Origin IGS: atactacctcttctagctgattttaataacagcttattttaaggttttttccttataatgtcaagtaaggttttgggctttcaagataaaaatgggaaagtcttgaaaattatgataaaatagtggaaggaaatctatacattggagaaaaact .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agtttttctccaatgtatagatttccttccactattttatcataattttcaagactttcccatttttatcttgaaagcccaaaaccttacttgacattataaggaaaaaaccttaaaataagctgttattaaaatcagctagaagaggtagtat Mask Tandem Repeat Region ================================================ atactacctcttctagctgattttaataacagcttattttaaggttttttccttataatgtcaagtaaggttttgggctttcaagataaaaatgggaaagtcttgaaaattatgataaaatagtggaaggaaatctatacattggagaaaaact Find is-nt database================================================ Query_seq: SMT_128:SMT_129|SMT_128:SMT_129:K03449 MFS transporter, CP family, cyanate transporter:putative glycosyltransferase:<-->:79959..80112 154 atactacctcttctagctgattttaataacagcttattttaaggttttttccttataatgtcaagtaaggttttgggctttcaagataaaaatgggaaagtcttgaaaattatgataaaatagtggaaggaaatctatacattggagaaaaact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_128:SMT_129|SMT_128:SMT_129:K03449 MFS transporter, CP family, cyanate transporter:putative glycosyltransferase:<-->:79959..80112 154 atactacctcttctagctgattttaataacagcttattttaaggttttttccttataatgtcaagtaaggttttgggctttcaagataaaaatgggaaagtcttgaaaattatgataaaatagtggaaggaaatctatacattggagaaaaact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_128:SMT_129|SMT_128:SMT_129:K03449 MFS transporter, CP family, cyanate transporter:putative glycosyltransferase:<-->:79959..80112 154 atactacctcttctagctgattttaataacagcttattttaaggttttttccttataatgtcaagtaaggttttgggctttcaagataaaaatgggaaagtcttgaaaattatgataaaatagtggaaggaaatctatacattggagaaaaact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atactacctcttctagctgattttaataacagcttattttaaggttttttccttataatgtcaagtaaggttttgggctttcaagataaaaatgggaaagtcttgaaaattatgataaaatagtggaaggaaatctatacattggagaaaaact Predict ORF larger than 30AA ================================================ atactacctcttctagctgattttaataacagcttattttaaggttttttccttataatgtcaagtaaggttttgggctttcaagataaaaatgggaaagtcttgaaaattatgataaaatagtggaaggaaatctatacattggagaaaaact Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 90 Start: 36 End: 65 ...................................CTTGACATTATAAGGAAAAAACCTTAAAAT......................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atactacctcttctagctgattttaataacagcttattttaaggttttttccttataatgtcaagtaaggttttgggctttcaagataaaaatgggaaagtcttgaaaattatgataaaatagtggaaggaaatctatacattggagaaaaact Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_128:SMT_129|SMT_128:SMT_129:K03449 MFS transporter, CP family, cyanate transporter:putative glycosyltransferase:<-->:79959..80112 154 atactacctcttctagctgattttaataacagcttattttaaggttttttccttataatgtcaagtaaggttttgggctttcaagataaaaatgggaaagtcttgaaaattatgataaaatagtggaaggaaatctatacattggagaaaaact Intra-Species Hit: Count: 1 Min: 1 Max: 154 Len: 154 Subject: smit_SMT_128_SMT_129|K03449 MFS transporter, CP family, cyanate transporter:putative glycosyltransferase|NEGATIVE:POSITIVE|[79959,80112]|154 HSP 1 e-value: 2.0E-82 bit: 305.0 Len: 154 Query Start:1 Query End:154 Subject Strand: POSITIVE Subject Start: 1 Subject End: 154 atactacctcttctagctgattttaataacagcttattttaaggttttttccttataatgtcaagtaaggttttgggctttcaagataaaaatgggaaagtcttgaaaattatgataaaatagtggaaggaaatctatacattggagaaaaact atactacctcttctagctgattttaataacagcttattttaaggttttttccttataatgtcaagtaaggttttgggctttcaagataaaaatgggaaagtcttgaaaattatgataaaatagtggaaggaaatctatacattggagaaaaact Inter-species Hit: Count: 1 Min: 5 Max: 133 Len: 129 Subject: NC_003028_SP_0101_SP_0102|transporter, putative:glycosyl transferase|NEGATIVE:POSITIVE|[103412,103561]|150 HSP 1 e-value: 9.0E-51 bit: 200.0 Len: 129 Query Start:5 Query End:133 Subject Strand: POSITIVE Subject Start: 5 Subject End: 133 ....tacctcttctagctgattttaataacagcttattttaaggttttttccttataatgtcaagtaaggttttgggctttcaagataaaaatgggaaagtcttgaaaattatgataaaatagtggaaggaaa..................... ....tacctcttctagctgattttaataacagcttattttaaggctttttcactataatgtcaagtaaggtttctggctttcaagataaaaatgggaaagtcttgaaaattatgatagaatggtggaaggaaa..................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACCUCUUCUAGCUGAUUUUAAUAACAGCUUAUUUUAAGGUUUUUUCCUUAUAAUGUCAAGUAAGGUUUUGGGCUUUCAAGAUAAAAAUGGGAAAGUCUUGAAAAUUAUGAUAAAAUAGUGGAAGGAAA .((((.....(((((..........))))).......))))(((((((((((..(((((..(((..(((.((((((((.............)))))))).)))..))))))))..)))).))))))).. (-27.52) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUCCUUCCACUAUUUUAUCAUAAUUUUCAAGACUUUCCCAUUUUUAUCUUGAAAGCCCAAAACCUUACUUGACAUUAUAAGGAAAAAACCUUAAAAUAAGCUGUUAUUAAAAUCAGCUAGAAGAGGUA (((((((.................(((((((((..............)))))))))..(((........))).......)))))))..(((((......(((((..........)))))....))))). (-20.34) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 779 100 51 195 146 smit:SMT_128|5end_K03449_MFS_transporter,_CP_family,_cyanate_transporter_79868..80098_NEGATIVE 316 98 56 89 35 smit:SMT_1107|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1048081..1048311_NEGATIVE 316 98 56 198 144 smit:SMT_1106|5end_ribonuclease_P_protein_component_1047972..1048202_NEGATIVE 284 99 54 191 140 smit:SMT_995|5end_K07574_putative_RNA-binding_protein_containing_KH_domain_936468..936698_NEGATIVE 244 74 31 121 71 smit:SMT_1612|5end_K06024_segregation_and_condensation_protein_B_1600052..1600282_POSITIVE 228 37 4 135 92 smit:SMT_1642|5end_K03497_chromosome_partitioning_protein,_ParB_family_1628996..1629226_NEGATIVE 217 84 60 61 39 smit:SMT_488|5end_dCMP_deaminase_422956..423186_POSITIVE 214 48 3 183 139 smit:SMT_1260|5end_hypothetical_protein_1225517..1225747_POSITIVE 212 96 58 88 48 smit:SMT_963|5end_K02015_iron_complex_transport_system_permease_protein_909632..909862_NEGATIVE 212 99 55 192 157 smit:SMT_434|5end_orotidine_5'-phosphate_decarboxylase_376107..376337_POSITIVE 211 59 11 95 44 smit:SMT_1963|5end_ribonuclease_BN,_putative_1896462..1896692_NEGATIVE 211 37 2 126 96 smit:SMT_1650|5end_ribonuclease_III_1636309..1636539_POSITIVE 210 93 54 215 181 smit:SMT_90|5end_DNA_polymerase_III_beta_subunit_21377..21607_POSITIVE 209 98 53 168 119 smit:SMT_545|5end_hypothetical_protein_predicted_by_Glimmer/Critica_461249..461479_POSITIVE 208 50 3 150 110 smit:SMT_1730|5end_geranyltranstransferase_1714775..1715005_POSITIVE 206 54 11 60 26 smit:SMT_1836|5end_cytidine/deoxycytidylate_deaminase_family_protein_1804498..1804728_NEGATIVE 206 46 7 198 166 smit:SMT_1517|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1498313..1498543_POSITIVE 205 88 41 226 174 smit:SMT_1365|5end_translation_initiation_factor_IF-3_1344485..1344715_NEGATIVE 204 100 55 206 154 smit:SMT_640|5end_PTS_system,_lactose-specific_IIBC_component_546790..547020_NEGATIVE 204 100 54 170 125 smit:SMT_133|5end_L-serine_dehydratase_beta_subunit_85237..85467_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 303 98 60 48 3 smit:SMT_1108|3end_acetate_kinase_1048122..1048272_NEGATIVE 244 74 31 102 52 smit:SMT_1611|3end_K05896_segregation_and_condensation_protein_A_1600113..1600263_POSITIVE 238 47 5 132 87 smit:SMT_1581|3end_transcriptional_regulator,_biotin_repressor_family_1573120..1573270_NEGATIVE 224 58 12 113 65 smit:SMT_1232|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1193209..1193359_POSITIVE 217 84 60 42 20 smit:SMT_487|3end_transcriptional_regulator,_TetR_family_423017..423167_POSITIVE 216 78 56 87 65 smit:SMT_1186|3end_hypothetical_protein_1144886..1145036_POSITIVE 210 93 54 56 22 smit:SMT_89|3end_chromosomal_replication_initiator_protein_21298..21448_POSITIVE 207 91 56 81 31 smit:SMT_1095|3end_ribosomal_large_subunit_pseudouridine_synthase,_RluD_subfamily_1037328..1037478_POSITIVE 205 47 5 92 48 smit:SMT_1729|3end_exodeoxyribonuclease_VII_small_subunit_1714858..1715008_POSITIVE 205 88 41 63 11 smit:SMT_1366|3end_K02238_competence_protein_ComEC_1344648..1344798_NEGATIVE 202 102 66 51 14 smit:SMT_2095|3end_K00783_hypothetical_protein_1990162..1990312_POSITIVE 202 100 55 88 32 smit:SMT_888|3end_K02030_polar_amino_acid_transport_system_substrate-binding_protein_836333..836483_POSITIVE 202 99 56 139 103 smit:SMT_803|3end_peptidoglycan_N-acetylglucosamine_deacetylase_712405..712555_NEGATIVE 200 80 37 90 45 smit:SMT_1470|3end_K06188_aquaporin_Z_1453266..1453416_POSITIVE 200 79 54 47 20 smit:SMT_230|3end_hypothetical_protein_predicted_by_Glimmer/Critica_165666..165816_NEGATIVE 198 45 1 101 58 smit:SMT_1895|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1847402..1847552_POSITIVE 196 70 29 100 59 smit:SMT_1859|3end_serine_protease_1822050..1822200_NEGATIVE 196 70 29 100 59 smit:SMT_1660|3end_serine_protease_1649852..1650002_NEGATIVE 196 70 29 100 59 smit:SMT_87|3end_serine_protease_18908..19058_POSITIVE 193 67 27 146 106 smit:SMT_1727|3end_uridine_kinase_1713201..1713351_POSITIVE