Origin IGS: ggtcttcctctttcatttcctgtaagtctgagcttggaaggctagaaagagcgttcataaaacgttcatggctacagtcacattggaaacggatttcttcttcagaaagacgcttgtaggcctcgtcaccgtagatagccttgaggagggcttcgatatggtcgtcgctttccagaagggttgagatagctggcatttcttggatgcgtttttcaaagcgagcaatctcttcttccttagctcctggcaagacttgaactaggaaaccacctgcaaccttgaccttgtcttcttcgtctaaaaggacattgaggccgactgctgaaggggtttgttggctttcggtcaggtaaaaggccaggtcttcaccgatttccccagagatgaggggagtcatagagttgtaaggatttccagtaccgtagtctgtgataacgaggaattgaccatttccaacaaaaggtccaactaggacttcaccagttgcagtctttttgatgtcaacaccaggattttgaacgtagcctttgacgttccccttggtatcagcaacggtgataatggctcctagagagctagatcccagtaccttcactgttagtttggtatttcctttttcattggctgcgagaatctggctagcgataagagttcgaccaagcgctacagttgagctagcttgggtttggtgtttttcttgagcagtgcggacggtttctgtgctatcaaggacaaaagcacgaaaggctccgctttctgatattgttttaataattttatccatagctactattttagcataaaaatgcccaaagggggagccgtgtgtttgctgatttttaggataatggaccaggaaatcagcatgaaaataaaaagagaaacagattatttcagcatttgtaagatttatgctatgcttaaggtagaaaatgaaaggggtaacaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttgttacccctttcattttctaccttaagcatagcataaatcttacaaatgctgaaataatctgtttctctttttattttcatgctgatttcctggtccattatcctaaaaatcagcaaacacacggctccccctttgggcatttttatgctaaaatagtagctatggataaaattattaaaacaatatcagaaagcggagcctttcgtgcttttgtccttgatagcacagaaaccgtccgcactgctcaagaaaaacaccaaacccaagctagctcaactgtagcgcttggtcgaactcttatcgctagccagattctcgcagccaatgaaaaaggaaataccaaactaacagtgaaggtactgggatctagctctctaggagccattatcaccgttgctgataccaaggggaacgtcaaaggctacgttcaaaatcctggtgttgacatcaaaaagactgcaactggtgaagtcctagttggaccttttgttggaaatggtcaattcctcgttatcacagactacggtactggaaatccttacaactctatgactcccctcatctctggggaaatcggtgaagacctggccttttacctgaccgaaagccaacaaaccccttcagcagtcggcctcaatgtccttttagacgaagaagacaaggtcaaggttgcaggtggtttcctagttcaagtcttgccaggagctaaggaagaagagattgctcgctttgaaaaacgcatccaagaaatgccagctatctcaacccttctggaaagcgacgaccatatcgaagccctcctcaaggctatctacggtgacgaggcctacaagcgtctttctgaagaagaaatccgtttccaatgtgactgtagccatgaacgttttatgaacgctctttctagccttccaagctcagacttacaggaaatgaaagaggaagacc Mask Tandem Repeat Region ================================================ ggtcttcctctttcatttcctgtaagtctgagcttggaaggctagaaagagcgttcataaaacgttcatggctacagtcacattggaaacggatttcttcttcagaaagacgcttgtaggcctcgtcaccgtagatagccttgaggagggcttcgatatggtcgtcgctttccagaagggttgagatagctggcatttcttggatgcgtttttcaaagcgagcaatctcttcttccttagctcctggcaagacttgaactaggaaaccacctgcaaccttgaccttgtcttcttcgtctaaaaggacattgaggccgactgctgaaggggtttgttggctttcggtcaggtaaaaggccaggtcttcaccgatttccccagagatgaggggagtcatagagttgtaaggatttccagtaccgtagtctgtgataacgaggaattgaccatttccaacaaaaggtccaactaggacttcaccagttgcagtctttttgatgtcaacaccaggattttgaacgtagcctttgacgttccccttggtatcagcaacggtgataatggctcctagagagctagatcccagtaccttcactgttagtttggtatttcctttttcattggctgcgagaatctggctagcgataagagttcgaccaagcgctacagttgagctagcttgggtttggtgtttttcttgagcagtgcggacggtttctgtgctatcaaggacaaaagcacgaaaggctccgctttctgatattgttttaataattttatccatagctactattttagcataaaaatgcccaaagggggagccgtgtgtttgctgatttttaggataatggaccaggaaatcagcatgaaaataaaaagagaaacagattatttcagcatttgtaagatttatgctatgcttaaggtagaaaatgaaaggggtaacaa Find is-nt database================================================ Query_seq: SMT_1303:SMT_1304|SMT_1303:SMT_1304:tRNA-dihydrouridine synthase:hypothetical protein:<-->:1265822..1266769 948 ggtcttcctctttcatttcctgtaagtctgagcttggaaggctagaaagagcgttcataaaacgttcatggctacagtcacattggaaacggatttcttcttcagaaagacgcttgtaggcctcgtcaccgtagatagccttgaggagggcttcgatatggtcgtcgctttccagaagggttgagatagctggcatttcttggatgcgtttttcaaagcgagcaatctcttcttccttagctcctggcaagacttgaactaggaaaccacctgcaaccttgaccttgtcttcttcgtctaaaaggacattgaggccgactgctgaaggggtttgttggctttcggtcaggtaaaaggccaggtcttcaccgatttccccagagatgaggggagtcatagagttgtaaggatttccagtaccgtagtctgtgataacgaggaattgaccatttccaacaaaaggtccaactaggacttcaccagttgcagtctttttgatgtcaacaccaggattttgaacgtagcctttgacgttccccttggtatcagcaacggtgataatggctcctagagagctagatcccagtaccttcactgttagtttggtatttcctttttcattggctgcgagaatctggctagcgataagagttcgaccaagcgctacagttgagctagcttgggtttggtgtttttcttgagcagtgcggacggtttctgtgctatcaaggacaaaagcacgaaaggctccgctttctgatattgttttaataattttatccatagctactattttagcataaaaatgcccaaagggggagccgtgtgtttgctgatttttaggataatggaccaggaaatcagcatgaaaataaaaagagaaacagattatttcagcatttgtaagatttatgctatgcttaaggtagaaaatgaaaggggtaacaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_1303:SMT_1304|SMT_1303:SMT_1304:tRNA-dihydrouridine synthase:hypothetical protein:<-->:1265822..1266769 948 ggtcttcctctttcatttcctgtaagtctgagcttggaaggctagaaagagcgttcataaaacgttcatggctacagtcacattggaaacggatttcttcttcagaaagacgcttgtaggcctcgtcaccgtagatagccttgaggagggcttcgatatggtcgtcgctttccagaagggttgagatagctggcatttcttggatgcgtttttcaaagcgagcaatctcttcttccttagctcctggcaagacttgaactaggaaaccacctgcaaccttgaccttgtcttcttcgtctaaaaggacattgaggccgactgctgaaggggtttgttggctttcggtcaggtaaaaggccaggtcttcaccgatttccccagagatgaggggagtcatagagttgtaaggatttccagtaccgtagtctgtgataacgaggaattgaccatttccaacaaaaggtccaactaggacttcaccagttgcagtctttttgatgtcaacaccaggattttgaacgtagcctttgacgttccccttggtatcagcaacggtgataatggctcctagagagctagatcccagtaccttcactgttagtttggtatttcctttttcattggctgcgagaatctggctagcgataagagttcgaccaagcgctacagttgagctagcttgggtttggtgtttttcttgagcagtgcggacggtttctgtgctatcaaggacaaaagcacgaaaggctccgctttctgatattgttttaataattttatccatagctactattttagcataaaaatgcccaaagggggagccgtgtgtttgctgatttttaggataatggaccaggaaatcagcatgaaaataaaaagagaaacagattatttcagcatttgtaagatttatgctatgcttaaggtagaaaatgaaaggggtaacaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 2 Max: 781 Len: 780 Subject: gi|89110609|ref|AP_004389.1| heat shock protein Hsp33 [Escherichia coli W3110] HSP 1 e-value: 1.0E-14 bit: 77.4 Len: 780 Query Start:2 Query End:781 Subject Strand: null Subject Start: 5 Subject End: 256 . D K I I K T I S E S G A F R A F V L D S T E T V R T A Q E K H Q T Q A S S T V A L G R T L I A S Q I L A A N E K G N T K L T V K V L G S S S L G A I I T V A D T K G N V K G Y V Q N P G V D I K K T A T G E V L V G P F V G N G Q F L V I T D Y G T G N P Y N S M T P L I S G E I G E D L A F Y L T E S Q Q T P S A V G L N V L L D E E D K V K V A G G F L V Q V L P G A K E E E I A R F E K R I Q E M P A I S T L L E S D D H I E A L - - - - - - - L K A I Y G D E A Y K R L S E E E I R F Q C D C S H E R F M N A L S S L P S S D L Q E M K E E D ....................................................................................................................................................................... . D Q L H R Y L F E N F A V R G E L V T V S E T L Q Q I L E N H D Y P Q P V K N V L A E L L V A T S L L T A T L K F D G D I T V Q L Q G D G P M N L A V I N G N N N Q Q M R G V A R V Q G - E I P E N A D L K T L V G - - - - N G Y V V I T I T P S E G E R Y Q G V V G L E G D T L A A C L E D Y F M R S E Q L P T R - - L F I R T G D V D G K P A A G G M L L Q V M P A Q N A Q Q - - - - - - - - D D F D H L A T L T E T I K T E E L L T L P A N E V L W R L Y H E E E V T V Y D P Q D V E F K C T C S R E R C A D A L K T L P D E E V D S I L A E D ....................................................................................................................................................................... Find nr database================================================ Query_seq: SMT_1303:SMT_1304|SMT_1303:SMT_1304:tRNA-dihydrouridine synthase:hypothetical protein:<-->:1265822..1266769 948 gnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncatagctactattttagcataaaaatgcccaaagggggagccgtgtgtttgctgatttttaggataatggaccaggaaatcagcatgaaaataaaaagagaaacagattatttcagcatttgtaagatttatgctatgcttaaggtagaaaatgaaaggggtaacaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncatagctactattttagcataaaaatgcccaaagggggagccgtgtgtttgctgatttttaggataatggaccaggaaatcagcatgaaaataaaaagagaaacagattatttcagcatttgtaagatttatgctatgcttaaggtagaaaatgaaaggggtaacaa Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 15 End: 116 .............. M S C K S E L G R L E R A F I K R S W L Q S H W K R I S S S E R R L ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 38 Strand: + Start: 68 End: 181 ................................................................... M A T V T L E T D F F F R K T L V G L V T V D S L E E G F D M V V A F Q K G ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 40 Strand: + Start: 135 End: 254 ...................................................................................................................................... M A L R R A S I W S S L S R R V E I A G I S W M R F S K R A I S S S L A P G K T ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 46 Strand: + Start: 332 End: 469 ........................................................................................................................................................................................................................................................................................................................................... M L A F G Q V K G Q V F T D F P R D E G S H R V V R I S S T V V C D N E E L T I S N K R S N ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 57 Strand: + Start: 528 End: 698 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M T F P L V S A T V I M A P R E L D P S T F T V S L V F P F S L A A R I W L A I R V R P S A T V E L A W V W C F S .......................................................................................................................................................................................................................................................... Protein_Len: 32 Strand: + Start: 705 End: 800 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M R T V S V L S R T K A R K A P L S D I V L I I L S I A T I L A .................................................................................................................................................... Protein_Len: 56 Strand: + Start: 779 End: 946 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M H S Y Y F S I K M P K G G A V C L L I F R I M D Q E I S M K I K R E T D Y F S I C K I Y A M L K V E N E R G N .. Protein_Len: 38 Strand: - Start: 667 End: 780 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... L Q A L K P K T N K K L L A S P K Q A I L S L L V F P E A K Q Y Q K L L K M ........................................................................................................................................................................ Protein_Len: 56 Strand: - Start: 58 End: 225 ......................................................... L V N M A V T V N S V S K K K L F V S T P R T V T S L R S S P K S I T T A K W F P Q S L Q C K K S A N K L A L M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 2 End: 934 . E S I T K I I K D M A V I K A Y F H G F P S G H T Q Q N K P Y H V L F D A H F Y F S F C I I E A N T L N I S H K L Y F M .............. gnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncatagctactattttagcataaaaatgcccaaagggggagccgtgtgtttgctgatttttaggataatggaccaggaaatcagcatgaaaataaaaagagaaacagattatttcagcatttgtaagatttatgctatgcttaaggtagaaaatgaaaggggtaacaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncatagctactattttagcataaaaatgcccaaagggggagccgtgtgtttgctgatttttaggataatggaccaggaaatcagcatgaaaataaaaagagaaacagattatttcagcatttgtaagatttatgctatgcttaaggtagaaaatgaaaggggtaacaa Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 69 HP_score: -3.1 Tail_Score: -4.8048 Start: 473 End: 491 Full_Region: AAAAGGTCCAACTAG GACTTCA CCAGT TGCAGTC TTTTTGATGTCAACA ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GACTTCACCAGTTGCAGTC......................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 44 HP_score: -3.3 Tail_Score: -2.94958 Start: 742 End: 757 Full_Region: CAAGGACAAAAGCAC GAAAG GCTCCG CTTTC TGATATTGTTTTAAT .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GAAAGGCTCCGCTTTC............................................................................................................................................................................................... TransTerm Strand: - Conf: 42 HP_score: -3.3 Tail_Score: -2.52606 Start: 742 End: 757 Full_Region: attaaaacaatatca gaaag cggagc ctttc gtgcttttgtccttg .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gaaagcggagcctttc............................................................................................................................................................................................... Find igs database================================================ Query_seq: SMT_1303:SMT_1304|SMT_1303:SMT_1304:tRNA-dihydrouridine synthase:hypothetical protein:<-->:1265822..1266769 948 gnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncatagctactattttagcataaaaatgcccaaagggggagccgtgtgtttgctgatttttaggataatggaccaggaaatcagcatgaaaataaaaagagaaacagattatttcagcatttgtaagatttatgctatgcttaaggtagaaaatgaaaggggtaacaa Intra-Species Hit: Count: 1 Min: 782 Max: 948 Len: 167 Subject: smit_SMT_1303_SMT_1304|tRNA-dihydrouridine synthase:hypothetical protein|NEGATIVE:POSITIVE|[1265822,1266769]|948 HSP 1 e-value: 3.0E-89 bit: 331.0 Len: 167 Query Start:782 Query End:948 Subject Strand: POSITIVE Subject Start: 782 Subject End: 948 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................catagctactattttagcataaaaatgcccaaagggggagccgtgtgtttgctgatttttaggataatggaccaggaaatcagcatgaaaataaaaagagaaacagattatttcagcatttgtaagatttatgctatgcttaaggtagaaaatgaaaggggtaacaa .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................catagctactattttagcataaaaatgcccaaagggggagccgtgtgtttgctgatttttaggataatggaccaggaaatcagcatgaaaataaaaagagaaacagattatttcagcatttgtaagatttatgctatgcttaaggtagaaaatgaaaggggtaacaa Inter-species Hit: Count: 3 Min: 785 Max: 948 Len: 164 Subject: NC_008533_SPD_2014_SPD_2015|hypothetical protein:chaperonin, 33 kDa|NEGATIVE:POSITIVE|[1992811,1992974]|164 HSP 1 e-value: 6.0E-78 bit: 293.0 Len: 164 Query Start:785 Query End:948 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 164 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................agctactattttagcataaaaatgcccaaagggggagccgtgtgtttgctgatttttaggataatggaccaggaaatcagcatgaaaataaaaagagaaacagattatttcagcatttgtaagatttatgctatgcttaaggtagaaaatgaaaggggtaacaa ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................agctactattttagcataaaaatgcccaaagggggagccgtgtgtttactgattttcaggataatggaccaggaaatcagcatgaaaataaaaagagaaacagattattttagcatttgtcagatttatgctatgcttaaggtagaaaatgaaaggggtaacaa Subject: NC_003098_spr1992_spr1993|hypothetical protein:Hsp33-like chaperonin|NEGATIVE:POSITIVE|[1985311,1985474]|164 HSP 1 e-value: 6.0E-78 bit: 293.0 Len: 164 Query Start:785 Query End:948 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 164 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................agctactattttagcataaaaatgcccaaagggggagccgtgtgtttgctgatttttaggataatggaccaggaaatcagcatgaaaataaaaagagaaacagattatttcagcatttgtaagatttatgctatgcttaaggtagaaaatgaaaggggtaacaa ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................agctactattttagcataaaaatgcccaaagggggagccgtgtgtttactgattttcaggataatggaccaggaaatcagcatgaaaataaaaagagaaacagattattttagcatttgtcagatttatgctatgcttaaggtagaaaatgaaaggggtaacaa Subject: NC_003028_SP_2187_SP_2188|hypothetical protein:Hsp33-like chaperonin|NEGATIVE:POSITIVE|[2107782,2107945]|164 HSP 1 e-value: 1.0E-75 bit: 285.0 Len: 164 Query Start:785 Query End:948 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 164 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................agctactattttagcataaaaatgcccaaagggggagccgtgtgtttgctgatttttaggataatggaccaggaaatcagcatgaaaataaaaagagaaacagattatttcagcatttgtaagatttatgctatgcttaaggtagaaaatgaaaggggtaacaa ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................agctactattttagcataaaaatgcccaaagggggagccgtgtgtttactgattttcaggataatggaccaggaaatcagcatgaaaataaaaagagaaacagattattttagcatttgtcagatttatgctatgcttaaggtagaaaatgaaagggataacaa Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGCUACUAUUUUAGCAUAAAAAUGCCCAAAGGGGGAGCCGUGUGUUUGCUGAUUUUUAGGAUAAUGGACCAGGAAAUCAGCAUGAAAAUAAAAAGAGAAACAGAUUAUUUCAGCAUUUGUAAGAUUUAUGCUAUGCUUAAGGUAGAAAAUGAAAGGGGUAACAA ..(((((....(((((((((....(((....)))..((.((((...((((((((((..((........))..))))))))))..............((((.......)))).))))..))....))))))))).......)))))................... (-34.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGUUACCCCUUUCAUUUUCUACCUUAAGCAUAGCAUAAAUCUUACAAAUGCUGAAAUAAUCUGUUUCUCUUUUUAUUUUCAUGCUGAUUUCCUGGUCCAUUAUCCUAAAAAUCAGCAAACACACGGCUCCCCCUUUGGGCAUUUUUAUGCUAAAAUAGUAGCU ..(((((............((......))..(((((((((..........((((((((.....)))))..............(((((((((...((........))...))))))))).......)))..(((....)))....))))))))).....))))). (-30.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 292 68 23 192 132 smit:SMT_281|5end_glycosyl_transferase,_group_1_223551..223781_POSITIVE 283 70 24 65 10 smit:SMT_1966|5end_DNA_polymerase_III_alpha_subunit_1897934..1898164_POSITIVE 278 63 23 48 8 smit:SMT_1489|5end_K06147_ATP-binding_cassette,_subfamily_B,_bacterial_1468892..1469122_POSITIVE 276 69 23 185 120 smit:SMT_1537|5end_hypothetical_protein_1522668..1522898_NEGATIVE 264 78 31 56 9 smit:SMT_1170|5end_acetyltransferase,_CysE/LacA/LpxA/NodL_family_1118126..1118356_NEGATIVE 264 54 14 179 138 smit:SMT_935|5end_UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-_diaminopimelate_ligase_887326..887556_POSITIVE 262 89 42 74 18 smit:SMT_1046|5end_chaperonin_GroEL_987904..988134_NEGATIVE 262 89 42 74 18 smit:SMT_1044|5end_chaperonin_GroEL_(HSP60_family)_987435..987665_NEGATIVE 260 68 23 139 99 smit:SMT_76|5end_DNA_repair_and_genetic_recombination_protein_2677..2907_POSITIVE 258 79 31 74 10 smit:SMT_1606|5end_glutamate_racemase_1595857..1596087_POSITIVE 257 75 38 126 88 smit:SMT_600|5end_ADP-ribose_pyrophosphatase_516307..516537_POSITIVE 255 159 112 180 126 smit:SMT_1528|5end_K03310_alanine_or_glycine:cation_symporter,_AGCS_family_1512667..1512897_NEGATIVE 247 48 1 81 20 smit:SMT_989|5end_hypothetical_protein_933548..933778_NEGATIVE 246 79 35 76 37 smit:SMT_1483|5end_GTP-binding_protein_1463676..1463906_NEGATIVE 246 79 35 76 37 smit:SMT_1408|5end_GTP-binding_protein_1387147..1387377_NEGATIVE 244 77 34 208 158 smit:SMT_538|5end_DNA-binding_response_regulator_CiaR_455337..455567_POSITIVE 243 77 32 180 135 smit:SMT_915|5end_sucrose_operon_repressor_867574..867804_POSITIVE 243 57 30 54 26 smit:SMT_216|5end_Holliday_junction_DNA_helicase_RuvB_148257..148487_POSITIVE 242 49 1 113 67 smit:SMT_2005|5end_pullulanase,_type_I_1926289..1926519_NEGATIVE 242 49 1 207 161 smit:SMT_1967|5end_dicarboxylate/amino_acid:cation_(Na+_or_H+)_symporter_(DAACS)_family_protein_1899683..1899913_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 298 46 2 140 90 smit:SMT_631|3end_glycerate_kinase_537307..537457_POSITIVE 278 63 23 58 18 smit:SMT_1488|3end_K06147_ATP-binding_cassette,_subfamily_B,_bacterial_1468982..1469132_POSITIVE 265 61 22 80 32 smit:SMT_1895|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1847402..1847552_POSITIVE 264 54 14 80 39 smit:SMT_934|3end_pyridine_nucleotide-disulfide_oxidoreductase_887307..887457_POSITIVE 262 89 42 58 2 smit:SMT_1047|3end_chaperonin,_10_kDa_987920..988070_NEGATIVE 262 89 42 58 2 smit:SMT_1045|3end_cochaperonin_GroES_987451..987601_NEGATIVE 260 68 23 136 96 smit:SMT_75|3end_hypothetical_protein_2754..2904_POSITIVE 257 75 38 116 78 smit:SMT_599|3end_glucosamine-1-phosphate_N-acetyltransferase_/_UDP-N-acetylglucosamine_pyrophosphorylase_516377..516527_POSITIVE 255 159 112 71 17 smit:SMT_1529|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1512776..1512926_NEGATIVE 247 48 1 71 10 smit:SMT_990|3end_hypothetical_protein_933558..933708_NEGATIVE 246 69 30 43 1 smit:SMT_1379|3end_thymidylate_kinase_1356800..1356950_NEGATIVE 242 49 1 78 32 smit:SMT_1956|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1889537..1889687_NEGATIVE 242 49 1 73 27 smit:SMT_387|3end_hypothetical_protein_predicted_by_Glimmer/Critica_321118..321268_POSITIVE 240 36 2 132 104 smit:SMT_1857|3end_hypothetical_protein_1821145..1821295_POSITIVE 240 62 30 91 56 smit:SMT_1102|3end_transketolase_1043029..1043179_NEGATIVE 239 65 26 146 94 smit:SMT_96|3end_hypothetical_protein_28884..29034_POSITIVE 238 80 31 68 13 smit:SMT_1066|3end_holo-(acyl_carrier_protein)_synthase_1005859..1006009_POSITIVE 237 138 101 91 37 smit:SMT_191|3end_adenylate_kinase_126463..126613_POSITIVE 236 60 16 60 20 smit:SMT_1474|3end_K02003_1455951..1456101_POSITIVE 236 49 1 141 81 smit:SMT_1029|3end_K02027_multiple_sugar_transport_system_substrate-binding_protein_964382..964532_NEGATIVE