Origin IGS: aatgttcacttcctatattttttatgtaactaaattttacaattacatacgatatattataccatgcaatcgtattcatttcaaagaatttgaacattttctatatcatttgataatggaattttctttcgtttttaaaaattaaactgttgtaatatataatggtaaatatctttctacagaaagattaatcttggtttttaggcattatttttgatataataatcttaagtaaaattttattggaggtctt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aagacctccaataaaattttacttaagattattatatcaaaaataatgcctaaaaaccaagattaatctttctgtagaaagatatttaccattatatattacaacagtttaatttttaaaaacgaaagaaaattccattatcaaatgatatagaaaatgttcaaattctttgaaatgaatacgattgcatggtataatatatcgtatgtaattgtaaaatttagttacataaaaaatataggaagtgaacatt Mask Tandem Repeat Region ================================================ aatgttcacttcctatattttttatgtaactaaattttacaattacatacgatatattataccatgcaatcgtattcatttcaaagaatttgaacattttctatatcatttgataatggaattttctttcgtttttaaaaattaaactgttgtaatatataatggtaaatatctttctacagaaagattaatcttggtttttaggcattatttttgatataataatcttaagtaaaattttattggaggtctt Find is-nt database================================================ Query_seq: SMT_1325:SMT_1326|SMT_1325:SMT_1326:cell surface protein precursor:X-prolyl dipeptidyl peptidase:<-->:1300884..1301136 253 aatgttcacttcctatattttttatgtaactaaattttacaattacatacgatatattataccatgcaatcgtattcatttcaaagaatttgaacattttctatatcatttgataatggaattttctttcgtttttaaaaattaaactgttgtaatatataatggtaaatatctttctacagaaagattaatcttggtttttaggcattatttttgatataataatcttaagtaaaattttattggaggtctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1325:SMT_1326|SMT_1325:SMT_1326:cell surface protein precursor:X-prolyl dipeptidyl peptidase:<-->:1300884..1301136 253 aatgttcacttcctatattttttatgtaactaaattttacaattacatacgatatattataccatgcaatcgtattcatttcaaagaatttgaacattttctatatcatttgataatggaattttctttcgtttttaaaaattaaactgttgtaatatataatggtaaatatctttctacagaaagattaatcttggtttttaggcattatttttgatataataatcttaagtaaaattttattggaggtctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1325:SMT_1326|SMT_1325:SMT_1326:cell surface protein precursor:X-prolyl dipeptidyl peptidase:<-->:1300884..1301136 253 aatgttcacttcctatattttttatgtaactaaattttacaattacatacgatatattataccatgcaatcgtattcatttcaaagaatttgaacattttctatatcatttgataatggaattttctttcgtttttaaaaattaaactgttgtaatatataatggtaaatatctttctacagaaagattaatcttggtttttaggcattatttttgatataataatcttaagtaaaattttattggaggtctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatgttcacttcctatattttttatgtaactaaattttacaattacatacgatatattataccatgcaatcgtattcatttcaaagaatttgaacattttctatatcatttgataatggaattttctttcgtttttaaaaattaaactgttgtaatatataatggtaaatatctttctacagaaagattaatcttggtttttaggcattatttttgatataataatcttaagtaaaattttattggaggtctt Predict ORF larger than 30AA ================================================ Protein_Len: 47 Strand: + Start: 2 End: 142 . M F T S Y I F Y V T K F Y N Y I R Y I I P C N R I H F K E F E H F L Y H L I M E F S F V F K N ............................................................................................................... Protein_Len: 32 Strand: - Start: 2 End: 97 . H E S G I N K I Y S F K V I V Y S I N Y W A I T N M E F F K F M ............................................................................................................................................................ aatgttcacttcctatattttttatgtaactaaattttacaattacatacgatatattataccatgcaatcgtattcatttcaaagaatttgaacattttctatatcatttgataatggaattttctttcgtttttaaaaattaaactgttgtaatatataatggtaaatatctttctacagaaagattaatcttggtttttaggcattatttttgatataataatcttaagtaaaattttattggaggtctt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 90 Start: 56 End: 85 .......................................................TTTGAAATGAATACGATTGCATGGTATAAT........................................................................................................................................................................ PromScan Matrix: RpoD-17 Strand: + Score: 83 Start: 134 End: 163 .....................................................................................................................................TTTAAAAATTAAACTGTTGTAATATATAAT.......................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatgttcacttcctatattttttatgtaactaaattttacaattacatacgatatattataccatgcaatcgtattcatttcaaagaatttgaacattttctatatcatttgataatggaattttctttcgtttttaaaaattaaactgttgtaatatataatggtaaatatctttctacagaaagattaatcttggtttttaggcattatttttgatataataatcttaagtaaaattttattggaggtctt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1325:SMT_1326|SMT_1325:SMT_1326:cell surface protein precursor:X-prolyl dipeptidyl peptidase:<-->:1300884..1301136 253 aatgttcacttcctatattttttatgtaactaaattttacaattacatacgatatattataccatgcaatcgtattcatttcaaagaatttgaacattttctatatcatttgataatggaattttctttcgtttttaaaaattaaactgttgtaatatataatggtaaatatctttctacagaaagattaatcttggtttttaggcattatttttgatataataatcttaagtaaaattttattggaggtctt Intra-Species Hit: Count: 1 Min: 1 Max: 253 Len: 253 Subject: smit_SMT_1325_SMT_1326|cell surface protein precursor:X-prolyl dipeptidyl peptidase|NEGATIVE:POSITIVE|[1300884,1301136]|253 HSP 1 e-value: 1.0E-141 bit: 502.0 Len: 253 Query Start:1 Query End:253 Subject Strand: POSITIVE Subject Start: 1 Subject End: 253 aatgttcacttcctatattttttatgtaactaaattttacaattacatacgatatattataccatgcaatcgtattcatttcaaagaatttgaacattttctatatcatttgataatggaattttctttcgtttttaaaaattaaactgttgtaatatataatggtaaatatctttctacagaaagattaatcttggtttttaggcattatttttgatataataatcttaagtaaaattttattggaggtctt aatgttcacttcctatattttttatgtaactaaattttacaattacatacgatatattataccatgcaatcgtattcatttcaaagaatttgaacattttctatatcatttgataatggaattttctttcgtttttaaaaattaaactgttgtaatatataatggtaaatatctttctacagaaagattaatcttggtttttaggcattatttttgatataataatcttaagtaaaattttattggaggtctt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUGUUCACUUCCUAUAUUUUUUAUGUAACUAAAUUUUACAAUUACAUACGAUAUAUUAUACCAUGCAAUCGUAUUCAUUUCAAAGAAUUUGAACAUUUUCUAUAUCAUUUGAUAAUGGAAUUUUCUUUCGUUUUUAAAAAUUAAACUGUUGUAAUAUAUAAUGGUAAAUAUCUUUCUACAGAAAGAUUAAUCUUGGUUUUUAGGCAUUAUUUUUGAUAUAAUAAUCUUAAGUAAAAUUUUAUUGGAGGUCUU .......(((((((((((.....)))))...(((((((((......(((((((...............)))))))....(((((.....))))).......(((((((...((((((((((......)))....((((((((((((((((((((...)))))))))....(((((((....))))))).....))))))))))).)))))))..)))))))..........)))))))))....))))))... (-42.96) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGACCUCCAAUAAAAUUUUACUUAAGAUUAUUAUAUCAAAAAUAAUGCCUAAAAACCAAGAUUAAUCUUUCUGUAGAAAGAUAUUUACCAUUAUAUAUUACAACAGUUUAAUUUUUAAAAACGAAAGAAAAUUCCAUUAUCAAAUGAUAUAGAAAAUGUUCAAAUUCUUUGAAAUGAAUACGAUUGCAUGGUAUAAUAUAUCGUAUGUAAUUGUAAAAUUUAGUUACAUAAAAAAUAUAGGAAGUGAACAUU ...((.(((.(((.............(((((((((((((...((((((.((........))....(((((((....)))))))......))))))......(((..((.....(((((((..(....)....(((...((((....))))..))).............))))))).....))..)))..)))))))))).))).((((((((((.......)))))))))).....))).))).))....... (-38.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 719 110 61 201 152 smit:SMT_1325|5end_cell_surface_protein_precursor_1300793..1301023_NEGATIVE 288 135 93 94 45 smit:SMT_815|5end_hypothetical_protein_724294..724524_NEGATIVE 258 129 84 59 14 smit:SMT_344|5end_K02503_Hit-like_protein_involved_in_cell-cycle_regulation_283974..284204_NEGATIVE 246 103 62 176 133 smit:SMT_1250|5end_hypothetical_protein_1211345..1211575_NEGATIVE 228 137 91 194 156 smit:SMT_368|5end_hypothetical_protein_303662..303892_POSITIVE 219 210 184 165 136 smit:SMT_1087|5end_K01990_ABC-2_type_transport_system_ATP-binding_protein_1024165..1024395_NEGATIVE 207 165 126 181 137 smit:SMT_396|5end_putative_nicotinate_phosphoribosyltransferase_325062..325292_POSITIVE 203 86 56 149 116 smit:SMT_1992|5end_carboxynorspermidine_decarboxylase_1919207..1919437_NEGATIVE 203 86 56 149 116 smit:SMT_1415|5end_carboxynorspermidine_decarboxylase_1399570..1399800_NEGATIVE 202 200 160 81 49 smit:SMT_1167|5end_aminopeptidase_N_1115076..1115306_NEGATIVE 202 207 161 184 132 smit:SMT_382|5end_valyl-tRNA_synthetase_317706..317936_POSITIVE 197 210 163 143 95 smit:SMT_999|5end_hypothetical_protein_predicted_by_Glimmer/Critica_941642..941872_NEGATIVE 196 207 171 227 180 smit:SMT_1516|5end_K02029_polar_amino_acid_transport_system_permease_protein_1498172..1498402_NEGATIVE 194 103 61 161 119 smit:SMT_455|5end_cation_transport_ATPase_391670..391900_POSITIVE 193 227 202 141 119 smit:SMT_1272|5end_K02006_cobalt_transport_system_ATP-binding_protein_1236097..1236327_POSITIVE 193 208 170 173 133 smit:SMT_859|5end_K02086_DNA_replication_protein_804058..804288_NEGATIVE 188 90 46 49 14 smit:SMT_1796|5end_dihydroorotate_dehydrogenase_A_1770816..1771046_NEGATIVE 188 206 161 172 134 smit:SMT_311|5end_3-oxoacyl-_252123..252353_POSITIVE 187 217 194 207 183 smit:SMT_1947|5end_lysine_decarboxylase_1883113..1883343_NEGATIVE 186 136 95 66 22 smit:SMT_2042|5end_K04077_chaperonin_GroEL_(Hsp60)_1953889..1954119_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 257 135 98 46 4 smit:SMT_816|3end_SWF/SNF_family_helicase_724342..724492_NEGATIVE 239 230 193 59 24 smit:SMT_1877|3end_K06147_ATP-binding_cassette,_subfamily_B,_bacterial_1835261..1835411_POSITIVE 207 165 126 66 22 smit:SMT_395|3end_hypothetical_protein_325027..325177_POSITIVE 203 86 56 149 116 smit:SMT_1993|3end_hypothetical_protein_1919207..1919357_NEGATIVE 203 86 56 149 116 smit:SMT_1416|3end_hypothetical_protein_1399570..1399720_NEGATIVE 199 103 63 52 14 smit:SMT_1728|3end_exodeoxyribonuclease_VII_large_subunit_1714668..1714818_POSITIVE 194 103 61 151 109 smit:SMT_454|3end_hypothetical_protein_391740..391890_POSITIVE 193 227 202 144 122 smit:SMT_1271|3end_CDP-diacylglycerol--glycerol-3-phosphate_3-phosphatidyltransferase_1236180..1236330_POSITIVE 193 213 174 44 7 smit:SMT_1212|3end_acyl_carrier_protein,_putative_1171424..1171574_NEGATIVE 191 208 164 120 75 smit:SMT_1475|3end_K02004_1458661..1458811_POSITIVE 189 148 108 118 73 smit:SMT_457|3end_hypothetical_protein_396593..396743_POSITIVE 188 206 161 150 112 smit:SMT_310|3end_3-oxoacyl-_252181..252331_POSITIVE 185 217 192 140 112 smit:SMT_1299|3end_MutT/NUDIX_family_protein_1262572..1262722_POSITIVE 185 132 95 132 85 smit:SMT_374|3end_hypothetical_protein_311919..312069_POSITIVE 184 207 172 32 3 smit:SMT_531|3end_hypothetical_protein_predicted_by_Glimmer/Critica_453075..453225_POSITIVE 180 206 161 78 34 smit:SMT_1511|3end_hypothetical_protein_1493902..1494052_POSITIVE 177 95 55 55 18 smit:SMT_769|3end_spermidine/putrescine-binding_periplasmic_protein_679519..679669_NEGATIVE 176 72 39 125 86 smit:SMT_862|3end_hypothetical_protein_predicted_by_Glimmer/Critica_809191..809341_POSITIVE 175 187 163 117 88 smit:SMT_1936|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1874189..1874339_POSITIVE 175 218 172 68 11 smit:SMT_593|3end_K02030_polar_amino_acid_transport_system_substrate-binding_protein_510317..510467_POSITIVE