Origin IGS: ctttcttctataaaaatagttaaataaatgacctataaaaaaagaaaacagtttcattgtaaaaaaacaaaaaaacagcaacaaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaattacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctttttctccttttcgtctatttaagataataggctagctccacaagaaaaccgacgcggtgttgcgtggcaatgcaaccgagcgtgtcgcaacctcttgcatcaaagctaaggctgtaatttacagcaccataatagaataacacaaagcccctgcgattgcaagggatttgttgctgtttttttgtttttttacaatgaaactgttttctttttttataggtcatttatttaactatttttatagaagaaag Mask Tandem Repeat Region ================================================ ctttcttctataaaaatagttaaataaatgacctataaaaaaagaaaacagtttcattgtaaaaaaacaaaaaaacagcaacaaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaattacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Find is-nt database================================================ Query_seq: SMT_168:SMT_169|SMT_168:SMT_169:anaerobic ribonucleoside-triphosphate reductase activating protein:30S ribosomal protein S10:->->:114401..114654 254 ctttcttctataaaaatagttaaataaatgacctataaaaaaagaaaacagtttcattgtaaaaaaacaaaaaaacagcaacaaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaattacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_168:SMT_169|SMT_168:SMT_169:anaerobic ribonucleoside-triphosphate reductase activating protein:30S ribosomal protein S10:->->:114401..114654 254 ctttcttctataaaaatagttaaataaatgacctataaaaaaagaaaacagtttcattgtaaaaaaacaaaaaaacagcaacaaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaattacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_168:SMT_169|SMT_168:SMT_169:anaerobic ribonucleoside-triphosphate reductase activating protein:30S ribosomal protein S10:->->:114401..114654 254 ctttcttctataaaaatagttaaataaatgacctataaaaaaagaaaacagtttcattgtaaaaaaacaaaaaaacagcaacaaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaattacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctttcttctataaaaatagttaaataaatgacctataaaaaaagaaaacagtttcattgtaaaaaaacaaaaaaacagcaacaaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaattacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 35 End: 130 .................................. M K K E N S F I V K K Q K N S N K S L A I A G A L C Y S I M V L ............................................................................................................................ Protein_Len: 60 Strand: + Start: 28 End: 234 ........................... M T Y K K R K Q F H C K K T K K Q Q Q I P C N R R G F V L F Y Y G A V N Y S L S F D A R G C D T L G C I A T Q H R V G F .................... Protein_Len: 31 Strand: - Start: 23 End: 115 ...................... I F S R Y F F F V T E N Y F F L F V A V F G K C D C P S Q T M ........................................................................................................................................... Protein_Len: 32 Strand: - Start: 1 End: 96 K R R Y F Y N F L H G I F F S F L K M T F F C F F L L L D R A M .............................................................................................................................................................. ctttcttctataaaaatagttaaataaatgacctataaaaaaagaaaacagtttcattgtaaaaaaacaaaaaaacagcaacaaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaattacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 56 End: 85 .......................................................TTTGTTGCTGTTTTTTTGTTTTTTTACAAT......................................................................................................................................................................... PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 89 End: 118 ........................................................................................CTTGCAATCGCAGGGGCTTTGTGTTATTCT........................................................................................................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctttcttctataaaaatagttaaataaatgacctataaaaaaagaaaacagtttcattgtaaaaaaacaaaaaaacagcaacaaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaattacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 80 HP_score: -3.0 Tail_Score: -5.64274 Start: 44 End: 55 Full_Region: ttgtttttttacaat gaaa ctgt tttc tttttttataggtca ...........................................gaaactgttttc....................................................................................................................................................................................................... TransTerm Strand: + Conf: 74 HP_score: -8.0 Tail_Score: -4.01089 Start: 80 End: 111 Full_Region: AAACAAAAAAACAGC AACAAATCCCTTGC AATC GCAGGGGCTTTGTG TTATTCTATTATGGT ...............................................................................AACAAATCCCTTGCAATCGCAGGGGCTTTGTG............................................................................................................................................... TransTerm Strand: + Conf: 81 HP_score: -10.6 Tail_Score: -3.58527 Start: 82 End: 109 Full_Region: ACAAAAAAACAGCAA CAAATCCCTTGC AATC GCAGGGGCTTTG TGTTATTCTATTATG .................................................................................CAAATCCCTTGCAATCGCAGGGGCTTTG................................................................................................................................................. TransTerm Strand: - Conf: 75 HP_score: -8.4 Tail_Score: -3.73271 Start: 82 End: 109 Full_Region: cataatagaataaca caaagcccctgc gatt gcaagggatttg ttgctgtttttttgt .................................................................................caaagcccctgcgattgcaagggatttg................................................................................................................................................. Find igs database================================================ Query_seq: SMT_168:SMT_169|SMT_168:SMT_169:anaerobic ribonucleoside-triphosphate reductase activating protein:30S ribosomal protein S10:->->:114401..114654 254 ctttcttctataaaaatagttaaataaatgacctataaaaaaagaaaacagtttcattgtaaaaaaacaaaaaaacagcaacaaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaattacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Intra-Species Hit: Count: 1 Min: 76 Max: 254 Len: 179 Subject: smit_SMT_168_SMT_169|anaerobic ribonucleoside-triphosphate reductase activating protein:30S ribosomal protein S10|POSITIVE:POSITIVE|[114401,114654]|254 HSP 1 e-value: 5.0E-97 bit: 355.0 Len: 179 Query Start:76 Query End:254 Subject Strand: POSITIVE Subject Start: 76 Subject End: 254 ...........................................................................cagcaacaaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaattacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag ...........................................................................cagcaacaaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaattacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Inter-species Hit: Count: 22 Min: 76 Max: 254 Len: 179 Subject: NC_008533_SPD_0191_SPD_0192|hypothetical protein:ribosomal protein S10|POSITIVE:POSITIVE|[195285,195550]|266 HSP 1 e-value: 1.0E-94 bit: 347.0 Len: 179 Query Start:76 Query End:254 Subject Strand: POSITIVE Subject Start: 88 Subject End: 266 ...........................................................................cagcaacaaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaattacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag ...........................................................................cagcaacaaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaattacagccttagttttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Subject: NC_003028_SP_0207_SP_0208|hypothetical protein:30S ribosomal protein S10|POSITIVE:POSITIVE|[192776,193041]|266 HSP 1 e-value: 1.0E-94 bit: 347.0 Len: 179 Query Start:76 Query End:254 Subject Strand: POSITIVE Subject Start: 88 Subject End: 266 ...........................................................................cagcaacaaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaattacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag ...........................................................................cagcaacaaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaattacagccttagttttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Subject: NC_003098_spr0186_spr0187|hypothetical protein:30S ribosomal protein S10|POSITIVE:POSITIVE|[195285,195550]|266 HSP 1 e-value: 3.0E-92 bit: 339.0 Len: 179 Query Start:76 Query End:254 Subject Strand: POSITIVE Subject Start: 88 Subject End: 266 ...........................................................................cagcaacaaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaattacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag ...........................................................................cagcaacaaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaattacagccttatttttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Subject: NC_009009_SSA_0105_SSA_0106|Uridine kinase, putative:30S ribosomal protein S10, putative|POSITIVE:POSITIVE|[107540,107911]|372 HSP 1 e-value: 2.0E-47 bit: 190.0 Len: 173 Query Start:82 Query End:254 Subject Strand: POSITIVE Subject Start: 198 Subject End: 372 .................................................................................caaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaat-tacagcct-tagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaa-caccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag .................................................................................caaatcccttgcaatgactggttctttgtgttaagatactatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacct-gtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Subject: NC_006086_M6_Spy0091_M6_Spy0092|Na+ driven multidrug efflux pump:30S ribosomal protein S10|POSITIVE:POSITIVE|[104934,105131]|198 HSP 1 e-value: 3.0E-36 bit: 153.0 Len: 132 Query Start:110 Query End:241 Subject Strand: POSITIVE Subject Start: 56 Subject End: 187 .............................................................................................................tgttattctattatggtgctgtaaat-tacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacga............. .............................................................................................................tgttattatattatggtgctgtaaaaatacagctttagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaaca-ggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_008024_MGAS10750_Spy0043_MGAS10750_Spy0044|Na+ driven multidrug efflux pump:SSU ribosomal protein S10P|POSITIVE:POSITIVE|[65353,65551]|199 HSP 1 e-value: 8.0E-34 bit: 145.0 Len: 132 Query Start:110 Query End:241 Subject Strand: POSITIVE Subject Start: 57 Subject End: 188 .............................................................................................................tgttattctattatggtgctgtaaat-tacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacga............. .............................................................................................................tgttattatattgtggtgctgtaaaaatacagctttagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaaca-ggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_008023_MGAS2096_Spy0045_MGAS2096_Spy0046|Na+ driven multidrug efflux pump:SSU ribosomal protein S10P|POSITIVE:POSITIVE|[63854,64051]|198 HSP 1 e-value: 8.0E-34 bit: 145.0 Len: 132 Query Start:110 Query End:241 Subject Strand: POSITIVE Subject Start: 56 Subject End: 187 .............................................................................................................tgttattctattatggtgctgtaaat-tacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacga............. .............................................................................................................tgttattatattgtggtgctgtaaaaatacagctttagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaaca-ggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_008022_MGAS10270_Spy0044_MGAS10270_Spy0045|Na+ driven multidrug efflux pump:SSU ribosomal protein S10P|POSITIVE:POSITIVE|[64251,64448]|198 HSP 1 e-value: 8.0E-34 bit: 145.0 Len: 132 Query Start:110 Query End:241 Subject Strand: POSITIVE Subject Start: 56 Subject End: 187 .............................................................................................................tgttattctattatggtgctgtaaat-tacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacga............. .............................................................................................................tgttattatattgtggtgctgtaaaaatacagctttagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaaca-ggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_008021_MGAS9429_Spy0042_MGAS9429_Spy0043|Na+ driven multidrug efflux pump:SSU ribosomal protein S10P|POSITIVE:POSITIVE|[63879,64076]|198 HSP 1 e-value: 8.0E-34 bit: 145.0 Len: 132 Query Start:110 Query End:241 Subject Strand: POSITIVE Subject Start: 56 Subject End: 187 .............................................................................................................tgttattctattatggtgctgtaaat-tacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacga............. .............................................................................................................tgttattatattgtggtgctgtaaaaatacagctttagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaaca-ggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_007297_M5005_Spy_0042_M5005_Spy_0043|Na+ driven multidrug efflux pump:30S ribosomal protein S10|POSITIVE:POSITIVE|[64101,64300]|200 HSP 1 e-value: 8.0E-34 bit: 145.0 Len: 132 Query Start:110 Query End:241 Subject Strand: POSITIVE Subject Start: 58 Subject End: 189 .............................................................................................................tgttattctattatggtgctgtaaat-tacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacga............. .............................................................................................................tgttattatattgtggtgctgtaaaaatacagctttagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaaca-ggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_007296_M28_Spy0041_M28_Spy0042|Na+ driven multidrug efflux pump:30S ribosomal protein S10|POSITIVE:POSITIVE|[63557,63754]|198 HSP 1 e-value: 8.0E-34 bit: 145.0 Len: 132 Query Start:110 Query End:241 Subject Strand: POSITIVE Subject Start: 56 Subject End: 187 .............................................................................................................tgttattctattatggtgctgtaaat-tacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacga............. .............................................................................................................tgttattatattgtggtgctgtaaaaatacagctttagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaaca-ggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_004606_SPs0039_SPs0040|hypothetical protein:30S ribosomal protein S10|POSITIVE:POSITIVE|[58271,58468]|198 HSP 1 e-value: 8.0E-34 bit: 145.0 Len: 132 Query Start:110 Query End:241 Subject Strand: POSITIVE Subject Start: 56 Subject End: 187 .............................................................................................................tgttattctattatggtgctgtaaat-tacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacga............. .............................................................................................................tgttattatattgtggtgctgtaaaaatacagctttagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaaca-ggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_004070_SpyM3_0038_SpyM3_0039|putative multi antimicrobial extrusion (MATE) family transporter:30S ribosomal protein S10|POSITIVE:POSITIVE|[64272,64469]|198 HSP 1 e-value: 8.0E-34 bit: 145.0 Len: 132 Query Start:110 Query End:241 Subject Strand: POSITIVE Subject Start: 56 Subject End: 187 .............................................................................................................tgttattctattatggtgctgtaaat-tacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacga............. .............................................................................................................tgttattatattgtggtgctgtaaaaatacagctttagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaaca-ggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_003485_spyM18_0048_spyM18_0049|hypothetical protein:30S ribosomal protein S10|POSITIVE:POSITIVE|[64163,64360]|198 HSP 1 e-value: 8.0E-34 bit: 145.0 Len: 132 Query Start:110 Query End:241 Subject Strand: POSITIVE Subject Start: 56 Subject End: 187 .............................................................................................................tgttattctattatggtgctgtaaat-tacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacga............. .............................................................................................................tgttattatattgtggtgctgtaaaaatacagctttagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaaca-ggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_002737_SPy0045_SPy0047|hypothetical protein:30S ribosomal protein S10|POSITIVE:POSITIVE|[63512,63711]|200 HSP 1 e-value: 8.0E-34 bit: 145.0 Len: 132 Query Start:110 Query End:241 Subject Strand: POSITIVE Subject Start: 58 Subject End: 189 .............................................................................................................tgttattctattatggtgctgtaaat-tacagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacga............. .............................................................................................................tgttattatattgtggtgctgtaaaaatacagctttagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaaca-ggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_004350_SMU.2026c_SMU.2027|30S ribosomal protein S10:putative transcriptional regulator|NEGATIVE:POSITIVE|[1893131,1893647]|517 HSP 1 e-value: 3.0E-33 bit: 143.0 Len: 119 Query Start:123 Query End:241 Subject Strand: NEGATIVE Subject Start: 210 Subject End: 329 ..........................................................................................................................tggtgctgtaaattacagcctt-agctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacga............. ..........................................................................................................................tggtgctgtaaaaatcagcctttagctttgatacaagaggttgcgatacgctcggttgcattgccacgcaaaagcctgtcggttttcttgtggagctagcctattattttaaatagacga............. Subject: NC_007432_SAK_0089_SAK_0090|MATE efflux family protein:30S ribosomal protein S10|POSITIVE:POSITIVE|[75794,75997]|204 HSP 1 e-value: 5.0E-29 bit: 129.0 Len: 105 Query Start:137 Query End:241 Subject Strand: POSITIVE Subject Start: 90 Subject End: 193 ........................................................................................................................................acagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacga............. ........................................................................................................................................acagctttagctatgatgcaagaggttgcgacacgctaggttgcattgccacgcaac-tcgtgttggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_004368_gbs0056_gbs0057|hypothetical protein:30S ribosomal protein S10|POSITIVE:POSITIVE|[76299,76503]|205 HSP 1 e-value: 5.0E-29 bit: 129.0 Len: 105 Query Start:137 Query End:241 Subject Strand: POSITIVE Subject Start: 91 Subject End: 194 ........................................................................................................................................acagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacga............. ........................................................................................................................................acagctttagctatgatgcaagaggttgcgacacgctaggttgcattgccacgcaac-tcgtgttggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_008532_STER_1908_STER_1909|Ribosomal protein S10:Holliday junction resolvasome, helicase subunit|NEGATIVE:NEGATIVE|[1769490,1769732]|243 HSP 1 e-value: 3.0E-27 bit: 123.0 Len: 144 Query Start:110 Query End:253 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 144 .............................................................................................................tgttattctattatggtgctgtaaattacagc-cttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaa-aggagaaaaa. .............................................................................................................tgttattatattatggtgctgttaaa-acagtgcttagctatgatacaagaggttgcgacacgcctggttgcactgccacgcaat-ccgtgttggttttcttgaggagctagcctattatcttaaatagacgaaagaggagaaaaa. Subject: NC_006449_str1935_str1936|30S ribosomal protein S10:Holliday junction DNA helicase RuvB|NEGATIVE:NEGATIVE|[1711847,1712089]|243 HSP 1 e-value: 3.0E-27 bit: 123.0 Len: 144 Query Start:110 Query End:253 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 144 .............................................................................................................tgttattctattatggtgctgtaaattacagc-cttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaa-aggagaaaaa. .............................................................................................................tgttattatattatggtgctgttaaa-acagtgcttagctatgatacaagaggttgcgacacgcctggttgcactgccacgcaat-ccgtgttggttttcttgaggagctagcctattatcttaaatagacgaaagaggagaaaaa. Subject: NC_004116_SAG0056_SAG0057|MATE efflux family protein:30S ribosomal protein S10|POSITIVE:POSITIVE|[76083,76287]|205 HSP 1 e-value: 1.0E-26 bit: 121.0 Len: 105 Query Start:137 Query End:241 Subject Strand: POSITIVE Subject Start: 91 Subject End: 194 ........................................................................................................................................acagccttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacga............. ........................................................................................................................................acagctttagctatgatgcaagaggttgcgacacgctaggttgcattgccacgcgac-tcgtgttggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_006448_stu1935_stu1936|30S ribosomal protein S10:Holliday junction DNA helicase RuvB|NEGATIVE:NEGATIVE|[1711223,1711465]|243 HSP 1 e-value: 3.0E-24 bit: 113.0 Len: 112 Query Start:142 Query End:253 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 112 .............................................................................................................................................cttagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaacaccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaa-aggagaaaaa. .............................................................................................................................................cttagctatgatacaagaggttgcgacacgcctggttgcactgccacgcaat-ccgtgttggttttcttgaggagttagcctattatcttaaatagacgaaagaggagaaaaa. Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAGCAACAAAUCCCUUGCAAUCGCAGGGGCUUUGUGUUAUUCUAUUAUGGUGCUGUAAAUUACAGCCUUAGCUUUGAUGCAAGAGGUUGCGACACGCUCGGUUGCAUUGCCACGCAACACCGCGUCGGUUUUCUUGUGGAGCUAGCCUAUUAUCUUAAAUAGACGAAAAGGAGAAAAAG .((((.((((.(((((((....))))))).))))))))..((((((..((.((((((...))))))))(((((((...((((((((...((((.((....(((((........)))))..)).))))..))))))))))))))).............))))))................ (-55.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUUUUUCUCCUUUUCGUCUAUUUAAGAUAAUAGGCUAGCUCCACAAGAAAACCGACGCGGUGUUGCGUGGCAAUGCAACCGAGCGUGUCGCAACCUCUUGCAUCAAAGCUAAGGCUGUAAUUUACAGCACCAUAAUAGAAUAACACAAAGCCCCUGCGAUUGCAAGGGAUUUGUUGCUG ....((((.......(((((((......)))))))(((((...(((((....(((((((.((((((((....)))))))..).))))))).....)))))......)))))..((((((...))))))........))))....(((((.(((.(((....))).))).)))))..... (-50.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 378 105 63 229 190 smit:SMT_739|5end_50S_ribosomal_protein_L7/L12_643642..643872_NEGATIVE 362 128 85 112 55 smit:SMT_2052|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1961591..1961821_NEGATIVE 357 129 84 216 164 smit:SMT_1945|5end_putative_dihydrolipoamide_S-acetyltransferase_1879961..1880191_POSITIVE 344 47 2 231 192 smit:SMT_1176|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1128993..1129223_NEGATIVE 336 128 83 197 154 smit:SMT_1982|5end_conserved_hypothetical_protein_1913036..1913266_POSITIVE 335 126 86 231 200 smit:SMT_2060|5end_hypothetical_protein_1968627..1968857_POSITIVE 334 130 83 218 162 smit:SMT_325|5end_K02029_polar_amino_acid_transport_system_permease_protein_263023..263253_NEGATIVE 330 109 64 78 41 smit:SMT_1591|5end_DNA-entry_nuclease_1578873..1579103_POSITIVE 329 110 62 79 27 smit:SMT_1213|5end_fatty_acid/phospholipid_synthesis_protein_PlsX_1172655..1172885_NEGATIVE 327 126 81 75 13 smit:SMT_1453|5end_K07335_basic_membrane_protein_A_and_related_proteins_1437407..1437637_NEGATIVE 325 110 66 59 7 smit:SMT_1970|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1902023..1902253_POSITIVE 325 139 95 148 89 smit:SMT_1940|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1876589..1876819_POSITIVE 325 122 82 65 35 smit:SMT_1704|5end_D-alanine-D-alanine_ligase_1687431..1687661_POSITIVE 316 146 105 76 26 smit:SMT_452|5end_phosphomethylpyrimidine_kinase_390568..390798_NEGATIVE 314 128 83 185 146 smit:SMT_1691|5end_putative_deoxyribonuclease_1674978..1675208_POSITIVE 314 128 83 210 171 smit:SMT_1156|5end_K07485_transposase_1099938..1100168_POSITIVE 312 108 65 184 135 smit:SMT_156|5end_hypothetical_protein_102869..103099_POSITIVE 310 140 91 63 3 smit:SMT_837|5end_cation_transport_ATPase_782667..782897_NEGATIVE 309 130 81 163 116 smit:SMT_1931|5end_23S_rRNA_methyltransferase_1870978..1871208_NEGATIVE 309 130 85 84 24 smit:SMT_1634|5end_2,3,4,5-tetrahydropyridine-2-carboxylate_N-succinyltransferase,_putative_1621074..1621304_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 360 105 65 51 9 smit:SMT_904|3end_hypothetical_protein_852294..852444_NEGATIVE 350 98 63 151 117 smit:SMT_740|3end_50S_ribosomal_protein_L10_643715..643865_NEGATIVE 330 109 64 105 68 smit:SMT_1590|3end_DNA-entry_nuclease_1578980..1579130_POSITIVE 329 110 62 82 30 smit:SMT_1214|3end_K03584_DNA_repair_protein_RecO_(recombination_protein_O)_1172652..1172802_NEGATIVE 323 128 83 81 33 smit:SMT_1484|3end_exonuclease_RexA_1464256..1464406_NEGATIVE 306 47 12 31 1 smit:SMT_1177|3end_immunoglobulin_A1_protease_1129193..1129343_NEGATIVE 304 110 66 137 89 smit:SMT_313|3end_3R-hydroxymyristoyl_ACP_dehydrase_254345..254495_POSITIVE 303 139 91 81 23 smit:SMT_1902|3end_proton-translocating_NADH-quinone_oxidoreductase,_chain_L_1852028..1852178_NEGATIVE 303 140 91 97 44 smit:SMT_850|3end_hypothetical_protein_796124..796274_NEGATIVE 302 137 93 63 6 smit:SMT_359|3end_transcription_termination-antitermination_factor_295686..295836_POSITIVE 297 144 102 126 86 smit:SMT_1977|3end_endopeptidase_O_1908454..1908604_POSITIVE 297 147 104 141 98 smit:SMT_1627|3end_hypothetical_protein_1616089..1616239_NEGATIVE 297 144 102 126 86 smit:SMT_1466|3end_endopeptidase_O_1447905..1448055_POSITIVE 296 130 84 142 83 smit:SMT_2111|3end_hypothetical_protein_predicted_by_Glimmer/Critica_2000036..2000186_POSITIVE 295 115 71 52 4 smit:SMT_1941|3end_hypothetical_protein_1877663..1877813_POSITIVE 293 124 86 151 122 smit:SMT_1953|3end_hypothetical_protein_1888107..1888257_POSITIVE 292 130 86 72 19 smit:SMT_1938|3end_zinc_metalloprotease_ZmpB,_putative_1876230..1876380_POSITIVE 292 110 63 72 8 smit:SMT_1823|3end_GTP-binding_protein_1796129..1796279_POSITIVE 292 138 94 148 115 smit:SMT_1778|3end_ATP-dependent_RNA_helicase_1753611..1753761_NEGATIVE 292 109 65 148 107 smit:SMT_1363|3end_50S_ribosomal_protein_L20_1343254..1343404_NEGATIVE