Origin IGS: gctccagtcacggctgaataatcgtttaccaaagttgaagagaataccgatggnnnnnttaattaattaannnnnagatgatattcgtgccttcctaccacgctctgtccgtaatgtagaaaatgcagaagcacgtttgcgtgcagctctataaacatcaaggctgggagcaactcccagtcttattttaatttcaaaaagaaagaagaaagaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttctttcttctttctttttgaaattaaaataagactgggagttgctcccagccttgatgtttatagagctgcacgcaaacgtgcttctgcattttctacattacggacagagcgtggtaggaaggcacgaatatcatctnnnnnttaattaattaannnnnccatcggtattctcttcaactttggtaaacgattattcagccgtgactggagc Mask Tandem Repeat Region ================================================ gctccagtcacggctgaataatcgtttaccaaagttgaagagaataccgatggnnnnnttaattaattaannnnnagatgatattcgtgccttcctaccacgctctgtccgtaatgtagaaaatgcagaagcacgtttgcgtgcagctctataaacatcaaggctgggagcaactcccagtcttattttaatttcaaaaagaaagaagaaagaa Find is-nt database================================================ Query_seq: SMT_155:SMT_156|SMT_155:SMT_156:hypothetical protein:hypothetical protein:<-->:102795..103008 214 gctccagtcacggctgaataatcgtttaccaaagttgaagagaataccgatggnnnnnttaattaattaannnnnagatgatattcgtgccttcctaccacgctctgtccgtaatgtagaaaatgcagaagcacgtttgcgtgcagctctataaacatcaaggctgggagcaactcccagtcttattttaatttcaaaaagaaagaagaaagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_155:SMT_156|SMT_155:SMT_156:hypothetical protein:hypothetical protein:<-->:102795..103008 214 gctccagtcacggctgaataatcgtttaccaaagttgaagagaataccgatggnnnnnttaattaattaannnnnagatgatattcgtgccttcctaccacgctctgtccgtaatgtagaaaatgcagaagcacgtttgcgtgcagctctataaacatcaaggctgggagcaactcccagtcttattttaatttcaaaaagaaagaagaaagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_155:SMT_156|SMT_155:SMT_156:hypothetical protein:hypothetical protein:<-->:102795..103008 214 gctccagtcacggctgaataatcgtttaccaaagttgaagagaataccgatggnnnnnttaattaattaannnnnagatgatattcgtgccttcctaccacgctctgtccgtaatgtagaaaatgcagaagcacgtttgcgtgcagctctataaacatcaaggctgggagcaactcccagtcttattttaatttcaaaaagaaagaagaaagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gctccagtcacggctgaataatcgtttaccaaagttgaagagaataccgatggnnnnnttaattaattaannnnnagatgatattcgtgccttcctaccacgctctgtccgtaatgtagaaaatgcagaagcacgtttgcgtgcagctctataaacatcaaggctgggagcaactcccagtcttattttaatttcaaaaagaaagaagaaagaa Predict ORF larger than 30AA ================================================ Protein_Len: 41 Strand: - Start: 70 End: 192 ..................................................................... X X S S I R A K R G R E T R L T S F A S A R K R A A R Y V D L S P A V G L R I K M ...................... Protein_Len: 36 Strand: - Start: 66 End: 173 ................................................................. N X X L H Y E H R G V V S Q G Y H L F H L L V N A H L E I F M L A P L M ......................................... gctccagtcacggctgaataatcgtttaccaaagttgaagagaataccgatggnnnnnttaattaattaannnnnagatgatattcgtgccttcctaccacgctctgtccgtaatgtagaaaatgcagaagcacgtttgcgtgcagctctataaacatcaaggctgggagcaactcccagtcttattttaatttcaaaaagaaagaagaaagaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gctccagtcacggctgaataatcgtttaccaaagttgaagagaataccgatggnnnnnttaattaattaannnnnagatgatattcgtgccttcctaccacgctctgtccgtaatgtagaaaatgcagaagcacgtttgcgtgcagctctataaacatcaaggctgggagcaactcccagtcttattttaatttcaaaaagaaagaagaaagaa Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -11.6 Tail_Score: -4.54727 Start: 159 End: 185 Full_Region: CAGCTCTATAAACAT CAAGGCTGGGAG CAA CTCCCAGTCTTA TTTTAATTTCAAAAA ..............................................................................................................................................................CAAGGCTGGGAGCAACTCCCAGTCTTA............................. TransTerm Strand: + Conf: 100 HP_score: -13.3 Tail_Score: -4.38472 Start: 162 End: 182 Full_Region: CTCTATAAACATCAA GGCTGGGAG CAA CTCCCAGTC TTATTTTAATTTCAA .................................................................................................................................................................GGCTGGGAGCAACTCCCAGTC................................ Find igs database================================================ Query_seq: SMT_155:SMT_156|SMT_155:SMT_156:hypothetical protein:hypothetical protein:<-->:102795..103008 214 gctccagtcacggctgaataatcgtttaccaaagttgaagagaataccgatggnnnnnttaattaattaannnnnagatgatattcgtgccttcctaccacgctctgtccgtaatgtagaaaatgcagaagcacgtttgcgtgcagctctataaacatcaaggctgggagcaactcccagtcttattttaatttcaaaaagaaagaagaaagaa Intra-Species Hit: Count: 1 Min: 1 Max: 214 Len: 214 Subject: smit_SMT_155_SMT_156|hypothetical protein:hypothetical protein|NEGATIVE:POSITIVE|[102795,103008]|214 HSP 1 e-value: 2.0E-96 bit: 353.0 Len: 214 Query Start:1 Query End:214 Subject Strand: POSITIVE Subject Start: 1 Subject End: 214 gctccagtcacggctgaataatcgtttaccaaagttgaagagaataccgatnnnnnnnttaattaattaannnnnagatgatattcgtgccttcctaccacgctctgtccgtaatgtagaaaatgcagaagcacgtttgcgtgcagctctataaacatcaaggctgggagcaactcccagtcttattttaatttcaaaaagaaagaagaaagaa gctccagtcacggctgaataatcgtttaccaaagttgaagagaataccgatggnnnnnttaattaattaannnnnagatgatattcgtgccttcctaccacgctctgtccgtaatgtagaaaatgcagaagcacgtttgcgtgcagctctataaacatcaaggctgggagcaactcccagtcttattttaatttcaaaaagaaagaagaaagaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCUCCAGUCACGGCUGAAUAAUCGUUUACCAAAGUUGAAGAGAAUACCGAUGGNNNNNUUAAUUAAUUAANNNNNAGAUGAUAUUCGUGCCUUCCUACCACGCUCUGUCCGUAAUGUAGAAAAUGCAGAAGCACGUUUGCGUGCAGCUCUAUAAACAUCAAGGCUGGGAGCAACUCCCAGUCUUAUUUUAAUUUCAAAAAGAAAGAAGAAAGAA ((.........(((((((((.((((((.....((((((..((....((...)).....))..)))))).......)))))))))))).)))...((((.(((.......)))...)))).....))....(((((....)))))...............(((((((((((...))))))))))).((((..((((.....))))..)))).... (-44.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCUUUCUUCUUUCUUUUUGAAAUUAAAAUAAGACUGGGAGUUGCUCCCAGCCUUGAUGUUUAUAGAGCUGCACGCAAACGUGCUUCUGCAUUUUCUACAUUACGGACAGAGCGUGGUAGGAAGGCACGAAUAUCAUCUNNNNNUUAAUUAAUUAANNNNNCCAUCGGUAUUCUCUUCAACUUUGGUAAACGAUUAUUCAGCCGUGACUGGAGC ......((((..((...(((((.......((((.(((((((...))))))).))))..((((((((((..(((((....)))))...(((.((((((((..((((.......))))))))))))))).(((((((.........((((....))))..........))))))).......)))).)))))).....)))))....))..)))). (-47.81) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 960 140 91 231 182 smit:SMT_155|5end_hypothetical_protein_102704..102934_NEGATIVE 333 169 122 198 154 smit:SMT_2023|5end_tryptophan_synthase,_alpha_subunit_1939315..1939545_POSITIVE 330 113 78 219 186 smit:SMT_1078|5end_folylpolyglutamate_synthase_1016713..1016943_NEGATIVE 328 126 88 206 158 smit:SMT_705|5end_hypothetical_protein_615336..615566_NEGATIVE 323 169 122 217 172 smit:SMT_2015|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1932655..1932885_POSITIVE 320 167 122 186 130 smit:SMT_699|5end_K03106_signal_recognition_particle,_subunit_SRP54_610336..610566_NEGATIVE 315 179 132 176 134 smit:SMT_1250|5end_hypothetical_protein_1211345..1211575_NEGATIVE 313 179 134 159 116 smit:SMT_1694|5end_GTPase_1676674..1676904_POSITIVE 306 180 131 97 36 smit:SMT_2119|5end_mercuric_reductase_2005583..2005813_POSITIVE 303 179 131 108 63 smit:SMT_1791|5end_hypothetical_protein_1765907..1766137_POSITIVE 299 147 103 118 71 smit:SMT_1915|5end_pyridoxine_biosynthesis_protein_1860329..1860559_POSITIVE 296 150 102 127 68 smit:SMT_2005|5end_pullulanase,_type_I_1926289..1926519_NEGATIVE 296 150 102 221 162 smit:SMT_1967|5end_dicarboxylate/amino_acid:cation_(Na+_or_H+)_symporter_(DAACS)_family_protein_1899683..1899913_NEGATIVE 296 150 102 221 162 smit:SMT_1690|5end_K06148_ATP-binding_cassette,_subfamily_C,_bacterial_1674779..1675009_NEGATIVE 296 150 102 221 162 smit:SMT_779|5end_ribonuclease_BN,_putative_688530..688760_NEGATIVE 296 150 102 195 136 smit:SMT_304|5end_hypothetical_protein_246580..246810_POSITIVE 293 166 130 226 184 smit:SMT_1645|5end_hypothetical_protein_1631738..1631968_POSITIVE 292 168 123 54 8 smit:SMT_903|5end_hypothetical_protein_852310..852540_NEGATIVE 291 140 91 231 176 smit:SMT_1734|5end_serine/threonine_protein_phosphatase_1718558..1718788_POSITIVE 291 168 122 204 163 smit:SMT_169|5end_30S_ribosomal_protein_S10_114515..114745_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 337 184 142 80 34 smit:SMT_1620|3end_hypothetical_protein_1606695..1606845_NEGATIVE 292 168 123 70 24 smit:SMT_904|3end_hypothetical_protein_852294..852444_NEGATIVE 290 169 122 78 24 smit:SMT_2024|3end_hypothetical_protein_1940690..1940840_POSITIVE 290 169 122 67 13 smit:SMT_2002|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1924145..1924295_NEGATIVE 290 169 122 78 24 smit:SMT_1981|3end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1912868..1913018_POSITIVE 290 169 122 83 29 smit:SMT_1971|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1903526..1903676_POSITIVE 290 169 122 67 13 smit:SMT_1966|3end_DNA_polymerase_III_alpha_subunit_1898328..1898478_POSITIVE 290 169 122 78 24 smit:SMT_1942|3end_zinc_metalloprotease_ZmpB,_truncated_1878384..1878534_POSITIVE 290 169 122 67 13 smit:SMT_1927|3end_cell_division_protein_FtsH_1868408..1868558_NEGATIVE 290 169 122 78 24 smit:SMT_1926|3end_mevalonate_kinase_1868062..1868212_POSITIVE 290 169 122 78 24 smit:SMT_1887|3end_LicC_protein_1841740..1841890_POSITIVE 290 169 122 67 13 smit:SMT_1885|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1839830..1839980_NEGATIVE 290 169 122 67 13 smit:SMT_1873|3end_K02006_cobalt_transport_system_ATP-binding_protein_1832351..1832501_POSITIVE 290 169 122 83 29 smit:SMT_1852|3end_1,4-alpha-glucan_branching_enzyme_1816467..1816617_NEGATIVE 290 169 122 67 13 smit:SMT_958|3end_hypothetical_protein_predicted_by_Glimmer/Critica_905239..905389_POSITIVE 290 169 122 83 29 smit:SMT_326|3end_K02029_polar_amino_acid_transport_system_permease_protein_263217..263367_NEGATIVE 284 148 101 64 9 smit:SMT_1190|3end_phosphoribosylaminoimidazole_carboxylase,_catalytic_subunit_1147741..1147891_NEGATIVE 283 173 140 146 102 smit:SMT_987|3end_N-acetylmannosamine_kinase_/_transcriptional_regulator_931655..931805_NEGATIVE 282 178 131 41 2 smit:SMT_815|3end_hypothetical_protein_723677..723827_NEGATIVE 281 43 1 59 10 smit:SMT_148|3end_glycoprotein_endopeptidase_98601..98751_POSITIVE