Origin IGS: gacactaatacctctttgggagtgtgaagacaagtttcacattgaaaaagccaaccgaacttgcctagctttcgaatttcttggctcaggctgaaaaagtccacaggacttacttcgctttcaagtttcttggctcaggctgaaaaagtccacaggactttttcactcccacaaatctttctaattttttcttctactagtataacaaaaaaagggaagaaggcaaacttccctgt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 acagggaagtttgccttcttccctttttttgttatactagtagaagaaaaaattagaaagatttgtgggagtgaaaaagtcctgtggactttttcagcctgagccaagaaacttgaaagcgaagtaagtcctgtggactttttcagcctgagccaagaaattcgaaagctaggcaagttcggttggctttttcaatgtgaaacttgtcttcacactcccaaagaggtattagtgtc Mask Tandem Repeat Region ================================================ gacactaatacctctttgggagtgtgaagacaagtttcacattgaaaaagccaaccgaacttgcctagctttcgaatttcttggctcaggctgaaaaagtccacaggacttacttcgctttcaagtttcttggctcaggctgaaaaagtccacaggactttttcactcccacaaatctttctaattttttcttctactagtataacaaaaaaagggaagaaggcaaacttccctgt Find is-nt database================================================ Query_seq: SMT_1375:SMT_1376|SMT_1375:SMT_1376:putative glucose-inhibited division protein:tetrapyrrole (corrin/porphyrin) methylase family protein:<-<-:1354529..1354764 236 gacactaatacctctttgggagtgtgaagacaagtttcacattgaaaaagccaaccgaacttgcctagctttcgaatttcttggctcaggctgaaaaagtccacaggacttacttcgctttcaagtttcttggctcaggctgaaaaagtccacaggactttttcactcccacaaatctttctaattttttcttctactagtataacaaaaaaagggaagaaggcaaacttccctgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1375:SMT_1376|SMT_1375:SMT_1376:putative glucose-inhibited division protein:tetrapyrrole (corrin/porphyrin) methylase family protein:<-<-:1354529..1354764 236 gacactaatacctctttgggagtgtgaagacaagtttcacattgaaaaagccaaccgaacttgcctagctttcgaatttcttggctcaggctgaaaaagtccacaggacttacttcgctttcaagtttcttggctcaggctgaaaaagtccacaggactttttcactcccacaaatctttctaattttttcttctactagtataacaaaaaaagggaagaaggcaaacttccctgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1375:SMT_1376|SMT_1375:SMT_1376:putative glucose-inhibited division protein:tetrapyrrole (corrin/porphyrin) methylase family protein:<-<-:1354529..1354764 236 gacactaatacctctttgggagtgtgaagacaagtttcacattgaaaaagccaaccgaacttgcctagctttcgaatttcttggctcaggctgaaaaagtccacaggacttacttcgctttcaagtttcttggctcaggctgaaaaagtccacaggactttttcactcccacaaatctttctaattttttcttctactagtataacaaaaaaagggaagaaggcaaacttccctgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gacactaatacctctttgggagtgtgaagacaagtttcacattgaaaaagccaaccgaacttgcctagctttcgaatttcttggctcaggctgaaaaagtccacaggacttacttcgctttcaagtttcttggctcaggctgaaaaagtccacaggactttttcactcccacaaatctttctaattttttcttctactagtataacaaaaaaagggaagaaggcaaacttccctgt Predict ORF larger than 30AA ================================================ Protein_Len: 58 Strand: + Start: 61 End: 234 ............................................................ M P S F R I S W L R L K K S T G L T S L S S F L A Q A E K V H R T F S L P Q I F L I F S S T S I T K K G K K A N F P .. Protein_Len: 34 Strand: - Start: 3 End: 104 .. V L V E K P T H L C T E C Q F L W G F K G L K R I E Q S L S F F D M .................................................................................................................................... gacactaatacctctttgggagtgtgaagacaagtttcacattgaaaaagccaaccgaacttgcctagctttcgaatttcttggctcaggctgaaaaagtccacaggacttacttcgctttcaagtttcttggctcaggctgaaaaagtccacaggactttttcactcccacaaatctttctaattttttcttctactagtataacaaaaaaagggaagaaggcaaacttccctgt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gacactaatacctctttgggagtgtgaagacaagtttcacattgaaaaagccaaccgaacttgcctagctttcgaatttcttggctcaggctgaaaaagtccacaggacttacttcgctttcaagtttcttggctcaggctgaaaaagtccacaggactttttcactcccacaaatctttctaattttttcttctactagtataacaaaaaaagggaagaaggcaaacttccctgt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 79 HP_score: -6.8 Tail_Score: -4.59773 Start: 99 End: 109 Full_Region: ttgaaagcgaagtaa gtcc tgt ggac tttttcagcctgagc ..................................................................................................gtcctgtggac............................................................................................................................... TransTerm Strand: + Conf: 79 HP_score: -6.8 Tail_Score: -4.65895 Start: 148 End: 158 Full_Region: GCTCAGGCTGAAAAA GTCC ACA GGAC TTTTTCACTCCCACA ...................................................................................................................................................GTCCACAGGAC.............................................................................. TransTerm Strand: - Conf: 79 HP_score: -6.8 Tail_Score: -4.59773 Start: 148 End: 158 Full_Region: tgtgggagtgaaaaa gtcc tgt ggac tttttcagcctgagc ...................................................................................................................................................gtcctgtggac.............................................................................. Find igs database================================================ Query_seq: SMT_1375:SMT_1376|SMT_1375:SMT_1376:putative glucose-inhibited division protein:tetrapyrrole (corrin/porphyrin) methylase family protein:<-<-:1354529..1354764 236 gacactaatacctctttgggagtgtgaagacaagtttcacattgaaaaagccaaccgaacttgcctagctttcgaatttcttggctcaggctgaaaaagtccacaggacttacttcgctttcaagtttcttggctcaggctgaaaaagtccacaggactttttcactcccacaaatctttctaattttttcttctactagtataacaaaaaaagggaagaaggcaaacttccctgt Intra-Species Hit: Count: 1 Min: 1 Max: 236 Len: 236 Subject: smit_SMT_1375_SMT_1376|putative glucose-inhibited division protein:tetrapyrrole (corrin/porphyrin) methylase family protein|NEGATIVE:NEGATIVE|[1354529,1354764]|236 HSP 1 e-value: 1.0E-118 bit: 426.0 Len: 236 Query Start:1 Query End:236 Subject Strand: POSITIVE Subject Start: 1 Subject End: 236 gacactaatacctctttgggagtgtgaagacaagtttcacattgaaaaagccaaccgaacttgcctagctttcgaatttcttggctcaggctgaaaaagtccacaggacttacttcgctttcaagtttcttggctcaggctgaaaaagtccacaggactttttcactcccacaaatctttctaattttttcttctactagtataacnnnnnnngggaagaaggcaaacttccctgt gacactaatacctctttgggagtgtgaagacaagtttcacattgaaaaagccaaccgaacttgcctagctttcgaatttcttggctcaggctgaaaaagtccacaggacttacttcgctttcaagtttcttggctcaggctgaaaaagtccacaggactttttcactcccacaaatctttctaattttttcttctactagtataacaaaaaaagggaagaaggcaaacttccctgt Inter-species Hit: Count: 1 Min: 1 Max: 236 Len: 236 Subject: NC_003098_spr0843_spr0844|hypothetical protein:glucose-inhibited division protein A|POSITIVE:POSITIVE|[841561,841804]|244 HSP 1 e-value: 3.0E-42 bit: 172.0 Len: 120 Query Start:117 Query End:236 Subject Strand: NEGATIVE Subject Start: 60 Subject End: 178 ....................................................................................................................gctttcaagtttcttggctcaggctgaaaaagtccacaggactttttcactcccacaaatctttctaattttttcttctactagtataacnnnnnnngggaagaaggcaaacttccctgt ....................................................................................................................gctttcgagtttcttggctcaggctgaaaaagtccacaggactttttcactcccacaaatctttct-attttttcttctactagtataacaaaaaaagggaagaaggcaaacttccctgt HSP 2 e-value: 7.0E-28 bit: 125.0 Len: 111 Query Start:1 Query End:111 Subject Strand: NEGATIVE Subject Start: 135 Subject End: 244 gacactaatacctctttgggagtgtgaagacaagtttcacattgaaaaagccaaccgaacttgcctagctttcgaatttcttggctcaggctgaaaaagtccacaggactt............................................................................................................................. gacactaatacctctttgggagtatgaagttaagattcacattgaaaaagccaatcagacttacg-agctttcgagtttcttggctcaggctgaaaaagtccacaggactt............................................................................................................................. Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GACACUAAUACCUCUUUGGGAGUGUGAAGACAAGUUUCACAUUGAAAAAGCCAACCGAACUUGCCUAGCUUUCGAAUUUCUUGGCUCAGGCUGAAAAAGUCCACAGGACUUACUUCGCUUUCAAGUUUCUUGGCUCAGGCUGAAAAAGUCCACAGGACUUUUUCACUCCCACAAAUCUUUCUAAUUUUUUCUUCUACUAGUAUAACAAAAAAAGGGAAGAAGGCAAACUUCCCUGU ................((((((.((((((.....))))))........((((((..(((((((...(((...........(..((....))..)..((((((...)))))).....)))..))))))).))))))......((((((((((...)))))))))).)))))).........................................(((((((........))))))).. (-62.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACAGGGAAGUUUGCCUUCUUCCCUUUUUUUGUUAUACUAGUAGAAGAAAAAAUUAGAAAGAUUUGUGGGAGUGAAAAAGUCCUGUGGACUUUUUCAGCCUGAGCCAAGAAACUUGAAAGCGAAGUAAGUCCUGUGGACUUUUUCAGCCUGAGCCAAGAAAUUCGAAAGCUAGGCAAGUUCGGUUGGCUUUUUCAAUGUGAAACUUGUCUUCACACUCCCAAAGAGGUAUUAGUGUC ..(((((((........))))))).........(((((((((.......(((((.....))))).(((((((((((((((((...)))))))))))....(((((((..((((.......((((.((((((...)))))))))).((((.(((...(.....)....))))))).))))...)))))))......((((((.......))))))))))))......))))))))). (-68.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 948 110 61 201 152 smit:SMT_1375|5end_putative_glucose-inhibited_division_protein_1354438..1354668_NEGATIVE 345 174 132 66 23 smit:SMT_847|5end_thioredoxin_reductase_792800..793030_POSITIVE 323 110 61 127 82 smit:SMT_1681|5end_D-alanine-activating_enzyme_1664184..1664414_POSITIVE 321 109 67 109 68 smit:SMT_1609|5end_hypothetical_protein_1598113..1598343_POSITIVE 296 143 102 165 129 smit:SMT_1425|5end_hypothetical_protein_1410805..1411035_NEGATIVE 294 169 124 217 175 smit:SMT_815|5end_hypothetical_protein_724294..724524_NEGATIVE 293 170 127 139 96 smit:SMT_1738|5end_peptidyl-prolyl_cis-trans_isomerase_1720497..1720727_POSITIVE 293 120 73 53 5 smit:SMT_1490|5end_putative_undecaprenyl-phosphate_galactose_phosphotransferase_1470853..1471083_POSITIVE 293 130 84 96 45 smit:SMT_361|5end_hypothetical_protein_295914..296144_POSITIVE 291 143 101 177 138 smit:SMT_455|5end_cation_transport_ATPase_391670..391900_POSITIVE 290 93 51 51 6 smit:SMT_1131|5end_threonine_synthase_1066755..1066985_NEGATIVE 288 109 63 51 11 smit:SMT_302|5end_K07444_putative_N6-adenine-specific_DNA_methylase_244808..245038_POSITIVE 287 110 62 201 140 smit:SMT_949|5end_glycosyl_transferase,_family_2_897693..897923_NEGATIVE 287 93 55 193 155 smit:SMT_310|5end_3-oxoacyl-_251370..251600_POSITIVE 286 120 74 204 157 smit:SMT_398|5end_hypothetical_protein_327651..327881_POSITIVE 284 101 61 181 146 smit:SMT_2132|5end_UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-_diaminopimelate--D-alanyl-D-alanine_ligase_2012663..2012893_POSITIVE 284 120 72 186 133 smit:SMT_1426|5end_K03095_SprT-like_protein_1411313..1411543_NEGATIVE 280 85 60 212 189 smit:SMT_1568|5end_putative_transketolase_C-terminal_section_1560122..1560352_POSITIVE 275 93 55 203 162 smit:SMT_624|5end_K07584_hypothetical_protein_529870..530100_POSITIVE 271 140 103 116 69 smit:SMT_1852|5end_1,4-alpha-glucan_branching_enzyme_1817690..1817920_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 493 120 76 47 2 smit:SMT_397|3end_NAD(+)_synthetase_327423..327573_POSITIVE 395 109 62 86 39 smit:SMT_159|3end_hypothetical_protein_104615..104765_POSITIVE 321 109 67 112 71 smit:SMT_1608|3end_hypothetical_protein_1598196..1598346_POSITIVE 296 143 102 106 70 smit:SMT_1426|3end_K03095_SprT-like_protein_1410864..1411014_NEGATIVE 295 160 113 88 42 smit:SMT_625|3end_50S_ribosomal_protein_L27_530400..530550_POSITIVE 293 130 84 103 52 smit:SMT_360|3end_K07742_hypothetical_protein_296001..296151_POSITIVE 287 93 55 149 111 smit:SMT_309|3end_acyl-carrier-protein_S-malonyltransferase_251406..251556_POSITIVE 283 119 80 47 13 smit:SMT_1580|3end_MutT/NUDIX_family_protein_1573153..1573303_POSITIVE 275 93 55 65 24 smit:SMT_623|3end_50S_ribosomal_protein_L21_529812..529962_POSITIVE 271 140 103 119 72 smit:SMT_1853|3end_1,4-alpha-glucan_branching_enzyme_1817687..1817837_NEGATIVE 270 158 111 55 8 smit:SMT_1423|3end_hypothetical_protein_1409811..1409961_POSITIVE 270 217 176 88 43 smit:SMT_161|3end_putative_folyl-polyglutamate_synthetase_106586..106736_POSITIVE 266 108 61 60 4 smit:SMT_1735|3end_K03596_GTP-binding_protein_LepA_1719549..1719699_POSITIVE 266 170 129 35 6 smit:SMT_1669|3end_potassium_uptake_protein_KtrB_1658302..1658452_NEGATIVE 263 127 81 110 63 smit:SMT_395|3end_hypothetical_protein_325027..325177_POSITIVE 262 95 51 49 3 smit:SMT_1738|3end_peptidyl-prolyl_cis-trans_isomerase_1721341..1721491_POSITIVE 260 139 93 103 59 smit:SMT_1186|3end_hypothetical_protein_1144886..1145036_POSITIVE 259 160 120 37 1 smit:SMT_1680|3end_hypothetical_protein_1664173..1664323_POSITIVE 258 66 23 68 29 smit:SMT_1017|3end_transcriptional_regulator,_MerR_family_954095..954245_NEGATIVE 257 142 101 86 48 smit:SMT_1845|3end_K01191_alpha-mannosidase_1810435..1810585_POSITIVE