Origin IGS: ttgtcacctccatatttgtaagactattataaaatccaaatatgaaatgtttatgaaatacaaaaaagagcaaaatttttttgctctacttgaattatatgaaaacaaaagagctagcctaagctaactcttatcctatgcggagagagggacttgaaccctcacgacctaaagcggtcacaggatccttagtcctgcgnnnnnttaattaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttaattaannnnncgcaggactaaggatcctgtgaccgctttaggtcgtgagggttcaagtccctctctccgcataggataagagttagcttaggctagctcttttgttttcatataattcaagtagagcaaaaaaattttgctcttttttgtatttcataaacatttcatatttggattttataatagtcttacaaatatggaggtgacaa Mask Tandem Repeat Region ================================================ ttgtcacctccatatttgtaagactattataaaatccaaatatgaaatgtttatgaaatacaaaaaagagcaaaatttttttgctctacttgaattatatgaaaacaaaagagctagcctaagctaactcttatcctatgcggagagagggacttgaaccctcacgacctaaagcggtcacaggatccttagtcctgcgnnnnnttaattaa Find is-nt database================================================ Query_seq: SMT_1522:SMT_1523|SMT_1522:SMT_1523:K02004:ferrochelatase:<-<-:1503268..1503479 212 ttgtcacctccatatttgtaagactattataaaatccaaatatgaaatgtttatgaaatacaaaaaagagcaaaatttttttgctctacttgaattatatgaaaacaaaagagctagcctaagctaactcttatcctatgcggagagagggacttgaaccctcacgacctaaagcggtcacaggatccttagtcctgcgnnnnnttaattaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1522:SMT_1523|SMT_1522:SMT_1523:K02004:ferrochelatase:<-<-:1503268..1503479 212 ttgtcacctccatatttgtaagactattataaaatccaaatatgaaatgtttatgaaatacaaaaaagagcaaaatttttttgctctacttgaattatatgaaaacaaaagagctagcctaagctaactcttatcctatgcggagagagggacttgaaccctcacgacctaaagcggtcacaggatccttagtcctgcgnnnnnttaattaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1522:SMT_1523|SMT_1522:SMT_1523:K02004:ferrochelatase:<-<-:1503268..1503479 212 ttgtcacctccatatttgtaagactattataaaatccaaatatgaaatgtttatgaaatacaaaaaagagcaaaatttttttgctctacttgaattatatgaaaacaaaagagctagcctaagctaactcttatcctatgcggagagagggacttgaaccctcacgacctaaagcggtcacaggatccttagtcctgcgnnnnnttaattaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttgtcacctccatatttgtaagactattataaaatccaaatatgaaatgtttatgaaatacaaaaaagagcaaaatttttttgctctacttgaattatatgaaaacaaaagagctagcctaagctaactcttatcctatgcggagagagggacttgaaccctcacgacctaaagcggtcacaggatccttagtcctgcgnnnnnttaattaa Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 14 End: 124 ............. M C K T I I K S K Y E M F M K Y K K E Q N F F A L L E L Y E N K R A S L S ........................................................................................ Protein_Len: 34 Strand: - Start: 7 End: 108 ...... R W I Q L V I I F D L Y S I N I F Y L F S C F K K A R S S N Y S F M ........................................................................................................ ttgtcacctccatatttgtaagactattataaaatccaaatatgaaatgtttatgaaatacaaaaaagagcaaaatttttttgctctacttgaattatatgaaaacaaaagagctagcctaagctaactcttatcctatgcggagagagggacttgaaccctcacgacctaaagcggtcacaggatccttagtcctgcgnnnnnttaattaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttgtcacctccatatttgtaagactattataaaatccaaatatgaaatgtttatgaaatacaaaaaagagcaaaatttttttgctctacttgaattatatgaaaacaaaagagctagcctaagctaactcttatcctatgcggagagagggacttgaaccctcacgacctaaagcggtcacaggatccttagtcctgcgnnnnnttaattaa Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 79 HP_score: -10.4 Tail_Score: -2.81225 Start: 68 End: 86 Full_Region: ATGAAATACAAAAAA GAGCAAAA TTT TTTTGCTC TACTTGAATTATATG ...................................................................GAGCAAAATTTTTTTGCTC.............................................................................................................................. TransTerm Strand: - Conf: 100 HP_score: -10.4 Tail_Score: -5.5762 Start: 68 End: 86 Full_Region: catataattcaagta gagcaaaa aaa ttttgctc ttttttgtatttcat ...................................................................gagcaaaaaaattttgctc.............................................................................................................................. TransTerm Strand: - Conf: 95 HP_score: -8.6 Tail_Score: -5.16132 Start: 111 End: 130 Full_Region: ctccgcataggataa gagttagc ttag gctagctc ttttgttttcatata ..............................................................................................................gagttagcttaggctagctc.................................................................................. Find igs database================================================ Query_seq: SMT_1522:SMT_1523|SMT_1522:SMT_1523:K02004:ferrochelatase:<-<-:1503268..1503479 212 ttgtcacctccatatttgtaagactattataaaatccaaatatgaaatgtttatgaaatacaaaaaagagcaaaatttttttgctctacttgaattatatgaaaacaaaagagctagcctaagctaactcttatcctatgcggagagagggacttgaaccctcacgacctaaagcggtcacaggatccttagtcctgcgnnnnnttaattaa Intra-Species Hit: Count: 1 Min: 1 Max: 199 Len: 199 Subject: smit_SMT_1522_SMT_1523|K02004:ferrochelatase|NEGATIVE:NEGATIVE|[150 3268,1503479]|212 HSP 1 e-value: 2.0E-96 bit: 353.0 Len: 199 Query Start:1 Query End:199 Subject Strand: POSITIVE Subject Start: 1 Subject End: 199 ttgtcacctccatatttgtaagactattataaaatccaaatatgaaatgtttatgaaatacaaaaaagagcaaaannnnnnngctctacttgaattatatgaaaacaaaagagctagcctaagctaactcttatcctatgcggagagagggacttgaaccctcacgacctaaagcggtcacaggatccttagtcctgcg............. ttgtcacctccatatttgtaagactattataaaatccaaatatgaaatgtttatgaaatacaaaaaagagcaaaatttttttgctctacttgaattatatgaaaacaaaagagctagcctaagctaactcttatcctatgcggagagagggacttgaaccctcacgacctaaagcggtcacaggatccttagtcctgcg............. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGUCACCUCCAUAUUUGUAAGACUAUUAUAAAAUCCAAAUAUGAAAUGUUUAUGAAAUACAAAAAAGAGCAAAAUUUUUUUGCUCUACUUGAAUUAUAUGAAAACAAAAGAGCUAGCCUAAGCUAACUCUUAUCCUAUGCGGAGAGAGGGACUUGAACCCUCACGACCUAAAGCGGUCACAGGAUCCUUAGUCCUGCG .......((((...(((((((.....)))))))......(((.((....((((((.(((.(((...((((((((.....))))))))..))).))).)))))).....(((((.((((....)))).))))).)).)))..)))).(((((.......)))))..((((......)))).((((((.....)))))).. (-51.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGCAGGACUAAGGAUCCUGUGACCGCUUUAGGUCGUGAGGGUUCAAGUCCCUCUCUCCGCAUAGGAUAAGAGUUAGCUUAGGCUAGCUCUUUUGUUUUCAUAUAAUUCAAGUAGAGCAAAAAAAUUUUGCUCUUUUUUGUAUUUCAUAAACAUUUCAUAUUUGGAUUUUAUAAUAGUCUUACAAAUAUGGAGGUGACAA .(((((((....).))))))((((......))))..(((((.......)))))..(((.....))).((((((((((....)))))))))).(((((......(((.((((.((((((((.....))))))))..)))).)))....))))).((((((((((((((.......))))...))))))))))(....).. (-59.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 815 130 81 221 172 smit:SMT_1522|5end_K02004_1503177..1503407_NEGATIVE 378 177 140 230 187 smit:SMT_2052|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1961591..1961821_NEGATIVE 322 188 141 119 54 smit:SMT_1800|5end_exonuclease_RexA_1773788..1774018_NEGATIVE 319 180 139 124 60 smit:SMT_2023|5end_tryptophan_synthase,_alpha_subunit_1939315..1939545_POSITIVE 319 180 139 124 60 smit:SMT_1818|5end_tryptophan_synthase,_alpha_subunit_1792190..1792420_POSITIVE 311 170 125 230 186 smit:SMT_1132|5end_glutamate--cysteine_ligase,_putative,_truncated_1068108..1068338_NEGATIVE 304 169 121 209 142 smit:SMT_1088|5end_hypothetical_protein_1024346..1024576_NEGATIVE 303 177 133 95 38 smit:SMT_525|5end_30S_ribosomal_protein_S16_450875..451105_POSITIVE 303 177 133 77 20 smit:SMT_509|5end_30S_ribosomal_protein_S16_438746..438976_POSITIVE 301 166 121 163 113 smit:SMT_407|5end_para-aminobenzoate_synthetase_component_I_/_4-amino-4-deoxychorismate_lyase_351365..351595_POSITIVE 300 189 142 130 73 smit:SMT_403|5end_two-component_sensor_kinase_YesM_331580..331810_POSITIVE 299 179 133 230 185 smit:SMT_1643|5end_2-isopropylmalate_synthase_1629130..1629360_POSITIVE 298 190 146 139 84 smit:SMT_2162|5end_hypothetical_protein_2036931..2037161_POSITIVE 294 173 139 77 40 smit:SMT_416|5end_ribonuclease_Z_359423..359653_POSITIVE 293 160 111 60 12 smit:SMT_2083|5end_K06147_ATP-binding_cassette,_subfamily_B,_bacterial_1982411..1982641_POSITIVE 292 170 122 220 173 smit:SMT_755|5end_shikimate_5-dehydrogenase_659917..660147_NEGATIVE 287 159 111 204 158 smit:SMT_1662|5end_histidine_protein_kinase_1652357..1652587_NEGATIVE 284 159 112 214 158 smit:SMT_1630|5end_hypothetical_protein_1617291..1617521_POSITIVE 284 159 112 214 158 smit:SMT_1241|5end_hypothetical_protein_1201040..1201270_POSITIVE 284 186 142 184 133 smit:SMT_189|5end_50S_ribosomal_protein_L15_123832..124062_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 313 179 135 52 10 smit:SMT_140|3end_NUDIX_family_hydrolase_91498..91648_POSITIVE 300 189 142 133 76 smit:SMT_402|3end_two-component_response_regulator_YesN_331663..331813_POSITIVE 294 180 132 145 100 smit:SMT_2046|3end_choline_binding_protein_C_1957280..1957430_NEGATIVE 294 173 139 62 25 smit:SMT_415|3end_hypothetical_protein_359488..359638_POSITIVE 288 160 111 77 16 smit:SMT_1785|3end_phenylalanyl-tRNA_synthetase_beta_chain_1762327..1762477_NEGATIVE 282 188 141 52 7 smit:SMT_1695|3end_ribulose-phosphate_3-epimerase_1678299..1678449_POSITIVE 279 179 142 64 6 smit:SMT_1214|3end_K03584_DNA_repair_protein_RecO_(recombination_protein_O)_1172652..1172802_NEGATIVE 272 142 107 118 72 smit:SMT_154|3end_K03647_protein_involved_in_ribonucleotide_reduction_102474..102624_POSITIVE 271 148 107 145 107 smit:SMT_330|3end_membrane-associated_phospholipid_phosphatase_266187..266337_POSITIVE 270 180 131 62 12 smit:SMT_853|3end_ATP-dependent_endopeptidase_clp_ATP-binding_subunit_ClpX_798053..798203_NEGATIVE 269 189 143 76 26 smit:SMT_1933|3end_K02006_cobalt_transport_system_ATP-binding_protein_1871986..1872136_NEGATIVE 267 176 134 68 12 smit:SMT_860|3end_homoserine_O-succinyltransferase_804067..804217_NEGATIVE 266 158 114 59 17 smit:SMT_1805|3end_2,3,4,5-tetrahydropyridine-2-carboxylate_N-succinyltransferase,_putative_1781241..1781391_POSITIVE 266 158 114 59 17 smit:SMT_1634|3end_2,3,4,5-tetrahydropyridine-2-carboxylate_N-succinyltransferase,_putative_1621438..1621588_POSITIVE 266 147 110 40 1 smit:SMT_671|3end_hypothetical_protein_580676..580826_POSITIVE 265 168 134 150 116 smit:SMT_2026|3end_K03466_DNA_segregation_ATPase_FtsK/SpoIIIE,_S-DNA-T_family_1941905..1942055_NEGATIVE 262 151 118 146 113 smit:SMT_417|3end_short_chain_dehydrogenase_361208..361358_POSITIVE 252 169 124 150 100 smit:SMT_234|3end_aminopeptidase_PepS_174151..174301_POSITIVE 250 156 111 73 20 smit:SMT_1941|3end_hypothetical_protein_1877663..1877813_POSITIVE 248 187 146 49 12 smit:SMT_1212|3end_acyl_carrier_protein,_putative_1171424..1171574_NEGATIVE