Origin IGS: tataattgaaagcgtaaaactttcttagttttatagttcttagcgcacattttatagattcaaaatataaaaaacaatgaaattttaggaaggcgatgtttt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaaacatcgccttcctaaaatttcattgttttttatattttgaatctataaaatgtgcgctaagaactataaaactaagaaagttttacgctttcaattata Mask Tandem Repeat Region ================================================ tataattgaaagcgtaaaactttcttagttttatagttcttagcgcacattttatagattcaaaatataaaaaacaatgaaattttaggaaggcgatgtttt Find is-nt database================================================ Query_seq: SMT_1318:SMT_1319|SMT_1318:SMT_1319:hypothetical protein predicted by Glimmer/Critica:muscus binding protein:->->:1284902..1285003 102 tataattgaaagcgtaaaactttcttagttttatagttcttagcgcacattttatagattcaaaatataaaaaacaatgaaattttaggaaggcgatgtttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1318:SMT_1319|SMT_1318:SMT_1319:hypothetical protein predicted by Glimmer/Critica:muscus binding protein:->->:1284902..1285003 102 tataattgaaagcgtaaaactttcttagttttatagttcttagcgcacattttatagattcaaaatataaaaaacaatgaaattttaggaaggcgatgtttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_1318:SMT_1319|SMT_1318:SMT_1319:hypothetical protein predicted by Glimmer/Critica:muscus binding protein:->->:1284902..1285003 102 tataattgaaagcgtaaaactttcttagttttatagttcttagcgcacattttatagattcaaaatataaaaaacaatgaaattttaggaaggcgatgtttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tataattgaaagcgtaaaactttcttagttttatagttcttagcgcacattttatagattcaaaatataaaaaacaatgaaattttaggaaggcgatgtttt Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: - Start: 2 End: 97 . Y N F A Y F K R L K I T R L A C K I S E F Y L F C H F K L F A M ..... tataattgaaagcgtaaaactttcttagttttatagttcttagcgcacattttatagattcaaaatataaaaaacaatgaaattttaggaaggcgatgtttt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tataattgaaagcgtaaaactttcttagttttatagttcttagcgcacattttatagattcaaaatataaaaaacaatgaaattttaggaaggcgatgtttt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_1318:SMT_1319|SMT_1318:SMT_1319:hypothetical protein predicted by Glimmer/Critica:muscus binding protein:->->:1284902..1285003 102 tataattgaaagcgtaaaactttcttagttttatagttcttagcgcacattttatagattcaaaatataaaaaacaatgaaattttaggaaggcgatgtttt Intra-Species Hit: Count: 1 Min: 1 Max: 102 Len: 102 Subject: smit_SMT_1318_SMT_1319|hypothetical protein predicted by Glimmer/Critica:muscus binding protein|POSITIVE:POSITIVE|[1284902,1285003]|102 HSP 1 e-value: 2.0E-51 bit: 202.0 Len: 102 Query Start:1 Query End:102 Subject Strand: POSITIVE Subject Start: 1 Subject End: 102 tataattgaaagcgtaaaactttcttagttttatagttcttagcgcacattttatagattcaaaatataaaaaacaatgaaattttaggaaggcgatgtttt tataattgaaagcgtaaaactttcttagttttatagttcttagcgcacattttatagattcaaaatataaaaaacaatgaaattttaggaaggcgatgtttt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUAAUUGAAAGCGUAAAACUUUCUUAGUUUUAUAGUUCUUAGCGCACAUUUUAUAGAUUCAAAAUAUAAAAAACAAUGAAAUUUUAGGAAGGCGAUGUUUU ........(((((((....((((((.((((((((.(((...........(((((((.........)))))))))).))))))))..))))))...))))))) (-15.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAACAUCGCCUUCCUAAAAUUUCAUUGUUUUUUAUAUUUUGAAUCUAUAAAAUGUGCGCUAAGAACUAUAAAACUAAGAAAGUUUUACGCUUUCAAUUAUA ...........................(((((((((((((((......)))))))))....))))))...........((((((.....))))))....... (-10.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 231 53 12 181 144 smit:SMT_1351|5end_dephospho-CoA_kinase_1329817..1330047_NEGATIVE 220 53 11 196 157 smit:SMT_824|5end_asparaginyl-tRNA_synthetase_748393..748623_NEGATIVE 219 47 11 158 127 smit:SMT_157|5end_hypothetical_protein_103525..103755_POSITIVE 192 57 12 137 88 smit:SMT_1954|5end_6-phospho-beta-glucosidase_1888548..1888778_POSITIVE 191 46 11 205 170 smit:SMT_794|5end_hypothetical_protein_703487..703717_NEGATIVE 190 57 11 66 30 smit:SMT_1867|5end_peptide_chain_release_factor_1_1828218..1828448_NEGATIVE 188 46 10 158 116 smit:SMT_1940|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1876589..1876819_POSITIVE 187 47 11 213 171 smit:SMT_899|5end_5,10-methylenetetrahydrofolate_reductase_848263..848493_NEGATIVE 184 53 13 188 147 smit:SMT_1697|5end_hypothetical_protein_1678846..1679076_POSITIVE 181 46 12 124 94 smit:SMT_394|5end_K07729_putative_transcriptional_regulator_324260..324490_POSITIVE 179 50 5 77 41 smit:SMT_1284|5end_predicted_amidophosphoribosyltransferase_1246962..1247192_POSITIVE 179 48 6 110 53 smit:SMT_1273|5end_K02006_cobalt_transport_system_ATP-binding_protein_1236909..1237139_POSITIVE 179 56 20 153 106 smit:SMT_822|5end_putative_mucus_binding_protein_740574..740804_NEGATIVE 178 100 58 217 169 smit:SMT_421|5end_K02003_363031..363261_POSITIVE 178 46 2 71 36 smit:SMT_103|5end_adenylosuccinate_synthetase_40766..40996_POSITIVE 175 33 6 55 32 smit:SMT_595|5end_phosphoglycerate_mutase_512593..512823_NEGATIVE 174 97 73 210 188 smit:SMT_1996|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1921078..1921308_NEGATIVE 172 55 12 91 48 smit:SMT_1434|5end_neopullulanase_1419645..1419875_NEGATIVE 171 44 7 56 24 smit:SMT_1794|5end_SOS_responce_UmuC_protein_1769074..1769304_NEGATIVE 169 44 6 211 176 smit:SMT_1153|5end_alpha-mannosidase_1098117..1098347_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 219 47 11 71 40 smit:SMT_156|3end_hypothetical_protein_103518..103668_POSITIVE 207 59 23 96 58 smit:SMT_539|3end_sensor_histidine_kinase_CiaH_456059..456209_POSITIVE 190 57 11 69 33 smit:SMT_1868|3end_thymidine_kinase_1828215..1828365_NEGATIVE 188 44 18 44 14 smit:SMT_1036|3end_alpha-galactosidase_978160..978310_NEGATIVE 187 47 11 109 67 smit:SMT_900|3end_5-methyltetrahydropteroyltriglutamate--_homocysteine_methyltransferase_848367..848517_NEGATIVE 179 50 5 80 44 smit:SMT_1283|3end_K02240_competence_protein_ComFA_1247045..1247195_POSITIVE 179 48 6 125 68 smit:SMT_1272|3end_K02006_cobalt_transport_system_ATP-binding_protein_1237004..1237154_POSITIVE 178 100 58 88 40 smit:SMT_420|3end_hypothetical_protein_predicted_by_Glimmer/Critica_362982..363132_POSITIVE 175 33 6 58 35 smit:SMT_596|3end_K03976_putative_transcription_regulator_512590..512740_NEGATIVE 169 67 22 140 88 smit:SMT_688|3end_carbamoyl-phosphate_synthase_small_chain_599576..599726_NEGATIVE 168 44 12 95 65 smit:SMT_2056|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1966735..1966885_POSITIVE 165 47 7 129 86 smit:SMT_1532|3end_hypothetical_protein_1515994..1516144_NEGATIVE 165 46 7 107 71 smit:SMT_1178|3end_choline_binding_protein_A_1135442..1135592_NEGATIVE 164 55 19 47 9 smit:SMT_1443|3end_23S_rRNA_m(5)U_1939_methyltransferase_1426503..1426653_NEGATIVE 163 57 11 122 68 smit:SMT_1653|3end_hydrolase_1642226..1642376_POSITIVE 162 52 20 107 80 smit:SMT_1092|3end_transcription_antitermination_factor_1033160..1033310_NEGATIVE 160 46 5 149 112 smit:SMT_2014|3end_tryptophan_synthase_beta_chain_1932727..1932877_POSITIVE 160 30 5 118 90 smit:SMT_436|3end_amidase_related_to_nicotinamidase_377575..377725_NEGATIVE 160 46 3 58 13 smit:SMT_292|3end_dTDP-glucose_4,6-dehydratase_235933..236083_POSITIVE 158 46 5 47 11 smit:SMT_917|3end_hydroxymethylglutaryl-CoA_synthase_870004..870154_NEGATIVE