Origin IGS: attttcaaaactggtccagagatagtagacttctgcccctgcttcatttaggacaccatcacgattatctagacgaagctnnnnnttaattaattaannnnngtccctgagttagatataatcttcctctctcttgactctgcaagtgaatcttttagtagctttattcgtctaaatatttgcgatttattcgc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gcgaataaatcgcaaatatttagacgaataaagctactaaaagattcacttgcagagtcaagagagaggaagattatatctaactcagggacnnnnnttaattaattaannnnnagcttcgtctagataatcgtgatggtgtcctaaatgaagcaggggcagaagtctactatctctggaccagttttgaaaat Mask Tandem Repeat Region ================================================ attttcaaaactggtccagagatagtagacttctgcccctgcttcatttaggacaccatcacgattatctagacgaagctnnnnnttaattaattaannnnngtccctgagttagatataatcttcctctctcttgactctgcaagtgaatcttttagtagctttattcgtctaaatatttgcgatttattcgc Find is-nt database================================================ Query_seq: SMT_1627:SMT_1628|SMT_1627:SMT_1628:hypothetical protein:hypothetical protein predicted by Glimmer/Critica:<-<-:1616506..1616699 194 attttcaaaactggtccagagatagtagacttctgcccctgcttcatttaggacaccatcacgattatctagacgaagctnnnnnttaattaattaannnnngtccctgagttagatataatcttcctctctcttgactctgcaagtgaatcttttagtagctttattcgtctaaatatttgcgatttattcgc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1627:SMT_1628|SMT_1627:SMT_1628:hypothetical protein:hypothetical protein predicted by Glimmer/Critica:<-<-:1616506..1616699 194 attttcaaaactggtccagagatagtagacttctgcccctgcttcatttaggacaccatcacgattatctagacgaagctnnnnnttaattaattaannnnngtccctgagttagatataatcttcctctctcttgactctgcaagtgaatcttttagtagctttattcgtctaaatatttgcgatttattcgc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1627:SMT_1628|SMT_1627:SMT_1628:hypothetical protein:hypothetical protein predicted by Glimmer/Critica:<-<-:1616506..1616699 194 attttcaaaactggtccagagatagtagacttctgcccctgcttcatttaggacaccatcacgattatctagacgaagctnnnnnttaattaattaannnnngtccctgagttagatataatcttcctctctcttgactctgcaagtgaatcttttagtagctttattcgtctaaatatttgcgatttattcgc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attttcaaaactggtccagagatagtagacttctgcccctgcttcatttaggacaccatcacgattatctagacgaagctnnnnnttaattaattaannnnngtccctgagttagatataatcttcctctctcttgactctgcaagtgaatcttttagtagctttattcgtctaaatatttgcgatttattcgc Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: - Start: 1 End: 90 N E F S T W L Y Y V E A G A E N L V G D R N D L R L X X X M ........................................................................................................ attttcaaaactggtccagagatagtagacttctgcccctgcttcatttaggacaccatcacgattatctagacgaagctnnnnnttaattaattaannnnngtccctgagttagatataatcttcctctctcttgactctgcaagtgaatcttttagtagctttattcgtctaaatatttgcgatttattcgc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attttcaaaactggtccagagatagtagacttctgcccctgcttcatttaggacaccatcacgattatctagacgaagctnnnnnttaattaattaannnnngtccctgagttagatataatcttcctctctcttgactctgcaagtgaatcttttagtagctttattcgtctaaatatttgcgatttattcgc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1627:SMT_1628|SMT_1627:SMT_1628:hypothetical protein:hypothetical protein predicted by Glimmer/Critica:<-<-:1616506..1616699 194 attttcaaaactggtccagagatagtagacttctgcccctgcttcatttaggacaccatcacgattatctagacgaagctnnnnnttaattaattaannnnngtccctgagttagatataatcttcctctctcttgactctgcaagtgaatcttttagtagctttattcgtctaaatatttgcgatttattcgc Intra-Species Hit: Count: 1 Min: 1 Max: 194 Len: 194 Subject: smit_SMT_1627_SMT_1628|hypothetical protein:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:NEGATIVE|[1616506,1616699]|194 HSP 1 e-value: 3.0E-88 bit: 325.0 Len: 194 Query Start:1 Query End:194 Subject Strand: POSITIVE Subject Start: 1 Subject End: 194 attttcaaaactggtccagagatagtagacttctgcccctgcttcatttaggacaccatcacgattatctagacgaagctnnnnnttaattaattaannnnngtccctgagttagatataatcttcctctctcttgactctgcaagtgaatcttttagtagctttattcgtctaaatatttgcgatttattcgc attttcaaaactggtccagagatagtagacttctgcccctgcttcatttaggacaccatcacgattatctagacgaagctnnnnnttaattaattaannnnngtccctgagttagatataatcttcctctctcttgactctgcaagtgaatcttttagtagctttattcgtctaaatatttgcgatttattcgc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUUCAAAACUGGUCCAGAGAUAGUAGACUUCUGCCCCUGCUUCAUUUAGGACACCAUCACGAUUAUCUAGACGAAGCUNNNNNUUAAUUAAUUAANNNNNGUCCCUGAGUUAGAUAUAAUCUUCCUCUCUCUUGACUCUGCAAGUGAAUCUUUUAGUAGCUUUAUUCGUCUAAAUAUUUGCGAUUUAUUCGC .............(((((((...(((((..........)))))...))).))))...(((.(((.(((.(((((((((((............................(((..((((...))))..)))((.((((......)))).))..........))))....))))))).))).))).)))........ (-35.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCGAAUAAAUCGCAAAUAUUUAGACGAAUAAAGCUACUAAAAGAUUCACUUGCAGAGUCAAGAGAGAGGAAGAUUAUAUCUAACUCAGGGACNNNNNUUAAUUAAUUAANNNNNAGCUUCGUCUAGAUAAUCGUGAUGGUGUCCUAAAUGAAGCAGGGGCAGAAGUCUACUAUCUCUGGACCAGUUUUGAAAAU ..((....(((((...(((((((((((....((((.......(((((.......)))))......(((..((((...))))..)))............................)))))))))))))))...)))))....))......(((((.((..((((((....))...))))..)).)))))...... (-42.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 992 60 11 151 102 smit:SMT_1627|5end_hypothetical_protein_1616415..1616645_NEGATIVE 330 60 11 61 4 smit:SMT_1793|5end_inosine-5'-monophosphate_dehydrogenase_1767985..1768215_POSITIVE 330 58 11 189 141 smit:SMT_889|5end_K02029_polar_amino_acid_transport_system_permease_protein_836266..836496_POSITIVE 324 79 33 43 5 smit:SMT_546|5end_DNA_topoisomerase_IV_subunit_A_461377..461607_POSITIVE 320 53 12 185 135 smit:SMT_1933|5end_K02006_cobalt_transport_system_ATP-binding_protein_1872222..1872452_NEGATIVE 310 76 34 197 149 smit:SMT_1438|5end_hypothetical_protein_1422877..1423107_NEGATIVE 306 59 20 48 1 smit:SMT_1076|5end_2-amino-4-hydroxy-6-_hydroxymethyldihydropteridine_pyrophosphokinase_/_dihydroneopterin_aldolase_1014813..1015043_NEGATIVE 303 49 6 72 29 smit:SMT_1170|5end_acetyltransferase,_CysE/LacA/LpxA/NodL_family_1118126..1118356_NEGATIVE 298 57 12 204 150 smit:SMT_1543|5end_diphosphomevalonate_decarboxylase_1528024..1528254_NEGATIVE 297 70 29 146 99 smit:SMT_311|5end_3-oxoacyl-_252123..252353_POSITIVE 296 50 19 154 116 smit:SMT_1307|5end_glycerol_uptake_facilitator_protein_1271664..1271894_POSITIVE 295 60 15 193 141 smit:SMT_109|5end_beta-galactosidase_53925..54155_POSITIVE 287 50 12 158 113 smit:SMT_1414|5end_hypothetical_protein_1398446..1398676_NEGATIVE 280 80 32 221 150 smit:SMT_976|5end_hypothetical_protein_925183..925413_NEGATIVE 279 47 9 91 55 smit:SMT_1915|5end_pyridoxine_biosynthesis_protein_1860329..1860559_POSITIVE 279 66 25 138 93 smit:SMT_1112|5end_K02248_competence_protein_ComGF_1051848..1052078_NEGATIVE 278 49 4 223 169 smit:SMT_1198|5end_phosphoribosylaminoimidazole-succinocarboxamide_synthase_1159646..1159876_NEGATIVE 278 63 22 48 12 smit:SMT_1118|5end_hypothetical_protein_1055150..1055380_NEGATIVE 274 75 31 142 93 smit:SMT_893|5end_leucine_rich_protein_841311..841541_NEGATIVE 272 70 30 220 171 smit:SMT_877|5end_hypothetical_protein_824412..824642_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 297 70 29 124 77 smit:SMT_310|3end_3-oxoacyl-_252181..252331_POSITIVE 296 50 19 83 45 smit:SMT_1306|3end_alpha-glycerophosphate_oxidase_1271673..1271823_POSITIVE 295 60 15 61 9 smit:SMT_108|3end_hypothetical_protein_53873..54023_POSITIVE 274 75 31 138 89 smit:SMT_894|3end_hypothetical_protein_841315..841465_NEGATIVE 271 150 104 130 88 smit:SMT_1006|3end_6-pyruvoyl_tetrahydrobiopterin_synthase,_putative_947122..947272_POSITIVE 266 44 18 40 12 smit:SMT_394|3end_K07729_putative_transcriptional_regulator_324549..324699_POSITIVE 265 172 132 69 19 smit:SMT_1881|3end_hypothetical_protein_1837781..1837931_POSITIVE 261 79 32 151 81 smit:SMT_977|3end_conserved_hypothetical_protein,_Rrf2_family_protein_925252..925402_NEGATIVE 256 38 9 84 52 smit:SMT_1291|3end_hypothetical_protein_1254296..1254446_POSITIVE 255 149 107 41 13 smit:SMT_712|3end_alcohol_dehydrogenase,_zinc-containing_620187..620337_NEGATIVE 255 149 107 41 13 smit:SMT_682|3end_hypothetical_protein_590838..590988_NEGATIVE 254 38 4 145 101 smit:SMT_1199|3end_lactococcin_A_ABC_transporter_permease_protein_1159714..1159864_NEGATIVE 253 143 108 105 68 smit:SMT_2175|3end_hypothetical_protein_2045212..2045362_NEGATIVE 253 143 108 105 68 smit:SMT_2169|3end_hypothetical_protein_2041383..2041533_NEGATIVE 253 143 108 105 68 smit:SMT_2151|3end_hypothetical_protein_2025026..2025176_NEGATIVE 253 143 108 105 68 smit:SMT_2148|3end_hypothetical_protein_2023141..2023291_NEGATIVE 253 143 108 105 68 smit:SMT_2147|3end_hypothetical_protein_2022401..2022551_NEGATIVE 253 143 108 105 68 smit:SMT_2144|3end_hypothetical_protein_2020647..2020797_NEGATIVE 253 143 108 105 68 smit:SMT_971|3end_hypothetical_protein_917916..918066_NEGATIVE 252 70 25 54 10 smit:SMT_1444|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1427887..1428037_NEGATIVE