttttcgttacaatttgtatctgaatttattgcatttcgctaatttctatgatatccttttgaaggaggtgtat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atacacctccttcaaaaggatatcatagaaattagcgaaatgcaataaattcagatacaaattgtaacgaaaaccaatacaaaagcacaaaaatagagaaactat Mask Tandem Repeat Region ================================================ atagtttctctatttttgtgcttttgtattggttttcgttacaatttgtatctgaatttattgcatttcgctaatttctatgatatccttttgaaggaggtgtat Find is-nt database================================================ Query_seq: SMT_1715:SMT_1716|SMT_1715:SMT_1716:MccC family protein:hypothetical protein:->->:1703349..1703453 105 atagtttctctatttttgtgcttttgtattggttttcgttacaatttgtatctgaatttattgcatttcgctaatttctatgatatccttttgaaggaggtgtat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1715:SMT_1716|SMT_1715:SMT_1716:MccC family protein:hypothetical protein:->->:1703349..1703453 105 atagtttctctatttttgtgcttttgtattggttttcgttacaatttgtatctgaatttattgcatttcgctaatttctatgatatccttttgaaggaggtgtat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1715:SMT_1716|SMT_1715:SMT_1716:MccC family protein:hypothetical protein:->->:1703349..1703453 105 atagtttctctatttttgtgcttttgtattggttttcgttacaatttgtatctgaatttattgcatttcgctaatttctatgatatccttttgaaggaggtgtat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atagtttctctatttttgtgcttttgtattggttttcgttacaatttgtatctgaatttattgcatttcgctaatttctatgatatccttttgaaggaggtgtat Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 1 End: 105 M V S L F L C F C I G F R Y N L Y L N L L H F A N F Y D I L L K E V Y atagtttctctatttttgtgcttttgtattggttttcgttacaatttgtatctgaatttattgcatttcgctaatttctatgatatccttttgaaggaggtgtat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atagtttctctatttttgtgcttttgtattggttttcgttacaatttgtatctgaatttattgcatttcgctaatttctatgatatccttttgaaggaggtgtat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1715:SMT_1716|SMT_1715:SMT_1716:MccC family protein:hypothetical protein:->->:1703349..1703453 105 atagtttctctatttttgtgcttttgtattggttttcgttacaatttgtatctgaatttattgcatttcgctaatttctatgatatccttttgaaggaggtgtat Intra-Species Hit: Count: 1 Min: 1 Max: 105 Len: 105 Subject: smit_SMT_1715_SMT_1716|MccC family protein:hypothetical protein|POSITIVE:POSITIVE|[1703349,1703453]|105 HSP 1 e-value: 3.0E-53 bit: 208.0 Len: 105 Query Start:1 Query End:105 Subject Strand: POSITIVE Subject Start: 1 Subject End: 105 atagtttctctatttttgtgcttttgtattggttttcgttacaatttgtatctgaatttattgcatttcgctaatttctatgatatccttttgaaggaggtgtat atagtttctctatttttgtgcttttgtattggttttcgttacaatttgtatctgaatttattgcatttcgctaatttctatgatatccttttgaaggaggtgtat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAGUUUCUCUAUUUUUGUGCUUUUGUAUUGGUUUUCGUUACAAUUUGUAUCUGAAUUUAUUGCAUUUCGCUAAUUUCUAUGAUAUCCUUUUGAAGGAGGUGUAU ...((......((((..((((..(((((.((.....)).)))))...))))..)))).....))....((((..((((..............))))..))))... (-12.34) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUACACCUCCUUCAAAAGGAUAUCAUAGAAAUUAGCGAAAUGCAAUAAAUUCAGAUACAAAUUGUAACGAAAACCAAUACAAAAGCACAAAAAUAGAGAAACUAU .......((((.....))))....((((......((.....))......(((.(.(((.....))).))))..............................)))) ( -5.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 251 100 51 109 66 smit:SMT_812|5end_acetyltransferase,_GNAT_family_721858..722088_NEGATIVE 237 82 42 143 101 smit:SMT_1589|5end_hypothetical_protein_1578332..1578562_POSITIVE 237 46 2 195 148 smit:SMT_143|5end_hypothetical_protein_95323..95553_NEGATIVE 236 97 60 220 184 smit:SMT_1027|5end_K02026_multiple_sugar_transport_system_permease_protein_963474..963704_NEGATIVE 227 37 1 53 12 smit:SMT_865|5end_50S_ribosomal_protein_L1_815711..815941_NEGATIVE 219 91 51 199 162 smit:SMT_895|5end_cysteinyl-tRNA_synthetase_843037..843267_NEGATIVE 218 43 2 176 136 smit:SMT_1487|5end_hypothetical_protein_1466361..1466591_POSITIVE 211 99 57 229 185 smit:SMT_909|5end_type_I_restriction_enzyme_M_protein_858827..859057_NEGATIVE 211 49 4 104 59 smit:SMT_168|5end_anaerobic_ribonucleoside-triphosphate_reductase_activating_protein_113670..113900_POSITIVE 210 100 51 221 170 smit:SMT_2174|5end_UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-_diaminopimelate--D-alanyl-D-alanine_ligase_2043987..2044217_POSITIVE 210 87 48 64 18 smit:SMT_877|5end_hypothetical_protein_824412..824642_NEGATIVE 209 40 9 215 184 smit:SMT_498|5end_hypothetical_protein_431751..431981_POSITIVE 208 40 19 56 35 smit:SMT_1940|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1876589..1876819_POSITIVE 208 38 4 204 166 smit:SMT_1939|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1876278..1876508_POSITIVE 207 80 38 206 161 smit:SMT_646|5end_K07284_sortase_A_551702..551932_NEGATIVE 204 80 36 106 67 smit:SMT_1074|5end_catabolite_control_protein_A_1013285..1013515_NEGATIVE 204 65 36 230 201 smit:SMT_816|5end_SWF/SNF_family_helicase_727440..727670_NEGATIVE 203 48 4 160 105 smit:SMT_1734|5end_serine/threonine_protein_phosphatase_1718558..1718788_POSITIVE 201 50 6 213 175 smit:SMT_231|5end_translation_elongation_factor_G_166061..166291_POSITIVE 199 92 61 39 16 smit:SMT_1250|5end_hypothetical_protein_1211345..1211575_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 251 100 51 100 57 smit:SMT_813|3end_acetyltransferase,_GNAT_family_721867..722017_NEGATIVE 236 54 12 126 81 smit:SMT_458|3end_hypothetical_protein_predicted_by_Glimmer/Critica_396729..396879_NEGATIVE 219 91 51 117 80 smit:SMT_896|3end_hypothetical_protein_843119..843269_NEGATIVE 215 85 47 121 85 smit:SMT_1268|3end_acetoin_reductase_1232655..1232805_NEGATIVE 211 49 4 111 66 smit:SMT_167|3end_acetyltransferase,_GNAT_family_113757..113907_POSITIVE 210 87 48 74 28 smit:SMT_878|3end_excinuclease_ABC,_subunit_C_824402..824552_NEGATIVE 208 38 4 76 38 smit:SMT_1938|3end_zinc_metalloprotease_ZmpB,_putative_1876230..1876380_POSITIVE 207 78 42 151 114 smit:SMT_1588|3end_UDP-N-acetylglucosamine_1-carboxyvinyltransferase_1578425..1578575_POSITIVE 207 37 1 149 112 smit:SMT_720|3end_transcriptional_regulator_626878..627028_POSITIVE 206 50 3 130 72 smit:SMT_1203|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1164221..1164371_NEGATIVE 204 65 36 137 108 smit:SMT_817|3end_aminotransferase_727533..727683_NEGATIVE 199 92 61 55 32 smit:SMT_1251|3end_hypothetical_protein_1211329..1211479_NEGATIVE 197 43 4 148 103 smit:SMT_1733|3end_DNA_repair_protein_RecN_1718636..1718786_POSITIVE 195 44 2 144 96 smit:SMT_560|3end_predicted_permease_479284..479434_POSITIVE 191 71 34 74 34 smit:SMT_954|3end_cation-transporting_ATPase,_E1-E2_family_901677..901827_NEGATIVE 190 90 42 138 86 smit:SMT_1584|3end_methyltransferase,_putative_1575332..1575482_POSITIVE 187 40 13 59 31 smit:SMT_1939|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1876453..1876603_POSITIVE 184 40 2 85 55 smit:SMT_1689|3end_PTS_enzyme,_maltose-and_glucose-specific,_factor_II_homologue_1673139..1673289_POSITIVE 183 71 37 139 103 smit:SMT_1530|3end_K07456_DNA_mismatch_repair_protein_MutS2_1513010..1513160_NEGATIVE 181 55 12 123 75 smit:SMT_695|3end_heat_shock_protein_HtpX_605984..606134_NEGATIVE