Origin IGS: agtatcaccaatctaatgagaacaaaagaaattattcccaaattccgtttattttctagttttgattgctattataccattttttccgaaagaaaaaaactcttacccttaagatagaagattttttcataagaaaaagagcacttggctcttttctgtt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aacagaaaagagccaagtgctctttttcttatgaaaaaatcttctatcttaagggtaagagtttttttctttcggaaaaaatggtataatagcaatcaaaactagaaaataaacggaatttgggaataatttcttttgttctcattagattggtgatact Mask Tandem Repeat Region ================================================ agtatcaccaatctaatgagaacaaaagaaattattcccaaattccgtttattttctagttttgattgctattataccattttttccgaaagaaaaaaactcttacccttaagatagaagattttttcataagaaaaagagcacttggctcttttctgtt Find is-nt database================================================ Query_seq: SMT_1341:SMT_1342|SMT_1341:SMT_1342:putative cell division protein:protease maturation protein, putative:<-<-:1319206..1319365 160 agtatcaccaatctaatgagaacaaaagaaattattcccaaattccgtttattttctagttttgattgctattataccattttttccgaaagaaaaaaactcttacccttaagatagaagattttttcataagaaaaagagcacttggctcttttctgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1341:SMT_1342|SMT_1341:SMT_1342:putative cell division protein:protease maturation protein, putative:<-<-:1319206..1319365 160 agtatcaccaatctaatgagaacaaaagaaattattcccaaattccgtttattttctagttttgattgctattataccattttttccgaaagaaaaaaactcttacccttaagatagaagattttttcataagaaaaagagcacttggctcttttctgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1341:SMT_1342|SMT_1341:SMT_1342:putative cell division protein:protease maturation protein, putative:<-<-:1319206..1319365 160 agtatcaccaatctaatgagaacaaaagaaattattcccaaattccgtttattttctagttttgattgctattataccattttttccgaaagaaaaaaactcttacccttaagatagaagattttttcataagaaaaagagcacttggctcttttctgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agtatcaccaatctaatgagaacaaaagaaattattcccaaattccgtttattttctagttttgattgctattataccattttttccgaaagaaaaaaactcttacccttaagatagaagattttttcataagaaaaagagcacttggctcttttctgtt Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 4 End: 114 ... M T N L M R T K E I I P K F R L F S S F D C Y Y T I F S E R K K L L P L R .............................................. Protein_Len: 31 Strand: + Start: 66 End: 158 ................................................................. M L L Y H F F R K K K T L T L K I E D F F I R K R A L G S F L .. agtatcaccaatctaatgagaacaaaagaaattattcccaaattccgtttattttctagttttgattgctattataccattttttccgaaagaaaaaaactcttacccttaagatagaagattttttcataagaaaaagagcacttggctcttttctgtt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 71 End: 100 ......................................................................GTTTTTTTCTTTCGGAAAAAATGGTATAAT............................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agtatcaccaatctaatgagaacaaaagaaattattcccaaattccgtttattttctagttttgattgctattataccattttttccgaaagaaaaaaactcttacccttaagatagaagattttttcataagaaaaagagcacttggctcttttctgtt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 43 HP_score: -2.5 Tail_Score: -3.21695 Start: 17 End: 40 Full_Region: aaaataaacggaatt tgggaataa tttctt ttgttctca ttagattggtgatac ................tgggaataatttcttttgttctca........................................................................................................................ Find igs database================================================ Query_seq: SMT_1341:SMT_1342|SMT_1341:SMT_1342:putative cell division protein:protease maturation protein, putative:<-<-:1319206..1319365 160 agtatcaccaatctaatgagaacaaaagaaattattcccaaattccgtttattttctagttttgattgctattataccattttttccgaaagaaaaaaactcttacccttaagatagaagattttttcataagaaaaagagcacttggctcttttctgtt Intra-Species Hit: Count: 1 Min: 1 Max: 160 Len: 160 Subject: smit_SMT_1341_SMT_1342|putative cell division protein:protease maturation protein, putative|NEGATIVE:NEGATIVE|[1319206,1319365]|160 HSP 1 e-value: 2.0E-73 bit: 276.0 Len: 160 Query Start:1 Query End:160 Subject Strand: POSITIVE Subject Start: 1 Subject End: 160 agtatcaccaatctaatgagaacaaaagaaattattcccaaattccgtttattttctagttttgattgctattataccattttttccgaaagnnnnnnnctcttacccttaagatagaagattttttcataagaaaaagagcacttggctcttttctgtt agtatcaccaatctaatgagaacaaaagaaattattcccaaattccgtttattttctagttttgattgctattataccattttttccgaaagaaaaaaactcttacccttaagatagaagattttttcataagaaaaagagcacttggctcttttctgtt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUAUCACCAAUCUAAUGAGAACAAAAGAAAUUAUUCCCAAAUUCCGUUUAUUUUCUAGUUUUGAUUGCUAUUAUACCAUUUUUUCCGAAAGAAAAAAACUCUUACCCUUAAGAUAGAAGAUUUUUUCAUAAGAAAAAGAGCACUUGGCUCUUUUCUGUU ...........(((.(((((((.....(((....))).............((((((((.....................((((((.(....)))))))..(((((....))))))))))))).))))))).)))((((((((.....))))))))..... (-23.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACAGAAAAGAGCCAAGUGCUCUUUUUCUUAUGAAAAAAUCUUCUAUCUUAAGGGUAAGAGUUUUUUUCUUUCGGAAAAAAUGGUAUAAUAGCAAUCAAAACUAGAAAAUAAACGGAAUUUGGGAAUAAUUUCUUUUGUUCUCAUUAGAUUGGUGAUACU ....(((((((((.....))))))))).....(((((((.((..((((.....))))..)).))))))).............(((((..((.(((((.......(........)......(((((((((......)))))))))...))))).))))))) (-33.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 757 50 1 141 92 smit:SMT_1341|5end_putative_cell_division_protein_1319115..1319345_NEGATIVE 298 124 81 222 182 smit:SMT_1024|5end_transcriptional_regulator,_MarR_family_959404..959634_NEGATIVE 294 92 52 159 116 smit:SMT_1719|5end_DNA_mismatch_repair_protein_1706842..1707072_NEGATIVE 291 90 42 49 10 smit:SMT_1510|5end_ribose_5-phosphate_isomerase_1493488..1493718_NEGATIVE 281 159 111 121 71 smit:SMT_884|5end_hypothetical_protein_predicted_by_Glimmer/Critica_832306..832536_NEGATIVE 280 86 41 137 97 smit:SMT_1106|5end_ribonuclease_P_protein_component_1047972..1048202_NEGATIVE 272 84 41 133 85 smit:SMT_208|5end_leucyl-tRNA_synthetase_139977..140207_POSITIVE 270 70 33 137 100 smit:SMT_171|5end_50S_ribosomal_protein_L4_115557..115787_POSITIVE 262 95 58 152 109 smit:SMT_861|5end_adenine_phosphoribosyltransferase_805705..805935_NEGATIVE 253 131 98 225 194 smit:SMT_916|5end_3-hydroxy-3-methylglutaryl-CoA_reductase_870004..870234_NEGATIVE 239 89 44 132 97 smit:SMT_465|5end_oxidoreductase,_short_chain_dehydrogenase/reductase_family_protein_403244..403474_POSITIVE 239 89 44 132 97 smit:SMT_417|5end_short_chain_dehydrogenase_360373..360603_POSITIVE 237 77 34 164 115 smit:SMT_534|5end_hypothetical_protein_predicted_by_Glimmer/Critica_453571..453801_POSITIVE 237 77 34 164 115 smit:SMT_531|5end_hypothetical_protein_predicted_by_Glimmer/Critica_452672..452902_POSITIVE 237 77 34 164 115 smit:SMT_527|5end_hypothetical_protein_predicted_by_Glimmer/Critica_451502..451732_POSITIVE 237 77 34 164 115 smit:SMT_511|5end_hypothetical_protein_predicted_by_Glimmer/Critica_439391..439621_POSITIVE 234 108 69 124 85 smit:SMT_954|5end_cation-transporting_ATPase,_E1-E2_family_904025..904255_NEGATIVE 228 86 43 145 95 smit:SMT_1258|5end_acetolactate_synthase_large_subunit_1224866..1225096_NEGATIVE 223 87 43 63 19 smit:SMT_325|5end_K02029_polar_amino_acid_transport_system_permease_protein_263023..263253_NEGATIVE 222 87 45 105 66 smit:SMT_1096|5end_glyceraldehyde-3-phosphate_dehydrogenase_1038376..1038606_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 298 124 81 76 36 smit:SMT_1025|3end_hypothetical_protein_959550..959700_NEGATIVE 281 159 111 87 37 smit:SMT_885|3end_single-stranded-DNA-specific_exonuclease_RecJ_832340..832490_NEGATIVE 280 86 41 120 80 smit:SMT_1107|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1047989..1048139_NEGATIVE 270 70 33 112 75 smit:SMT_170|3end_50S_ribosomal_protein_L3_115612..115762_POSITIVE 253 79 31 74 29 smit:SMT_482|3end_hypothetical_protein_predicted_by_Glimmer/Critica_418414..418564_NEGATIVE 249 89 43 141 84 smit:SMT_548|3end_K02035_peptide/nickel_transport_system_substrate-binding_protein_467039..467189_POSITIVE 242 160 115 90 47 smit:SMT_342|3end_chaperone_protein_DnaJ_283016..283166_POSITIVE 239 89 44 111 76 smit:SMT_464|3end_ribonuclease_Z_403303..403453_POSITIVE 239 89 44 111 76 smit:SMT_416|3end_ribonuclease_Z_360432..360582_POSITIVE 237 77 34 50 1 smit:SMT_533|3end_RNA_binding_protein_453537..453687_POSITIVE 237 77 34 50 1 smit:SMT_530|3end_RNA_binding_protein_452638..452788_POSITIVE 237 77 34 50 1 smit:SMT_526|3end_RNA_binding_protein_451468..451618_POSITIVE 237 77 34 50 1 smit:SMT_510|3end_hypothetical_protein_439357..439507_POSITIVE 234 79 45 40 5 smit:SMT_481|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_417641..417791_NEGATIVE 224 158 122 140 96 smit:SMT_1494|3end_nucleoside_diphosphate_kinase_1480221..1480371_POSITIVE 221 65 24 94 46 smit:SMT_1131|3end_threonine_synthase_1065271..1065421_NEGATIVE 220 88 43 145 78 smit:SMT_1032|3end_putative_biotin_operon_repressor_969759..969909_POSITIVE 216 153 113 79 35 smit:SMT_477|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_414232..414382_NEGATIVE 215 70 22 54 10 smit:SMT_726|3end_ImpB/MucB/SamB_family_protein_630412..630562_NEGATIVE 214 157 111 72 22 smit:SMT_781|3end_hypothetical_protein_689509..689659_NEGATIVE