Origin IGS: aagaaataaaaaaatgcaagacatgtactagggatttaccatcaagttataagcacaataagtgcgaatcgtgtagaaacaaaaaaatagatgaagcaaaaaagatacttaaagttgttggacaagttggcagtgtggttactccagttgtagttggtatgttgatgaataaaactaagaaaaagaagaaataaactaatctattaagtacctagaaaggaaaactctcaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttgagagttttcctttctaggtacttaatagattagtttatttcttctttttcttagttttattcatcaacataccaactacaactggagtaaccacactgccaacttgtccaacaactttaagtatcttttttgcttcatctatttttttgtttctacacgattcgcacttattgtgcttataacttgatggtaaatccctagtacatgtcttgcatttttttatttctt Mask Tandem Repeat Region ================================================ aagaaataaaaaaatgcaagacatgtactagggatttaccatcaagttataagcacaataagtgcgaatcgtgtagaaacaaaaaaatagatgaagcaaaaaagatacttaaagttgttggacaagttggcagtgtggttactccagttgtagttggtatgttgatgaataaaactaagaaaaagaagaaataaactaatctattaagtacctagaaaggaaaactctcaaa Find is-nt database================================================ Query_seq: SMT_1394:SMT_1395|SMT_1394:SMT_1395:hypothetical protein predicted by Glimmer/Critica:K07025 putative hydrolase of the HAD superfamily:->->:1371218..1371449 232 aagaaataaaaaaatgcaagacatgtactagggatttaccatcaagttataagcacaataagtgcgaatcgtgtagaaacaaaaaaatagatgaagcaaaaaagatacttaaagttgttggacaagttggcagtgtggttactccagttgtagttggtatgttgatgaataaaactaagaaaaagaagaaataaactaatctattaagtacctagaaaggaaaactctcaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1394:SMT_1395|SMT_1394:SMT_1395:hypothetical protein predicted by Glimmer/Critica:K07025 putative hydrolase of the HAD superfamily:->->:1371218..1371449 232 aagaaataaaaaaatgcaagacatgtactagggatttaccatcaagttataagcacaataagtgcgaatcgtgtagaaacaaaaaaatagatgaagcaaaaaagatacttaaagttgttggacaagttggcagtgtggttactccagttgtagttggtatgttgatgaataaaactaagaaaaagaagaaataaactaatctattaagtacctagaaaggaaaactctcaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1394:SMT_1395|SMT_1394:SMT_1395:hypothetical protein predicted by Glimmer/Critica:K07025 putative hydrolase of the HAD superfamily:->->:1371218..1371449 232 aagaaataaaaaaatgcaagacatgtactagggatttaccatcaagttataagcacaataagtgcgaatcgtgtagaaacaaaaaaatagatgaagcaaaaaagatacttaaagttgttggacaagttggcagtgtggttactccagttgtagttggtatgttgatgaataaaactaagaaaaagaagaaataaactaatctattaagtacctagaaaggaaaactctcaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aagaaataaaaaaatgcaagacatgtactagggatttaccatcaagttataagcacaataagtgcgaatcgtgtagaaacaaaaaaatagatgaagcaaaaaagatacttaaagttgttggacaagttggcagtgtggttactccagttgtagttggtatgttgatgaataaaactaagaaaaagaagaaataaactaatctattaagtacctagaaaggaaaactctcaaa Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 6 End: 191 ..... M K K C K T C T R D L P S S Y K H N K C E S C R N K K I D E A K K I L K V V G Q V G S V V T P V V V G M L M N K T K K K ......................................... Protein_Len: 39 Strand: - Start: 50 End: 166 ................................................. L C L L H S D H L F L F I S S A F F I S L T T P C T P L T T V G T T T P I N M .................................................................. Protein_Len: 52 Strand: - Start: 45 End: 200 ............................................ T I L V I L A F R T S V F F Y I F C F L Y K F N N S L N A T H N S W N Y N T H Q H I F S L F L L F L S M ................................ aagaaataaaaaaatgcaagacatgtactagggatttaccatcaagttataagcacaataagtgcgaatcgtgtagaaacaaaaaaatagatgaagcaaaaaagatacttaaagttgttggacaagttggcagtgtggttactccagttgtagttggtatgttgatgaataaaactaagaaaaagaagaaataaactaatctattaagtacctagaaaggaaaactctcaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aagaaataaaaaaatgcaagacatgtactagggatttaccatcaagttataagcacaataagtgcgaatcgtgtagaaacaaaaaaatagatgaagcaaaaaagatacttaaagttgttggacaagttggcagtgtggttactccagttgtagttggtatgttgatgaataaaactaagaaaaagaagaaataaactaatctattaagtacctagaaaggaaaactctcaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1394:SMT_1395|SMT_1394:SMT_1395:hypothetical protein predicted by Glimmer/Critica:K07025 putative hydrolase of the HAD superfamily:->->:1371218..1371449 232 aagaaataaaaaaatgcaagacatgtactagggatttaccatcaagttataagcacaataagtgcgaatcgtgtagaaacaaaaaaatagatgaagcaaaaaagatacttaaagttgttggacaagttggcagtgtggttactccagttgtagttggtatgttgatgaataaaactaagaaaaagaagaaataaactaatctattaagtacctagaaaggaaaactctcaaa Intra-Species Hit: Count: 1 Min: 15 Max: 232 Len: 218 Subject: smit_SMT_1394_SMT_1395|hypothetical protein predicted by Glimmer/Critica:K07025 putative hydrolase of the HAD superfamily|POSITIVE:POSITIVE|[1371218,1371449]|232 HSP 1 e-value: 1.0E-108 bit: 391.0 Len: 218 Query Start:15 Query End:232 Subject Strand: POSITIVE Subject Start: 15 Subject End: 232 ..............tgcaagacatgtactagggatttaccatcaagttataagcacaataagtgcgaatcgtgtagaaacnnnnnnntagatgaagcaaaaaagatacttaaagttgttggacaagttggcagtgtggttactccagttgtagttggtatgttgatgaataaaactaagaaaaagaagaaataaactaatctattaagtacctagaaaggaaaactctcaaa ..............tgcaagacatgtactagggatttaccatcaagttataagcacaataagtgcgaatcgtgtagaaacaaaaaaatagatgaagcaaaaaagatacttaaagttgttggacaagttggcagtgtggttactccagttgtagttggtatgttgatgaataaaactaagaaaaagaagaaataaactaatctattaagtacctagaaaggaaaactctcaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGCAAGACAUGUACUAGGGAUUUACCAUCAAGUUAUAAGCACAAUAAGUGCGAAUCGUGUAGAAACAAAAAAAUAGAUGAAGCAAAAAAGAUACUUAAAGUUGUUGGACAAGUUGGCAGUGUGGUUACUCCAGUUGUAGUUGGUAUGUUGAUGAAUAAAACUAAGAAAAAGAAGAAAUAAACUAAUCUAUUAAGUACCUAGAAAGGAAAACUCUCAAA .............(((((.......((((.........((((.....)))).....((......)).........))))............(((((((.........((((.((((.((((....)))))))))))).((((((.((((..........................)))).))))))...))))))))))))................. (-33.07) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUGAGAGUUUUCCUUUCUAGGUACUUAAUAGAUUAGUUUAUUUCUUCUUUUUCUUAGUUUUAUUCAUCAACAUACCAACUACAACUGGAGUAACCACACUGCCAACUUGUCCAACAACUUUAAGUAUCUUUUUUGCUUCAUCUAUUUUUUUGUUUCUACACGAUUCGCACUUAUUGUGCUUAUAACUUGAUGGUAAAUCCCUAGUACAUGUCUUGCA ...(((((.....))))).((((((((((.((.......................(((((.(((........)))..)))))....((((((......))).))).............))))))))))))....(((..(((.((((.....(((((((((..((..((((.....))))..))....)).))).))))....)))).)))....))) (-26.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 331 140 94 79 35 smit:SMT_753|5end_chorismate_synthase_657967..658197_NEGATIVE 299 67 24 72 20 smit:SMT_1293|5end_K02051_sulfonate/nitrate/taurine_transport_system_substrate-binding_protein_1254976..1255206_POSITIVE 297 144 102 216 162 smit:SMT_1010|5end_hypothetical_protein_948292..948522_POSITIVE 297 144 102 130 76 smit:SMT_1009|5end_hypothetical_protein_predicted_by_Glimmer/Critica_948206..948436_POSITIVE 296 144 101 62 27 smit:SMT_413|5end_tRNA_delta(2)-isopentenylpyrophosphate_transferase_356677..356907_POSITIVE 288 153 112 190 151 smit:SMT_2014|5end_tryptophan_synthase_beta_chain_1932471..1932701_POSITIVE 284 118 71 66 10 smit:SMT_1498|5end_transcriptional_regulator,_LytR_family_1481958..1482188_POSITIVE 273 150 103 217 167 smit:SMT_389|5end_PTS_system,_galactitol-specific_IIA_component,_putative_321779..322009_POSITIVE 272 152 112 97 50 smit:SMT_1558|5end_hypothetical_protein_1544058..1544288_NEGATIVE 271 150 105 147 92 smit:SMT_489|5end_K01999_branched-chain_amino_acid_transport_system_substrate-binding_protein_423669..423899_POSITIVE 264 150 101 76 12 smit:SMT_904|5end_hypothetical_protein_852698..852928_NEGATIVE 259 150 106 63 11 smit:SMT_1460|5end_K02038_phosphate_transport_system_permease_protein_1441429..1441659_POSITIVE 250 149 107 205 145 smit:SMT_131|5end_phosphatase,_putative_83663..83893_NEGATIVE 248 149 104 85 35 smit:SMT_811|5end_ATP-dependent_RNA_helicase_721025..721255_POSITIVE 247 149 111 65 20 smit:SMT_2127|5end_exodeoxyribonuclease_V_alpha_chain_2009306..2009536_POSITIVE 246 147 103 189 129 smit:SMT_1256|5end_ketol-acid_reductoisomerase_1222631..1222861_NEGATIVE 246 137 93 98 49 smit:SMT_438|5end_hypothetical_protein_predicted_by_Glimmer/Critica_379136..379366_POSITIVE 245 148 101 50 6 smit:SMT_272|5end_hypothetical_protein_213315..213545_NEGATIVE 244 153 111 66 29 smit:SMT_2171|5end_UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-_diaminopimelate--D-alanyl-D-alanine_ligase_2042271..2042501_POSITIVE 244 143 103 190 147 smit:SMT_543|5end_topoisomerase_IV_subunit_B_458372..458602_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 331 140 94 69 25 smit:SMT_754|3end_3-dehydroquinate_synthase_657977..658127_NEGATIVE 289 120 74 138 92 smit:SMT_326|3end_K02029_polar_amino_acid_transport_system_permease_protein_263217..263367_NEGATIVE 284 118 71 58 2 smit:SMT_1497|3end_acetyltransferase,_GNAT_family_1482030..1482180_POSITIVE 277 160 111 59 7 smit:SMT_1041|3end_K07586_hypothetical_protein_984164..984314_POSITIVE 274 143 101 134 76 smit:SMT_2115|3end_zinc_metalloprotease_ZmpB,_putative_2003202..2003352_POSITIVE 273 150 103 145 95 smit:SMT_1985|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1914407..1914557_NEGATIVE 268 149 112 45 2 smit:SMT_2086|3end_hypothetical_protein_1983331..1983481_NEGATIVE 259 150 106 73 21 smit:SMT_1459|3end_K02037_phosphate_transport_system_permease_protein_1441519..1441669_POSITIVE 254 109 71 138 100 smit:SMT_603|3end_hypothetical_protein_518532..518682_POSITIVE 249 150 103 148 108 smit:SMT_1956|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1889537..1889687_NEGATIVE 248 149 104 88 38 smit:SMT_810|3end_oxidoreductase_721108..721258_POSITIVE 246 149 103 92 43 smit:SMT_1955|3end_carbamoyl-phosphate_synthase_small_chain_1888992..1889142_NEGATIVE 246 147 103 123 63 smit:SMT_1257|3end_acetolactate_synthase_small_subunit_1222697..1222847_NEGATIVE 245 140 99 149 115 smit:SMT_436|3end_amidase_related_to_nicotinamidase_377575..377725_NEGATIVE 244 143 103 128 85 smit:SMT_2044|3end_transcriptional_activator_TipA,_putative_1955274..1955424_NEGATIVE 244 143 103 128 85 smit:SMT_2026|3end_K03466_DNA_segregation_ATPase_FtsK/SpoIIIE,_S-DNA-T_family_1941905..1942055_NEGATIVE 244 143 103 128 85 smit:SMT_2016|3end_tyrosine_recombinase_1933021..1933171_NEGATIVE 244 143 103 128 85 smit:SMT_2006|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1926803..1926953_POSITIVE 244 143 103 138 95 smit:SMT_2001|3end_membrane_protease_protein_family_1923889..1924039_POSITIVE 244 143 103 128 85 smit:SMT_1957|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1890829..1890979_POSITIVE