Origin IGS: ctagctacctaatttctatttttattcctagtcttgctaggtatgaggaaacctcatttcataaacttaattctaacataattttccgaaaaatcaaaaaatccaatacgagatagaaaaaaactgtaaggaaatccttacagtttgagt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 actcaaactgtaaggatttccttacagtttttttctatctcgtattggattttttgatttttcggaaaattatgttagaattaagtttatgaaatgaggtttcctcatacctagcaagactaggaataaaaatagaaattaggtagctag Mask Tandem Repeat Region ================================================ ctagctacctaatttctatttttattcctagtcttgctaggtatgaggaaacctcatttcataaacttaattctaacataattttccgaaaaatcaaaaaatccaatacgagatagaaaaaaactgtaaggaaatccttacagtttgagt Find is-nt database================================================ Query_seq: SMT_1655:SMT_1656|SMT_1655:SMT_1656:glucose-6-phosphate 1-dehydrogenase:glutamine ABC transporter ATP-binding protein:<-<-:1645112..1645261 150 ctagctacctaatttctatttttattcctagtcttgctaggtatgaggaaacctcatttcataaacttaattctaacataattttccgaaaaatcaaaaaatccaatacgagatagaaaaaaactgtaaggaaatccttacagtttgagt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1655:SMT_1656|SMT_1655:SMT_1656:glucose-6-phosphate 1-dehydrogenase:glutamine ABC transporter ATP-binding protein:<-<-:1645112..1645261 150 ctagctacctaatttctatttttattcctagtcttgctaggtatgaggaaacctcatttcataaacttaattctaacataattttccgaaaaatcaaaaaatccaatacgagatagaaaaaaactgtaaggaaatccttacagtttgagt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1655:SMT_1656|SMT_1655:SMT_1656:glucose-6-phosphate 1-dehydrogenase:glutamine ABC transporter ATP-binding protein:<-<-:1645112..1645261 150 ctagctacctaatttctatttttattcctagtcttgctaggtatgaggaaacctcatttcataaacttaattctaacataattttccgaaaaatcaaaaaatccaatacgagatagaaaaaaactgtaaggaaatccttacagtttgagt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctagctacctaatttctatttttattcctagtcttgctaggtatgaggaaacctcatttcataaacttaattctaacataattttccgaaaaatcaaaaaatccaatacgagatagaaaaaaactgtaaggaaatccttacagtttgagt Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 12 End: 113 ........... M S I F I P S L A R Y E E T S F H K L N S N I I F R K I K K S N T R ..................................... ctagctacctaatttctatttttattcctagtcttgctaggtatgaggaaacctcatttcataaacttaattctaacataattttccgaaaaatcaaaaaatccaatacgagatagaaaaaaactgtaaggaaatccttacagtttgagt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctagctacctaatttctatttttattcctagtcttgctaggtatgaggaaacctcatttcataaacttaattctaacataattttccgaaaaatcaaaaaatccaatacgagatagaaaaaaactgtaaggaaatccttacagtttgagt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1655:SMT_1656|SMT_1655:SMT_1656:glucose-6-phosphate 1-dehydrogenase:glutamine ABC transporter ATP-binding protein:<-<-:1645112..1645261 150 ctagctacctaatttctatttttattcctagtcttgctaggtatgaggaaacctcatttcataaacttaattctaacataattttccgaaaaatcaaaaaatccaatacgagatagaaaaaaactgtaaggaaatccttacagtttgagt Intra-Species Hit: Count: 1 Min: 1 Max: 150 Len: 150 Subject: smit_SMT_1655_SMT_1656|glucose-6-phosphate 1-dehydrogenase:glutamine ABC transporter ATP-binding protein|NEGATIVE:NEGATIVE|[1645112,1645261]|150 HSP 1 e-value: 2.0E-67 bit: 256.0 Len: 150 Query Start:1 Query End:150 Subject Strand: POSITIVE Subject Start: 1 Subject End: 150 ctagctacctaatttctatttttattcctagtcttgctaggtatgaggaaacctcatttcataaacttaattctaacataattttccgaaaaatcaaaaaatccaatacgagatagnnnnnnnctgtaaggaaatccttacagtttgagt ctagctacctaatttctatttttattcctagtcttgctaggtatgaggaaacctcatttcataaacttaattctaacataattttccgaaaaatcaaaaaatccaatacgagatagaaaaaaactgtaaggaaatccttacagtttgagt Inter-species Hit: Count: 3 Min: 1 Max: 150 Len: 150 Subject: NC_008533_SPD_1099_SPD_1100|amino acid ABC transporter, ATP-binding protein:glucose-6-phosphate 1-dehydrogenase|POSITIVE:POSITIVE|[1130095,1130242]|148 HSP 1 e-value: 3.0E-57 bit: 222.0 Len: 150 Query Start:1 Query End:150 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 148 ctagctacctaatttctatttttattcctagtcttgctaggtatgaggaaacctcatttcataaacttaattctaacataattttccgaaaaatcaaaaaatccaatacgagatagnnnnnnnctgtaaggaaatccttacagtttgagt ctagctacctaatttctatttttattcctagtcttgctaggtatgaggaaacctcatttcataaacttaattctaacataattttccgaaaaatcaaaaattccaatacgaggtag--aaaaactgcaaggaaatccttacagtttgagt Subject: NC_003098_spr1121_spr1122|ABC transporter ATP-binding protein - glutamine transport:Glucose-6-phosphate 1-dehydrogenase|POSITIVE:POSITIVE|[1122564,1122711]|148 HSP 1 e-value: 3.0E-57 bit: 222.0 Len: 150 Query Start:1 Query End:150 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 148 ctagctacctaatttctatttttattcctagtcttgctaggtatgaggaaacctcatttcataaacttaattctaacataattttccgaaaaatcaaaaaatccaatacgagatagnnnnnnnctgtaaggaaatccttacagtttgagt ctagctacctaatttctatttttattcctagtcttgctaggtatgaggaaacctcatttcataaacttaattctaacataattttccgaaaaatcaaaaattccaatacgaggtag--aaaaactgcaaggaaatccttacagtttgagt Subject: NC_003028_SP_1242_SP_1243|amino acid ABC transporter, ATP-binding protein:glucose-6-phosphate 1-dehydrogenase|POSITIVE:POSITIVE|[1174407,1174554]|148 HSP 1 e-value: 3.0E-57 bit: 222.0 Len: 150 Query Start:1 Query End:150 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 148 ctagctacctaatttctatttttattcctagtcttgctaggtatgaggaaacctcatttcataaacttaattctaacataattttccgaaaaatcaaaaaatccaatacgagatagnnnnnnnctgtaaggaaatccttacagtttgagt ctagctacctaatttctatttttattcctagtcttgctaggtatgaggaaacctcatttcataaacttaattctaacataattttccgaaaaatcaaaaattccaatacgaggtag--aaaaactgcaaggaaatccttacagtttgagt Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUAGCUACCUAAUUUCUAUUUUUAUUCCUAGUCUUGCUAGGUAUGAGGAAACCUCAUUUCAUAAACUUAAUUCUAACAUAAUUUUCCGAAAAAUCAAAAAAUCCAAUACGAGAUAGAAAAAAACUGUAAGGAAAUCCUUACAGUUUGAGU ............((((((((((....(((((.....))))).((((((...))))))....................................................)))))))))).(((((((((((....))))))))))).... (-33.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACUCAAACUGUAAGGAUUUCCUUACAGUUUUUUUCUAUCUCGUAUUGGAUUUUUUGAUUUUUCGGAAAAUUAUGUUAGAAUUAAGUUUAUGAAAUGAGGUUUCCUCAUACCUAGCAAGACUAGGAAUAAAAAUAGAAAUUAGGUAGCUAG ....(((((((((((....)))))))))))....((((((.((....((((((((((....)))))))))).........(((..(((((...((((((...)))))).(((((.....))))).)))))..)))..)).)))))).... (-37.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 819 50 1 141 92 smit:SMT_1655|5end_glucose-6-phosphate_1-dehydrogenase_1645021..1645251_NEGATIVE 283 148 113 130 90 smit:SMT_1198|5end_phosphoribosylaminoimidazole-succinocarboxamide_synthase_1159646..1159876_NEGATIVE 261 59 13 93 58 smit:SMT_139|5end_hypothetical_protein_90523..90753_POSITIVE 256 150 101 136 91 smit:SMT_1788|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1765029..1765259_NEGATIVE 256 150 101 136 91 smit:SMT_1197|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1158751..1158981_NEGATIVE 256 61 30 112 76 smit:SMT_613|5end_dihydrodipicolinate_synthase_523651..523881_POSITIVE 255 48 12 220 186 smit:SMT_1791|5end_hypothetical_protein_1765907..1766137_POSITIVE 252 49 4 169 121 smit:SMT_851|5end_conserved_hypothetical_protein,_translation_initiation_inhibitor_protein_797446..797676_NEGATIVE 250 60 11 66 12 smit:SMT_1328|5end_hypothetical_protein_1304468..1304698_NEGATIVE 244 56 16 68 25 smit:SMT_780|5end_peptidase_family_U32_689321..689551_NEGATIVE 241 48 1 163 115 smit:SMT_1202|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1164097..1164327_NEGATIVE 241 60 13 163 108 smit:SMT_271|5end_autoinducer-2_production_protein_211736..211966_NEGATIVE 240 47 1 219 165 smit:SMT_452|5end_phosphomethylpyrimidine_kinase_390568..390798_NEGATIVE 237 49 4 227 186 smit:SMT_609|5end_laminin-binding_surface_protein_521286..521516_POSITIVE 235 79 31 156 96 smit:SMT_941|5end_hypothetical_protein_893558..893788_NEGATIVE 230 46 1 80 25 smit:SMT_939|5end_phosphomethylpyrimidine_kinase_892025..892255_NEGATIVE 230 143 107 198 161 smit:SMT_855|5end_dihydrofolate_reductase_799998..800228_NEGATIVE 230 145 101 151 96 smit:SMT_506|5end_K07729_putative_transcriptional_regulator_437635..437865_NEGATIVE 230 145 101 151 96 smit:SMT_503|5end_K07729_putative_transcriptional_regulator_436204..436434_NEGATIVE 225 53 26 44 13 smit:SMT_403|5end_two-component_sensor_kinase_YesM_331580..331810_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 283 148 113 62 22 smit:SMT_1199|3end_lactococcin_A_ABC_transporter_permease_protein_1159714..1159864_NEGATIVE 256 150 101 50 5 smit:SMT_1198|3end_phosphoribosylaminoimidazole-succinocarboxamide_synthase_1158837..1158987_NEGATIVE 256 61 30 55 19 smit:SMT_612|3end_hypothetical_protein_predicted_by_Glimmer/Critica_523674..523824_POSITIVE 255 48 12 148 114 smit:SMT_1790|3end_hypothetical_protein_1765915..1766065_POSITIVE 250 60 11 56 2 smit:SMT_1329|3end_GTP-binding_protein_CgtA_1304478..1304628_NEGATIVE 244 150 109 66 24 smit:SMT_1290|3end_autolysin_1253753..1253903_POSITIVE 241 60 13 68 13 smit:SMT_272|3end_hypothetical_protein_211831..211981_NEGATIVE 237 49 4 48 7 smit:SMT_608|3end_laminin-binding_surface_protein_521187..521337_POSITIVE 230 46 1 90 35 smit:SMT_940|3end_tRNA_pseudouridine_synthase_A_892015..892165_NEGATIVE 230 143 107 66 29 smit:SMT_856|3end_peroxide_resistance_protein,non-heme_iron-containing_ferritin_800130..800280_NEGATIVE 230 145 101 148 93 smit:SMT_507|3end_hypothetical_protein_437638..437788_NEGATIVE 230 145 101 148 93 smit:SMT_504|3end_hypothetical_protein_436207..436357_NEGATIVE 225 53 26 47 16 smit:SMT_402|3end_two-component_response_regulator_YesN_331663..331813_POSITIVE 225 60 16 132 77 smit:SMT_348|3end_K07707_two-component_system,_AgrA_family,_response_regulator_AgrA_287282..287432_POSITIVE 223 59 12 119 79 smit:SMT_1596|3end_oligopeptide_transport_ATP-binding_protein_OppD_1587050..1587200_POSITIVE 222 55 11 119 75 smit:SMT_1259|3end_hypothetical_protein_1225578..1225728_POSITIVE 222 49 12 130 93 smit:SMT_377|3end_hypothetical_protein_315848..315998_POSITIVE 218 57 13 125 83 smit:SMT_1696|3end_hypothetical_protein_1678924..1679074_POSITIVE 216 60 28 147 112 smit:SMT_2026|3end_K03466_DNA_segregation_ATPase_FtsK/SpoIIIE,_S-DNA-T_family_1941905..1942055_NEGATIVE 213 46 4 150 110 smit:SMT_852|3end_K03978_GTP-binding_protein_797457..797607_NEGATIVE